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세포외 분비시 막 캐패시턴스를 측정하기 위한 위상감지법(phase detector technique)의 이론적 분석.
Cha. Eun-Jong, Goo. Yong-Sook, Lee. Tae-Soo 한국의학물리학회 의학물리 15 Pages
한국의학물리학회 의학물리 1992, Vol.3 No.2 43-57 (15 pages)
위상감지법(phase detector technique)은 세포의 막 캐패시턴스(membrane capacitance)를 실시간적으로 측정할 수 있는 유일한 방법이나 측정이 행해지는 동안 세포의 상태가 끊임없이 변화하기 때문에 피할 수 없는 측정오차가 존재한다. 본 연구는 이 오차의 근원을 분석하여 위상감지법의 실용한계를 규정하고자 하였다. 이론적 분석에 기초하여 다음과 같은 사실을 밝힐 수 있었다. 1) access conductance와 membrane conductance의 변화에 기인하는 측정오차를 줄이기 위해서는 초기 위상치를 올바로 선택하여야 한다. 2) 이 때... -
Comparison of electrophysiological properties of two types of pre-sympathetic neurons intermingled in the hypothalamic paraventricular nucleus
Yiming Shen, Seong Kyu Han, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 2 483-491 (9 pages)
using patch-clamp and retrograde-tracing techniques. Most neurons (＞70%) in both groups spontaneously fired in the cell-attached mode. When compared to the PVNIML neurons, the PVNRVLM neurons had a lower firing rate and a more irregular firing pattern (p ＜ 0.05). The PVNRVLM neurons showed smaller resting membrane potential, slower rise and decay times, and greater duration of spontaneous action potentials (p ＜ 0.05). The PVNRVLM neurons received greater inhibitory synaptic inputs (frequency,... -
Effects of acidic pH on voltage-gated ion channels in rat trigeminal mesencephalic nucleus neurons
Jin-EonHan, Jin-HwaCho, In-SunChoi, Do-YeonKim, Il-SungJang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.2 9 215-223 (9 pages)
whole-cell patch clamp technique on mechanically isolated rat mesencephalic trigeminal nucleus neurons. The application of a pH 6.5 solution had no effect on the peak amplitude of voltage-dependent K+ currents. A pH 6.0 solution slightly, but significantly inhibited the peak amplitude of voltage-dependent K+ currents. The pH 6.0 also shifted both the current-voltage and conductance-voltage relationships to the depolarization range. The application of a pH 6.5 solution scarcely affected the peak... -
Myometrial relaxation of mice via expression of two pore domain acid sensitive K+ (TASK-2) channels
Kyu-SangKyeong, SeungHwaHong, YoungChulKim, *, WoongChoi, SunChulMyung, MooYeolLee, RaYoungYou, ChanHyungKim, SoYeonKwon, HikaruSuzuki, Yeo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.5 13 547-556 (10 pages)
in marine myometrium. Isometric contraction measurements and patch clamp technique were used to verify TASK conductance in murine myometrium. Western blot and immunehistochemical study under confocal microscopy were used to investigate molecular identity of TASK channel. In this study, we showed that TEA and 4-AP insensitive non‐inactivating outward K+ current (NIOK) may be responsible for the quiescence of murine pregnant longitudinal myometrium. The characteristics of NIOK coincided with... -
Low Non-NMDA Receptor Current Density as Possible Protection Mechanism from Neurotoxicity of Circulating Glutamate on Subfornical Organ Neurons in Rats
WoneeChong, SeongNamKim, SeongKyuHan, SoYeongLee, PanDongRyu 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.2 13 177-181 (5 pages)
it remains unclear how SFO neurons are protected from excitotoxicity caused by circulating glutamate. In this study, we compared the glutamate-induced whole cell currents in SFO neurons to those in hippocampal CA1 neurons using the patch clamp technique in brain slice. Glutamate (100 μM) induced an inward current in both SFO and hippocampal CA1 neurons. The density of glutamate-induced current in SFO neurons was significantly smaller than that in hippocampal CA1 neurons (0.55 vs. 2.07 pA/pF,... -
Long-Term Potentiation of Excitatory Synaptic Strength in Spinothalamic Tract Neurons of the Rat Spinal Cord
SungWonHur, JooMinPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.6 12 553-558 (6 pages)
to the thalamus. Optical technique using fluorescent dye has made it possible to identify the STT neurons in the spinal cord. Evoked fast mono-synaptic, excitatory postsynaptic currents (eEPSCs) were measured in the STT neurons. Time-based tetanic stimulation (TBS) was employed to induce long-term potentiation (LTP) in the STT neurons. Coincident stimulation of both pre- and postsynaptic neurons using TBS showed immediate and persistent increase in AMPA receptor-mediated EPSCs. LTP can also be... -
Disappearance of Hypoxic Pulmonary Vasoconstriction and O2-Sen-sitive Nonselective Cationic Current in Arterial Myocytes of Rats Under Ambient Hypoxia
