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Microbial Community Dysbiosis and Functional Gene Content Changes in Apple Flowers due to Fire Blight
Hyun Gi Kong, Hyeonheui Ham, Mi-Hyun Lee, Dong Suk Park, Yong Hwan Lee 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 4호 10 404-412 (9 pages)
pathways showed that pathways related to syringate, glucose, xylose degradation, and thiamin diphos- phate biosynthesis were specifically enriched in diseased samples, whereas those related to glycogen degradation were specifically enriched in healthy samples (Fig. 5). In addition, pathways related to RR-butanediol, 2,3-butane- diol, gallate, and catechol degradation were more highly enriched... a unique microbial community at the species level. Pantoea agglomerans and P. allii were the most predominant bacteria in healthy flowers, whereas E. amylovora comprised more than 90% of the microbial population in diseased flowers. Furthermore, gene function analysis revealed that glucose and xylose metabolism were enriched in diseased flowers. Overall, our results showed that the microbiome of apple blossoms is rich in specific bacteria, and the nutritional composition of flowers is important... -
Sampling and Selection Factors that Enhance the Diversity of Microbial Collections: Application to Biopesticide Development
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 2호 4 144-153 (10 pages)
of antibiotic biosynthesis genes (McSpadden Gardener et al., 2000). And, phylogenetic markers have been used to identify and recover novel and active biocontrol bacteria when used in combination with microbial community profiling (Benitez and McSpadden Gardener, 2009). However, to our knowledge, this is the first report of using marker-assisted selection to improve the efficiency of traditional... Diverse bacteria are known to colonize plants. However, only a small fraction of that diversity has been evaluated for their biopesticide potential. To date, the criteria for sampling and selection in such bioprospecting endeavors have not been systematically evaluated in terms of the relative amount of diversity they provide for analysis. The present study aimed to enhance the success of bioprospecting efforts by increasing the diversity while removing the genotypic redundancy often present in... -
A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer
Qing Wang, Mianmian Chen, Wei Zhang 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 12 260-270 (11 pages)
3 Penicillin and cephalosporin biosynthesis 3 (0.96%) 3 (0.3%) ko00311 4 Sulfur metabolism 4 (1.28%) 5 (0.51%) ko00920 5 RNA degradation 5 (1.6%) 8 (0.81%) ko03018 6 Selenocompound metabolism 5 (1.6%) 9 (0.91%) ko00450 esis 3 (0.96%) 5 (0.51%) ko00770 8 Cysteine and methionine metabolism 7 (2.24%) 16 (1.62%) ko00270 9 Cyanoamino acid metabolism 2 (0.64%) 3 (0.3%) ko00460 10 Ubiquinone and other... Similar to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682 upregulated genes were identified in vivo. Among the upregulated genes, a signal transduction... -
In silico analysis of putative drug and vaccine targets of the metabolic pathways of Actinobacillus pleuropneumoniae using a subtractive/comparative genomics approach
Biruk T. Birhanu, Seung-Jin Lee, Na-Hye Park, Ju-Beom Song, Seung-Chun Park 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 5 188-199 (12 pages)
Biosynthesis of secondary metabolites apl01110 22 Microbial metabolism in diverse environments apl01120 The unique pathways were identified from the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database of the Apleuropneumoniae L20 (serotype 5b) strain, which has 104 metabolic pathways. The pathways in this strain were compared with the 295 and 299 metabolic pathways of pig and human,... Actinobacillus pleuropneumoniae is a Gram-negative bacterium that resides in the respiratory tract of pigs and causes porcine respiratory disease complex, which leads to significant losses in the pig industry worldwide. The incidence of drug resistance in this bacterium is increasing; thus, identifying new protein/gene targets for drug and vaccine development is critical. In this study, we used an in silico approach, utilizing several databases including the Kyoto Encyclopedia of Genes and... -
All blood, No stool: enterohemorrhagic Escherichia coli O157:H7 infection
Jang W. Yoon, Carolyn J. Hovde 대한수의학회 Journal of Veterinary Science 14 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 1 219-232 (14 pages)
A biosynthesis in Escherichia coli O157:H7. Infect Immun 2004, 72, 1174-1180. 69. Klapproth JM, Scaletsky IC, McNamara BP, Lai LC, Malstrom C, James SP, Donnenberg MS. A large toxin from pathogenic Escherichia coli strains that inhibits lymphocyte activation. Infect Immun 2000, 68, 2148-2155. 70. Knutton S, Rosenshine I, Pallen MJ, Nisan I, Neves BC, Bain C, Wolff C, Dougan G, Frankel G. A... Enterohemorrhagic Escherichia coli serotype O157:H7 is a pathotype of diarrheagenic E. coli that produces one or more Shiga toxins, forms a characteristic histopathology described as attaching and effacing lesions, and possesses the large virulence plasmid pO157. The bacterium is recognized worldwide, especially in developed countries, as an emerging food-borne bacterial pathogen, which causes disease in humans and in some animals. Healthy cattle are the principal and natural reservoir of E.... -
