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Phenotypicand Genotypic Characterization of Methicillin- Resistance Staphylococcus aureus Isolates from Bovine Mastitis for Host Defense Peptide Resistance
Kyoung Mi Kang, Gi Yong Lee, Kun Taek Park, Yong Ho Park, Soo Jin Yang 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 78 226-226 (1 pages)
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Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis
Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)
severe clinical symptoms, including damage to mammary tissues, reduced milk yield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitis treatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel electrophoresis and label-free proteomic approaches. A total... -
In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents
Ayman El Behiry*, Gerd Schlenker, Istvan Szabo, Uwe Roesler 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 6 153-161 (9 pages)
Sensitivity to commercial teat dips (nonoxinol-9 iodine complex and chlorhexidine digluconate) of 56 Staphylococcus (S.) aureus strains isolated from quarter milk samples of various German dairy herds treated with different teat dipping schemes was investigated in this study. The minimum inhibitory concentration was determined using a broth macrodilution method according to the German Veterinary Association guidelines. The main objective of the current study was to induce in vitro resistance... -
Prevalence and antibiotic resistance of mastitis pathogens isolated from dairy herds transitioning to organic management
Young Kyung Park, Lawrence K. Fox*, Dale D. Hancock, Wade McMahan, Yong Ho Park* 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 15 103-105 (3 pages)
and antibiotic resistance of mastitis pathogens isolated from dairies upon conversion from conventional to organic management over a 3-year period was studied. Coagulase-negative staphylococci (CNS) were the most prevalent mastitis pathogens isolated. CNS were significantly less resistant to b-lactam antibiotics when isolated from milk after the herd transitioned to organic management. Cessation of the use of antimicrobial therapies in dairies in combination with organic management could lead to... -
Risk factors associated with bacteriological cure, new infection, and incidence of clinical mastitis after dry cow therapy with three different antibiotics
Yasmin Gundelach, Elke Kalscheuer, Henning Hamann, Martina Hoedemaker* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 5 227-233 (7 pages)
Factors affecting bacteriological cure rates (BCR) and new intramammary infections (IMI) during the dry period as well as clinical mastitis (CM) during early lactation were investigated in 414 German Holstein dairy cows receiving dry cow therapy. Cows were treated with either benethamine benzylpenicillin (300,000 IU), penethamate hydriodide (100,000 IU), and framycetin sulphate (100 mg, n = 136), or cefquinome (150 mg, n = 135), or benzathine cloxacillin (1,280 mg, n = 143). Overall BCR, IMI,... -
A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle
Hidetoshi Higuchi, Hidetomo Iwano, Kazuhiro Kawai, Takehiro Ohta, Tetsu Obayashi, Kazuhiko Hirose, Nobuhiko Ito, Hiroshi Yokota, 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 13 191-194 (4 pages)
A simplified polymerase chain reaction (PCR) assay was developed for fast and easy screening of mycoplasma mastitis in dairy cattle. Species of major mycoplasma strains [Mycoplasma (M.) bovis, M. arginini, M. bovigenitalium, M. californicum, M. bovirhinis, M. alkalescens and M. canadense] in cultured milk samples were detected by this simplified PCR-based method as well as a standard PCR technique. The minimum concentration limit for detecting mycoplasma by the simplified PCR was estimated to be... -
Invasive potential of biofilm-forming Staphylococci bovine subclinical mastitis isolates
Manuela Oliveira*, Ricardo Bexiga, Sandro Filipe Nunes, Cristina Lobo Vilela 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 14 95-97 (3 pages)
a common infectious agent of bovine chronic mastitis, a disease that is difficult to eradicate. The abilities of Staphylococci to be internalized and form a biofilm can contribute to host immunological defence evasion that subsequently impairs antimicrobial therapy. The invasive capability of six S. aureus field isolates with different biofilm- forming profiles was compared in vitro using a bovine mammary epithelial cell line. This was further confirmed in primary cell cultures using fluorescent... -
Effect of mastitis on raw milk compositional quality