HaeYoungYoo, SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.5 13 463-468 (6 pages)
Acute hypoxia induces contraction of pulmonary artery (PA) to protect ventilation/perfusion mismatch in lungs. As for the cellular mechanism of hypoxic pulmonary vasoconstriction (HPV), hypoxic inhibition of voltage-gated K+ channel (Kv) in PA smooth muscle cell (PASMC) has been suggested. In addition, our recent study showed that thromboxane A2 (TXA2) and hypoxia-activated nonselective cation channel (INSC) is also essential for HPV. However, it is not well understood whether HPV is maintained... -
5-Hydroxytryptamine Generates Tonic Inward Currents on Pacemaker Activity of Interstitial Cells of Cajal from Mouse Small Intestine
PawanKumarShahi, SeokChoi, DongChuanZuo, CheolHoYeum, PyungJinYoon, JunLee, YoungDaeKim, ChanGukPark, ManYooKim, HyeRangShin, HyunJungOh, J 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.3 2 129-135 (7 pages)
whole-cell patch-clamp technique, intracellular Ca2＋ ([Ca2＋]i) analysis, and RT-PCR in ICC. Exogenously-treated 5-HT showed tonic inward currents on pacemaker currents in ICC under the voltage-clamp mode in a dose-dependent manner. Based on RT-PCR results, we found the existence of 5-HT2B, 3, 4, and 7 receptors in ICC. However, SDZ 205557 (a 5-HT4 receptor antagonist), SB 269970 (a 5-HT7 receptor antagonist), 3-tropanylindole − 3 − carboxylate methiodide (3-TCM; a 5-HT3 antagonist)... -
Octyl Gallate Inhibits ATP-induced Intracellular Calcium Increase in PC12 Cells by Inhibiting Multiple Pathways
YujieGuo, YiJaeHong, Hyun-JongJang, Myung-JunKim, Duck-JooRhie, Yang-HyeokJo, SangJuneHahn, ShinHeeYoon 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.1 4 21-28 (8 pages)
Phenolic compounds affect intracellular free Ca2＋ concentration ([Ca2＋]i) signaling. The study examined whether the simple phenolic compound octyl gallate affects ATP-induced Ca2＋ signaling in PC12 cells using fura-2-based digital Ca2＋ imaging and whole-cell patch clamping. Treatment with ATP (100</SUP>ՌM) for 90 s induced increases in [Ca2＋]i in PC12 cells. Pretreatment with octyl gallate (100 nM to 20ՌM) for 10 min inhibited the ATP-induced [Ca2＋]i response in a... -
Multiple Residues in the P-Region and M2 of Murine Kir 2.1 Regulate Blockage by External Ba2＋
YoungMeeLee, GarethA.Thompson, IanAshmole, MarkLeyl, InsukSo, PeterR.Stanfield 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.1 10 61-69 (9 pages)
Kir 2.1. We expressed mutant and wild type murine Kir 2.1 in Chinese hamster ovary (CHO) cells and used the whole cell patch-clamp technique to record K＋ currents in the absence and presence of externally applied Ba2＋. Wild type Kir2.1 was blocked by externally applied Ba2＋ in a voltage and concentration dependent manner. Mutants of Y145 in the selectivity filter showed little change in the kinetics of Ba2＋ blockage. The estimated Kd(0) was 108ՌM for Kir2.1 wild type, 124ՌM for a... -
Inhibition of Pacemaker Activity of Interstitial Cells of Cajal by Hydrogen Peroxide via Activating ATP-sensitive K+ Channels
SeokChoi, ShankarPrasadParajuli, HyeonSookCheong, DilliParasadPaudyal, CheolHoYeum, PyungJinYoon, JaeYeoulJun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2007, Vol.11 No.1 3 15-20 (6 pages)
whole cell patch technique at 30oC, ICC generated spontaneous pacemaker potential under current clamp mode (I=0) and inward currents (pacemaker currents) under voltage clamp mode at a holding potential of 70 mV. When ICC were treated with H2O2 in ICC, H2O2 hyperpolarized the membrane potential under currents clamp mode and decreased both the frequency and amplitude of pacemaker currents and increased the resting currents in outward direction under voltage clamp mode. Also, H2O2 inhibited... -
Protein Kinase C Activates ATP-sensitive Potassium Channels in Rabbit Ventricular Myocytes
NariKim, JaeBoumYoum, HyunJoo, HyungkyuKim, EuiyongKim, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.4 1 187-194 (8 pages)
C (PKC) modulated the activity of KATP channels by recording KATP channel currents in rabbit ventricular myocytes using patch-clamp technique and found that phorbol 12,13-didecanoate (PDD) enhanced pinacidil-induced KATP channel activity in the cell-attached configuration; and this effect was prevented by bisindolylmaleimide (BIM). KATP channel activity was not increased by 4α-PDD. In excised inside- out patches, PKC stimulated KATP channels in the presence of 1 mM ATP, and this effect was... -
