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Examining the possibilities for children’s digital literacy practices at home: Two theoretical perspectives
Lisa Kervin 경인교육대학교 교육연구원 The Journal of Education 14 Pages
경인교육대학교 교육연구원 The Journal of Education 2020, Vol.3 No.2 6 95-108 (14 pages)
strong b gs together th of play. The r and focus them ation, collabor practices at hom eriods of direc re the participa n person. throughout the ctions where t unfold and do ctivities fo eting for a p instances of th home ds in time. The he long “ahhh” music is fun bass line. The he environmen rhythmic repet m on the intera ration and the me: ct gaze. The ants gaze is e video text. the... The everyday use of digital technologies influences the literacy practices of young children and their families. Literate knowledge, skills, and understandings emerge through interactions between elements of the environment, artefacts or ‘tools’ (digital and non-digital), and people. As technologies become increasingly portable, affordable and efficient there is need to examine how digital experiences contribute to emergent literacy development. I draw upon the 2020 Australian COVID-19... -
Genetic diversity and virulence of Streptococcussuis from diseased pigs in Korea
Ho Seong Cho 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 218 452-453 (2 pages)
symptoms , strong contagiousness, high mortality rate and has been expanded globally. Additionally, there is no treatment and vaccine. So, the ASF is classified as notifiable disease by the World Organisation for Animal Health(OIE). At present, the Republic of Korea is ASF free country but there are many risks of introduction from affected countries. To maintain ASF free country, continuous... -
Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera
Hyojin Lee, Eun-Ju Kim, Jae-Young Song, Jeong Soo Choi, Ji Youn Lee, In-Soo Cho, Yeun-Kyung Shin 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 6 307-314 (8 pages)
of the positive serum samples was 1/80, while less diluted sera (1/10 and 1/50) showed strong background signals in the IFA. ANOVA and the Mann-Whitney rank sum test, with p values ≤ 0.001 indicating a significant difference between groups (SYSTAT ver. 4.0; Systat Software, USA). Bee swarm and box plots indicating the distributions of negative or positive groups were generated using an online... Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV), a phlebovirus in the family Bunyaviridae, is an emerging tick-borne infectious disease that impacts humans. This disease manifests as a decreased blood cell count and multi-organ failure, with a case-fatality rate of more than 12% in China. Because vaccines or antiviral drugs for the treatment of this disease are not available, monitoring the SFTS circulation in animals and controlling the tick-mammal cycle are... -
Hydration status affects osteopontin expression in the rat kidney
Su-Youn Lee, Sae-Jin Lee, Hong-Lin Piao, Suk-Young Yang, I. David Weiner, Jin Kim, Ki-Hwan Han 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 2 269-277 (9 pages)
In addition, strong labeling was present in thick ascending limb segments, notably at the apical surface of the cells (panel E in Fig. 2). Occasionally, a few cells ..PAGE:5 Hydration status affects OPN expression 273 www.vetsci.org Fig. 3. Light micrographs illustrating OPN immunostaining in the cortex (A). Occasionally, OPN expression was observed in Bowman’s capsules (B, arrows) and distal... Osteopontin (OPN) is a secretory protein that plays an important role in urinary stone formation. Hydration status is associated with the development of urolithiasis. This study was conducted to examine the effects of dehydration and hydration on OPN expression in the rat kidney. Animals were divided into three groups, control, dehydrated, and hydrated. Kidney tissues were processed for light and electron microscope immunocytochemistry, in situ hybridization, and immunoblot analysis. Dehydration... -
Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)
than the bMGEs groups. In addition, the rate of geneticin-resistant colony formation from the hTERT-bMGEs group was higher than that in the literature [18], which might have been due to the strong proliferation ability of hTERT-bMGEs. Furthermore, the exogenous protein hLYZ maintained stable expression in positive hTERT-bMGEs by RT-PCR and western blot analysis. Therefore, positive-hLYZ... Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated... -
Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Alisha Wehdnesday Bernardo Reyes, Hannah Leah Tadeja Simborio, Huynh Tan Hop, Lauren Togonon Arayan, Suk Kim 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 16 119-122 (4 pages)
any host species [4]. Current serological tests are relatively accessible and cheap, more reliable, generally approved and often the preferred method for diagnosis of brucellosis [1]. Despite the strong immunoreactivity of the antigen used in these classical serological tests, which is commonly derived from smooth lipopolysaccharide (LPS) or O-polysaccharide, its specificity is very low an... The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use... -
Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage
Li-ming Wu, Rui Guo, Lin Hui, Yong-gang Ye, Jing-mei Xiang, Chun-yun Wan, Miao Zou, Rui Ma, Xiao-zhuan Sun, Shi-jin Yang, Ding-z 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 3 475-483 (9 pages)
F in Fig. 1). Results of the immunocytochemical staining experiment indicated that the primary IECs and IEC-6 cells exhibited strong immunoreactivity specific for PCK and CK18 (panels A1, A2, C1, and C2 in Fig. 2) but not vimentin (panels A and C in Fig. 2). In contrast, only yellow staining corresponding to vimentin was found in the V79 cells (panel B in Fig. 2); immunoreactivity against... Chronic enteritis can produce an excess of reactive oxygen species resulting in cellular damage. Stanniocalcin-1(STC-1) reportedly possesses anti-oxidative activity, the aim of this study was to define more clearly the direct contribution of STC-1 to anti-oxidative stress in cattle. In this study, primary intestinal epithelial cells (IECs) were exposed to hydrogen peroxide (H2O2) for different time intervals to mimic chronic enteritis-induced cellular damage. Prior to treatment with 200 μM... -
Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) from cattle in Korea between 2010 and 2011
Eun Kang, Sun Young Hwang, Ka Hee Kwon, Ki Yeon Kim, Jae Hong Kim, Yong Ho Park* 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 5 369-379 (11 pages)
for 1 min on the slide glass. Only strong agglutination observed within 1 min in the reaction with each serum was regarded as positive. Positive samples were then tested with each monovalent serum consisting of the polyvalent serum. The mixture of 0.85% saline and serum was used as a negative control. Determination of the H-antigen was carried out using the test tube method with the bacteria... A total of 156 Shiga-like toxin producing Escherichia coli (STEC) were isolated from fecal samples of Korean native (100/568, 18%) and Holstein dairy cattle (56/524, 11%) in Korea between September 2010 and July 2011. Fifty-two STEC isolates (33%) harbored both of shiga toxin1 (stx1) and shiga toxin2 (stx2) genes encoding enterohemolysin (EhxA) and autoagglutinating adhesion (Saa) were detected by PCR in 83 (53%) and 65 (42%) isolates, respectively. By serotyping, six STEC from native cattle and... -
Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus
Yi-Yang Lien, Chi-Hung Huang, Fang-Chun Sun, Shyang-Chwen Sheu, Tsung-Chi Lu, Meng-Shiunn Lee, Shu-Chin Hsueh, Hsi-Jien Chen, Me 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 11 73-79 (7 pages)
IHC and immunochromatographic assay. First, four CAV-infected chicken livers were analyzed using IHC; all of the paraffin-embedded tissues were positive. The positive control slides demonstrated strong immunoreactivity against CAV antigen (Fig. 3B). In addition, four CAV mock-infected chicken livers were analyzed as the negative control; these were all negative for immunoreactivity against... Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd129, was cloned into an Escherichia (E.) coli expression vector. After... -
Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice
Sung Jae Shin, Seung Won Shin, Mi Lan Kang, Deog Yong Lee, Moon-Sik Yang, Yong-Suk Jang, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 11 383-392 (10 pages)
to the pigs. Mice immunized with pro- teins extracted from yeast expressing the ApxIA antigen produced strong IgG antibody responses and were pro- tected against challenge, which suggests that the rApxIA antigen expressed in S. cerevisiae is highly immunogenic. IgA and IgG immune responses increased following oral vaccination, and the highest level of response was ob- served in the group... We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ApxIIA (group D) alone or both (group E) was compared with that in two control groups (group A and B). The immunogenicity of...