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- 대한음성언어의학회지= THE JOURNAL OF THE KOREAN SOCIETY OF LOGOPEDICS AND PHONIATRICS(1)
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구개봉합술 후 발생한 구비강누공의 폐쇄 후 말소리 결과 : 증례보고
서민교, 김다와, 김은주, 윤보근, 김성일, 임대호, 고승오, 문승영, 김현기, 신효근 대한구순구개열학회 대한구순구개열학회지 6 Pages
대한구순구개열학회 대한구순구개열학회지 2009, 제 12권 1호 1 1-6 (6 pages)
68.00 62.50 2008.1.30 45.50% 65.50 58.25 2009.2.2 2 nd fistula closure 2009.3.11 51.52% 53.1 51.3 Table 3. Variation of vowel formants Table 4. Variation of first formant energy Variations of vowel formants before and after operation vowels Hz 4,000 3,500 3,000 2,500 2,000 1,500 1,000 500 0 F1 F2 F3 F1 F2 F3 /o/ N Variations of first formant energy before and after operation vowels dB 16 14 12 10... -
Analysis of a Sphingosine 1-phosphate Receptor hS1P3 in Rat Hepatoma Cells
Dong-SoonIm 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.3 2 139-142 (4 pages)
Spiegel, 1993; Wu, 1995; An, 2000; Pebay, 2001). As shown in Fig. 2, S1P activated MAP kinases in hS1P 3 transformants about 2.5-fold from 5 min to 30 min, however, no activation in vector trans- formants or non-transfected RH7777 cells. The activation was PTX-sensitive (Fig. 3). Also, treatment with PTX (100 Fig. 1. Effect of S1P on cAMP accumulation in hS1P3 expressing RH7777 cells. In the... To examine intracellular signaling of human S1P3 (hS1P3), a sphingosine 1-phosphate (S1P) receptor in plasma membrane, hS1P3 DNA was transfected into RH7777 rat hepatoma cell line, and the inhibition of forskolin-induced cAMP accumulation and activation of MAP kinases by S1P were tested. In hS1P3 transformants, S1P inhibited forskolin-induced activation of adenylyl cyclase activity by about 80% and activated MAP kinases in dose-dependent and pertussis-toxin (PTX) sensitive manners. In oocytes... -
Overview of the Korean Longitudinal Study on Cognitive Aging and Dementia
Ji Won Han, Tae Hui Kim, Kyung Phil Kwak, Kayoung Kim, Bong Jo Kim, Shin Gyeom Kim, Jeong Lan Kim, Tae Hyun Kim, Seok Woo Moon, Jae Young 대한신경정신의학회 Psychiatry Investigation 8 Pages
대한신경정신의학회 Psychiatry Investigation 2018, 제 15권 제 8호 4 767-774 (8 pages)
Subjective Mem- ory Complaints Questionnaire (SMCQ) 17 to the subjects and the Korean version of the Short Informant Questionnaire on Cognitive Decline in the Elderly (SIQCODE-K) 18 to their in- formants to evaluate subjective complaints associated with cog- nitive impairment. Research neuropsychologists or trained research nurses administered the Korean version of the Con- sortium to Establish... Objective_Due to an unprecedented rate of population aging, South Korea is facing a dementia epidemic. For this reason, the Korean Longitudinal Study on Cognitive Aging and Dementia (KLOSCAD) was launched in 2009 with support from the Korean Health Industry Development Institute to investigate the epidemiology, biopsychosocial risk factors, and outcomes of dementia and dementia-related conditions. Methods_The KLOSCAD is the first nationwide multi-center population-based prospective cohort study.... -
한국판 성인용 자기 보고형 및 관찰자 보고형 Young ADHD 척도의 신뢰도 및 타당도 연구
김은경, 서신영, 김근향 대한신경정신의학회 신경정신의학 10 Pages
대한신경정신의학회 신경정신의학 2006, 제 45권 제 6호 12 578-587 (10 pages)
(K-YAQ-S) and the Young ADHD Questionnaire-Informant-Report (K-YAQ-I). Methods ：269 normal college students completed the K-YAQ-S and the Lee Ji-Yeon’s adult ADHD scale (1996). 213 in- formants of the subjects completed the K-YAQ-I. Results：The K-YAQ-S and the K-YAQ-I had good internal consistency (Cronbach’s = .957, .965). An ADHD symptoma- tology and emotional problem subscales of the... -
The Small GTPase CsRAC1 Is Important for Fungal Development and Pepper Anthracnose in Colletotrichum scovillei
Noh-Hyun Lee, Teng Fu, Jong-Hwan Shin, Yong-Won Song, Dong-Cheol Jang, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 10 607-618 (12 pages)
Materials and Methods Fungal strains, culture conditions. In this study, Col- ..PAGE:3 Roles of CsRAC1 in Anthracnose Disease 609 letotrichum scovillei wild-type strain KC05 and its trans- formants were routinely incubated on oatmeal agar (OMA; 50 g oatmeal and 15 g agar powder per liter) at 25 oC with continuous light (Fu et al., 2021). Mycelia for extraction of genomic DNA and total RNA were... The pepper anthracnose fungus, Colletotrichum scovil- lei, causes severe losses of pepper fruit production in the tropical and temperate zones. RAC1 is a highly conserved small GTP-binding protein in the Rho GT- Pase family. This protein has been demonstrated to play a role in fungal development, and pathogenicity in several plant pathogenic fungi. However, the functional roles of RAC1 are not characterized in C. scovillei caus- ing anthracnose on pepper fruits. Here, we generated a deletion... -
Cucumber Mosaic Virus 1a Protein Interacts with the Tobacco SHE1 Transcription Factor and Partitions between the Nucleus and the Tonoplast Membrane
Ju-Yeon Yoon, Peter Palukaitis 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 9 182-193 (12 pages)
