자료유형
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Porphyromonas gingivalis에서의 Hemin 결합 단백질 유전자의 특성 연구
김성조, Kim. Sung-Jo 대한치주과학회 대한치주과학회지 14 Pages
대한치주과학회 대한치주과학회지 1999, Vol.29 No.3 663-676 (14 pages)
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Virulence of Cultured Supernatant in Porphyromonas gingivalis W50 under Hemin- and Menadione-Limited Culture Condition
김강주, 김은철, 김기경, 이현옥, 장선일, 정종평, Kim. Kang-Ju, Kim. Eun-Cheol, Kim. Ki-Gyung, Lee. Hyun-Ok, Jang. Seon-Il, Chung. Chong-Pyoung 대한치주과학회 대한치주과학회지 10 Pages
대한치주과학회 대한치주과학회지 1997, Vol.27 No.3 515-524 (10 pages)
헤민과 메나디온 제한에 의한 Porphyromonas gingivalis(P. gingivalis) W50의 병독력의 변화를 검색하고자, 실험관내 병독력을 NIH 3T3 세포의 세포활성 변화로 관찰하였고, 생체내 병독력은 배양상청액을 ICR mouse 피하조직에 주사한 후의 염증반응을 관찰하였다. 헤민 존재 하에 배양한 P. gingivalis W50 배양상청액에 의한 mouse 3T3 세포의 세포활성은 헤민과 메나디온 없이 배양한 세포의 활성보다 낮았다. 헤민과 메나디온을 첨가하지 않고 배양한 세균의 생체내 병독력은 중등도의 염증세포 침윤과 울혈에 의한 출혈, 미약한... -
Hemin Inhibits Hypertensive Rat Vascular Smooth Muscle Cell Proliferation through Regulation of Cyclin D and P21
Jeon. Eun-Mi, Choi. Hyoung-Chul, Lee. Kwang-Youn, Chang. Ki-Churl, Kang. Young-Jin 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 8 Pages
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 2009, Vol.32 No.3 375-382 (8 pages)
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Tyrosine Kinase is Involved in Hemin-Induced Pyresis
Lee. Sang-Ho, Jang. Choon-Gon, Lee. Seok-Yong 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 5 Pages
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 2003, Vol.26 No.5 411-415 (5 pages)
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Prevotella intermedia에서의 Hemin 결합 단백질 유전자의 분리 및 염기서열 분석
김신, 김성조, Kim. Shin, Kim. Sung-Jo 대한소아치과학회 大韓小兒齒科學會誌 7 Pages
대한소아치과학회 大韓小兒齒科學會誌 2006, Vol.33 No.2 304-310 (7 pages)
P. intermedia chromosomal DNA 내에는 hemin 결합 단백질 유전자가 single copy로 존재하였고, transcript의 크기는 약 1.8kb이었다. 분리한 pHem1 유전자는 1개의 ORF를 가지고 있으며, ORF의 크기는 2,550bp로 약 850개의 아미노산 폴리펩타이드로 구성되어 있다. 또한, pHem1 유전자는 이미 밟혀진 다른 유전자들과 상동성을 보이지 않았다. 본 연구는 P. intermedia에서의 porphyrin생리 및 hemin 획득기전을 분자생물학적으로 규명하는데 있어 중요한 의의가 있으리라 사료된다. 향후 pHem1 유전자의 특성 분석 등이 수행되어야 할... -
Prevalence, toxin gene profile, antibiotic resistance, and molecular characterization of Clostridium perfringens from diarrheic and non-diarrheic dogs in Korea
Jung-Whan Chon, Kun-Ho Seo, Dongryeoul Bae, Ji-Hee Park, Saeed Khan, Kidon Sung 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 6 368-374 (7 pages)
Clostridium perfringens causes diarrhea and other diseases in animals and humans. We investigated the prevalence, toxin gene profiles, and antibiotic resistance of C. perfringens isolated from diarrheic dogs (DD) and non-diarrheic dogs (ND) in two animal hospitals in Seoul, Korea. Fecal samples were collected from clinically DD (n = 49) and ND (n = 34). C. perfringens was isolated from 31 of 49 DD (63.3%) and 21 of 34 ND dogs (61.8%). All C. perfringens strains were positive for the ... -
