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Expression and characterization of the flavoprotein domain of gp91phox
Chang-Hoon Han and Mun-Han Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 3 19-26 (8 pages)
ML. Active oxygen species and the functions of phagocytic leukocytes. Annu Rev Biochem, 1980, 49, 695-726. ..PAGE:7 Expression and characterization of the flavoprotein domain of gp91phox 25 2. Biberstine-Kinkade KJ, Yu L, Dinauer MC. Mutagenesis of an arginine- and lysine-rich domain in the gp91(phox) subunit of the phagocyte NADPH-oxidase flavocytochrome b558. J Biol Chem, 1999, 274(15), 10451-7... Truncated forms of gp91(phox) were expressed in E. coli in which the N-terminal hydrophobic transmembrane region was replaced with a portion of the highly soluble bacterial protein thioredoxin (TRX). TRX-gp91(phox) (306-569), which contains the putative FAD and NADPH binding sites, showed NADPH-dependent NBT (nitroblue tetrazolium) reductase activity, whereas TRX-gp91(phox) (304-423) and TRX-gp91(phox) (424-569) were inactive. Activity saturated at about a 1:1 molar ratio of FAD to... -
Enhanced Expression of Cell Adhesion Molecules in the Aorta of Diabetic Mice is Mediated by gp91phox-derived Superoxide
MiRanYun, JongJaeKim, SunMiLee, HyeJinHeo, SunSikBae, ChiDaeKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.2 6 109-116 (8 pages)
gp91phox expression and superoxide production, and levels of blood glucose and gp91phox expression were determined by analysis of re- gression line. P ＜0.05 was considered statistically signi- ficant. RESULTS Male ob/ob mice showed a significant increase in body weight throughout the study pe riod (until 12 weeks of age) (Table 1). In addition, the plasma glucose and insulin concentrations... in control aorta (0.9⁑0.5 and 1.6⁑0.3, respectively), accompanied with the enhanced expression of gp91phox, a membrane subunit of NAD(P)H oixdase. Furthermore, there was a strong positive correlation (r=0.89, P＜0.01 in ICAM-1 and r=0.88, P＜0.01 in VCAM-1) between ICAM-1/VCAM-1 expression and vascular production of superoxide. The present data indicate that the increased production of superoxide via NAD(P)H oxidase may explain the enhanced expression of CAM in diabetic vasculatures. -
Activation domain in P67phox regulates the steady state reduction of FAD in gp91phox
Chang-Hoon Han, Mun-Han Lee 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 4 27-31 (5 pages)
..PAGE:1 . . . J. Vet. Sci. (2000), 1(1), 2731 Activation domain in P67 phox regulates the steady state reduction of FAD in gp91 phox Chang-Hoon Han and Mun-Han Lee 1* Department of Biochemistry, Swiss Federal Institute of Technology in Zurich,Universitatstrasse 16, 8092 Zurich, Switzerland 1 Department of Biochemistry, College of Veterinary Medicine, Seoul National University, Suwon 441-744,... An activation domain in p67(phox) (residues 199-210) is critical for regulating NADPH oxidase activity in cell-free system [10] To determine the steady state reduction of FAD, thioacetamide-FAD was reconstituted in gp91(phox), and the fluorescence of its oxidised form was monitored. Omission of p67(phox) decreased the steady state reduction of the FAD from 28% to 4%, but omission of p47(phox) had little effect. A series of the truncated forms of p67(phox) were expressed in E.coli to determine... -
4-O-methylhonokiol Attenuates Oxidative Stress that Triggers Teratogenesis of Embryos in Cultured Mouse Embryos Exposed to Nicotine
Lee Wha Gwon, Chunmei Lin, Jung Min Yon, Seul Gi Park, Sl Bi Chu, Beom Jun Lee, Young Won Yun, Sang Yoon Nam 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 118 199-200 (2 pages)
1 Supplement 199 induces the aggregation of lipid raft components coupled with NADPH oxidase enzymes, in which rVvhA increased the interaction of NADPH oxidase 2(NOX2, gp91phox) with a cytosolic protein NCF1 (p47phox) to facilitate the production of reactive oxygen species (ROS). rVvhA uniquely stimulated aconventional PKC isoform PKCa and induced the phosphorylation of both ERK an d JNK,... -
Lycopene Inhibits Nicotine-induced Embryo Anomalies Via Antioxidative, Anti-apoptotic, and Anti-inflammatory Activities
Seul Gi Park, Chunmei Lin, Sl Bi Chu, Jung Min Yon, Lee Wha Gwon, Beom Jun Lee, Young Won Yun, Sang Yoon Nam 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 117 199-199 (1 pages)
