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Development of a universal plate-agglutination test for detecting Haemophilus parasuis
Dingqian Guo, Cheng Tang, Quan Hai, Guoqing Shao, Hua Yue* 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 13 355-357 (3 pages)
C. Haemophilus parasuis: new trends on diagnosis, epidemiology and control. Vet Microbiol 2004, 99, 1-12. 11. Olvera A, Cerda-Cuellar M, Aragon V. Study of the population structure of Haemophilus parasuis by multilocus sequence typing. Microbiology 2006, 152, 3683-3690. 12. Olvera A, Segales J, Aragon V. Update on the diagnosis of Haemophilus parasuis infection in pigs and novel genotyping... for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical isolates. Further, the specificity of the method was validated with 22 bacterial strains from 12 related species. -
Antifungal and Anticancer Activities of a Protein from the Mushroom Cordyceps militaris
ByungTaePark, KwangHeumNa, EuiChaJung, JaeWanPark, HaHyungKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.1 8 49-54 (6 pages)
dimer whose subunits interact through noncovalent bonds. Even though the partial amino acid sequence and secondary structure of CMP are quite different from those of trypsin, CMP appears to be a trypsin-like serine protease. However, unlike trypsin, CMP has multiple func- tions not only as a protease bu t also in exerting antifungal effect and cytotoxicity against human cancer cells. Our re-... protease inhibitor phenylmethylsulfonyl fluoride. Amino acid composition of intact CMP and amino acid sequences of three major peptides from a tryptic digest of CMP were determined. CMP exerted strong antifungal effect against the growth of the fungus Fusarium oxysporum, and exhibited cytotoxicity against human breast and bladder cancer cells. These results indicate that C. militaris represents a source of a novel protein that might be applied in diverse biological and medicinal applications. -
Field Sanitation and Foliar Application of Streptomyces padanus PMS-702 for the Control of Rice Sheath Blight
Chia-Jung Yang, Tzu-Pi Huang, Jenn-Wen Huang 한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 1호 5 57-71 (15 pages)
by S. padanus PMS-702, experiments were conducted to determine the effect of fungichromin on the mycelial viability of R. solani RS1-731, the electro- lyte leakage from hyphae of R. solani RS1-731, and the infection structure formation of R. solani RS1-731 on rice seedlings. Assays for the viability of R. solani using a Live/ Dead BacLight Bacterial Viability Kit revealed that the mycelia of ... Rice sheath blight (ShB), caused by Rhizoctonia solani Kühn AG1-IA, is one of the destructive rice diseases worldwide. The aims of this study were to develop bio- control strategies focusing on field sanitation and foliar application with a biocontrol agent for ShB manage- ment. Streptomyces padanus PMS-702 showed a great antagonistic activity against R. solani. Fungichromin produced by S. padanus PMS-702, at 3.07 mg/l inhib- ited 50% mycelial growth, caused leakage of cytoplasm, and... -
Isolation and Characterization of a Bacteriophage Preying an Antifungal Bacterium
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 6호 13 584-588 (5 pages)
(gram-) positive perspective. FEMS Microbiol. Lett. 171:1-9. Erill, I. and Caruso, S. M. 2015. Complete genome sequence of Bacillus cereus group phage tsarbomba. Genome Announc. 3: e01178-15. Fujiu, M., Sawairi, S., Shimada, H., Takaya, H., Aoki, Y., Oku- da, T. and Yokose, K. 1994. Azoxybacilin, a novel antifungal agent produced by Bacillus cereus NR2991. Production, iso- lation and structure... Several Bacillus species were isolated from rice field soils, and 16S rRNA gene sequence analysis showed that Bacillus cereus was the most abundant. A strain named BC1 showed antifungal activity against Rhizoctonia solani. Bacteriophages infecting strain BC1 were isolated from the same soil sample. The isolated phage PK16 had an icosahedral head of 100 ± 5 nm and tail of 200 ± 5 nm, indicating that it belonged to the family Myoviridae. Analysis of the complete linear dsDNA... -
Experimental study on synthesis of Co/CeO₂-doped carbon nanofibers and its performance in supercapacitors