Henry Ogola, Anakalo Shitandi, Jackin Nanua 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 6 237-242 (6 pages)
In this study, we investigated the impact of mastitis infection on the quality of milk composition in small-scale dairy bovine herds. The purpose of this study was to find a milk quality somatic cell count (SCC) standard that could be used as an integral component of a control program. In all, 396 quarter milk samples from lactating cross-bred cows (Holstein & Zebu) were analyzed; 56% of these quarters were experiencing intramammary infection, with an overall mean SCC of 5.46 × 105 ± 2.30 ×... -
PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases
Dewanand Rajaram Kalorey, Yuvaraj Shanmugam, Nitin Vasantrao Kurkure, Kapil Kamalakarrao Chousalkar, Sukhadeo Baliram Barbuddhe 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 8 151-154 (4 pages)
present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (coa), clumping factor (clfA), thermonuclease (nuc), enterotoxin A... -
Studies on mastitis, milk quality and health risks associated with consumption of milk from pastoral herds in Dodoma and Morogoro regions, Tanzania
Esron D. Karimuribo, Lughano J. Kusiluka, Robinson H. Mdegela, Angolwisye M. Kapaga, Calvin Sindato, Dominic M. Kambarage 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 7 213-221 (9 pages)
clinical signs, microbiology and California mastitis test (CMT), while milk quality was evaluated using total viable count (TVC)and total coliform count (TCC). Animals were tested for tuberculosis using a single comparative intradermal tuberculin test. The prevalence of subclinical mastitis based on CMT was low (8.3%). The major isolates were Staphylococcus aureus (35.3%), other staphylococci (20.8%), coliforms (27.7%), microcci (5.8%) and streptococci (9.8%). The average TVC of milk in Dodoma... -
Comparative studies on pheno- and genotypic properties of Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany
Siti Isrina Oktavia Salasia, Zaini Khusnan, Christoph Lammler, Michael Zschock 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 3 103-109 (7 pages)
in Central Java, Indonesia and rare among the strains isolated in Hesse, Germany. It was of interest that most of the S. aureus isolated in Central Java, Indonesia harboured the gene cap5 and most of the strains isolated in Hesse, Germany the gene cap8. The phenotypic and genotypic results of the present study might help to understand the distribution of prevalent S. aureus clones among bovine mastitis isolates of both countries and might help to control S. aureus infections in dairy herds. -
Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows
Yong Ho Park, Yi Seok Joo, Joo Youn Park, Jin San Moon, So Hyun Kim, Nam Hoon Kwon, Jong Sam Ahn, William C. Davis and Christoph 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 5 29-39 (11 pages)
Bovine mastitis is an infectious disease with a major economic influence on the dairy industry worldwide. Many factors such as environment, pathogen, and host affect susceptibility or resistance of an individual cow to bovine mastitis. Recently, there has been considerable interest in defining genetic and immunological markers that could be used to select for improved disease resistance. In this study we have analyzed the lymphocyte subpopulations of mastitis-resistant and susceptible cows using... -
Assessment of the California mastitis test usage in smallholder dairy herds and risk of violative antimicrobial residues
Anakalo Shitandi and Gathoni Kihumbu 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 2 5-9 (5 pages)
This study evaluated how predictive the California Mastitis Test (CMT) is for sub-clinical mastitis under tropical smallholder dairy production conditions in Kenya. It intended to establish whether the CMT usage could be contributing to misdiagnosis and consequent mistreatment with animal drugs resulting in residue problems. Milk samples (n = 239) were aseptically collected from lactating cows in the Rift Valley of Kenya and tested using the CMT, somatic cell counts (SCC) and bacterial culture.... -
Identification and epidemiological characterization of Streptococcus uberis isolated from bovine mastitis using conventional and molecular methods
I. U. Khan, A. A. Hassan, A. Abdulmawjood, C. Lammler, W. Wolter, M. Zschock 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 3 213-223 (11 pages)
In the present study 130 S. uberis strains and one S. parauberis strain isolated from bovine milk samples of 58 different farms of various locations in Hesse, Germany, as well as two reference strains of each species were comparatively investigated for cultural, biochemical, serological and molecular properties. All S. uberis strains produced the enzyme β-D-glucuronidase, while the S. parauberis strains were negative. The S. uberis and S. parauberis 16S rRNA genes were amplified by polymerase... -
