자료유형
전체 선택해제
총 19건 검색
본문검색 결과만 보기
-
Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay
Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 5 143-150 (8 pages)
a result, a large volume of solvent may be required for IAC. Additional disadvantages of this technique include a potential reduction in mycotoxin exposure to the antibody and the need for a relatively long washing time. Many recent studies have reported on the application of nanoparticles in areas such as treatment for disease, drug delivery, and diagnostic techniques [5,14,19,20]. Unlike... Monoclonal antibody (mAb, NVRQS-DON) against deoxynivalenol (DON) was prepared. DON-Ag coated enzyme linked immunosorbent assay (ELISA) and DON-Ab coated ELISA were prepared by coating the DON-BSA and DON mAb. Quantitative DON calculation ranged from 50 to 4,000 ng/mL for DON-Ab coated ELISA and from 25 to 500 ng/mL for DON-Ag coated ELISA. 50% of inhibitory concentration values of DON, HT-2, 15-acetyl-DON, and nivalenol were 23.44, 22,545, 5,518 and 5,976 ng/mL based on the DON-Ab coated ELISA.... -
A novel mycotoxin purification system using magnetic nanoparticles for the recovery of aflatoxin B1 and zearalenone from feed
Hyun-Jung Kim, Sung-Hee Kim, Jin-Kyu Lee, Cheong-Up Choi, Hee-Soo Lee, Hwan-Goo Kang*, Sang-Ho Cha* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 5 363-369 (7 pages)
such as the treatment of disease, drug delivery, and diagnostics [4,9,15]. Recent studies have indicated the usefulness of nanoparticles for recovering heavy metals from water and isolating harmful microbes from livestock products [6,7,21]. The purpose of the current study was to develop an advanced purification tool for isolating mycotoxins by utilizing mAbs and magnetic nanoparticles... In this study, we developed a novel tool for purifying two mycotoxins, aflatoxin B1 (AFB1) and zearalenone (ZEN), in feed. This system utilized monoclonal antibodies (mAbs) against AFB1 and ZEN, and magnetic nanoparticles (MNPs). Among ten MNPs with different diameters and functional groups, a 100-nm diameter MNP (fMA) conjugated to an amine group (-NH2) was found to be optimum for coupling with mAbs. The optimal mAb concentrations for coupling to the fMA along with mycotoxin purification... -
The effects of green tea (Camellia sinensis) flower extract on melanin synthesis in B16-F10 melanoma cells
Chanuri-Yashara Dissanayake, Hae-Hee Moon, Kyeong-Mi Yang, Younjae Lee, Chang-Hoon Han 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2018, 제 58권 제 2호 1 65-72 (8 pages)
facilitate extraction. The solvent was removed under reduced pressure in a rotary evaporator (HS-2005V, HanShin, Korea). The extract was filtered using Whatman No.1 filter paper, and the filtrate was diluted to final concen- tration of 50 mg/mL (w/v). Cell culture and treatment B16-F10 cells were grown in monolayers, adherent to the bottom of culture flasks filled with DMEM supplemented with 10%... The present study observed the effects of a green tea (Camellia sinensis) flower extract (GTFE) on melanin synthesis in B16-F10 melanoma cells. GTFE exhibited antioxidant activity on 2,2-diphenyl-1-picrylhydrazyl and inhibited mushroom tyrosinase activity in a dose-dependent manner. Furthermore, GTFE significantly diminished α-melanocyte stimulating hormone (α-MSH) stimulated cellular melanin content and tyrosinase activity throughout the concentration range evaluated. Based on RNA sequencing... -
Analysis of antibiotic residues in milk from healthy dairy cows treated with bovine mastitis ointment using ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry
Eun-kee Park , Yong-jae Ryu , Chun-nam Cha , Chang-yeul Yoo , Suk Kim , Hu-jang Lee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2016, 제 56권 제 4호 6 233-239 (7 pages)
to protect humans from the harmful effects of anti- biotic residues in milk, the Food and Agriculture Organiza- tion (FAO) and European Union (EU) have set maximum residue levels (MRLs) of 1.5, 0.2 and 4.0 g/mL for the milk residues of NEO, STR and PEG, respectively [5, 8, 22]. In addition, Korean Ministry of Food and Drug Safety (KMFDS) has established MRLs for the residues of NEO, STR and PEG in... -
A Review on Chemical-Induced Inflammatory Bowel Disease Models in Rodents
PuneetKaurRhawa, KavinderSingh, NirmalSingh, AmteshwarSinghJaggi 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.4 1 279-288 (10 pages)
a solvent or carrier, but also aids in inducing gut inflammation by breaking the mucosal barrier [39,43]. Various researchers have demonstrated that a 12 ∼24 hr fast should precede induction of anesthesia which may be induced using ether/halothan e/chloral hydrate etc. The most common method adopted for the induction of colitis in rats involves usage of 10 mg TNBS mingled in 0.25 ml of 50%... Ulcerative colitis and Crohn’s disease are a set of chronic, idiopathic, immunological and relapsing inflammatory disorders of the gastrointestinal tract referred to as inflammatory bowel disorder (IBD). Although the etiological factors involved in the perpetuation of IBD remain uncertain, development of various animal models provides new insights to unveil the onset and the progression of IBD. Various chemical-induced colitis models are widely used on laboratory scale. Furthermore, these... -