PGE2 Regulates Pacemaker Currents through EP2-Receptor in Cultured Interstitial Cells of Cajal from Murine Small Intestine
SeokChoi, KyungWonCho, Jong-HyunReu, Jun-SooKim, HyunSikMun, MyungYoungKim, KwangChulPark, GwangSikHeo, SungJongChang, CheolHoYeum, PyungJ 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.3 6 153-160 (8 pages)
The interstitial cells of Cajal (ICCs) are the pacemaker cells in gastrointestinal tract and generate electrical rhythmicity in gastrointestinal muscles. Therefore, ICC may be modulated by endogenous agents such as neurotransmitter, hormones, and prostaglandins (PGs). In the present study, we investigated the effects of prostaglandins, especially PGE2, on pacemaker currents in cultured ICCs from murine small intestine by using whole-cell patch clamp techniques. ICCs generated spontaneous slow... -
4-Aminopyridine Inhibits the Large-conductance Ca2+-activated K+ Channel (BKCa) Currents in Rabbit Pulmonary Arterial Smooth Muscle Cells
YoungMinBae, AeranKim, BokyungKim, Sung-IlCho, JunghwanKim, YungEEarm 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.1 5 25-28 (4 pages)
of their functional role. In the present study, we tested the hypothesis that 4-aminopyridine (4-AP) could affect the large conductance Ca2-activated K channel (BKCa) currents using perforated-patch or cell-attached configuration of patch-clamp technique in the rabbit pulmonary arterial smooth muscle. Application of 4-AP reversibly inhibited the spontaneous transient outward currents (STOCs). The reversal potential and the sensitivity to charybdotoxin indicated that the STOCs were... -
Thiol-dependent Redox Mechanisms in the Modification of ATP-Sensitive Potassium Channels in Rabbit Ventricular Myocytes
JinHan, NariKim, DangVanCuong, ChunghuiKim, EuiyongKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.1 4 15-23 (9 pages)
a Langendorff column for coronary perfusion and collagenase. Single-channel currents were measured in excised membrane patch configuration of patch-clamp technique. The thiol oxidizing agent 5,5'-dithio-bis-(2-nitro-benzoic acid) (DTNB) inhibited the channel activity, and the inhibitory effect of DTNB was reversed by dithiothreitol (disulfide reducing agent; DTT). DTT itself did not have any effect on the channel activity. However, in the patches excised from the metabolically compromised cells,... -
Identification of ATP-sensitive K+ Conductances in Male Rat Major Pelvic Ganglion Neurons
Kyu-SangPark, Seung-KyuCha, Keon-IlLee, JaeYeoulJun, Seong-WooJeong, InDeokKong, JoongWooLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.5 3 247-254 (8 pages)
Major pelvic ganglia (MPG) neurons are classified into sympathetic and parasympathetic neurons according to the electrophysiological properties; membrane capacitance (Cm), expression of T-type Ca2 channels, and the firing patterns during depolarization. In the present study, function and molecular expression of ATP-sensitive K (KATP) channels was investigated in MPG neurons of male rats. Only in parasympathetic MPG neurons showing phasic firing patterns, hyperpolarizing changes... -
Nimodipine as a Potential Pharmacological Tool for Characterizing R-Type Calcium Currents
SeogBaeOh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.6 8 511-519 (9 pages)
Nimopidine, one of dihydropyridine derivatives, has been widely used to pharmacologically identify L-type Ca currents. In this study, it was tested if nimodipine is a selective blocker for L-type Ca currents in sensory neurons and heterologous system. In mouse dorsal root ganglion neurons (DRG), low concentrations of nimodipine (＜10μM), mainly targeting L-type Ca currents, blocked high-voltage-activated calcium channel currents by ∼38%. Interestingly, high concentrations of nimodipine... -
Altered Electrophysiological Properties of Coronary Artery in Isoprenaline-Induced Cardiac Hypertrophy
NariKim, JinHan, EuiyongKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.5 7 413-421 (9 pages)
whole-cell and inside-out patch-clamp technique in single coronary arterial SMCs from rabbits subjected to isoprenaline-induced cardiac hypertrophy. Coronary arterial SMCs underwent significant changes in ultrastructure. The unitary current amplitude and the open-state probability of KCa channel were significantly reduced in hypertrophy without open-time and closed-time kinetic. The concentration-response curve of KCa channel to Ca2 is shifted to the right in hypertrophy. The reduction in... -
