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Analysis for nucleotide sequence of the small membrane (sM) protein gene of porcine epidemic diarrhea virus Chinju99 isolated in Korea
SangGeonYeo, ChangHeeLee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2007, 제 47권 제 2호 5 169-174 (6 pages)
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Thiol-dependent Redox Mechanisms in the Modification of ATP-Sensitive Potassium Channels in Rabbit Ventricular Myocytes
JinHan, NariKim, DangVanCuong, ChunghuiKim, EuiyongKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.1 4 15-23 (9 pages)
this study, the properties of thiol redox modulation of the ATP-sensitive K (KATP) channel were examined in rabbit ventricular myocytes. Rabbit ventricular myocytes were isolated using a Langendorff column for coronary perfusion and collagenase. Single-channel currents were measured in excised membrane patch configuration of patch-clamp technique. The thiol oxidizing agent 5,5'-dithio-bis-(2-nitro-benzoic acid) (DTNB) inhibited the channel activity, and the inhibitory effect of DTNB was... -
The Characterization of the Increase of Membrane Conductance after Depolarization in Single Rat Adrenal Chromaffin Cells
WonilLim, SangJeongKim, JunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.1 12 95-100 (6 pages)
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Molecular Association of Glucose Transporter in the Plasma Membrane of Rat Adipocyte
Hah, Jong-Sik 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 2 115-124 (10 pages)
Molecular association of glucose transporters with the other proteins in the plasma membrane was assessed by gel electrophoresis and immunoblot techniques. Approximately 31.5±5.1% of GLUT-4, 64.8±2.7% of clathrin, 48.7% of total protein in the plasma membrane (PM) were found insoluble upon extraction with 1% Tx-100. Sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed that the Tx-100 insoluble PM fraction contained about 4 major polypeptides with apparent molecular weight of... -
Effect of Two-step Surface Modification of ACtivated CARbon on the Adsorption Characteristics of Metak Ions in Wastewater I.Equilibrium and Batch Adsorptions
Geun Il Park, Jae Kwang Lee, Seung Kon Ryu, Joon Hyung Kim 한국탄소학회 Carbon Letters 7 Pages
한국탄소학회 Carbon Letters 2002, Vol.3 No.4 6 219-225 (7 pages)
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Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 9 59-70 (12 pages)
Outer membrane proteins (OMPs) of Gram-negative bacteria constitute the first line of defense protecting cells against environmental stresses including chemical, biophysical, and biological attacks. Although the 43-kDa OMP (OMP43) is major porin protein among Bartonella henselae-derived OMPs, its function remains unreported. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate OMP43 function. Interestingly, Δomp43 exhibited weaker proliferative ability than... -
Paclitaxel inhibits the hyper-activation of spleen cells by lipopolysaccharide and induces cell death
Hyun-Ji Kim, Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 3 453-458 (6 pages)
Paclitaxel was isolated from the bark of the Pacific yew, Taxus brevifolia, and used as an anticancer agent. Paclitaxel prevents cancer cell division by inhibiting spindle fiber function, inducing cell death. A recent study demonstrated that paclitaxel binds to myeloid differentiation protein-2 of Toll-like receptor 4 and prevents the signal transduction of lipopolysaccharide (LPS). Paclitaxel converts immune cells hypo-responsive to LPS. In this study, we investigated whether paclitaxel can... -
The current epidemiological status of infectious coryza and efficacy of PoulShot Coryza in specific pathogen-free chickens
Moo-Sung Han, Jong-Nyeo Kim, Eun-Ok Jeon, Hae-Rim Lee, Bon-Sang Koo, Kyeong-Cheol Min, Seung-Baek Lee, Yeon-Ji Bae, Jong-Suk Mo, 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 8 323-330 (8 pages)
A serological survey was conducted to evaluate the number of flocks that were positive for Av. paragallinarum by utilizing a HI test to determine the existence of serovar A. Serological surveys revealed high positivity rates of 86.4% in 2009, 78.9% in 2010, 70.0% in 2011, and 69.6% in 2012. We also challenged specific pathogen-free chickens with isolated domestic strains, ADL121286 and ADL121500, according to the measured efficacy of the commercial IC vaccine, PoulShot Coryza. We confirmed the... -
Time-course changes of hippocalcin expression in the mouse hippocampus following pilocarpine-induced status epilepticus
Hee-Soo Choi, Choong-Hyun Lee* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 2 137-144 (8 pages)
Hippocalcin participates in the maintenance of neuronal calcium homeostasis. In the present study, we examined the time-course changes of neuronal degeneration and hippocalcin protein level in the mouse hippocampus following pilocarpine-induced status epilepticus (SE). Marked neuronal degeneration was observed in the hippocampus after SE in a time-dependent manner, although neuronal degeneration differed according to the hippocampal subregions. Almost no hippocalcin immunoreactivity was detected... -
Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis
Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)
involved with binding and structural molecule activities. Vimentin and ㅁㅁα-enolase were central “functional hubs” in the network. Based on results from the present study, disease-induced alterations of protein expression in mammary glands and potential markers for the effective treatment of E. coli mastitis were identified. These data have also helped elucidate defense mechanisms that protect the mammary glands and promote the pathogenesis of E. coli mastitis. -
Treadmill exercise prevents diabetes-induced increases in lipid peroxidation and decreases in Cu,Zn-superoxide dismutase levels in the hippocampus of Zucker diabetic fatty rats
Jong Whi Kim, Junghyun Chae , Sung Min Nam, Yo Na Kim, Dae Young Yoo, Jung Hoon Choi, Hyo Young Jung, Wook Song, In Koo Hwang, J 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 2 11-16 (6 pages)
the hippocampus of the ZDF rats were significantly higher than those of the ZLC rats whereas SOD1 levels in the hippocampus of the ZDF rats were moderately decreased. Notably, treadmill exercise prevented the increase of blood glucose levels in ZDF rats. In addition, treadmill exercise significantly ameliorated changes in MDA and SOD1 levels in the hippocampus although SOD activity was not altered. These findings suggest that diabetes increases lipid peroxidation and... -
The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention
Do-Geun Kim, Chang-Seon Song, In-Soo Choi, Seung-Yong Park, Joong-Bok Lee, Sang-Soo Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 10 307-313 (7 pages)
The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained in the endoplasmic reticulum (ER) rather than transported to the plasma membrane of the cell. In this study, we performed confocal laser scanning microscopy analysis of variants of GP3 and foundthat the signal sequence of the GP3 led to confinement of GP3 in the ER, while the functional ortransmembrane... -
Expression and regulation of Enpp2 in rat uterus during the estrous cycle
Hyo-Jin Ahn, Hyun Yang, Beum-Soo An, Kyung-Chul Choi, Eui-Bae Jeung* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 11 379-385 (7 pages)
16. Interestingly, the expression of Enpp2 mRNA and protein were down-regulated by E2 in the uterus during estrus but not during proestrus or diestrus, suggesting that Enpp2 may play a role in uterine function during estrus. Enpp2 is primarily localized in the stromal cells of the endometrium during proestrus and estrus. During diestrus, Enpp2 was highly expressed in the epithelial cells of the endometrium. Taken together, these results suggest that uterine Enpp2 may be regulated by E2 and plays... -
Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl3-induced toxicity in the rat forebrain cortex
Ivana D. Stevanović*, Marina D. Jovanović, Ankica Jelenković, Miodrag Čolić, Ivana Stojanović, Mil 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 3 15-22 (8 pages)
The present experiments were done to determine the effectiveness of a non-specific nitric oxide synthase inhibitor, N-nitro-L-arginine methyl ester (L-NAME), on oxidative stress parameters induced by aluminium chloride (AlCl3) intrahippocampal injections in Wistar rats. Animals were sacrificed 3 h and 30 d after treatments, heads were immediately frozen in liquid nitrogen and forebrain cortices were removed. Crude mitochondrial fraction preparations of forebrain cortices were used for the... -
Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos
Kilyoung Song, Eunsong Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 12 81-87 (7 pages)
for 42 h than in other groups. Supplementation of a defined maturation medium with 100 μM CYS or 100 μM β-ME showed no stimulatory effect on oocyte maturation, embryo cleavage, or blastocyst formation after PA. β-ME treatment during IVM decreased embryo cleavage after SCNT compared to pFF or PVA treatments, but no significant difference was found in blastocyst formation (7-16%) among the four treatment groups. The results indicated that maturation of oocytes for 42 h was beneficial for the... -
Histology of two rice bodies isolated from the stifle of an adult draught horse stallion
Nicole Schneider, Marianne Heimann , Jean-Philippe Lejeune , Denis R.V.G.Verwilghen, Ginette P.Deby-Dupont, Didier A.Serteyn 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 15 83-85 (3 pages)
In the human and equine species, different kinds of free floating intra-articular particles are related to certain disorders. Osteochondral fragments formed during osteochondrosis dissecans are the most common finding in the equine species, whereas in humans rice bodies due to rheumatoid arthritis are more frequent.Herein we report a third type of floating body inside the stifle of an adult draught horse stallion, in macroscopic appearance similar to articular rice bodies known in humans.As... -
Characterization of the Recombinant Proteins of Porcine ircovirus Type 2 Field Isolate Expressed in the Baculovirus System
