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01.

Comparision of three different commercial ELISAs for detection of specific antibodies to Porcine Reproductive and Respiratory Syndrome Virus in porcine sera

Hyeon-jeong Kang, Ji Eun Yu, In Ohk Ouh, Seung min Song, In Soo Cho, Sang Ho Cha 대한수의학회 대한수의학회 학술대회발표집 2 Pages

대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 59 115-116 (2 pages)

0.4 (cut-off value) were considered positive for PRRSV-specific antibodies. IFA was conducted with same serum samples as a reference standard. Results: Using IFA as a reference standard, the sensitivities of three commercial ELISA (IDEXX, MEDIAN, and Bionote) were 95.9%, 93.5%, and 91.0%, respectively. And the specificities of three commercial ELISA (IDEXX, MEDIAN, and Bionote) were 93.6%,...
- 무료
02.

Establishment of standard temperature and clinical application using thermography in horse

Su Min Kim, Gil Jae Cho 대한수의학회 대한수의학회 학술대회발표집 1 Pages

대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 136 284-284 (1 pages)

to establish standard body temperature and compare thermal differences with diseased tissues of horses. In specific, We compared thermography and radiology results of horses having hoof abscess and confirmed thermography was effective in this case. Furthermore, We isolated some bacteria from hoof and conducted antimicrobial sensitivity test for curing hoof abscess effectively. Materials and...
- 무료
03.

Hepatitis Evirus in specific pathogen free rabbits

Sang Hoon Han, Byung Joo Park, Hee Seop Ahn, Yong Hyun Kim, Joong Bok Lee, Seung Yong Park, Chang Seon Song, Sang Won Lee, In Soo Choi 대한수의학회 대한수의학회 학술대회발표집 2 Pages

대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 133 282-283 (2 pages)

± standard deviation. Statistical analysis among groups were assessed by two-way analysis of variance (ANOVA) with Dunnett's t-tests. p<0.05 was considered to indicate a statistically significant. Results: The anti-microfilarial activity of Juglans nigra green hull extract was superior in Hexan-fraction and Chloroform-fraction. Dose-dependent results were observed, but the other extracts...
- 무료
04.

Establishment of standard temperature and clinical application using thermography in horse

Su-Min Kim, Gil-Jae Cho 대한수의학회 대한수의학회 학술대회발표집 1 Pages

대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 45 224-224 (1 pages)

to establish standard body temperature and compare thermal differences with diseased tissues of horses. In specific, We compared thermography and radiology results of horses having hoof abscess and confirmed thermography was effective in this case. Furthermore, We isolated some bacteria from hoof and conducted antimicrobial sensitivity test for curing hoof abscess effectively. Materials and...
- 무료
05.

Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine

Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 11 99-109 (11 pages)

at room temperature to separate the serum, to identify the presence of specific anti-PRRSV IgG antibodies by ELISA using a PRRSV Antibody (PRRS-Ab) Test Kit (LSI, France) according to the manufacturer’s protocol. This ELISA uses membrane proteins (M; GP2, GP3, GP4, and GP5) of PRRSV as the major envelope antigen, and can therefore detect GP5-specific antibodies. Neutralization assay 　Serum...
- 4,000원
percentages of CD4 and CD8 T lymphocytes, proliferation indices, and interferon-γ expression were analyzed. Results revealed that the piglets co-immunized with pcDNA-ORF5 + pcDNA-IL-4 + pcDNA-IL-2 plasmids developed significantly higher antibody titers and neutralizing antibody levels, had significantly increased levels of specific T lymphocyte proliferation, elevated percentages of CD4 and CD8 T lymphocytes, and significantly higher IFN-γ production than the other inoculated pigs (p ＜ 0.05).
06.

The critical time of avian leukosis virus subgroup J-mediated immunosuppression during early stage infection in specific pathogen-free chickens

Feng Wang, Xiaowei Wang, Hongbo Chen, Jianzhu Liu, Ziqiang Cheng* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 6 235-241 (7 pages)

r o u p J (ALV-J)-mediated immunosuppression was determined by body weight, relative immune organ weight, histopathology, and presence of group specific antigen and antibodies in specific pathogen-free (SPF) chickens. CD4 + and CD8 + cell activity in the spleen, total and differential leukocyte counts in blood, and viral RNA levels in spleen were measured. Significant growth suppression was...
- 3,000원
J (ALV-J)-mediated immunosuppression was determined by body weight, relative immune organ weight, histopathology, and presence of group specific antigen and antibodies in specific pathogen-free (SPF) chickens. CD4 and CD8 cell activity in the spleen, total and differential leukocyte counts in blood, and viral RNA levels in spleen were measured. Significant growth suppression was observed in the two ALV-J-infected groups. A strong immune response by infected groups was present in spleen at...
07.

