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Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides
Hong-tao Chang, Xiu-yuan He, Yu-Feng Liu, Lu Chen*, Quan-hai Guo, Qiu-ying Yu, Jun Zhao, Xin-wei Wang, Xia Yang, Chuan-qing Wang 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 8 399-407 (9 pages)
(PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG... -
Genetic characterization of porcine circovirus - 2 field isolates from PMWS pigs
Jin H. Kim, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 6 31-39 (9 pages)
result indicates that although the genome of PCV-2 is relatively stable in general, minor genetic variations exist among PCV-2 isolates from the different geographic locations. These differences of viral genome might have an important implication for genetic characteristics of PCV-2 infection. Three major immunorelevant epitopes of capsid protein showed variations in amino acid sequences. Also, the variance of amino acid sequence in antigenic epitope existed between two Korean PCV-2 isolates. -
Porcine circovirus type 2 increases interleukin-1beta and interleukin-10 production via the MyD88-NF-kappa B signaling pathway in porcine alveolar macrophages in vitro
Junyuan Han, Shuxia Zhang, Yaqun Zhang, Mengmeng Chen, Yingjun Lv 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 8 183-191 (9 pages)
Porcine alveolar macrophages (PAMs) represent the first line of defense in the porcine lung after infection with porcine circovirus type 2 (PCV2) via the respiratory tract. However, PCV2 infection impairs the microbicidal capability of PAMs and alters cytokine production and/or secretion. At present, the reason for the imbalance of cytokines has not been fully elucidated, and the regulatory mechanisms involved are unclear. In this study, we investigated the expression levels and regulation of... -
돼지 써코바이러스 2형 유전형 분류를 위한 nested-PCR 적용
추금숙, Chu. Keum-Suk 한국가축위생학회 韓國家畜衛生學會誌 6 Pages
한국가축위생학회 韓國家畜衛生學會誌 2011, Vol.34 No.1 13-18 (6 pages)
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전북 익산지역 양돈장에서 돼지생식기호흡기 증후군, 써코바이러스-2 및 마이코프라즈마 폐렴의 항체가 조사
추금숙, 형상기, 이지영, 김지영, 서이원, 정병우, Chu. Keun-Suk, Hyong. Sang-Gi, Lee. Ji-Young, Kim. Ji-Young, Seo. Lee-Weon, Jung. Bung-Woo 한국가축위생학회 韓國家畜衛生學會誌 8 Pages
한국가축위생학회 韓國家畜衛生學會誌 2007, Vol.30 No.3 305-312 (8 pages)
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The Current Status of Swine Respiratory Diseases in South Korea: Porcine Respiratory and Reproductive Viruses (PRRSVs) and Porcine Circovirus Type 2 (PCV)
Ga Eun Lee, Jung Ah Kim, Chan Hee Lee, Hee Chun Chung, Hye Jung Yang, Bong Kyun Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 53 111-112 (2 pages)
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Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays
In-Soo Choi and Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 10 207-212 (6 pages)
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by... -
Preliminary assessment of correlation between T-lymphocyte responses and control of porcine reproductive and respiratory syndrome virus (PRRSV) in piglets born after in-utero infection of a type 2 PRRSV
Sang-ho Cha , Carey Bandaranayaka-mudiyanselage , Chandima B. Bandaranayaka-mudiyanselage , Dharani Ajiththos , Kyoung-jin 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2018, 제 58권 제 1호 2 9-16 (8 pages)
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구강액을 이용한 양돈장의 Porcine circovirus-2 감염에 대한 모니터링
김원일, Kim. Won-Il 한국임상수의학회 Journal of veterinary clinics 9 Pages
한국임상수의학회 Journal of veterinary clinics 2010, Vol.27 No.6 704-712 (9 pages)
Porcine circovirus-2 (PCV2) 는 돼지에서 여러 형태의 질병과 증후군의 발생과 관련이 되어있어 현재는 PCV-associated diseases (PCVAD)로 총괄적으로 분류된다. PCVAD에 의한 높은 경제적 손실 때문에 많은 양돈장들이 PCV2의 감염을 확인하기 위하여 혈청을 검사하고 있다. 하지만, 기존의 혈액채취법은 비용이 높고 많은 인력이 소요되므로 큰 규모의 병원체 검사에는 어려움이 있었다. 이에 본 연구에서는 혈액채취법을 이용한 돈군의 PCV2 검사법에 대한 대체방법으로 돈방 단위의 구강액채취법의 유용성을 실제 농장에서... -
