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Gene Expression Alterations between a Persistently PrPBSE Infected and Rubus coreanus Miquel - cured M2B Cell Line
Hyo Jin Kim, Hyun Jeong Kim, In Soon Roh, Won Yong Lee, Tae Young Suh, Hoo Chang Park, Byoung han Kim, Hyun Joo Sohn 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 138 404-405 (2 pages)
complete blood count (CBC) analysis. CBC included white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration (Hgb) and haematocrit (HCT). The presence of antibodies against PRRSV was tested by commercialized ELISA Kit (IDEXX). Results: No significant correlations were found between specific gene modification and PRRS antibody positive status. However, the high RBC,... -
Inhibitory Effects of Nampungchal Sorghum Ethanol Extract on Proliferation in PC-3 cells via apoptosis and cell cycle arrest
Jae Myun Ryu, Gwi Yeong Jang, Heon Sang Jeong, Dae Joong Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 101 139-140 (2 pages)
cell count kit-8. To confirm apoptosis and cell cycle arrest, flow cytometry analyses were performed with annexin V ap optosis detection kit or propidium iodide stain, respectively. The expression levels of proteins related with apoptosis and cell cycle arrest were analyzed by western blot. Results: The viability of PC-3 cells was decreased in dose-dependent manner by NSE-EtOH. The rates of... -
The effect of intravenous injection of human adipose derived stem cell conditioned medium on ovulation and subsequent embryo development in aged mice
Kihae Ra, Geon A Kim, Hyun Ju Oh, Sung Keun Kang, Jeong Chan Ra, Byeong Chun Lee 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 316 381-382 (2 pages)
to count RBCs by hemocytometer. To estimate the effect of RBCs in plasma on clinical biochemistry, blood samples were centrifuged at 2786 g for 5 minutes by the conventional centrifuge. The blood plasma was processed to contain 50000, 5000, 500, and 50 RBC/microliter. Each sample was repeatedly measured in the duplication with Catalyst Dx Chemistry Analyzer (IDEXX, USA) and SAMSUNG PT10V... -
Clinical assessment after human adipose stem cell transplantation into dogs
Seok Hee Lee, Erif M.N. Setyawan, Yoo Bin Choi, Jeong Chan Ra, Sung Keun Kang, Byeong Chun Lee, Geon A Kim 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 15 452-461 (10 pages)
and 21 (ASC infusion group 13.9 ± 2.9 and 10.9 ± 3.0 pg/mL, respectively; ASC bolus group ..PAGE:5 456 Seok Hee Lee et al. Journal of Veterinary Science Fig. 3. Evaluation of complete blood cell count after saline or adipose tissue-derived stem cell (ASC) transplantation. All groups were within normal ranges. 1, 7, 14, 21, 28 days: time after the saline or ASC transplantation (infusion rate, 3... because of their stemness characteristics. However, little has reported on evaluating adverse responses caused by human ASC therapy. Therefore, in the present study, a clinical assessment after human ASC transplantation into dogs was undertaken. A total of 12 healthy male dogs were selected and divided into four groups: saline infusion, saline bolus, ASC infusion, and ASC bolus groups. Physical assessment and blood analysis were performed following ASC transplantation, and the concentrations of... -
Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression
Tomohiro Osaki, Yuji Sunden, Akihiko Sugiyama, Kazuo Azuma, Yusuke Murahata, Takeshi Tsuka, Norihiko Ito, Tomohiro Imagawa, Yosh 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 16 385-390 (6 pages)
in 2 mL of cultivation medium for 24 h. Following incubation, cells were harvested and prepared for daily cell counts, which were conducted using a Muse Count & Viability Kit (EMD Millipor e, USA) according to the instructions supplied by the manufacturer. Cells were co-incubated with Muse Count & Viability Reagent at room temperature for 5 minutes in the dark. Single-cell suspensions were... a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdc/J mouse at the site of subcutaneous SNP cell injection. SNP cells are characterized by proliferation in a tubulopapillary pattern and are vimentin positive. Moreover, we examined miRNA expression in the cultured cells and found that the expression values of miRNA-143 and miRNA-138a showed the greatest increase and decrease, respectively, of all miRNAs observed, indicating that these miRNAs might play a... -
Primary intestinal mast cell tumor in a Russian Blue cat: ultrasonographic and histopathological findings