A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer
Qing Wang, Mianmian Chen, Wei Zhang 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 12 260-270 (11 pages)
Similar to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682 upregulated genes were identified in vivo. Among the upregulated genes, a signal transduction... -
Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 9 59-70 (12 pages)
Outer membrane proteins (OMPs) of Gram-negative bacteria constitute the first line of defense protecting cells against environmental stresses including chemical, biophysical, and biological attacks. Although the 43-kDa OMP (OMP43) is major porin protein among Bartonella henselae-derived OMPs, its function remains unreported. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate OMP43 function. Interestingly, Δomp43 exhibited weaker proliferative ability than... -
Effect of canine mesenchymal stromal cells overexpressing heme oxygenase-1 in spinal cord injury
Seung Hoon Lee, Yongsun Kim, Daeun Rhew, Ahyoung Kim, Kwang Rae Jo, Yongseok Yoon, Kyeung Uk Choi, Taeseong Jung, Wan Hee Kim, O 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 15 377-386 (10 pages)
Heme oxygenase-1 (HO-1) is a stress-responsive enzyme that modulates the immune response and oxidative stress associated with spinal cord injury (SCI). This study aimed to investigate neuronal regeneration via transplantation of mesenchymal stromal cells (MSCs) overexpressing HO-1. Canine MSCs overexpressing HO-1 were generated by using a lentivirus packaging protocol. Eight beagle dogs with experimentally-induced SCI were divided into GFP-labeled MSC (MSC-GFP) and HO-1-overexpressing MSC... -
Involvement of Heme Oxygenase-1 in Orexin-A-induced Angiogenesis in Vascular Endothelial Cells
Mi-KyoungKim, Hyun-JooPark, , Su-RyunKim, , YoonKyungChoi, Soo-KyungBae, Moon-KyoungBae 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.4 4 327-334 (8 pages)
The cytoprotective enzyme heme oxygenase-1 (HO-1) influences endothelial cell survival, proliferation, inflammatory response, and angiogenesis in response to various angiogenic stimuli. In this study, we investigate the involvement of HO-1 in the angiogenic activity of orexin-A. We showed that orexin-A stimulates expression and activity of HO-1 in human umbilical vein endothelial cells (HUVECs). Furthermore, we showed that inhibition of HO-1 by tin (Sn) protoporphryin-IX (SnPP) reduced... -
Protective Effects of the Nuclear Factor Kappa B Inhibitor Pyrrolidine Dithiocarbamate on Experimental Testicular Torsion and Detorsion Injury
SahinKabay, HilmiOzden, GulGuven, DilekBurukoglu, MehmetCengizUstuner, FatmaTopal, HasanVeysiGunes, DeryaUstuner6, CansuOzbayer 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.4 6 321-326 (6 pages)
