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Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System
Hyun Soo Kim, Kwang Soon Shin, In Wook Hwang, Chul Joong Kim, Su Mi Kim, Hae Sun Jung 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 3 13-18 (6 pages)
reaction (RT-PCR) of PRRSV RNA and cloning The RNA of PRRSV was obtained from the PRRS virus CNV-1 culture supernatant by using viral RNA extraction kit (Qiagen, U.S.A.). The cDNA of the PRRSV ORF5 gene was synthesised by reverse transription (RT) and amplified by PCR. The reverse oligonucleotide primer for ORF5 gene(5'- TTCCCGGGCTAAGGACGACCCCATTG -3') was used for priming RT reaction. The Sma I... The ORF5 gene encodes a major envelope glycoprotein (GP5), which is one of the three major proteins of porcine reproductive and respiratory syndrome virus (PRRSV). The GP5 protein has been known to be a 24.5-26 kDa N-glycosylated envelope protein. The GP5 is involved in inducing neutralizing antibodies. For this reason, the GP5 is primary candidate for the PRRSV subunit vaccine. To produce the native form of GP5 in mammalian cells, we have cloned the ORF5 gene from PRRSV CNV-1 into the Semliki... -
Semantic Key Pre-Distribution Protocol For Multi-Phase Wireless Sensor Networks
Carlos Ramos, Zita Maria Almeida do Vale 인문사회과학기술융합학회 예술인문사회융합멀티미디어논문지 12 Pages
인문사회과학기술융합학회 예술인문사회융합멀티미디어논문지 2011, 제 1권 제 1호 3 17-28 (12 pages)
task, the nature of gait indicates that it is an irreplaceable biometric [1] and can benefit the remote biometric authentication [2]. To build a successful gait recognition system, feature extraction plays a crucial role. Currently, gait feature extraction methods can be roughly divided into two major categories: model-based and model-free approaches. Model based approaches assume that the gait... to describe the spatio-temporal walking pattern for human identification by gait. The CGI temporal template encodes the temporal information among gait frames via color mapping to improve the recognition performance. Our method starts with the extraction of the contour in each gait image, followed by utilizing a color mapping function to encode each of gait contour images in the same gait sequence and compositing them to a single CGI. We also obtain the CGI-based real templates by generating CGI... -
발치지수(Extraction Index) 기준에 관한 두부 방사선학적 연구
신수정(Soo-Jung Shin), 장영일(Young-II Chang) 대한치과교정학회 The Korean Journal of Orthodontics 10 Pages
대한치과교정학회 The Korean Journal of Orthodontics 1996, 제 26권 제 4호 3 349-358 (10 pages)
is 123.6° 3. The mean distance of upper lip to E~line is 0.0 mm, lower lip to E-line is 1.4 mm, 4. The mean value of the EI is 153. 8°. KOREA. J. ORTHOD. 1996 ； 26 ： 349-358 ^ Key words ： Extraction formulas, skeletal pattern, ODI, APDI, CF, El - 358 - 신수정. 장영일 대치교정지 26권 4호, 1996년 -ABSTRACT- A Roentgenographic Study on the Extraction Index in Korean Adolescent Soo-Jung Shin,... 발치기준들(Extraction formulas)을 검토해 보면, 치열궁 장경 부조화, 치열의 전방 돌출, 그리고 환자의 골격형태에 기초하여 발치를 결정함을 알 수 있다. 이들 중에서 가장 중요하게 고려되어야 할 진단요소는 환자의 골격형태로 결국 개개 환자의 부정교합은 그 환자의 골격형태 범위 내에서 치료되어야 하기 때문이다. EI(Extraction Index)는 환자의 골격형태를 감별진단 할 수 있는 CF를 기본으로 구성되어 있고, CF는 부정교합의 수직적, 수평적 요소를 감별해내는 ODI와 APDI로 이루어져 있다. 또한 EI에는 환자의 안모에 영향을... -
무문토기시대 가구(家口) 연구
Rachel Lee, 윤호필(Ho-Pil Yun), 박용근(Yong-Geun Park) 한국고고학회 한국고고학전국대회 발표문 25 Pages
한국고고학회 한국고고학전국대회 발표문 제37회 한국고고학대회 8 162-186 (25 pages)
to extract undisturbed soil blocks from which thin sections will be created. The method of extraction varies with how well cemented the archaeological sediment is; blocks can be carved out with just a knife in clayey and silty environments but metal frames and other containers are often used when sediments are loose. 37 OK.indb 166 13. 10. 23. 오후 3:58 ..PAGE:6 Mumun Period Households: A View... -
Surveillanceof Chronic Wasting Disease in Korean Water Deer in South Korea
In Soon Roh, Hyun Joo Sohn, Hyun Jeong Kim, Tae Young Suh, Hyo Jin Kim, In Soo Cho, Jong Tak Kim, Jae Hwa Seo, Mun Seok Hur, Jip Seol Jeo 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 172 425-426 (2 pages)
administered to mice by 12 times intraperitoneal injection with dose of 100 mg/kg) and compared to normal-infected TgElk mice. Following total RNA extraction, 84 key genes in pathway for inflammatory cytokines & receptor were examin ed by real-time PCR using RT2 Profiler PCR Array. Quantitative-PCR (qPCR) was performed on an ABI Prism 7500 Sequence Detection System (Applied Biosystems) in a... -
Gene Expression Alterations between a Persistently PrPBSE Infected and Rubus coreanus Miquel - cured M2B Cell Line
Hyo Jin Kim, Hyun Jeong Kim, In Soon Roh, Won Yong Lee, Tae Young Suh, Hoo Chang Park, Byoung han Kim, Hyun Joo Sohn 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 138 404-405 (2 pages)
expression was examined in BSE-infected M2B cell line and compared to RCM-cured M2B cell line. Following total RNA extraction, we utilized gene sp ecific microarray chip assays and real-time PCR experiment to analyze gene expression and determine pathway activity, respectively. Real-time PCR quantification was performed on an ABI Prism 7500 Sequence Detection System (Applied Biosystems) in... -
Results of the Monitoring of Veterinary Drug and Chemical Residues in Domestic Meats in 2014
Hyo Jin Kim, Soo hee Kim, Byung Jae So, Jae Young Song, Sung Won Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 68 173-174 (2 pages)
detection of multiple bee viruses. Materials and Methods: Bee samples used for virus screening were collected from all over the country. Viral RNA was extracted using viral gene-spin viral DNA/RNA extraction kit(Intron). Specific primer for SBV, BQCV, KBV, DWV, IAPV and ABPV were designed based on the published nucleotide sequences. To screening for virus infection in honeybee colonies, bro... -
Spatiotemporal Distribution of Migratory Bird Species with Potential Transmission of Highly Pathogenic Avian Influenza
Son Il Pak, Woo-seog Jeong, Hong Shik Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 63 216-217 (2 pages)
time of data extraction with a final diagnosis HPAI were available for analysis. Data were constructed in Microsoft Excel, and epidemiological analyses were performed on descriptive data, using the SAS software version 9.4. Geocoding and mapping was accomplished with ArcGIS (ESRI). Kernel density estimation (KDE) was used to identify risk regions. All data were mapped using the latitude and...