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Development of Rapid Immune-gold Strip Kit for On-Site Diagnosis ofTomato spotted wilt virus
한국식물병리학회 식물병연구 2014, 20권 1호 3 15-20 (6 pages)
to Tomato bush stunt virus, Cucumber mosaic virus and Tomato yellow leaf curl virus that cause severe damage to tomato pro- duction. These results confirm the high specificity and reliability of the RIGS for TSWV detection in tomato and other crops (Fig. 5). The stability of the RIGS strip assay was evaluated by com- paring the analysis of TSWV-infected crude sap before and after the strip... -
Effect of Cyclic Nucleotides on Phorbol Ester-Induced Contraction in Rabbit Carotid Artery
Jung, Dong-Keun, Woo, Jae-Suk, Jung, Jin-Sup, Kim, Yong-Keun, Lee, Sang-Ho 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 1호 4 39-50 (12 pages)
uptake in depolarized rabbit carotid artery strip but not in control (Fig. 4). This means that PDBu increases Ca 2 + flux via the inactivated voltage dependent Ca 2 + channel without any change in membrane permeability. Without concomitant change in membrane poten- tial, PDBu does not increase intracellular Ca 2 concentration. Isoproterenol, a (3 -adrenergic agonist, is known to activate... This study was designed to clarify the action of cyclic nucleotides, cyclic AMP and cyclic GMP, on phorbol 12,13-dibutyrate (PDBu)-induced contraction in rings isolated from rabbit carotid artery. Arterial rings, 2 mm in width, were myographied isometrically in an isolated organ bath. PDBu produced slowly developing, sustained contraction in rabbit carotid artery, in a dose dependent manner, which was independent of extracellular Ca2+ PDBu-induced contraction was relaxed by staurosporine, which... -
Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips
Na-Kyeong Kim, Hyo-Jeong Lee, Sang-Min Kim, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 8 159-166 (8 pages)
for the detection of BYDV. (A) Principle of the recombinase polymerase am- plification. (B) Schematic diagram of the RPA-LFS. RPA-LFS, recombinase polymerase amplification combined with lateral flow strip; BYDV, barley yellow dwarf virus; FAM, 6-carboxyfluoreescein; THF, tetrahydrofuran. ..PAGE:3 Rapid Detection of BYDV Using RPA-LFS 161 terminus, a tetradrofuran (THF) spacer at the center, and... Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat... -
Synergistic Interactions of Schizostatin Identified from Schizophyllum commune with Demethylation Inhibitor Fungicides
Min Young Park, Byeong Jun Jeon, Ji Eun Kang, Beom Seok Kim 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 5 579-590 (12 pages)
g/ml tebuconazole, schizostatin (1 g/ml) showed disease control efficacy against gray mold on tomato leaf similar to that achieved with 20 g/ml te- buconazole treatment alone. Interestingly, schizostatin showed demethylation inhibitor (DMI)-specific syn- ergistic interactions in the crossed-paper strip assay using commercial fungicides. In a checkerboard assay with schizostatin and DMIs, the... with 5 μg/ml tebuconazole, schizostatin (1 μg/ml) showed disease control efficacy against gray mold on tomato leaf similar to that achieved with 20 μg/ml tebuconazole treatment alone. Interestingly, schizostatin showed demethylation inhibitor (DMI)-specific synergistic interactions in the crossed-paper strip assay using commercial fungicides. In a checkerboard assay with schizostatin and DMIs, the fractional inhibitory concentration values were 0.0938-0.375. To assess the molecular mechanisms... -
Rapid Detection of Lily mottle virus and Arabis mosaic virus Infecting Lily (Lilium spp.) Using Reverse Transcription Loop-Mediated Isothermal Amplification
Yubao Zhang, Yajun Wang, Zhongkui Xie, Ruoyu Wang, Zhihong Guo, Yuhui He 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 2호 6 170-178 (9 pages)