Testicular torsion results with the damage of the testis and it is a surgical emergency. Pyrrolidine dithiocarbamate (PDTC) is a low-molecular-weight antioxidant and potent inhibitor of nuclear factor kappa B (NF-κB) activation. In this study, we aimed to investigate the effects of PDTC to testicular torsion-detorsion (T/D) injury. Forty adult male Sprague-Dawley rats were separated into four groups. A sham operation was performed in group I. In group II, torsion is performed 2 hours by 720... -
Heme Oxygenase-1 Induced by Aprotinin Inhibits Vascular Smooth Muscle Cell Proliferation Through Cell Cycle Arrest in Hypertensive Rats
HyoungChulChoi, KwangYounLee, DongHyupLee, YoungJinKang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.4 7 309-313 (5 pages)
Spontaneous hypertensive rats (SHR) are an established model of genetic hypertension. Vascular smooth muscle cells (VSMC) from SHR proliferate faster than those of control rats (Wistar-Kyoto rats; WKY). We tested the hypothesis that induction of heme oxygenase (HO)-1 induced by aprotinin inhibits VSMC proliferation through cell cycle arrest in hypertensive rats. Aprotinin treatment inhibited VSMC proliferation in SHR more than in normotensive rats. These inhibitory effects were associated with... -
Induction of Heme Oxygenase-1 by Traditional Herb Mix Extract Improves MKN-74 Cell Survival and Reduces Stomach Bleeding in Rats by Ethanol and Aspirin in vivo
YoungJinKang, HyungSukMoon, HyeJungKim, HanGeukSeo, JaeHeunLee, KiChurlChang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2007, Vol.11 No.2 4 65-70 (6 pages)
Chinese herb medicines have traditionally been used to treat or alleviate the symptom of various diseases. The rationale for use of certain herbs to certain disorder is now getting unveiled by modern technology. In the present study, we investigated whether herb mix extract (HMX), which is alleged to be useful for gastric ulcer, protects stomach from oxidative stress. Rats were allowed to normal diet with and without HMX (1, 5, 10 mg/kg) for 30 days. To induce gastric ulcer, ethanol (75%, 1.5... -
Effects of Chlorhexidine digluconate on Rotational Rate of n-(9-Anthroyloxy)stearic acid in Model Membranes of Total Lipids Extracted from Porphyromonas gingivalis Outer Membranes
Hye-OckJang, Dong-WonKim, Byeong-IllKim, Hong-GuSim, Young-HoLee, Jong-HwaLee, Jung-HaBae, Moon-KyoungBae, Tae-HyukKwon, IlYun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.2 3 83-88 (6 pages)
The purpose of this study was to provide a basis for studying the molecular mechanism of pharmacological action of chlorhexidine digluconate. Large unilamellar vesicles (OPGTL) were prepared with total lipids extracted from cultured Porphyromonas gingivalis outer membranes (OPG). The anthroyloxy probes were located at a graded series of depths inside a membrane, depending on its substitution position (n) in the aliphatic chain. Fluorescence polarization of n-(9-anthroyloxy)stearic acid was used... -
Effects of Chlorhexidine Digluconate on Rotational Rate of n-(9-Anthroyloxy)stearic Acid in Porphyromonas ginginvalis Outer Membranes