A Novel Protein Elicitor PeBL2, from Brevibacillus laterosporus A60, Induces Systemic Resistance against Botrytis cinerea in Tobacco Plant
Ghulam Hussain Jatoi, Guo Lihua, Yang Xiufen, Muswar Ali Gadhi, Azhar Uddin Keerio, Yusuf Ali Abdulle, Dewen Qiu 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 3호 3 208-218 (11 pages)
were digested after lyophilization using 2 μl of a 20% nitrile reconstitution. 1 μl aliquot of dissolved sample was added directly to the illustration point on the target. After allowing the solvent to dry naturally, 0.5 μl of over-saturated CHCA Matrix Solution (solvent for 50% ACNO, 1% TFA) was added to the corresponding target and dried. Nitrogen was blown onto the target sample and... Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein... -
Antifungal Activity of a Synthetic Cationic Peptide against the Plant Pathogens Colletotrichum graminicola and Three Fusarium Species
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 14 316-321 (6 pages)
as four solvent control repli- cates. The plates were incubated at room temperature in the dark and the amount of growth was determined by the absorbance of each well at 550 nm with a SpectraMax 250 plate reader (Molecular Devices, Sunnyvale, CA, USA). Readings were taken daily until at least one solvent control value reached or exceeded ~0.5 absorbance units, which took 3-5 days. Single... A small cationic peptide (JH8944) was tested for activity against a number of pathogens of agricultural crops. JH8944 inhibited conidium growth in most of the tested plant pathogens with a dose of 50 μg/ml, although one isolate of Fusarium oxysporum was inhibited at 5 μg/ml of JH8944. Most conidia of Fusarium graminearum were killed within 6 hours of treatment with 50 μg/ml of JH8944. Germinating F. graminearum conidia required 238 μg/ml of JH8944 for 90% growth inhibition. The peptide did... -
Research Notes : Systemic Induction of the Small Antibacterial Compound in the Leaf Exudate During Benzothiadiazole-elicited Systemic Acquired Resistance in Pepper
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 3호 15 350-355 (6 pages)
space (apoplast) of plant tissues and cause symptoms. The apoplastic leaf exudate (LE) is believed to contain nutrients to provide food resource for phytopathogenic bacteria to survive and to bring harmful phytocompounds to protect plants against bacterial pathogens. In this study, we employed the pepper-Xanthomonas axonopodis system to assess whether apoplastic fluid from LE in pepper affects the... plant pathogens thrive in the intercellular space (apoplast) of plant tissues and cause symptoms. The apoplastic leaf exudate (LE) is believed to contain nutrients to provide food resource for phytopathogenic bacteria to survive and to bring harmful phytocompounds to protect plants against bacterial pathogens. In this study, we employed the pepper-Xanthomonas axonopodis system to assess whether apoplastic fluid from LE in pepper affects the fitness of X. axonopodis during the induction of SAR.... -
PCBs함유 폐기물 처리를 위한 플라즈마 용융기술
박현서, 송현선, 박동화, 유병수 한국열환경공학회 열환경공학 17 Pages
한국열환경공학회 열환경공학 2011, 제 8권 1호 7 51-67 (17 pages)
flying ash, since it was designated as the harmful waste, it was handled with the controlled filling up afterit was processed by the cement hardening treatm ent method. Consequently, in the case of the heavy metal leach, it was difficult to observe the influence to the PCBsing process. Table 8. Leaching testresult Classification,Testitem Leaching testresult Type PCB charging or not Sequence As <... -
Synthesis and characterization of carbon doped TiO₂ photocatalysts supported on stainless steel mesh by sol-gel method
JO. Tijani, OO. Fatoba, TC. Totito, WD. Roos, LF. Petrik 한국탄소학회 Carbon Letters 12 Pages
한국탄소학회 Carbon Letters 2017, Vol.22 6 48-59 (12 pages)
low density served as the carbon pre- cursor while DMF, an aprotic solvent, was used for the dissolu- tion of PAN which aids in the reduction of the thermal effect from the cyclization process. The mixture in a bottle was sealed and then stirred on a magnetic stirrer at room temperature (25ºC) for 12 h. After 12 h of stirring, a slightly transparent (8%) PAN/ DMF viscous solution was obtained.... This study synthesized pure anatase carbon doped TiO₂ photocatalysts supported on a stainless steel mesh using a sol-gel solution of 8% polyacrylonitrile (PAN)/dimethylformamide (DMF)/TiCl₄. The influence of the pyrolysis temperature and holding time on the morphological characteristics, particle sizes and surface area of the prepared catalyst was investigated. The prepared catalysts were characterized by several analytical methods: high resolution scanning electron microscopy (HRSEM), energy...