an immunochrographic strip test for rapid detection of Lily symptomless virus. J. Virol. Methods 220:13-17. Zhang, Y., Wang, Y., Xie, Z., Yang, G., Guo, Z. and Wang, L. 2017. Simultaneous detection of three lily viruses using Triplex IC-RT-PCR. J. Virol. Methods 249:69-75. Zhang, Y., Wang, Y., Xie, Z., Yang, G., Guo, Z. and Wang, L. 2018. The occurrence and distribution of viruses infecting... The Lily mottle virus (LMoV) impedes the growth and quality of lily crops in Lanzhou, China. Recently Arabis mosaic virus (ArMV) has been detected in LMoV- infected plants in this region, causing plant stunting as well as severe foliar symptoms, and likely posing a threat to lily production. Consequently, there is a need to develop simple, sensitive, and reliable detection methods for these two viruses to prevent them from spreading. Reverse transcription (RT) loop-mediated isothermal... -
Development of an Improved Loop-Mediated Isothermal Amplification Assay for On-Site Diagnosis of Fire Blight in Apple and Pear
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 3호 5 191-198 (8 pages)
al., 2011) and the strip was commercialized (Ea AgriStrip, BIOREBA AG, Reinach, Switzerland). Although it seemed to be useful for on-site diagnosis of fire blight, it and the similar strip could not distinguish E. amylovora from E. pyrifoliae nor diagnose fire blight separately from black shoot blight caused by E. pyrifoliae (Heo et al., 2017). Loop-mediated isothermal amplification (LAMP)... Fast and accurate diagnosis is needed to eradicate and manage economically important and invasive diseases like fire blight. Loop-mediated isothermal amplification (LAMP) is known as the best on-site diagnostic, because it is fast, highly specific to a target, and less sensitive to inhibitors in samples. In this study, LAMP assay that gives more consistent results for on-site diagnosis of fire blight than the previous developed LAMP assays was developed. Primers for new LAMP assay (named as... -
Efficient Transmission and Propagation of Tomato Chlorosis Virus by Simple Single-Leaflet Grafting
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 13 345-349 (5 pages)
to- 346 Fig. 1. Grafting of single leafets to tomato seedlings. (A) Six-week-old tomato seedlings (cv. Tenten) were used as recipient plants for single-leafet grafting inoculation. (B) The frst leaf was removed from the stem of the recipient seedling to create an incision for grafting. (C) Leafets were taken from tomato chlorosis virus- or tomato yellow leaf curl virus-infected tomato plants.... Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient... -
Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 1호 9 80-86 (7 pages)
a strip of ny- lon membrane (40 × 10 mm), Biodyne plus (Pall, Tokyo, Japan), by high-throughput liquid handling systems with glass syringes (Shieh et al., 2002). To prepare such a mac- roarray, we can either ask a supplier (e.g., Hokusan, Hok- kaido, Japan) to make a macroarray or prepare a hand- made macroarray by ourselves; there are commercially available kits to make a hand-made array such... One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract... -
Reverse Transcription Droplet Digital PCR을 활용한 Tomato Spotted Wilt Virus 검출 및 정량
이효정, 박기범, 한연수, 정래동 한국식물병리학회 식물병연구 8 Pages
한국식물병리학회 식물병연구 2021, 27권 3호 5 120-127 (8 pages)
스트립 기반 역전사 재조합효소-중합효소 증폭법(reverse-transcription recombinase polymerase amplification assay combined with lateral flow strip) 등이 개발되었다(Cho 등, 1988; Debreczeni 등, 2011; Fukuta 등, 2004; Karavina and Gubba, 2017; Lee 등, 2021b; Roberts 등, 2000). 다양한 기법들 중에서 TSWV를 진단하기 위한 가장 보편적인 방법은 1세대 PCR인 전기 영동 기반 일반 PCR 기 법인데, 일반 PCR의... 식물 바이러스는 작물 수확량에 상당한 손실을 일으키고 작 물 생산을 지속적으로 위협하여 세계 식량 안보에 심각한 위협 이 된다. 그 중 tomato spotted wilt virus (TSWV)는 주로 원예 작물을 감염시키는 가장 위협적인 식물 바이러스로 넓은 기주 범위를 가진다. Reverse-transcription quantitative real-time PCR (RT-qPCR)은 TSWV의 민감한 검출을 위해 널리 사용되 고 있지만 표준화의 어려움으로 인해 유용성이 감소한다. 따라 서 본 연구에서는 TSWV 검출을 위해 민감하고 정확한 reverse transcription droplet digital... -
항혈청 기반 진단 스트립을 이용한 과수 화상병 현장진단
허광일, 신두산, 손수형, 오창식, 박덕환, 이영기, 차재순, 한국식물병리학회 식물병연구 8 Pages
한국식물병리학회 식물병연구 2017, 23권 4호 2 306-313 (8 pages)
결 과 스트립의 국내 분리 Ea와 Ep에 대한 특이성 및 검출민감 도. Ea AgriStrip과 EB strip의 국내에서 분리된 화상병균(Ea), 가지검은마름병균(Ep), 그리고 사과나무 가지마름병균(Pss)에 Fig. 1. Fire blight symptoms used for strip assays. Bacterial ooze (1), shoot blight (2), stem canker (3), fruitlet (4), leaf and leafstalk (5), trunk canker (6). ..PAGE:5 Research in Plant Disease Vol. 23 No. 4 310...