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본문검색 결과만 보기
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Identification of Plant Viruses Infecting Pear Using RNA Sequencing
Nam-Yeon Kim, Hyo-Jeong Lee, Hong-Sup Kim, Su-Heon Lee, Jae-Sun Moon, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 5 258-267 (10 pages)
overview of virus populations infecting a major pear cultivar (‘Singo’) in Korea. From June 2017 to October 2019, leaf samples (n = 110) of pear trees from 35 orchards in five major pear-producing regions were collected and subjected to RNA sequencing. Most virus-associated contigs matched the sequences of known viruses, including apple stem grooving virus (ASGV) and apple stem pit- ting virus (ASPV). However, some contigs matched the sequences of apple green crinkle-associated virus and... -
The Detection of Plant Viruses in Korean Ginseng (Panax ginseng) through RNA Sequencing
Hong-Kyu Lee, So-Yeon Kim, Hee-Ji Yang, Da-Som Lee, Boram Kwon, Dong-Yun Lee, Jonghee Oh, Su-Heon Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 12 643-650 (8 pages)
A total of 656 leaf samples were combined into one and total RNA was extracted from the polled sample, using RNA sequencing (RNA-Seq), a metatranscriptome analysis of the plant virome was conducted. The virus present in Panax ginseng was confirmed by reverse transcription polymerase chain reaction (RT-PCR) assay using virus-specific primers. In RNA-Seq data analysis, the multiplication protein of four viral contigs including Aristotelia chilensis virus 1 (AcV1), Turnip mosaic virus (TuMV),... -
닭의 성숙/미성숙란에서 RNA Sequencing을 이용한 유전자 발현 양상 고찰
강경수, 장현준, 박미나, 최정우, 정원형, 허강녕, 최창용, 김영주, 이시우, 조은석, 김남신, 김태헌, 한재용, 이경태, Kang. Kyung-Soo, Jang. Hyun-Jun, Park. Mi Na, Choi. Jung-Woo, Chung. Won-Hyo 한국가금학회 한국가금학회지 10 Pages
한국가금학회 한국가금학회지 2014, Vol.41 No.4 287-296 (10 pages)
조사하였다. 본 실험에 사용된 개체는 한국 재래계 흑색계통이 사용되었고, 비교조직은 미성숙란과 성숙란의 RNA를 추출하여 유전자의 발현 양상을 살펴봄으로 난의 성숙에 필요한 유전자의 발현 양상을 보고자 하였다. 실험을 위해 Total RNA를 추출하였고, HiSeq 2000 platform을 사용하여 염기서열을 분석하고, Tuxedo Protocol과 DAVID 프로그램을 통해 유전자의 기능과 상호간의 연관관계를 예측하였다. 탐색된 유전자군은 미성숙란과 성숙란 간에 많은 차이를 보이고 있는 유전자군을 탐색한 결과, 315개의 발현이 다르게 나타나는... -
Original Article : rpoB gene sequencing for phylogenetic analysis of avian pathogenic Escherichia coli
Hyuk Joon Kwon , Won Jin Seong , Tae Eun Kim , Yong Jin Won , Jae Hong Kim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2015, 제 55권 제 1호 5 31-39 (9 pages)
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Comparative Genomic Analysis of Pathogenic Factors of Pectobacterium Species Isolated in South Korea Using Whole-Genome Sequencing
Samnyu Jee, In-Jeong Kang, Gyeryeong Bak, Sera Kang, Jeongtae Lee, Sunggi Heu, Ingyu Hwang 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 1호 2 12-24 (13 pages)
In this study, we conducted whole-genome sequenc- ing with six species of Pectobacterium composed of seven strains, JR1.1, BP201601.1, JK2.1, HNP201719, MYP201603, PZ1, and HC, for the analysis of patho- genic factors associated with the genome of Pectobac- terium. The genome sizes ranged from 4,724,337 bp to 5,208,618 bp, with the GC content ranging from 50.4% to 52.3%. The average nucleotide identity was 98% among the two Pectobacterium species and ranged from 88% to 96% among the remaining... -
Bacterial Community and Diversity from the Watermelon Cultivated Soils through Next Generation Sequencing Approach
Mahesh Adhikari, Sang Woo Kim, Hyun Seung Kim, Ki Young Kim, Hyo Bin Park, Ki Jung Kim, Youn Su Lee 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 3 521-532 (12 pages)
Korea. In this study, soil samples were collected from watermelon cultivation areas from various places of South Korea and microbiome analysis was performed to analyze bacterial communities in- cluding Acidovorax species community. Next generation sequencing (NGS) was performed by extracting genom- ic DNA from 92 soil samples from 8 different provinces using a fast genomic DNA extraction kit. NGS data analysis results revealed that, total, 39,367 operational taxonomic unit (OTU), were obtained.... -
RNA Modification and Its Implication in Plant Pathogenic Fungi
Junhyun Jeon, Song Hee Lee 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 1 505-511 (7 pages)
layer for genetic information, cul- minating in epitranscriptome concept. In plant patho- genic fungi, however, the presence and the biological roles of RNA modifications are largely unknown. Here we delineate types of RNA modifications, and provide examples demonstrating roles of such modifications in biology of filamentous fungi including fungal patho- gens. We also discuss the possibility that RNA modifica- tion systems in fungal pathogens could be a prospective target for new agrochemicals. -
Complete Genome Sequencing and Infectious cDNA Clone Construction of Soybean Mosaic Virus Isolated from Shanxi
Defu Wang, Liyan Cui, Li Zhang, Zhennan Ma, Yanbing Niu 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 7 162-172 (11 pages)