Hye-OckJang, Seong-KweonCha, ChangLee, Min-GakChoi, Sung-RyulHuh, Sang-HunShin, In-KyoChung, IlYun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 2 125-130 (6 pages)
The aim of this study was to provide a basis for studying the molecular mechanism of pharmacological action of chlorhexidine digluconate. Fluorescence polarization of n-(9-anthroyloxy)stearic acid was used to examine the effect of chlorhexidine digluconate on differential rotational mobility of different positions of the number of membrane bilayer phospholipid carbon atoms. The six membrane components differed with respect to 2, 3, 6, 9, 12, and 16-(9-anthroyloxy)stearic acid (2-AS, 3-AS, 6-AS,... -
Electrophysiological Responses of δ -Opioid Receptor Expressed on HEK293 Cells
Kim, Jin-Hyuk, Koh, Young-Ik, Chin, He-Min, Lee, Yong-Sung, Cho, Yeul-Hee, Kim, Kee-Soon 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 2호 12 301-308 (8 pages)
To explore electrophysiological properties of the δ-Opioid receptors artificially expressed in the mammalian cell, effect of an opioid agonist DPDPE (1 μM) on the voltage-sensitive outward currents was examined in the HEK293 (human embryonic kidney) cells transfected with δ-Opioid receptor cDNA cloned from NG-108-15 (neuroblastoma X glioma hybrid) cDNA library. Also studied were effects of 8-bromo-cyclic AMP and naloxone on DPDPE-induced changes in the voltage sensitive outward current. The... -
Effect of Blood Pressure on Contractility of Vascular Smooth Muscle and Endothelium-Dependent Relaxation
Suh, Suk-Hyo, Park, Yee-Tae, Lee, Dong-Chul, Seo, Pil-Won, Kim, Ki-Whan 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 2호 10 279-290 (12 pages)
This study was designed 1) to develop a hypertensive animal model in which the blood pressures (BPs) of symmetric regions (right and left upper extremities) are significantly different and 2) to test the effect of BP per se on the contractility and endothelium-dependent relaxation of vascular smooth muscle. Rabbits were anesthetized with sodium pentobarbital and ventilated with room air via animal respirator. The transverse aorta was exposed through the left second intercostal space and the... -
Synthesis and physicochemical characterization of NixZnx- FeO₂O₄/MWCNT nanostructures as enzyme mimetics with peroxidase-like catalytic activity
Navvabeh Salarizadeh, Minoo Sadri, Hassan Hosseini, Reza. H. Sajedi 한국탄소학회 Carbon Letters 8 Pages
한국탄소학회 Carbon Letters 2017, Vol.24 14 103-110 (8 pages)
Carbon-based magnetic nanostructures in several instances have resulted in improved physicochemical and catalytic properties when compared to multi-wall carbon nanotubes (MWCNTs) and magnetic nanoparticles. In this study, magnetic MWCNTs with a structure of NixZnxFe2O4/MWCNT as peroxidase mimics were fabricated by the one-pot hydrothermal method. The structure, composition and morphology of the nanocomposites were characterized with X-ray diffraction (XRD), Fourier transform infrared... -