Establishment of a standard operating procedure for predicting the time of calving in cattle

Dominik Streyl, Carola Sauter-Louis, Anna Braunert, Dorothee Lange, Frank Weber, Holm Zerbe 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 11 177-186 (10 pages)

predictive tool during the prepartal period. We compared this semi-quantitative PRBT with the standard quantitative progesterone EIA analysis. The PRBT had a sensitivity of 90.2% and a specificity of 74.9% for detecting progesterone levels higher or lower than 1.2 ng/mL (Fig. 3). Partus prediction by using a combination of the PS and PRBT in cows: PS-PRBT We developed an SOP that combines the...
- 3,000원
By combining the PS and PRBT (if PS ≥ 4), the prediction of calving within the next 12 h improved from 14.9% to 53.1%, and the probability of ruling out calving was 96.8%. The PRBT was compared to the results of an enzyme immunoassay (sensitivity, 90.2%; specificity, 74.9%). The standard operating procedure developed in this study that combines the PS and PRBT will enable veterinarians to rule out or predict calving within a 12 h period in cows with high accuracy under field conditions.
08.

Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl3-induced toxicity in the rat forebrain cortex

Ivana D. Stevanović*, Marina D. Jovanović, Ankica Jelenković, Miodrag Čolić, Ivana Stojanović, Mil 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 3 15-22 (8 pages)

stress Introduction Neurodegenerative changes are morphologically characterized by progressive cell loss in vulnerable neuronal populations of the central nervous system (CNS), including specific regions of the hippocampus and specific subcortical regions. These changes are often associated with cytoskeletal protein aggregates that form intracytoplasmic and/or intranuclear inclusions in...
- 3,000원
The present experiments were done to determine the effectiveness of a non-specific nitric oxide synthase inhibitor, N-nitro-L-arginine methyl ester (L-NAME), on oxidative stress parameters induced by aluminium chloride (AlCl3) intrahippocampal injections in Wistar rats. Animals were sacrificed 3 h and 30 d after treatments, heads were immediately frozen in liquid nitrogen and forebrain cortices were removed. Crude mitochondrial fraction preparations of forebrain cortices were used for the...
09.

Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules

William C. Davis*, Mary Jo Hamilton 대한수의학회 Journal of Veterinary Science 16 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 8 51-66 (16 pages)

3 cycles of washing, the lymphocytes were incubated with second step reagents. For most of the studies, combinations of mAbs of different isotype were used with isotype specific goat anti-mouse immunoglobulins conjugated with fluorescein (FL), phycoery- thrin (PE), PE-Cy5, or Cy5 (Caltag Laboratories, USA). Where the mAbs of interest were the same isotype, Zenon Fab fragments of goat isotype...
- 4,500원
Flow cytometry was used to identify and characterize monoclonal antibodies (mAbs) that react with rabbit leukocyte differentiation molecules (LDM). Screening sets of mAbs, developed against LDM in other species, for reactivity with rabbit LDM yielded 11 mAbs that recognize conserved epitopes on rabbit LDM orthologues and multiple mAbs that recognize epitopes expressed on the major histocompatibility class I or class II molecules. Screening of mAbs submitted to the Animal Homologues Section of...
10.

Comparative efficacy of standard AGID, CCIE and competitive ELISA for detecting bluetongue virus antibodies in indigenous breeds of sheep and goats in Rajasthan, India

Smriti Shringi, B. N. Shringi 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 12 77-79 (3 pages)

as a reference test. These results indicate that standard AGID may generate false negative results thereby increasing the chances that the animals with previous exposure to BTV infection may be detected as negative reactors. On the contrary, the cELISA has an advantage of being 100% sensitive as well as specific because it measures BTV-specific antibody without detecting cross-reacting antibody to...
- 1,500원
of the standard AGID, counter current immuno-electrophoresis (CCIE) and the competitive enzyme-linked immunosorbent assay (cELISA) for the detection of serum antibody against BTV in indigenous breeds of sheep were compared. Out of 178 sheep serum samples tested, 17 (9.5%), 22 (12.3%) and 54 (30.3%) were positive for group-specific bluetongue antibodies by AGID, CCIE and cELISA, respectively. There was appreciable difference in the seroprevalence detected by AGID, CCIE and cELISA in clinically...

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