Tactics of Mycobacterium avium subsp. paratuberculosis for intracellular survival in mononuclear phagocytes
Seng-Ryong Woo, Charles J. Czuprynski* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 1 1-8 (8 pages)
Complement biosynthesis by mononuclear phagocytes. Immunol Res 1993, 12, 213-232. 63. Medzhitov R, Preston-Hurlburt P, Janeway CA Jr. A hu- man homologue of the Drosophila Toll protein signals acti- vation of adaptive immunity. Nature 1997, 388, 394-397. 64. Mellman I, Fuchs R, Helenius A. Acidification of the en- docytic and exocytic pathways. Annu Rev Biochem 1986, 55, 663-700. 65. Miller BH,... Johne's disease is a condition that refers to chronic granulomatous enteritis in ruminants. It is believed that survival and replication of Mycobacterium (M.) paratuberculosis in mononuclear phagocytes plays an important role in the pathogenesis of Johne's disease. However, it is not clear how M. paratuberculosis survives for long time periods in mononuclear phagocytes, nor is it clear which factors trigger multiplication of these bacilli and result in the development of Johne's disease.... -
Isolation of cholesterol-lowering lactic acid bacteria from human intestine for probiotic use
Hyeong-Jun Lim, So-Young Kim, Wan-Kyu Lee 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 16 391-395 (5 pages)
by the biosynthesis of cholesterol from saturated fat, removal of cholesterol from the circulation, absorption of dietary cholesterol, and excretion of cholesterol via bile and feces. Cellular cholesterol homeostasis is very important for the prevention of cardiovascular disease, and numerous studies have been already reported that enzyme inhibitors for 3- hydroxy-3-methylglutaryl coenzyme A... Cholesterol-lowering effect of lactic acid bacteria (LAB: Streptococcus, Lactobacillus and Bifidobacterium) is well-known. Thus, we investigated LAB isolated from human intestine on the cholesterol-lowering effect in vitro. Seven Streptococcus (61.1%), 11 Lactobacillus (71.8%) and 7 Bifidobacterium (27.9%) were isolated as acid (pH 2.5 and 3.0) and bile (0.3% oxgall) tolerant strains. Streptococcus HJS-1, Lactobacillus HJL-37 and Bifidobacterium HJB-4 were finally selected as probiotic strains... -
Application of biotechnological tools for coccidia vaccine development
Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 1 279-288 (10 pages)
Avian Dis 1998, 42, 397-403. 4.Almasy L, Blanqero J. Multipoint quantitative-trait linkage in general pedigrees. Am J Human Genet 1998, 62, 1198- 1211. 5.Anderson ME. Glutathione: an overview of biosynthesis and modulation. Chem Biol Interact 1998, 24, 1-14. 6.Arnaud-Battandier F, Lawrence EC, Blaese RM. Lymphoid populations of gut mucosa in chickens. Digest Dis Sci 1980, 25, 252-59. 7.Assreuy J,... Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of host-parasite interactions and... -
N-acetyl-L-cysteine and cysteine increase intracellular calcium concentration in human neutrophils
Md.AshrafulHasan, Won-GyunAhn, Dong-KeunSong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.5 2 449-457 (9 pages)
(H20150551) and Hallym University Research Fund (HRF- 201602-015). CONFLICTS OF INTEREST The authors declare no conflicts of interest. REFERENCES 1. Murray RK, Granner DK, Mayes PA, Rodweil VW. Biosynthesis of nutritionally nonessential amino acids. Harper’s Biochemistry. 22nd ed. USA: Appleton & Lange; 1991. p.267-269. 2. Kelly GS. Clinical applications of N-acetylcysteine. Altern Med Rev.... N-acetyl-L-cysteine (NAC) and cysteine have been implicated in a number of human neutrophils’ functional responses. However, though Ca2+ signaling is one of the key signalings contributing to the functional responses of human neutrophils, effects of NAC and cysteine on intracellular calcium concentration ([Ca2+]?) in human neutrophils have not been investigated yet. Thus, this study was carried out with an objective to investigate the effects of NAC and cysteine on [Ca2+]? in human neutrophils.... -
Cytotoxicological Effect of Tebufenozide, an Insect Growth Regulator (IGR): Stimulation of Filamentous Actin Reorganization and Enhancement of Hsp27 Expression in Drosophila Kc Cells
JeeNaHwang, Hwa-JinJung, YoungRokSeo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.6 10 351-354 (4 pages)
moulting hormone-responsive genes from Drosophila. Insect Biochem 16: 241 －248, 1986 Cherbas L, Fristrom JW and O'connor JD. Biosynthesis, Metab- olism and Mode of Action of Invertebrate Hormones. In: Hoffmann J, Porchet M ed, Proceedings in Life Sciences. Springer- Verlag, Berlin, p 305-322, 1984 Cherbas P, Cherbas L, Williams CM. Induction of acetylcho- linesterase activity by -ecdysone in a... The cytotoxicological responses to insect growth regulator (IGR), using tebufenozide as ecdysteroid mimic, were investigated in Drosophila Kc cells. Treatment of Kc cells with tebufenozide showed significant growth inhibition and striking morphological changes including aggregation and elongation of the cells. In order to understand the cellular mechanism underlying the response of Drosophila cells to tebufenozide, immunofluorescence microscopy was performed. We found that treatment of Kc cells...