Salicylate가 담배 미분화세포 isoperoxidase $A_3$의 활성에 미치는 영향
한국환경생물학회 환경생물 2001, Vol.19 No.3 211-217 (7 pages)
pH 의존적으로 일어났을 뿐만 아니라, noncompetitive inhibition 양상을 나타냈다. 뿐만 아니라 salicylate에 의한 isoperoxidase $A_3$의 활성억제는 $Fe^{2+}$ 첨가에 의하여 거의 완벽하게 보호되었다. Isoeroxidase A3로부터 heme부분을 제거하여 apoperoxidase를 제조한 후 여러가지 금속이온이 apoperoxidase의 활성회복에 미치는 효과를 조사하였다. 그 결과 hemin과 $Fe^{2+}$ 첨가에 의하여 apoperoxidase의 활성이 80% 이상 회복되었으나 $Cu^{2+},;Zn^{2+},;Co^{2+},;Mn^{2+}$ 등은 apoperoxidase의 활성을 재구성시키지... -
철분 강화 식품첨가제용 리포좀의 제조 및 특성
이종우, 전수진 한국식품영양과학회 한국식품영양과학회지 5 Pages
한국식품영양과학회 한국식품영양과학회지 2004, Vol.33 No.5 864-868 (5 pages)
인한 리포좀의 지질산화로 지적되었다 또한, ferrous sulfate에 의한 리포좀의 산화 정도는 hemin의 경우보다 낮은 것으로 관찰되었다. Ferrous sulfate의 자동 산화를 억제하기 위하여 수용성 항산화제인 ascorbic acid가 첨가되었으나, 첨가된 ascorbic acid는 ferrous sulfate와 hemin을 함유한 리포좀의 산화를 억제시키지 못했으며, 오히려 ferrous sulfate에 의한 리포좀의 산화를 촉진시키는 것으로 관찰되었다 여기에 지용성 항산화제인 $alpha$-tocopherol을 추가적으로 첨가함으로써, ferrous sulfate의 자동산화를 억제하고... -
Bacteroides fragilis Roid8의 $alpha-fucosidase$ 조효소 특성
허순영, 박명수, 권빈, 지근억, Heo. Soon Young, Park. Myung Soo, Kwon. Bin, Ji. Geun Eog 한국생물공학회 한국생물공학회지 4 Pages
한국생물공학회 한국생물공학회지 2005, Vol.20 No.5 355-358 (4 pages)
장내에서 가장 우세한 균종에 속하는 것으로서 효소활성을 측정해본 결과 다른 균들에 비해 $alpha-fucosidase$의 활성이 높은 것으로 나타났다. 본 연구실에서 조사된 $alpha-fucosidase$의 생산 최적배지 조성은 1L 배지당 BHI 37g, hemin 10mg, cysteine 0.5g, resazurin 1mg, vitamin K 1mg, starch 5g을 함유하였다. 또한 DEAE-sepharose와 CM-cellulose 크로마토그래피를 거쳐 조효소액을 분리하여 조사한 결과 효소의 최적 반응온도는 $40^{circ}C$, 최적반응 pH는 7.0이었다. 무기 금속이온의 영향은 $Co^{++};and;Zn^{++}$ 존재... -
인간 간암세포주 HepG2에서 heme oxygenase-1 발현에 대한 diallyl disulfide의 효과
김강미, 이상권, 박영철, Kim. Kang-Mi, Lee. Sang-Kwon, Park. Young-Chul 한국생명과학회 생명과학회지 6 Pages
한국생명과학회 생명과학회지 2011, Vol.21 No.7 1046-1051 (6 pages)
HO-1 유도제인 CoPP와 hemin에 의해 자극된 HepG2 세포의 HO-1 발현의 증가를 단백질 수준에서 강력하게 억제시키는 것으로 나타났다. 그러나 DADS는 CoPP에 의한 HO-1 유전자의 mRNA 수준의 전사에는 억제 효과를 보이지 않았으며, 또한 Nrf2와 small Maf의 발현을 증가시키고 핵 내에 축적시키는 것으로 나타났다. 이를 종합해 볼 때 DADS는 단독으로 HO-1 발현을 유도하지 못하고, HO-1 유도제에 의한 HO-1 유전자의 발현과정에서는 전사단계가 아닌 번역단계에서 역할을 함으로써 HO-1의 단백질 합성을 억제하는 것으로 보인다.... -
인간 간암세포주 HepG2에서 NADPH oxidase 활성 억제에 의한 heme oxygenase-1 발현의 조절
이상권, 김강미, 박광훈, 박영철, Lee. Sang-Kwon, Kim. Kang-Mi, Park. Kwang-Hoon, Park. Young-Chul 한국생명과학회 생명과학회지 6 Pages
한국생명과학회 생명과학회지 2011, Vol.21 No.11 1625-1630 (6 pages)
control siRNA를 처리한 세포와 비교할 때 HO-1 발현이 현저히 감소됨을 관찰할 수 있었다. 마지막으로, 세포 내 ROS 생성을 억제하는 GSHmee가 처리된 세포에서는 CoPP나 hemin이 Nrf2의 활성을 증가시키지 못하였고, 그 결과 HO-1의 발현을 유도하지 못하는 것을 알 수 있었는데, 이는 ROS가 CoPP나 hemin에 의한 HO-1 유전자 발현 과정에 중요한 역할을 한다는 것을 의미한다. 이를 종합해 볼 때, 인간 간암세포주 HepG2에서 CoPP나 hemin의 자극에 의한 HO-1 유전자의 발현에는 NADPH oxidase의 활성이 요구된다는 것을 알 수 있고,... -
Coordinated Spatial and Temporal Expression of Voltage-sensitive calcium Channel ${alpha}_{1A}$ and $eta_4$ Subunit mRNAs in Rat Cerebellum
Kim. Dong-Sun-Chin. Hemin, Chin. Hemin-Chin 한국동물학회 Korean journal of biological sciences 6 Pages
한국동물학회 Korean journal of biological sciences 1997, Vol.1 No.4 589-594 (6 pages)