Jongwan Kim, Zafar Khan Ghouri, Rabia Zafar Khan, Taehee An, Mira Park, Hak-Yong Kim 한국탄소학회 Carbon Letters 5 Pages
한국탄소학회 Carbon Letters 2015, Vol.16 No.4 8 270-274 (5 pages)
of NiO with ordered mesoporous structure synthesized by tem- plate SBA-15. Electrochim Acta, 51, 3223 (2006). http://dx.doi. org/10.1016/j.electacta.2005.09.013. [21] Prasad KR, Miura N. Electrochemically synthesized MnO2-based mixed oxides for high performance redox supercapacitors. Elec- trochem Commun, 6, 1004 (2004). http://dx.doi.org/10.1016/j.ele- com.2004.07.017. [22] Toupin M, Brousse T,... -
Preparation and pH-Sensitive Release Behavior of Alginate/Activated Carbon Composite Magnetic Hydrogels
Min-Hee Han, Jumi Yun, Young-Seak Lee, Hyung-Il Kim 한국탄소학회 Carbon Letters 5 Pages
한국탄소학회 Carbon Letters 2010, Vol.11 No.2 8 122-126 (5 pages)
to the intestine [16-17]. Activated carbon (AC) is one of the most effective adsorbents for several organic compounds because of their extended surface area, high adsorption capacity, micro porous structure, and specific surface activity [18-19]. Coconut shell-based AC is an effective candidate for drug adsorbent because it has high specific surface area and well-developed pore structure. Vitamin... -
Studies on Nanostructures Amorphous Carbon by X-ray Diffraction and Small Angle X-ray Scattering
K.Dasgupta, P.S.R.Krishna, R.Chitra, D.Sathiyamoorthy 한국탄소학회 Carbon Letters 4 Pages
한국탄소학회 Carbon Letters 2003, Vol.4 No.1 2 10-13 (4 pages)
understand the mesoscopic structure of this sample, we used the SAXS data. The diffraction data have been analyzed using the well known RAD program [11] to get the structure factor and the radial distribution function, (RDF). These samples were also characterized by optical micros- copy. Polarized light with cross polarizers and lambda plate was used to ensure their amorphous and isotropic nature... -
Effect of Bevacizumab on Human Tenon’s Fibroblasts Cultured from Primary and Recurrent Pterygium
YoungMinPark, ChiDaeKim, JongSooLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.4 8 357-363 (7 pages)
influence on inhibition of cellular proliferation and subsequent synthesis of type I collagen and laminin in primary and recurrent pterygia. Ophthalmic Res. 2001;33:140-146. 28. Nimmi ME . Fibrillar collagens: their biosynthesis, molecular structure, and mode of assembly. In: Zern MA, Reid LM, editors. Extracellular matrix. New York, NY: Marcel Decker; 1993. p.121-148. 29. Risteli L, Risteli... in MMP-1, PIP, and laminin levels. Distinctly, the inhibitory effect of bevacizumab on MMP-1 level related with collagenase in primary pterygium HTFs was significantly higher than that of recurrent pterygium. Significant changes in cellular density and morphology both occurred at bevacizumab concentrations greater than 7.5 mg/mL. Only primary pterygium HTFs had a reduction in cellular density at a bevacizumab concentration of 5.0 mg/mL. Bevacizumab inhibits primary and recurrent pterygium HTFs ... -
Identification, Characterization, and Efficacy Evaluation of Bacillus velezensis for Shot-Hole Disease Biocontrol in Flowering Cherry
Viet-Cuong Han, Nan Hee Yu, Hyeokjun Yoon, Neung-Ho Ahn, Youn Kyoung Son, Byoung-Hee Lee, Jin-Cheol Kim 한국식물병리학회 The Plant Pathology Journal 16 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 5 115-130 (16 pages)
H., Niu, Y., Huo, R. and Gao, X. 2017. Bacillus volatiles adversely afect the physiology and ultra-structure of Ralstonia solanacearum and induce sys- temic resistance in tobacco against bacterial wilt. Sci. Rep. 7:40481. Wang, S. L. and Chang, W.-T. 1997. Purifcation and character- ization of two bifunctional chitinases/lysozymes extracel- lularly produced by Pseudomonas aeruginosa K-187 in a... CFCFs led to a large reduction in the severity of the leaf spots caused by Epicoccum tobaicum and Bukholderia contaminans, respectively. In addition, the two antagonists produced indole-3-acetic acid, siderophore, and a series of hydrolytic enzymes, along with the formation of a substantial biofilm. To our knowledge, this is the first report of the antimicrobial activities of the diffusible compounds and VOCs of B. velezensis against the SH pathogens and their efficiency in the biocontrol of SH.