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Correlation between Osmolytes Content with Motility of Canine Spermatozoa after Freezing-thawing with Multistep Method and Different Cryoprotectant Agents
Erif Maha Setyawan, Min Jung Kim, Hyun Ju Oh, Geon A Kim, Young Kwang Jo, Seok Hee Lee, Yoo Bin Choi, Byeong Chun Lee 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 162 223-223 (1 pages)
S. Separation anxiety syndrome in dogs and cats. J Am Vet Med Assoc 2003, 222, 1526-1532. Takeuchi Y, Houpt KA, Scarlett JM. Evaluation of treatments for separation anxiety in dogs. J Am Vet Med Assoc 2000, 217, 342-345. P-141 Correlation between Osmolytes Content with Motility of Canine Spermatozoa after Freezing-thawing with Multistep Method and Different Cryoprotectant Agents Erif Maha... -
Effects of Olfactory and Auditory Stimulation on Separation Anxiety by Salivary Cortisol Measurement in Dogs
Yoon Joo Shin, Nam Shik Shin 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 161 222-223 (2 pages)
S. Separation anxiety syndrome in dogs and cats. J Am Vet Med Assoc 2003, 222, 1526-1532. Takeuchi Y, Houpt KA, Scarlett JM. Evaluation of treatments for separation anxiety in dogs. J Am Vet Med Assoc 2000, 217, 342-345. P-141 Correlation between Osmolytes Content with Motility of Canine Spermatozoa after Freezing-thawing with Multistep Method and Different Cryoprotectant Agents Erif Maha... -
Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system
Wonyou Lee, Kilyoung Song, Inhyung Lee, Hyungdo Shin, Byeong Chun Lee, Seongchan Yeon, Goo Jang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 15 509-516 (8 pages)
to the recipient mare. Statistical analyses All analyses were performed using the Statistical Analysis System (ver. 9.1; SAS Institute, USA). Data were analyzed using a general linear model followed by the least significant difference mean separation procedure when treatments differed at p 0.05. Percentage data were arcsine transformed prior to analysis to maintain homogeneity of variances.... Transvaginal ultrasound-guided follicle aspiration is one method of obtaining recipient oocytes for equine somatic cell nuclear transfer (SCNT). This study was conducted: (1) to evaluate the possibility of oocyte aspiration from pre-ovulatory follicles using a short disposable needle system (14-G) by comparing the oocyte recovery rate with that of a long double lumen needle (12-G); (2) to investigate the developmental competence of recovered oocytes after SCNT and embryo transfer. The recovery... -
A novel mycotoxin purification system using magnetic nanoparticles for the recovery of aflatoxin B1 and zearalenone from feed
Hyun-Jung Kim, Sung-Hee Kim, Jin-Kyu Lee, Cheong-Up Choi, Hee-Soo Lee, Hwan-Goo Kang*, Sang-Ho Cha* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 5 363-369 (7 pages)
M pyridine, ..PAGE:3 Novel mycotoxin purification system using magnetic nanoparticles 365 Fig. 2. Separation of MNPs using a magnet. pH 6.0). A glutaraldehyde solution (5%, 1 mL) was added and incubated with the MNPs at room temperature for 30 min. The particles were washed with wash buffer (0.01 M Tris, 0.15 M NaCl; and 0.1% BSA, pH 7.4) by magnetic separation (Fig. 2). mAb coupling was... that of IAC-ZEN (86∼88% and 81∼88%) for ZEN (10, 25, 50 ng/mL) except at a concentration of 10 ng/mL, demonstrating the remarkable purification efficiency of the novel fMA-mAb method. Additionally, mycotoxin purification was much faster using our novel method (approx. 5 min) than the IAC-based technique (> 30 min). This study suggests that the novel purification system we developed would be a useful tool for monitoring and regulating mycotoxin contamination in feed, and replace IAC methods. -
Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey
G?khan Inat*, Belgin Siriken 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 7 321-326 (6 pages)
Article *Corresponding author Tel: +90-362-312-19-19-3957; Fax: +90-362-457-69-22 E-mail: gkhaninat@yahoo.com Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey Gokhan Inat*, Belgin Siriken Food Hygiene and Technology Department, Faculty of Veterinary... of 100 slaughtered cattle from two commercial abattoirs were tested using the immunomagnetic separation technique and multiplex PCR methods. E. coli O157 and E. coli O157:H7 were detected in 52 of the 200 samples (26%) tested. Of the positive samples, 49 were E. coli O157 and three were E. coli O157:H7. The E. coli O157 strain was isolated from 24 carcasses and 25 rectal samples, while E. coli O157:H7 was isolated from two carcasses and one rectal sample. Of the 49 samples positive for E. coli... -
Rigid External Distraction System을 이용한 구순구개열 상악열성장의 치료 후 회귀현상
도형식, 송영일, 장환용, 이진용, 장현석, 임재석, 권종진, 이의석 대한구순구개열학회 대한구순구개열학회지 10 Pages
대한구순구개열학회 대한구순구개열학회지 2013, 제 16권 1호 2 9-18 (10 pages)
