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Comparisonbetween Dot-immunoblotting Assay and Clinical Sign Determination Method forQuantifying Avian Infectious Bronchitis Virus Vaccine
Seong su Yuk, Jung Hoon Kwon, Jin Yong Noh, Woo tack Hong, Gyeong Bin Gwon, Jei Hyun Jeong, Ha Na Youn, Chang Seon Song 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 188 434-435 (2 pages)
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Genetic Characterization of Salmonella Enteritidis and Salmonella Typhimurium Isolated from Samples of Slaughtered Animals during 2003-2008 in Korea
Sia Kang, Heui Jin Kim, Suk Kyung Lim, Kichan Lee, Dong Chan Moon, Hee Soo Lee, Hyang Mi Nam 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 96 188-189 (2 pages)
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Minimum Infectious Dose Determination of the K40/09Infectious bronchitis Virus vaccine candidate strain by spray administration
Jun-young Kim, Ha-na Youn, Woo-tack Hong, Hyo-sun Ju, Kyu-jik Kim, Seoung-su Yuk, Jung-hoon Kwon, Jin-yong Noh, Je-hyeon Jung, Sol Jeong, 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 157 296-296 (1 pages)
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Identification, sequence analysis, and infectivity of H9N2 avian influenza viruses isolated from geese
Rui Zhu, Xueqin Yang, Jianjun Zhang, Danwen Xu, Jiawen Fan, Huoying Shi, Shifeng Wang, Xiufan Liu 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 10 406-415 (10 pages)
The subtype H9N2 avian influenza virus greatly threatens the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three H9N2 virus strains, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the... -
Experimental infection and pathology of clade 2.2 H5N1 virus in gulls
Marina A. Gulyaeva, Kirill A. Sharshov, Anna V. Zaykovskaia, Lidia V. Shestopalova, Aleksander M. Shestopalov 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 7 179-188 (10 pages)
During 2006, H5N1 HPAI caused an epizootic in wild birds, resulting in a die-off of Laridae in the Novosibirsk region at Chany Lake. In the present study, we infected common gulls (Larus canus) with a high dose of the H5N1 HPAI virus isolated from a common gull to determine if severe disease could be induced over the 28 day experimental period. Moderate clinical signs including diarrhea, conjunctivitis, respiratory distress and neurological signs were observed in virus-inoculated birds, and 50%... -
Prolonged excretion of a low-pathogenicity H5N2 avian influenza virus strain in the Pekin duck
José Manuel Carranza-Flores, Luis Padilla-Noriega, †, Elizabeth Loza-Rubio, Gary García-Espinosa 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 16 487-490 (4 pages)
were experimentally inoculated with an H5N2 LPAIV that causes death in embryonated chicken eggs (A/chicken/Mexico/2007). Leghorn chickens were used as susceptible host controls. The degree of viral excretion was evaluated with real-time reverse transcriptase-polymerase chain reaction (RRT-PCR) using samples from oropharyngeal and cloacal swabs. We observed prolonged excretion from both species of birds lasting for at least 21 days. Prolonged excretion of LPAIV A/chicken/Mexico/2007 is atypical. -
Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine
Fang Yan*, Yujun Zhao, Yongting Hu, Jianyang Qiu, Wenxin Lei, Wenhui Ji, Xuying Li, Qian Wu, Xiumin Shi, Zhong Li 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 8 53-60 (8 pages)
The protective efficacy of DNA plasmids encoding avian infectious bronchitis virus (IBV) S1, N, or M protein was investigated in chickens. Chickens were inoculated monovalently (with plasmid pVAX1-16S1, pVAX1-16M, or pVAX1-16N alone) or multivalently (combination of the three different plasmids, pVAX1-16S1/M/N). A prime-boost immunization protocol against IBV was developed. Chickens were immunized with the multivalent DNA vaccine twice and then boosted with an inactivated vaccine once. Antibody... -
Antigenic and immunogenic investigation of the virulence motif of the Newcastle disease virus fusion protein
Kang-Seuk Choi*, Eun-Kyoung Lee, Woo-Jin Jeon, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 5 205-211 (7 pages)