can naturally infect Pinellia ternata and Atractylodes macrocephala. In order to clarify the molecular mechanisms underlying the cross- family infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson assembly tech- niques to carry out SMV full-length genome amplifi- cation from susceptible soybeans and constructed an infectious cDNA clone for SMV. The genome of the SMV Shanxi isolate (SMV-SX) consists of 9,587 nt and encodes... -
Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing
Defu Wang, Liyan Cui, Yanni Pei, Zhennan Ma, Shaofei Shen, Dandan Long, Lingyu Li, Yanbing Niu 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 9 468-475 (8 pages)
rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCVSX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids.... -
Characterization of the Rosellinia necatrix Transcriptome and Genes Related to Pathogenesis by Single-Molecule mRNA Sequencing
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 4호 2 362-369 (8 pages)
of R. necatrix (KACC 40445 strain) using PacBio single-molecule sequencing technology. After annotation of the unigene sets, we selected 14 cell cycle-related genes, which are likely either positively or negatively involved in hyphal growth by cell cycle control. The expression of the selected genes was further compared between two strains that displayed different growth rates on nutritional media. Furthermore, we predicted pathogen-related effector genes and cell wall-degrading enzymes from the... -
Deep Sequencing Analysis of Apple Infecting Viruses in Korea
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 8 441-451 (11 pages)
Deep sequencing has generated 52 contigs derived from five viruses; Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple green crinkle associated virus (AGCaV), and Apricot latent virus (ApLV) were identified from eight apple samples showing small leaves and/or growth retardation. Nucleotide (nt) sequence identity of the assembled contigs was from 68% to 99% compared to the reference sequences of the five respective viral genomes.... -
Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)
a clear pattern of RNA reassortment, i.e., different groups of isolates having their RNA segments derived from two to three different RSV ancestors, such as from Eastern and Southwestern Chinese or Japanese M and T isolates. We also found that the migratory small brown planthopper from Eastern China caught by aerial net traps that possesses RSVRNA3 genotypes corresponds mainly to Eastern China, with a few for Southwestern China based on RT-PCR/ RE, sequence and phylogenetic analyses, indicating... -
Taxonomic Position of Korean Isolates of Rhizoctonia solani Based on RAPD and ITS Sequencing of Ribosomal DNA
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 12 83-89 (7 pages)
Taxonomic position of 46 Korean isolates of Rhizoctonia solani which were classified into nine intraspecific groups by anastomosis and cultural characteristics was analyzed by randomly amplified polymorphic DNA (RAPD) and sequence analyses of the internal transcribed spacer (ITS) regions of ribosomal DNA. All the isolates within each group showed highly similar band patterns in RAPD. The ITS regions of the isolates within the same groups showed a high level of sequence similarity above 96.0%... -
식물 RNA 바이러스의 진화와 병저항성 극복 기작
김명휘, 권선정, 서장균 한국식물병리학회 식물병연구 12 Pages
한국식물병리학회 식물병연구 2021, 27권 4호 2 137-148 (12 pages)
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Targeted RNA Recombination of the Membrane Nucleocapsid Protein Genes between Mouse Hepatitis Virus Bovine Coronavirus
Gabriella Koljesar, Dong Wan Yoo 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 3호 1 149-157 (9 pages)
4 followed by transfection with synthetic RNA transcribed from the DI-like cDNA construct, recombinant mouse hepatitis viruses as the large plaque forming phenotype were isolated by plaque assays at the non-permissive temperature of 391 degrees C. By RT-PCR and sequencing, those large plaque phenotypes were confirmed to have contained the thermostable phenotype marker derived from the transfected RNA, demonstrating that recombination occurred between the Albany 4 genomic RNA and the in vitro RNA... -
Cloning and Sequencing of Coat Protein Gene of the Korean Isolate of Rice stripe virus
한국식물병리학회 The Plant Pathology Journal 3 Pages
한국식물병리학회 The Plant Pathology Journal 2004, 20권 4호 16 313-315 (3 pages)
isolate of Rice stripe virus (RSV-Kr) was cloned and its nucleotide sequence was determined. Total RNA was extracted from infected leaves and RSV viral RNA was detected by using RT-PCR with specific primer of coat protein gene. The result of RT-PCR showed a specific band. Purified RT-PCR products of coat protein gene were ligated into the pGEM-T Easy plasmid vector and cloned cDNA was obtained for nucleotide sequence determination. Coat protein gene of RSV-Kr consisted of 969 bp long encoding a...