Yu Na Kim, Jin Hyun Kim, Kyoung Soo Kang, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 4 19-23 (5 pages)
to the target genes of the PCV type 2. Cloned genes were inserted into the baculovirus expression vector and PCV recombinant proteins were expressed using baculovirus expression system. Recombinant protein expression was determined by indirect immunofluorescent assay (IFA) and immunoblotting using polyclonal antiserum to PCV. ORF1 gene expressed two proteins with approximately 17 kDa and 31 kDa proteins in the baculovirus system. Recombinant protein of the ORF2 was similar to that of the native... -
Expression and characterization of the flavoprotein domain of gp91phox
Chang-Hoon Han and Mun-Han Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 3 19-26 (8 pages)
a 1:1 molar ratio of FAD to TRX-gp91(phox) (306- 569), and showed the same Km for NADPH as that for superoxide generating activity by the intact enzyme. Activity was not inhibited by superoxide dismutase, indicating that it was not mediated by superoxide, but was blocked by an inhibitor of the respiratory burst oxidase, diphenylene iodonium (DPI). In the presence of Rac1, the cytosolic regulatory protein p67(phox) stimulated the NBT reductase activity, but p47(phox) had no effect. Truncated... -
Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency
Mi-ja Park , Seong-joon Joh , Kang-seuk Choi , Aeran Kim , Min-goo Seo , Jae-young Song , Seon-jong Yun 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 4 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2016, 제 56권 제 3호 9 189-192 (4 pages)
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Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV)in transgenic potato and its immunogenicity in mice
Dong Joo Ahn , Jung Won Youm , Suk Weon Kim , Won Kee Yoon , Hyoung Chin Kim , Tai Young Hur , Young Hee Joung , Jae Heu 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2013, 제 53권 제 4호 5 217-224 (8 pages)
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EFFECTS OF SODIUM NITROPRUSSIDE ON THE FORMATION AND ACTIVATION OF THE OSTEOCLAST IN CULTURE
Young-Jae Yoo, Jung-Kun Kim, Kyung-Suk Cha 대한치과교정학회 The Korean Journal of Orthodontics 10 Pages
대한치과교정학회 The Korean Journal of Orthodontics 1995, 제 25권 제 6호 6 705-714 (10 pages)
dentin slices, treatment of 300µM SNP resulted in a significant decrease in dentin resorption by osteoclasts in terms of total resorption area and average individual area. We also examined the effect of SNP on formation of osteoclast-like cells that is TRAP-positive multinucleated cells from chicken and rat bone marrow cells in the presence or absence of 10-8M l,25[OH]2D3. The addition of 300 µM SNP inhibited the formation of TRAP-positive multinucleated cells. The present data suggest... -
Epigenetic modification of α-N-acetylgalactosaminidase enhances cisplatin resistance in ovarian cancer
Ye-NaHa, HyeYounSung, San-DukYang, YunJuChae, WoongJu, Jung-HyuckAhn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.1 5 43-51 (9 pages)
Although cisplatin is one of the most effective antitumor drugs for ovarian cancer, the emergence of chemoresistance to cisplatin in over 80% of initially responsive patients is a major barrier to successful therapy. The precise mechanisms underlying the development of cisplatin resistance are not fully understood, but alteration of DNA methylation associated with aberrant gene silencing may play a role. To identify epigenetically regulated genes directly associated with ovarian cancer cisplatin... -
Participation of COX-1 and COX-2 in the contractile effect of phenylephrine in prepubescent and old rats
GustavoGuevara-Balcazar, IsraelRamirez-Sanchez, ElviaMera-Jimenez, IvanRubio-Gayosso, MariaEugeniaAguilar-Najera, MariaC.Castillo-Her 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.4 6 407-413 (7 pages)
three distinct ages: prepubescent, young and old adult. The results suggest that there is a higher participation of prostanoids in the contractile effect of phenylephrine in pre-pubescent rats, and a lower participation of the same in old rats. Contrarily, there seems to be a higher participation of prostanoids in the contractile response of the coronary artery of older than pre-pubescent rats. Considering that the changes in the expression of COX-2 were similar for the three age groups and the... -
Potentiation of decursinol angelate on pentobarbital-induced sleeping behaviors via the activation of GABAA-ergic systems in rodents
JaeHoonWoo, Tae-WooHa, Jae-SeonKang, JinTaeHong, Ki-WanOh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.1 4 27-36 (10 pages)
dosage. DCA (50 mg/kg, p.o.) itself modulated sleep architectures; DCA reduced the counts of sleep/wake cycles. At the same time, DCA increased total sleep time, but not non-rapid eye movement (NREM) and rapid eye movement (REM) sleep. In the molecular experiments. DCA (0.001, 0.01 and 0.1 μg/ml) increased intracellular Cl- influx level in hypothalamic primary cultured neuronal cells of rats. In addition, DCA increased the protein expression of glutamic acid decarboxylase (GAD65/67) and GABAA...