Newcastle disease (ND) caused by virulent Newcastle disease virus (NDV) is a highly contagious viral disease of poultry. Virulent NDVs characteristically have a multibasic amino acid sequence (virulence motif) such as RRQKRF at the cleavage site of the precusor fusion (F0) protein. The antigenic and immunogenic characteristics of the virulence motif RRQKRF in the F0 protein of virulent NDVs were investigated. Epitope mapping analysis revealed that a RRQKRF-specific monoclonal antibody 4G2... -
Molecular identification of the vaccine strain from the inactivated oil emulsion H9N2 low pathogenic avian influenza vaccine
Jun-Gu Choi, Youn-Jeong Lee, Ji-Yeon Kim, Yeon-Hee Kim, Mi-Ra Paek, Dong-Kun Yang, Seong-Wan Son, Jae-Hong Kim 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 11 161-163 (3 pages)
In order to control the H9N2 subtype low pathogenic avian influenza (LPAI), an inactivated vaccine has been used in Korea since 2007. The Korean veterinary authority permitted the use of a single H9N2 LPAI vaccine strain to simplify the evolution of the circulating virus due to the immune pressure caused by the vaccine use. It is therefore important to determine the suitability of the vaccine strain in the final inactivated oil emulsion LPAI vaccine. In this study, we applied molecular rather... -
Pathogenicity and antigenicity of a new variant of Korean nephropathogenic infectious bronchitis virus
Kang-Seuk Choi*, Eun-Kyoung Lee, Woo-Jin Jeon, Mi-Ja Park, Jin-Won Kim, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 14 357-359 (3 pages)
Despite the existence of an active vaccination program, recently emerged strains of nephropathogenic infectious bronchitis virus (IBV) in Korea have caused significant economic losses in the poultry industry. In this study, we assessed the pathogenic and antigenic characteristics of a K-IIb type field strain of IBV that emerged in Korea since 2003, such as Kr/Q43/06. Specific pathogen free 1-week- old chickens exhibited severe respiratory symptoms (dyspnea) and nephropathogenic lesions (swollen... -
Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand
Tawatchai Pohuang, Niwat Chansiripornchai*, Achara Tawatsin, Jiroj Sasipreeyajan 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 7 219-223 (5 pages)
Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and... -
Experimental infection of chickens, ducks and quails with the highly pathogenic H5N1 avian influenza virus
Ok-Mi Jeong, Min-Chul Kim, Min-Jeong Kim, Hyun-Mi Kang, Hye-Ryoung Kim, Yong-Joo Kim, Seong-Joon Joh, Jun-Hun Kwon, Youn-Jeong L 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 8 53-60 (8 pages)
Highly pathogenic avian influenza viruses (HPAIV) of the H5N1 subtype have spread since 2003 in poultry and wild birds in Asia, Europe and Africa. In Korea, the highly pathogenic H5N1 avian influenza outbreaks took place in 2003/2004, 2006/2007 and 2008. As the 2006/2007 isolates differ phylogenetically from the 2003/2004 isolates, we assessed the clinical responses of chickens, ducks and quails to intranasal inoculation of the 2006/2007 index case virus, A/chicken/Korea/IS/06. All the chickens... -
Seroprevalence of low pathogenic avian influenza (H9N2) and associated risk factors in the Gyeonggi-do of Korea during 2005-2006
Jong-Tae Woo, Bong Kyun Park* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 6 161-168 (8 pages)
p = 0.017). The farmers recognized at least one of the clinical signs in seropositive flocks, such as decreased egg production, respiratory syndromes, and increased mortality (OR = 2.3, p = 0.082). In a matched case-control study, 20 pairs of case and control flocks matched for type of flock, hired employees, age, and flock size were compared. Frequent cleansing with disinfectants was associated with a decreased risk of seropositivity (OR = 0.2, p = 0.022). Although there was a low statistical... -
An inactivated vaccine to control the current H9N2 low pathogenic avian influenza in Korea
Jun Gu Choi, Youn Jeong Lee, *, Yong Joo Kim, Eun Kyoung Lee, Ok Mi Jeong, Haan Woo Sung, Jae Hong Kim, Jun Hun Kwon1 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 9 67-74 (8 pages)