Capsular polysaccharide typing of domestic mastitis-causing Staphylococcus aureus strains and its potential exploration of bovine mastitis vaccine developmen. I. capsular olysaccharide typing, isolation and purification of the strains
Hong-Ryul Han, Son-Il Pak, Seung-Won Kang, Woo-Seog Jong, Cheol-Jong Youn 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 8 53-60 (8 pages)
One hundred seven isolates of Staphylococcus aureus from bovine mastitis were investigated for colony morphology in serum-soft agar (SSA), autoagglutination in salt, and capsular serotype. Capsular polysaccharide (CP) was purified and quantified from the extracts of clinical isolates. Overall, 89 isolates (83.2%) were diffuse in the SSA, without any difference in the proportion of diffuse colony between type 5 and type 8 strains. Some strains exhibited compact colonies in the SSA and expressed... -
Analysis of antibiotic residues in milk from healthy dairy cows treated with bovine mastitis ointment using ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry
Eun-kee Park , Yong-jae Ryu , Chun-nam Cha , Chang-yeul Yoo , Suk Kim , Hu-jang Lee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2016, 제 56권 제 4호 6 233-239 (7 pages)
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Original Article : Therapeutic effect of oregano essential oil on subclinical bovine mastitis caused by Staphylococcus aureus and Escherichia coli
Byung Wook Cho , Chun Nam Cha , Soo Mi Lee , Mee Jeong Kim , Ju Yeon Park , Chang Yeol Yoo , Song Ee Son , Suk Kim , Hu 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2015, 제 55권 제 4호 7 253-257 (5 pages)
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Classification of acute clinical mastitis on the base of vital signs and complete blood count test in dairy cows
Tai Young Hur , Seog Jin Kang , Young Hun Jung , Hyun June Lee , Kwang Seok Ki , Chang Yong Choe , Guk Hyun Suh 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2013, 제 53권 제 1호 4 19-23 (5 pages)
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Cytokine expression pattern in milk somatic cells of subclinical mastitis-affected cattle analyzed by real time PCR
Vaibhav D Bhatt , Prasad S Khade , Sagar B Tarate , Ajai K Tripathi , Dev S Nauriyal , Dharamshi N Rank , Anju P Kunjadia 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2012, 제 52권 제 4호 3 231-238 (8 pages)
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Clinics : Autogenous toxoid - bacterin treatment for Staphylococcus aureus subclinical mastitis in lactating cows
HwangCheolYong, PakSonIl, HanHongRyul 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 1999, 제 39권 제 4호 25 846-853 (8 pages)
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Patterns on the Outbreaks of Bovine Mastitis and Susceptibility to Antimicrobias of Isolated Causative Agents in a Municipal Area
Jung. Hee-Kon 한국환경보건학회 한국환경위생학회지 12 Pages
한국환경보건학회 한국환경위생학회지 1995, Vol.21 No.2 42-53 (12 pages)
광주시 지역에서 사육하고 있는 젖소 1,614두중 유방염으로 의심되는 730분방 중에서 170분방을 검사분석하여 유방률, 균의 분리와 간이검사법과의 관계, 계절별 분리균의 분포, 항균 요법제에 대한 감수성 등을 검사하였다. 본 원인균은 730분방 중 134분방(18.4%)에서 분리되었으며 체세포 숫자는 평균 $1.620 imes 10^6pm 1.167 imes 10^6/ml$(C.V. 72.0%)이었다. CMT 반응치는 평균 $2.9pm 1.2$(C.V. 41.4%)이었으며 WT 반응치는 평균 $2.8pm 1.2$(C.V. 42.9%)이었다. RBVT와 CMT의 상관계수는 0.82(P<0.001)이었고 RBVT와 WT의... -
Antibacterial Activity of the Honey Bee Venom against Bacterial Mastitis Pathogens Infecting Dairy Cows
Han. Sang-Mi, Lee. Kwang-Gill, Yeo. Joo-Hong, Kweon. Hae-Yong, Kim. Bong-Soon, Kim. Jae-Myung, Baek. Ha-Ju, Kim. Soon-Tae 한국잠사학회 International journal of industrial entomology 6 Pages
한국잠사학회 International journal of industrial entomology 2007, Vol.14 No.2 137-142 (6 pages)
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Morphological Changes Associated with the Antibacterial Action of Silver Ions against Bovine Mastitis Pathogens
강석진, 설재원, 허태영, 정영훈, 최창용, 박상열, Kang. Seog-Jin, Seol. Jae-Won, Hur. Tai-Young, Jung. Young-Hun, Choe. Chang-Yong, Park. Sang-Youel 한국임상수의학회 Journal of veterinary clinics 5 Pages
한국임상수의학회 Journal of veterinary clinics 2011, Vol.28 No.6 576-580 (5 pages)
은 이온(silver ion)은 동물세포에 낮은 독성을 가지면서도 다양한 종류의 세균들에 대해 강력한 항균력을 가지고 있다. 본 연구에서는 젖소 유방염 원인균들에 대한 은 이온의 항균효과를 전자현미경(TEM)을 통해 조사하였다. 본 실험에 사용된 균주는, 젖소 유방염의 주요 원인균인 Escherichia coli 와 Staphylococcus aureus를 사용하였다. 유방염 원인균에 대한 은 이온의 항균효과를 관찰하기 위해 50 ${mu}g/mL$의 은 이온을 2시간 동안 세균에 노출시킨 후 전자현미경(Energy-Filtering Transmission Electron Microscopy, EFTEM)...


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