Effect of Propofol on Ion Channels in Acutely Dissociated Dorsal Raphe Neuron of Sprague-Dawley Rats
Bong-JaeLee, Moo-IlKwon, Min-ChulShin, Youn-JungKim, Chang-JuKim, SoonAeKim, Ee-HwaKim, Joo-HoChung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.2 10 189-197 (9 pages)
produced by the nicotinic acetylcholine- and glutamate-responsive channels, rat dorsal raphe neurons were acutely dissociated and the nystatin-perforated patch-clamp technique under voltage- clamp conditions was used to observe their responses to the administration of propofol. Propofol evoked ion currents in a dose-dependent manner, and propofol (104 M) was used to elicit ion currents through the activation of GABAA, glycine, nicotinic acetylcholine and glutamate receptors. Propofol at a... -
Activation of ATP-sensitive Potassium Channels by the Predominant Metabolite of Isoflurane in Rabbit Ventricular Myocytes
JinHan, NariKim, EuiyongKim, SungjuKim, KangheeCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.2 7 165-175 (11 pages)
enzymatic dissociation procedure. Patch-clamp techniques were used to record single-channel currents. KATP channel activities were assessed before and after the application of TFA with the inside-out patch mode. Results: TFA enhanced channel activity in a concentration-dependent fashion. The concentration of TFA for half-maximal activation and the Hill coefficient were 0.03 mM and 1.2, respectively. TFA did not affect the single channel conductance of KATP channels. Analysis of open and closed... -
Modulation of L-type Ca2+ Channel Currents by Various Protein Kinase Activators and Inhibitors in Rat Clonal Pituitary GH3 Cell Line
YoungMinBae, Hye-JungBaek, HanaCho, YungEEarm, Won-KyungHo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.2 4 139-146 (8 pages)
hormone secretions are also regulated by various kinds of protein kinases, we investigated the role of some kinase activators and inhibitors in the regulation of the L-type Ca2 channel currents in rat pituitary GH3 cells using the patch-clamp technique. Phorbol 12,13-dibutyrate (PDBu), a protein kinase C (PKC) activator, and vanadate, a protein tyrosine phosphatase (PTP) inhibitor, increased the Ba2 current through the L-type Ca2 channels. In contrast, bisindolylmaleimide I... -
Modulation of Ca2+-Activated Potassium Channels by cGMP- Dependent Signal Transduction Mechanism in Cerebral Arterial Smooth Muscle Cells of the Rabbit
JinHan, NariKim, KwangbokLee, EuiyongKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.6 2 439-444 (6 pages)
of patch-clamp techniques. Both molsidomine derivative 3-morpholinosydnonimine-N-ethylcarbamide (SIN-1, 50μM) and 8-(4-Chlorophenylthio)-guanosine-3',5'-cyclic monophosphate (8-pCPT-cGMP, 100μM), a membrane-permeable analogue of cGMP, increased the KCa channel activity in the cell-attached patch configuration, and the effect was removed upon washout of the drugs. In inside-out patches, single-channel current amplitude was not changed by SIN-1 and 8-pCPT-cGMP. Application of ATP (100μM), cGMP... -
Modulation of ATP-Induced Activation of the Muscarinic K+ Channel Activity by Protein Kinase C
Yang-MiKim, Hong-KiPark, Jae-HeeHan, Choon-OkPark, Seong-GeunHong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.6 11 743-753 (11 pages)
The atrial acetylcholine-activated K (KACh) channel is gated by the pertussis toxin-sensitive inhibitory G (GK) protein. Earlier studies revealed that ATP alone can activate the KACh channel via transphosphorylation mediated by nucleoside-diphosphate kinase (NDPK) in atrial cells of rabbit and guinea pig. This channel can be activated by various agonists and also modulated its function by phosphorylation. ATP-induced KACh channel activation (AIKA) was maintained in the presence of the... -
The Effects of Intracellular Monocarboxylates on the ATP-sensitive Potassium Channels in Rabbit Ventricular Myocytes
NariKim, JinHan, Eui-YongKim, Won-KyungHo, YungE.Earm 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.5 6 581-589 (9 pages)
of monocarboxylates (acetate, formate, lactate, and pyruvate) on KATP channels in isolated rabbit ventricular myocytes. Single channel currents of KATP channels were recorded using the excised inside-out or permeabilized attached (open-cell) patch-clamp technique at room temperature. Intracellular application of acetate, formate and pyruvate led to an inhibition of channel activity, whereas intracellular application of lactate increased channel activity. These effects were reversible upon... -