between the CMV 1a protein and the SHE1 TF. CMV, cucumber mosaic vi- rus; TF, transcription factor; BD, binding domain; AD, activation domain. SD-LT and SD-LTHA represent assays in which trans- formants were selected on SD/-Trp/-Leu, and SD/-Trp/-Leu/- His/-Ade plates without X-gal to detect the initiation of transcrip- tion of amino acid biosynthesis reporter genes (TRP, LEU, HIS, and ADE).... The transcription factor SHE1 was identified as an interacting partner with the cucumber mosaic virus (CMV) 1a protein in the yeast two-hybrid system, by a pull-down assay, and via bimolecular fluorescent complementation. Using fluorescent-tagged proteins and confocal microscopy, the CMV 1a protein itself was found distributed predominantly between the nu- cleus and the tonoplast membrane, although it was also found in speckles in the cytoplasm. The SHE1 protein was localized in the nucleus, but... -
The Membrane-Bound Protein, MoAfo1, Is Involved in Sensing Diverse Signals from Different Surfaces in the Rice Blast Fungus
Md Abu Sadat, Joon-Hee Han, Seongbeom Kim, Yong-Hwan Lee, Kyoung Su Kim, Jaehyuk Choi 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 1 87-98 (12 pages)
by double-joint PCR where ~1 kb sequences of this gene were amplified and fused to hygromycin resistant gene (HPH). Then the construct was transferred to wildtype protoplasts and resulting trans- formants were primarily selected through PCR-based screening using specific primer pair MoAFO1_SF and MoAFO1_SR (Supplementary Table 1). Knock-out mutant was confirmed by Southern blot analysis usi... To establish an infection, fungal pathogens must recog- nize diverse signals from host surfaces. The rice blast fungus, Magnaporthe oryzae, is one of the best models studying host-pathogen interactions. This fungus recog- nizes physical or chemical signals from the host surfac- es and initiates the development of an infection struc- ture called appressorium. Here, we found that protein MoAfo1(appressorium formation, MGG_10422) was involved in sensing signal molecules such as cutin mono- mers and... -
A LysM Domain-Containing Protein LtLysM1 Is Important for Vegetative Growth and Pathogenesis in Woody Plant Pathogen Lasiodiplodia theobromae
Dulanjalee Lakmali Harishchandra, Wei Zhang, Xinghong Li, Kandawatte Wedaralalage Thilini Chethana, Kevin David Hyde, Siraprapa Brooks, Jiye Yan, Junbo Peng 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 4호 3 323-334 (12 pages)
of overexpressed and RNAi trans- formants of LtLysM1 gene. The LtLysM1 open reading frame (ORF) was amplified with primer pairs LysM1OE- f/LysM1OE-r (Table 1) and then subcloned down- stream of the PtrpC promoter in the modified pKSNTP vector. Afterwards, the fusion construct, referred to as pKSNTP:LtLysM1, was transformed into L. theobromae protoplast using polyethylene glycol (PEG)-mediated... Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence... -
A Histone Deacetylase, MoHDA1 Regulates Asexual Development and Virulence in the Rice Blast Fungus
Taehyun Kim, Song Hee Lee, Young Taek Oh, Junhyun Jeon 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 4호 2 314-322 (9 pages)
in the trans- formants, for which over-expression were confirmed by qRT-PCR (#85 and 86 in Fig. 4A). This result was highly reproducible throughout several rounds of independent experiments, strongly suggesting that it is not experimen- tal artefacts. When transcription level of MoCON6 and asexual spore production were compared across different strains, we were able to see the negative... Interplay between histone acetylation and deacetylation is one of the key components in epigenetic regulation of transcription. Here we report the requirement of Mo- HDA1-mediated histone deacetylation during asexual development and pathogenesis for the rice blast fungus, Magnaporthe oryzae. Structural similarity and phylogenetic analysis suggested that MoHDA1 is an ortholog of Saccharomyces cerevisiae Hda1, which is a representative member of class II histone deacetylases. Targeted deletion of... -
Survival Factor Gene FgSvf1 Is Required for Normal Growth and Stress Resistance in Fusarium graminearum
Taiying Li, Boknam Jung, Sook-Young Park, Jungkwan Lee 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 1 393-405 (13 pages)
of FgSvf1 was fused with a geneticin resistance cassette (GEN) amplified with pII99 (Namiki et al., 2001), through SMR. All of the PCR primers used in this study are listed in Table 1. Trans- formants were screened by PCR to check whether they car- ried the desired deletion, overexpression, and complemen- tation constructs. Protoplast production and transformation. For conidia production,... Survival factor 1 (Svf1) is a protein involved in cell survival pathways. In Saccharomyces cerevisiae, Svf1 is required for the diauxic growth shift and survival under stress conditions. In this study, we characterized the role of FgSvf1, the Svf1 homolog in the homothallic ascomycete fungus Fusarium graminearum. In the FgSvf1 deletion mutant, conidial germination was delayed, vegetative growth was reduced, and pathogenicity was completely abolished. Although the FgSvf1 deletion mutant produced...