The H9N2 subtype low pathogenic avian influenza is one of the most prevalent avian diseases worldwide, and was first documented in 1996 in Korea. This disease caused serious economic loss in Korea's poultry industry. In order to develop an oil-based inactivated vaccine, a virus that had been isolated in 2001 (A/chicken/Korea/01310/ 2001) was selected based on its pathogenic, antigenic, and genetic properties. However, in animal experiments, the efficacy of the vaccine was found to be very low... -
Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea
Ji-Hyun Jang, Haan-Woo Sung, Chang-Seon Song, Hyuk-Moo Kwon* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 13 401-408 (8 pages)
Twelve Korean infectious bronchitis viruses (IBVs) were isolated in the field from chickens suspected of being carriers of infectious bronchitis between 2001 and 2003. The S1 glycoprotein genes of these IBV isolates were amplified by reverse transcriptase-polymerase chain reaction (RT- PCR) and analyzed by restriction fragment length polymorphism (RFLP) analysis. These Korean IBV isolates were classified into three groups according to their RFLP patterns obtained using the restriction enzyme... -
Some observations on the adaptation of Eimeria tenella (local isolates) sporozoites on chicken embryos through chorioallantoic membrane
M.Abdul Hafeez, Masood Akhtar, M.Mazhar Ayaz 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 10 59-61 (3 pages)
Eimeria (E.)tenella (local isolate)sporzoites were adapted on the chorioallantoic membrane (CAM)of 10-12 days chicken embryos and completed its life cycle in 6~7 days at 39oC and 70 per cent humidity.Only 23 embryos (4.6%)were found dead from 1~4 day post inoculation of sporozoites with mild lesions on CAM with no gametocytes but few sporozoites in chorioallantoic fluid (CAF).On 5~7 day post inoculation, 432 embryos (86.4%)were found dead with severe haemorrhages on CAM and CAF contained... -
Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency
Mi-ja Park , Seong-joon Joh , Kang-seuk Choi , Aeran Kim , Min-goo Seo , Jae-young Song , Seon-jong Yun 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 4 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2016, 제 56권 제 3호 9 189-192 (4 pages)
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Surveillance and molecular epidemiology of avian influenza viruses from birds in zoos, backyard flocks and live bird markets in Korea
Jin Wook Jang , Il Hwan Kim , Hyuk Joon Kwon , Seung Min Hong , Jae Hong Kim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 14 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2012, 제 52권 제 4호 4 239-252 (14 pages)
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Genetic and biological characteristics of recent Korean isolates of avian influenza virus subtype H9N2
Madhav Prasad Acharya , Hyuk Joon Kwon , Il Hwan Kim , Youn Jeong Lee , Jae Hong Kim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2012, 제 52권 제 4호 1 223-230 (8 pages)
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Isolation and characterization of fowl adenovirus serotype 4 from chickens with hydropericardium syndrome in Korea
Hong Su Park , Il Soo Lim , Sang Kyu Kim , Toh Kyung Kim , Sang Geon Yeo 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2011, 제 51권 제 3호 5 209-216 (8 pages)
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원저 : 한국호흡기형 닭전염성기관지염 생독백신주의 작성
최강석, 전우진, 이은경, 계수정, 박미자, 권준헌 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2011, 제 51권 제 3호 3 193-201 (9 pages)
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국내에서 분리한 Ornithobacterium rhinotracheale 균의 종란과 육계에서의 병원성
권용국, 전우진, 강민수, 오재영, 안병기, 송은아, 권준헌, 이청산, 김재홍, Kwon. Yong-Kuk, Jeon. Woo-Jin, Kang. Min-Soo, Oh. Jae-Young, An. Byung-Ki, Song. Eun-A, Kwon. Jun- 한국가금학회 한국가금학회지 7 Pages
한국가금학회 한국가금학회지 2010, Vol.37 No.2 159-165 (7 pages)
본 시험은 국내에서 분리한 Ornithobacterium rhinotracheale(OR) 병원성 세균이 부화중인 종란과 일반 육계에서 얼마만큼 병원성을 보이는지 알아보기 위하여 수행하였다. 첫 번째 9일령 부화란의 yolk sac에 국내에서 분리한 3종의 OR strain을 접종하여 12일동안 관찰한 결과, 66% 이상의 폐사율이 관찰되어 높은 병원성이 인정되었다. 두 번째로 3주령 일반 육계를 대상으로 5가지 다른 공격접종법[Intratracheal, Intravenous, Intramuscular, Aerosol, Newcastle Disease Virus(NDV)와 혼합 Aerosol]으로 접종한 다음 병원성을...