1 Supplement 199 induces the aggregation of lipid raft components coupled with NADPH oxidase enzymes, in which rVvhA increased the interaction of NADPH oxidase 2(NOX2, gp91phox) with a cytosolic protein NCF1 (p47phox) to facilitate the production of reactive oxygen species (ROS). rVvhA uniquely stimulated aconventional PKC isoform PKCa and induced the phosphorylation of both ERK an d JNK,... -
Vibrio vulnificus VvhA Induces Mitochondrial Cell Death Via Lipid Raft-mediated ROS Production and NF-kB Activation in Intestinal Epithelial Cells
Young Hyun Jung, Sei Jung Lee, Hyun Jik Lee, Dah Ihm Kim, Jeong Yeon Kim, Ing Ing Chai, Gee Euhn Choi, Ho Jae Han 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 116 198-199 (2 pages)
1 Supplement 199 induces the aggregation of lipid raft components coupled with NADPH oxidase enzymes, in which rVvhA increased the interaction of NADPH oxidase 2(NOX2, gp91phox) with a cytosolic protein NCF1 (p47phox) to facilitate the production of reactive oxygen species (ROS). rVvhA uniquely stimulated aconventional PKC isoform PKCa and induced the phosphorylation of both ERK an d JNK,... -
Dieckol Attenuates Microglia-mediated Neuronal Cell Death via ERK, Akt and NADPH Oxidase-mediated Pathways
YanjiCui, Jee-YunPark, JinjiWu, JiHyungLee, Yoon-SilYang, Moon-SeokKang, Sung-CherlJung, JooMinPark, Eun-SookYoo, Seong-HoKim, SangmeeAhnJ 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.3 5 219-228 (10 pages)
T. Gp91phox (NOX2) in classically activated microglia exacerbates traumatic brain injury. J Neuroinflammation. 2010;7:41. 25. Zhao S, Zhang L, Lian G, Wang X, Zhang H, Yao X, Yang J, Wu C. Sildenafil attenuates LPS-induced pro-inflammatory responses through down-regulation of intracellular ROS-related MAPK/NF-κB signaling pathways in N9 microglia. Int Immu- nopharmacol. 2011;11:468-474. 26.... 1 μg/ml)-stimulated BV-2 microglia. In addition, DEK markedly attenuated Akt phosphorylation and increased expression of gp91phox, which is the catalytic component of NADPH oxidase complex responsible for microglial reactive oxygen species (ROS) generation. Finally, DEK significantly attenuated neuronal cell death that is induced by treatment of microglial conditioned media containing neurotoxic secretary molecules. These neuroprotective effects of DEK were also confirmed in a neuron-microglia... -
Expression and Activity of the Na-K ATPase in Ischemic Injury of Primary Cultured Astrocytes
MiJungKim, JinyoungHur, In-HyeHam, HyeJinYang, YounghoonKim, SeungjoonPark, Young-WukCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.4 4 275-281 (7 pages)
Korean J Physiol Pharmacol. 2011;15:353-361. 21. Hur J, Lee P, Kim MJ, Kim Y, Cho YW. Ischemia-activated microglia induces neuronal injury via activation of gp91phox NADPH oxidase. Biochem Biophys Res Commun. 2010;391: 1526-1530. 22. Scherzer P, Popovtzer MM. Segmental localization of mRNAs encoding Na ＋-K ＋-ATPase alpha(1)-and beta(1)-subunits in diabetic rat kidneys using RT-PCR. Am J... Astrocytes are reported to have critical functions in ischemic brain injury including protective effects against ischemia-induced neuronal dysfunction. Na-K ATPase maintains ionic gradients in astrocytes and is suggested as an indicator of ischemic injury in glial cells. Here, we examined the role of the Na-K ATPase in the pathologic process of ischemic injury of primary cultured astrocytes. Chemical ischemia was induced by sodium azide and glucose deprivation. Lactate dehydrogenase assays... -
Involvement of NAD(P)H Oxidase in a Potential Link between Diabetes and Vascular Smooth Muscle Cell Proliferation
HyeYoungJeong, MiRanYun, ChiDaeKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.2 9 103-109 (7 pages)
is a multisubunit complex with 2 membrane-associated subunits (p22phox and gp91phox) and 3 cytosolic subunits (p40 phox, p47phox, and p67phox), regulated by Rac G proteins, and this oxidase has been previously identified in cu ltured VSMC and endothelial cells (Bayraktutan et al, 1998; Mohazzab et al, 1994). Although its role in signal tr ansduction is, to date, only partially understood,... The cellular mechanisms that contribute to the acceleration of atherosclerosis in diabetes are poorly understood. Therefore, the potential mechanisms involved in the diabetes-dependent increase in vascular smooth muscle cell (VSMC) proliferation was investigated. Using primary culture of VSMC from streptozotocin-induced diabetic rat aorta, cell proliferation assay showed two-fold increase in cell number accompanied with enhanced superoxide generation compared to normal VSMC, 2 days after...