Seong-jun Kim , Jun Jaekal , Jun-young Kim , Dong-keun Oh , Jun-ho Cho , Min-hee Kang , Hee-myung Park 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 3 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 2호 9 131-133 (3 pages)
complete blood cell count and serum chemistry profile indicated polycythemia (hematocrit 55.7%; reference range, 24.045.0), hyperpro- teinemia (total protein 11 g/dL; reference range, 5.77.8), increased levels of liver enzymes (alanine transaminase [ALT] 315 U/L; reference range, 2284; aspartate transaminase [AST] 168 U/L; reference range; 1851; alkaline phosphatase [ALP] 459 U/L; reference range,... -
Classification of acute clinical mastitis on the base of vital signs and complete blood count test in dairy cows
Tai Young Hur , Seog Jin Kang , Young Hun Jung , Hyun June Lee , Kwang Seok Ki , Chang Yong Choe , Guk Hyun Suh 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2013, 제 53권 제 1호 4 19-23 (5 pages)
20 Tai-Young Hur, Seog-Jin Kang, Young-Hun Jung, Hyun-June Lee, Kwang-Seok Ki, Chang-Yong Choe, Guk-Hyun Suh cows. The mean somatic cell count for the herd’s milk ranged from 150,000 to 300,000 cell/mL. Cows were fed a total mixed ration based on production levels and were brought to parlor 2 times a day. All cows were housed in free stalls and were not vaccinated against bovine mastitis. Farm... -
Multicentric T cell Lymphoma in a Maltese dog
JiYoulJung, SangChulKang, InSoonRoh, HyunJooSohn, YoungMinYun, JungHunKim, KyoungKapLee, MinChanAn, JongHeeBae, JaeHoonKim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2007, 제 47권 제 1호 11 85-89 (5 pages)
complete blood cell (CBC) count revealed anemia with a packed cell volume of 15%. Abnormal serum biochemical results included elevated alanine transferase (126 U/L), alkaline phosphatase (2,000 U/L), total bilirubin (1.24 mg/dl), and total cholesterol (412.5 mg/dl). The dog was unresponsive to symptomatic, fluid and chemotherapies. Because of poor prognosis the dog was euthanized and submitted to... -
Nafamostat Mesilate Inhibits TNF-α-Induced Vascular Endothelial Cell Dysfunction by Inhibiting Reactive Oxygen Species Production
Min-WoongKang, Hee-JungSong, ShinKwangKang, YonghwanKim, Saet-byelJung, SungjuJee, JaeYoungMoon, Kwang-sunSuh6, SangDoLee, ByeongHwaJeon, 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.3 6 229-234 (6 pages)
on HUVEC viability were examined using an ADAM-MC automatic cell count er (Digital Bio, Seoul, South Korea), which assesses propidium iodide (PI) stain- ing [14]. Antibodies and immunoblotting Anti-VCAM-1, anti-ICAM-1, and anti-β-actin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-shcA antibodies were purchased from Becton Dickinson (Fullerton, CA, USA).... monocyte adhesion, vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) protein expression, p38 mitogenactivated protein kinase (MAPK) activation, and intracellular superoxide production were then examined. NM (0.01∼100 μg/mL) did not affect HUVEC viability; however, it inhibited the increases in reactive oxygen species (ROS) production and p66shc expression elicited by TNF-α (3 ng/mL), and it dose dependently prevented the TNF-α-induced upregulation of... -
Neuroprotective Effect of Visnagin on Kainic Acid-induced Neuronal Cell Death in the Mice Hippocampus
Min-SooKwon, Jin-KooLee, Soo-HyunPark, Yun-BeomSim, Jun-SubJung, Moo-HoWon, Seon-MiKim, Hong-WonSuh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.5 2 257-263 (7 pages)
o f the hippocampal CA3 region per- forming cresyl violet-positive neu- ronal count is indicated (A) refer- ring Franklin [18]. I.c.v. kainic acid injecton induced neuronal cell death in the pyramidal cells in the CA3 region of hippocampus (B). Mice were administered visnagin orally (C) or intraperitoneally (D) 20 min (20) prior to KA (0.1μg / 5 μl) injection or 0, 20 (+20), 40 (+40), 60... neuroprotective effect against kainic acid (KA)-induced neuronal cell death. In the cresyl violet staining, pre-treatment or post-treatment visnagin (100 mg/kg, p.o. or i.p.) showed a neuroprotective effect on KA (0.1Ռg) toxicity. KA-induced gliosis and proinflammatory marker (IL-1Ղ, TNF-Ձ, IL-6, and COX-2) inductions were also suppressed by visnagin administration. These results suggest that visnagin has a neuroprotective effect in terms of suppressing KA-induced pathogenesis...


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