Modulation of Outward Potassium Currents by Nitric Oxide in Longitudinal Smooth Muscle Cells of Guinea-pig Ileum
Seong-ChunKwon, SeJoongRim, Bok-SoonKang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.2 11 225-232 (8 pages)
currents in nitric oxide-induced relaxation in intestinal smooth muscle, we used whole-cell patch clamp technique in freshly dispersed guinea-pig ileum longitudinal smooth muscle cells. When cells were held at 60 mV and depolarized from 40 mV to 50 mV in 10 mV increments, sustained outward K+ currents were evoked. The outward K+ currents were markedly increased by the addition of 10 ㄍM sodium nitroprusside (SNP). 10 ㄍM S-nitroso-N-acetylpenicillamine (SNAP) and... -
Two Types of Voltage-dependent Outward Potassium Currents in Smooth Muscle Cells of Rabbit Basilar Atery
TongMookKang, InsukSo, DaeYongUhm, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 15 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.2 7 169-183 (15 pages)
current (Ito) with patch-clamp technique in single smooth muscle cells (SMCs) isolated from rabbit basilar artery, and investigated the characteristics of them. The time-courses of activation were well fitted by exponential function raised to second power (n2) in IK(v) and fourth power (n4) in Ito. The activation, inactivation and recovery time courses of Ito were much faster than that of IK(V). The steady-state activation and inactivation of IK(V) was at the more hyperpolarized range than that... -
Effect of pH on Calcium-Activated Potassium Channels in Pulmonary Arterial Smooth Muscle Cells of the Rabbit
Lee, Suk-Ho, Ho, Won-Kyung, Earm, Yung-E 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 1호 2 17-26 (10 pages)
by treatment with collagenase and elastase. Using the patch clamp technique, potassium channel activity was recorded from the inside-out membrane patch. The channel had a sin히e channel conductance of about 360 pS in symmetrical concentration of K on both sides of the patch, 150 mM, and had a linear current-voltage relationship. During the application of 10 mM tetraethylammonium (TEA) to the intracellular membrane surface, the amplitude of single channel current was reduced and very rapid... -
Tricyclic antidepressant amitriptyline inhibits 5-hydroxytryptamine 3 receptor currents in NCB-20 cells
Yong Soo Park, Seok Ho Myeong, In-Beom Kim, Ki-Wug Sung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.5 12 585-595 (11 pages)
Amitriptyline, a tricyclic antidepressant, is commonly used to treat depression and neuropathic pain, but its mechanism is still unclear. We tested the effect of amitriptyline on 5-hydroxytryptamine 3 (5-HT3) receptor currents and studied its blocking mechanism because the clinical applications of amitriptyline overlapped with 5-HT3 receptor therapeutic potentials. Using a whole-cell voltage clamp method, we recorded the currents of the 5-HT3 receptor when 5-HT was applied alone or co-applied... -
Lamotrigine, an antiepileptic drug, inhibits 5-HT3 receptor currents in NCB-20 neuroblastoma cells
KiJungKim, SeungHyunJeun, Ki-WugSung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.2 4 169-177 (9 pages)
Lamotrigine is an antiepileptic drug widely used to treat epileptic seizures. Using whole-cell voltage clamp recordings in combination with a fast drug application approach, we investigated the effects of lamotrigine on 5-hydroxytryptamine (5-HT)3 receptors in NCB-20 neuroblastoma cells. Coapplication of lamotrigine (1~300 μM) resulted in a concentration-dependent reduction in peak amplitude of currents induced by 3 μM of 5-HT for an IC50 value of 28.2±3.6 μM with a Hill coefficient of... -
Mechanisms of Motility Change on Trinitrobenzenesulfonic Acid- Induced Colonic Inflammation in Mice
GabJinCheon, YuanCui, Dong-SooYeon, Seong-ChunKwon, Byong-GonPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.6 10 437-446 (10 pages)
Ulcerative colitis is an inflammatory bowel disease (IBD) characterized by recurrent episodes of colonic inflammation and tissue degeneration in human or animal models. The contractile force generated by the smooth muscle is significantly attenuated, resulting in altered motility leading to diarrhea or constipation in IBD. The aim of this study is to clarify the altered contractility of circular and longitudinal smooth muscle layers in proximal colon of trinitrobenzen sulfonic acid...