Maxillary Hypoplasia with Cleft Lip and Palate by Rigid External Distraction System Hyoung-Sik DO, Young-Il Song, Hwan-Yong Jang, Jin-Yong Lee, Hyun-Seok Jang, Jae-Suk Rim, Jong-Jin Kwon, Eui-Seok Lee* Department of Oral and Maxillofacial Surgery, Korea University Distraction osteogenesis is usef ul treatment which the gradual separation of cut bone edges results in the generation of new bone.... -
Maternal Social Separation of Adolescent Rats Induces Hyperactivity and Anxiolytic Behavior
HyongRyolKwak, JaeWonLee, Kwang-JunKwon, ChangDonKang, IlYoungCheong, WanjooChun, Sung-SooKim, HeeJaeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.2 2 79-84 (6 pages)
such as maternal and social separation plays an essential role in the development of the nervous system. Adolescent Sprague-Dawley rats that were separated on postnatal day 14 from their dam and litters (maternal social separation, MSS) showed hyperactivity and anxiolytic behavior in the open field test, elevated plus-maze test, and forced-swim test. Biologically, the number of astrocytes was... such as maternal and social separation plays an essential role in the development of the nervous system. Adolescent Sprague-Dawley rats that were separated on postnatal day 14 from their dam and litters (maternal social separation, MSS) showed hyperactivity and anxiolytic behavior in the open field test, elevated plus-maze test, and forced-swim test. Biologically, the number of astrocytes was significantly increased in the prefrontal cortex of MSS adolescent rats. The hyperactive and anxiolytic... -
Inhibitory Activity of Sedum middendorffianum-Derived 4-Hydroxybenzoic Acid and Vanillic Acid on the Type III Secretion System of Pseudomonas syringae pv. tomato DC3000
Ji Eun Kang, Byeong Jun Jeon, Min Young Park, Beom Seok Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 8 608-617 (10 pages)
methanol. The sephadex fraction with T3SS inhibitory effect was concentrated in vacuo and dissolved in high-performance liquid chromatography (HPLC) grade methanol. Afterwards, preparative HPLC separation with Cosmosil 5C18-MS-II column (10 × 150 mm) was carried out at a rate of 3 ml/min with a linear gra- dient of solvent from 20% aqueous methanol (0.04% TFA) to 35% aqueous methanol (0.04%... The type III secretion system (T3SS) is a key virulence determinant in the infection process of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). Pathogen constructs a type III apparatus to translocate effector proteins into host cells, which have various roles in pathogenesis. 4-Hydroxybenozic acid and vanillic acid were identified from root extract of Sedum middendorffianum to have inhibitory effect on promoter activity of hrpA gene encoding the structural protein of the T3SS apparatus. The... -
Characterization of Type VI Secretion System in Xanthomonas oryzae pv. oryzae and Its Role in Virulence to Rice
Yeounju Choi, Namgyu Kim, Mohamed Mannaa, Hongsup Kim, Jungwook Park, Hyejung Jung, Gil Han, Hyun-Hee Lee, Young-Su Seo 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 3호 9 289-296 (8 pages)
of variance was performed using the general linear model, and separation of means was determined by Tukey's Honest Significant Difference test, setting P < 0.05 as the statistical significance threshold. The growth rates and bacterial swarming motility of Δhcp1, Δhcp2, and Δhcp12 mutant strains were not signifi- cantly different from those of the wild-type (Fig. 2). These results indicated... Type VI secretion system (T6SS) is a contact-dependent secretion system, employed by most gram-negative bacteria for translocating effector proteins to target cells. The present study was conducted to investigate T6SS in Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial blight in rice, and to unveil its functions. Two T6SS clusters were found in the genome of Xoo PXO99A. The deletion mutants, Δhcp1, Δhcp2, and Δhcp12, targeting the hcp gene in each cluster, and a double-deletion... -
Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)
of electrophoresis (40 min) for clear separation of the sub- types (Fig. 2C-NdeI and Fig. 3-NdeI). The relationships between restriction maps and different RSV subtypes are shown in Table 1. Origin of RSV in Korea. The origin of RSV in Korea is not fully understood. To clarify this point, we tested individual migratory SBPHs caught by aerial net traps in early June in JB and CN provinces. These... reverse transcription- polymerase reaction/restriction enzyme (RTPCR/ RE) analysis-based method. We identified five REs, including DraI, EcoR1, NdeI/AseI, and SpeI, that could differentiate RSV RNA 1-4 subtypes, respectively. Our RT-PCR/RE results provided a clear pattern of RNA reassortment, i.e., different groups of isolates having their RNA segments derived from two to three different RSV ancestors, such as from Eastern and Southwestern Chinese or Japanese M and T isolates. We also found that...


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