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전체 선택해제
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본문검색 결과만 보기
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A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis
Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 9 233-239 (7 pages)
The final PCR results for 11 samples analyzed with the QIAamp and UACH methods or ones examined with the QIAamp and CTAB methods were in agreement. Complete (100%) agreement was observed between data from the CTAB and UACH methods. CP values for the UACH and CTAB techniques were not significantly different, while the UACH method yielded significantly lower CP values compared to the QIAamp kit. The proposed extraction method combines reliability and efficiency with simplicity and lower cost. -
Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay
Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 8 143-149 (7 pages)
The best enrichment broth and DNA extraction scheme was determined for rapid and sensitive detection of Salmonella Enteritidis in steamed pork using real-time PCR. The inhibitory effect of commonly used Salmonella enrichment broths, Rappaport-Vassiliadis (RV) and Muller-Kauffmann tetrathionate with novobiocin (MKTTn), on real-time PCR was confirmed. The inhibition of PCR was statistically significant (p ＜ 0.05) in RV and MKTTn, as compared with buffered peptone water (BPW) or phosphate-buffered... -
Short Communication : Effective DNA extraction method to improve detection of Mycobacterium avium subsp. paratuberculosis in bovine feces
Hong Tae Park , Min Kyoung Shin , Kyung Yong Sung , Hyun Eui Park , Yong Il Cho , Han Sang Yoo 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 3 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2014, 제 54권 제 1호 10 55-57 (3 pages)
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Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 15 289-292 (4 pages)
the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enzymes, and Southern hybridization indicated that polysaccharides were effectively removed by this method, and the resulting DNA was sufficient for use in subsequent molecular analysis. -
A Quick and Safe Method for Fungal DNA Extraction
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 1호 17 108-111 (4 pages)
including cloning and genotyping, have become routine in fungal research laboratories. However, preparation of high-quality DNA from fungal tissue requires much time and labor and is often a limiting step for high-throughput experiments. We have developed a quick and safe (QS) DNA extraction method for fungi. Time efficiency and safety in the QS method were achieved by using plate-grown mycelia as the starting material, by eliminating phenol-chloroform extraction procedures, and by deploying a... -
A Simple Method for Extraction of High Molecular Weight DNA fromPorphyra Tenera (Rhodophyta) Using Diatomaceous Earth
김태훈, 황미숙, 송주동, 오민혁, 문용환, 정익교, 류태형, 이춘환, Kim. Tae-Hoon, Hwang. Mi-Sook, Song. Ju-Dong, Oh. Min-Hyuk, Moon. Yong-Hwan, Chung. Ik-Kyo, Rhew. Tae- 한국조류학회(藻類) Algae 6 Pages
한국조류학회(藻類) Algae 2006, Vol.21 No.2 261-266 (6 pages)
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A Simple Method for DNA Extraction from Red Algae
이유경, 이홍금, Lee. Yu Gyeong, Lee. Hong Geum 한국조류학회(藻類) Algae 5 Pages
한국조류학회(藻類) Algae 2003, Vol.18 No.1 65-69 (5 pages)
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Direct Extraction of DNA from Soil for Amplification of 16S rRNA Gene Sequences by Polymerase Chain Reaction
Cho. Jae-Chang, Lee. Dong-Hun, Cheol. Cho-Young, Cho. Jang-Cheon, Kim. Sang-Jong 한국미생물학회 The journal of microbiology 7 Pages
한국미생물학회 The journal of microbiology 1996, Vol.34 No.3 229-235 (7 pages)
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An Easy, Rapid, and Cost-Effective Method for DNA Extraction from Various Lichen Taxa and Specimens Suitable for Analysis of Fungal and Algal Strains
Park. Sook-Young, Jang. Seol-Hwa, Oh. Soon-Ok, Kim. Jung A, Hur. Jae-Seoun 한국균학회 Mycobiology 6 Pages
한국균학회 Mycobiology 2014, Vol.42 No.4 311-316 (6 pages)
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Detection of Mycobacterium bovis in the lymph node of tuberculin positive cattle by guanidium isothiocyanate/silica DNA extraction and polymerase chain reaction
Cho. Yun-Sang, Jung. Suk-Chan, Yoo. Han-Sang, Kim. Jong-Man 한국가축위생학회 韓國家畜衛生學會誌 9 Pages
한국가축위생학회 韓國家畜衛生學會誌 2007, Vol.30 No.2 233-241 (9 pages)
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Effective Method for Extraction of Cell-Free DNA from Maternal Plasma for Non-Invasive First-Trimester Fetal Gender Determination: A Preliminary Study
Lim. Ji-Hyae, Park. So-Yeon, Kim. Shin-Young, Kim. Do-Jin, Kim. Mee-Jin, Yang. Jae-Hyug, Kim. Moon-Young, Kim. Min-Hyoung, Han. Ho-Won, C 대한의학유전학회 Journal of genetic medicine 6 Pages
대한의학유전학회 Journal of genetic medicine 2010, Vol.7 No.1 53-58 (6 pages)
정량 형광-중합효소 연쇄 반응(QF-PCR)을 이용하여SRY 와AMXY 유전자를 검출하였다. QF-PCR의 진단 정확도는 최종 분만 기록을 토대로 결정하였다. 결 과: 전체 44명의 여성이 실험에 참여하였지만, 최종 분만 기록은 단지 36명의 여성에서 획득하였다. 이들 중 16명은 남아를 20명은 여아를 임신하였다. 두 추출 방법에서 태아 성별의 진단적 정확도는 일반 혈액 DNA 추출 방법에 경우 63.9% (23/26)였으며 viral DNA 추출 방법에 경우 97.2% (35/36) 였다. 결 론: QF-PCR을 이용한 비침습적 임신초기 태아 성별 확인에 있어 viral kit를... -
Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method
김재환, 김미아, 이대우, 백병주, 양연미, 김재곤, Kim. Jaehwan, Kim. Miah, Lee. Daewoo, Baik. Byeongju, Yang. Yeonmi, Kim. Jaegon 대한소아치과학회 大韓小兒齒科學會誌 6 Pages
대한소아치과학회 大韓小兒齒科學會誌 2014, Vol.41 No.4 292-297 (6 pages)
외의 방법은 페놀과 클로로포름과 같은 유해한 화학물질을 써야하는 등의 단점이 있다. 이 연구에서 NaOH 용액을 이용한 개선된 DNA 추출 방법은 치아우식증, 치주염과 관련된 병원체를 빠르고 간단하며 비용-효율적으로 검출하였다. 세균으로부터 DNA를 추출하기 위한 boiling은 기존의 10분이 아닌 1분으로 충분하였고 $4^{circ}C$에서 최소 13개월 이상 DNA의 보관이 가능하였으며 sonication 유무에 따른 차이는 없었다. 따라서, 이 방법은 상업적인 kit나 유해한 화학물질을 쓰지 않고서도 타액 표본으로부터 직접적으로 빠른 시간... -
Imported Malaria in United Arab Emirates: Evaluation of a New DNA Extraction Technique Using Nested PCR
Sultan. Doaa M., Khalil. Marwa M., Abdouh. Ahmed S., Doleh. Wafaa F., AI Muthanna. Abdul Aziz M. 대한기생충학회 The Korean journal of parasitology 7 Pages
대한기생충학회 The Korean journal of parasitology 2009, Vol.47 No.3 227-233 (7 pages)
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Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes
Masoumeh Firouzamandi, Hassan Moeini, Davood Hosseini, Mohd Hair Bejo, Abdul Rahman Omar, Parvaneh Mehrbod, Aini Ideris 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 3 21-26 (6 pages)
The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant...
