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Korean red ginseng excitation of paraventricular nucleus neurons via non-N-methyl-D-aspartate glutamate receptor activation in mice
Yiming Shen, Janardhan P. Bhattarai, Soo Joung Park, Gyu Seung Lee, Pan Dong Ryu, Seong Kyu Han 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 3 172-178 (7 pages)
*p 0.05. and they show no low-threshold spikes after hyperpolarizing pulses larger than 100 mV [11]. The parvocellular neurons are composed of neurosecretory and preautonomic neurons and show discrete or bursting low-threshold spikes after hyperpolarizing pulses larger than 100 mV [11]. The parvocellular neurosecretory neurons synthesize regulatory hormones and their axons are involved in... a critical role in stress responses and hormone secretions. Although the action mechanisms of KRG on various cells and systems have been reported, the direct membrane effects of KRG on PVN neurons have not been fully described. In this study, the direct membrane effects of KRG on PVN neuronal activity were investigated by using a perforated patch-clamp in ICR mice. In gramicidin perforated patch-clamp mode, KRG extract (KRGE) induced repeatable depolarization followed by hyperpolarization of PVN... -
Histologic morphology and involucrin, filaggrin, and keratin expression in normal canine skin from dogs of different breeds and coat types
Sirin Theerawatanasirikul, Gunnaporn Suriyaphol, Roongroje Thanawongnuwech, Achariya Sailasuta* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 7 163-170 (8 pages)
digital areas. The tissues were then placed into 10% neutral buffer formalin for 24 h for routine histopathologic and immunohistochemical examination. Fresh skin samples were stored in RNAlater solution (Ambion, USA) at 20 o C until analysis. Histopathology and immunohistochemistry Skin samples were cut into sections (4∼5 m thickness), deparaffinized in xylene, rehydrated in graded ethanol,... the ventral and weight-bearing sites, and a relative increase of keratin 5 (p ＜ 0.05). q-PCR analysis showed that their the levels of mRNA were positively correlated with expression of the corresponding proteins in skin samples (p ＜ 0.05). The present study is the first to report the relationship between epidermal gene expression and histologic morphology of the skin in normal dogs. Further studies will be essential to fully understand the pathogenesis of skin barrier dysfunctions in canines. -
Effect of adrenalectomy on gene expression of adrenoceptor subtypes in the hypothalamic paraventricular nucleus
KyungYoonKam, SeungYubShin, SeongKyuHan, LongHuaLi, WoNeeChong, DaeHyunBaek, SoYeongLee, PanDongRyu 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2004, 제 44권 제 2호 8 207-215 (9 pages)
solution containing 0.9% saline and 5% dextrose. Animals were sacrificed by decapitation 7 days after surgery [8]. Brains were rapidly removed, frozen in isopentane cooled to -40 to -50 oC and stored at -70 oC. Trunk blood was collected and serum separated by centrifugation (3,000 g, 10 min). The sera were stored at -70 oC until corticosterone radioimmunoassay. Corticosterone radioimmunoassay The... -
The neuroprotective mechanism of ampicillin in a mouse model of transient forebrain ischemia
Kyung-EonLee, Kyung-OkCho, Yun-SikChoi, SeongYunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.2 7 185-192 (8 pages)
damage Three days after transient forebrain ischemia, the animals were transcardially perfused with 4% paraformaldehyde in 0.1 M phosphate buffer for 30 min, followed by 1.5 ml of latex solution to evaluate the plasticity of the posterior communicating artery. After the mice had been cooled on ice for 1 day, the brains were isolated and photographed with a digital camera (EOS 300D, Canon,... Ampicillin, a β-lactam antibiotic, dose-dependently protects neurons against ischemic brain injury. The present study was performed to investigate the neuroprotective mechanism of ampicillin in a mouse model of transient global forebrain ischemia. Male C57BL/6 mice were anesthetized with halothane and subjected to bilateral common carotid artery occlusion for 40 min. Before transient forebrain ischemia, ampicillin (200 mg/kg, intraperitoneally [i.p.]) or penicillin G (6,000 U/kg or 20,000 U/kg,... -
Mechanisms of Selective Antimicrobial Activity of Gaegurin 4
HeejeongKim, ByeongJaeLee, MunHanLee, SeongGeunHong, PanDongRyu 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.1 7 39-48 (10 pages)
) was very short (＜1 min) in the presence of GGN4, it was difficult to measure the conductances for multiple GGN4 concentrations using 100 mM KCl ion salt solution. Therefore, the membrane conductances at a single concentration of GGN4 were measured in given membranes formed with extracted lipids in 25 mM KCl solution. GGN4-induced conductances usually reached a steady-state at 7∼10 min... Gaegurin 4 (GGN4), an antimicrobial peptide isolated from a Korean frog, is five times more potent against Gram-positive than Gram-negative bacteria, but has little hemolytic activity. To understand the mechanism of such cell selectivity, we examined GGN4-induced K＋ efflux from target cells, and membrane conductances in planar lipid bilayers. The K＋ efflux from Gram-positive M. luteus (2.5</SUP>Ռg/ml) was faster and larger than that from Gram-negative E. coli (75Ռg/ml), while... -
Na+-Ca2+ Exchange Curtails Ca2+ before Its Diffusion to Global Ca2+i in the Rat Ventricular Myocyte
Sung-WanAhnChangMannKo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.2 4 95-102 (8 pages)
3 mg of protease (type XIV, Sigma) according to the method reported by Mitra and Morad (1985). The compositions of the Tyrode solution (in mmol/L) were 137 NaCl, 5.4 KCl, 10 HEPES, 1 MgCl 2 and 10 glu- cose, pH=7.4 at 37 o C. All the experiments were performed at room temperature. Current recording The rat ventricular myocytes were patch-clamped in a whole-cell configuration and held at 40 mV... In the heart, Na-Ca2 exchange (NCX) is the major Ca2 extrusion mechanism. NCX has been considered as a relaxation mechanism, as it reduces global [Ca2]i raised during activation. However, if NCX locates in the close proximity to the ryanodine receptor, then NCX would curtail Ca2 before its diffusion to global Ca2i. This will result in a global [Ca2]i decrease especially during its ascending phase rather than descending phase. Therefore, NCX would...