가축과 실험동물의 생리자료 (12)-2 - 제12장 쥐(The Rat) -
정순동(Jeong, Sun-Dong), 양일석(Yang, Il-Seok) 대한생리학회 대한생리학회지 11 Pages
대한생리학회 대한생리학회지 1980, 제 14권 제 1호 7 47-57 (11 pages)
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가축과 실험동물의 생리자료(11)-2 -제11장 토끼(The Rabbit)-2-
정순동(Jeong, Sun-Dong), 양일석(Yang, Il-Seok) 대한생리학회 대한생리학회지 6 Pages
대한생리학회 대한생리학회지 1974, 제 8권 제 2호 11 91-96 (6 pages)
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Molecular Characterization of Fusarium Graminearum Virus 2 Isolated from Fusarium graminearum Strain 98-8-60
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 11 285-290 (6 pages)
Fusarium graminearum virus 2 (FgV2) infects Fusarium graminearum strain 98-8-60 and has at least five segments of double-stranded RNAs (dsRNAs), denoted as dsRNA-1 to dsRNA-5. In this study, the genome of FgV2 was sequenced and its phylogenetic relationship with other mycoviruses was analyzed. The lengths of FgV2 dsRNAs 1-5 ranged from 2414 to 3580 base pairs (bp). The 5` and 3` untranslated regions (UTRs) are highly conserved, and each dsRNA segment had 78-105 and 84-306 bp of 5` and 3` UTRs,... -
Assessing PCV2 antibodies in field pigs vaccinated with different porcine circovirus 2 vaccines using two commercial ELISA systems
Min-Kyoung Shin, Seung Hyun Yoon, Myung Hwui Kim, Young Soo Lyoo, Seung Won Suh, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 4 25-29 (5 pages)
Porcine circovirus type 2 (PCV2) is the primary causative agent for post-weaning, multisystemic, wasting syndrome. Consequently, serologic detection of and vaccination against PCV2 are important for the swine industry. Among several serological tests, the enzyme-linked immunosorbent assay (ELISA) is commonly used to measure anti-PCV2 antibody levels. In the present study, we used two commercial ELISA systems to comparatively evaluate anti-PCV2 antibodies in field pigs... -
Pancreatic Lesions of pigs with post-weaning multisystemic wasting syndrome
Ji Youl Jung , Sang Chul Kang , Bong Kyun Park , Eui Kyung Hwang , Dae Yong Kim , Jae Hoon Kim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2010, 제 50권 제 4호 2 265-271 (7 pages)
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Prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in Korea
Kwang Soo Lyoo, Yong Ho Park, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 3호 7 201-207 (7 pages)
infections were investigated as possible causes of the postweaning multisystemic wasting syndrome(PMWS). Specific primers for RT-PCR and PCR were designed for the differential detection of PRRSV, PCV-2 and PPV. Using PCR, these viruses were detected in homogenized tissue samples from pigs that had respiratory of reproductive problems in the time period between 1998 and 2000; the overall prevalences were: PRRSV 31.4%, PCV-2 46.5%, and PPV 8.1%. PCV-2 was also detected in aborted fetal tissues. -
Genetic Characterization of Porcine Circovirus Type 2 Detected from the Pigs in Commercial Swine Farms in Korea
Oh. Sey-Seok, Chu. Jia-Qi, Park. Sang-Hun, Park. Chang-Sik, Kim. Myung-Cheol, Jun. Moo-Hyung 대한미생물학회 Journal of bacteriology and virology : JBV 9 Pages
대한미생물학회 Journal of bacteriology and virology : JBV 2006, Vol.36 No.3 175-183 (9 pages)
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Porcine Circovirus Type 2 Infection in a Piglet Born from a Surrogate Mother
이수형, 강상철, 박준원, 정희천, 박봉균, 김대용, 김재훈, Lee. Su-Hyung, Kang. Sang-Chul, Park. Jun-Won, Chung. Hee-Chun, Park. Bong-Kyun, Kim. Dae-Yong, Kim. Jae-H 한국임상수의학회 Journal of veterinary clinics 4 Pages
한국임상수의학회 Journal of veterinary clinics 2014, Vol.31 No.3 258-261 (4 pages)
림프장기에서는 심한 림프구의 소실과 조직구 또는 다핵세포의 침윤을 보이고 있었다. 면역조직화학염색을 통하여 심장의 괴사된 심근세포, 큰포식세포 및 다핵거대세포와 림프 장기의 림프구소실 영역에서 큰포식세포 및 다핵세포에서 돼지 써코바이러스 2형(PCV-2)의 항원이 검출되었다. 유산 또는 사산된 돼지에서 PCV-2와 관련된 번식장애에서는 심근염이 자주 발생하는 상황이며, 이와 유사한 병변이 PCV-2에 감염된 본 증례의 4주령 돼지에서도 관찰되었다. 이 돼지에서 PCV-2의 감염은 양돈장에서 채취한 난소에 본 바이러스가...
