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Differential patterns of nestin and glial fibrillary acidic protein expression in mouse hippocampus during postnatal development
Joong-Sun Kim, Juhwan Kim, Yujin Kim, Miyoung Yang, Hyosun Jang, Sungwoon Kang, Jong-Choon Kim, Sung-Ho Kim, Taekyun Shin*, Chan 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 1 1-6 (6 pages)
Intermediate filaments, including nestin and glial fibrillary acidic protein (GFAP), are important for the brain to accommodate neural activities and changes during development. The present study examined the temporal changes of nestin and GFAP protein levels in the postnatal development of the mouse hippocampus. Mouse hippocampi were sampled on postnatal day (PND) 1, 3, 6, 18, and 48. Western blot analysis showed that nestin expression was high at PND 1 and markedly decreased until PND 18.... -
Protein Patterns of Rice(Oryza sativa L.) Cultivars as Affected by Herbicide Thiobencarb
김학윤, 김길웅, 신동현, 김건우, Kim. H.Y., Kim. K.U., Shin. D.H., Kim. K.W. 한국잡초학회 한국잡초학회지 8 Pages
한국잡초학회 한국잡초학회지 1996, Vol.16 No.4 354-361 (8 pages)
Thiobencarb 처리에 의한 수도품종의 내성 정도를 조사하고 내성 메카니즘을 연구하기 위하여 내성 및 감수성 품종간의 단백질 함량, SDS-PAGE에 의한 단백질 유형의 변화를 조사하였다. 본 시험에 공시한 5개 품종 중 IR 10198-66-2와 IR 9660-50-3-1은 온실 및 실내실험에서 공히 thiobencarb에 대하여 내성을 보였으며 IR 22, IR 31802-48-2-2, IR 20656-R-R-R-6-1은 제초제 농도가 증가함에 따라 생장이 크게 억제되어 감수성을 나타내었다. Thiobencarb 처리에 의한 단백질의 함량은 내성 및 감수성 품종간에 뚜렷한 차이를 보였는데... -
The Protein and Isozyme Patterns During in vitro Plant Regeneration of Yooja (Citrus junos Sieb.) and Trifoliate Orange (Poncirus trifoliata Rafin.)
Park. Min-Hee, Jang. Hyun-Kyu, Cha. Young-Ju, Kim. Ho-Bun, Lee. Sook-Young 한국자원식물학회 Plant resources 16 Pages
한국자원식물학회 Plant resources 2002, Vol.5 No.1 29-44 (16 pages)
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Classification of Meat-Based Listeria monocytogenes Using Whole-Cell Protein Patterns and Serotyping Analysis
Park. Si-Hong, Jung. Sang-Hoon, Kim. Hyun-Joong, Chung. Yun-Hee, Kim. Hae-Yeong 한국식품과학회 Food science and biotechnology 4 Pages
한국식품과학회 Food science and biotechnology 2006, Vol.15 No.2 324-327 (4 pages)
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Protein Patterns on a Vaginal Mucus during Spontaneous and Estrus Synchronization using CIDR in Korean Native Cattle (Hanwoo)
Chung. Hak-Jae, Kim. Nam-Kuk, Lee. Hwi-Cheul, Yoon. Hyun-Il, Lee. Suk-Dong, Ko. Jin-Sung, Kwon. Hyeok-Jin, Oh. Hae-Ryong, Choy. Yun-Ho, C 한국수정란이식학회 韓國受精卵移植學會誌 5 Pages
한국수정란이식학회 韓國受精卵移植學會誌 2008, Vol.23 No.4 251-255 (5 pages)
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Altered Protein Expression Patterns of Mycobacterium tuberculosis Induced by ATB107
Shen. Hongbo, Yang. Enzhuo, Wang. Feifei, Jin. Ruiliang, Xu. Shengfeng, Huang. Qiang, Wang. Honghai 한국미생물학회 The journal of microbiology 10 Pages
한국미생물학회 The journal of microbiology 2010, Vol.48 No.3 337-346 (10 pages)
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Identification and Distribution of Bacillus Species in Doenjang by Whole-Cell Protein Patterns and 16S rRNA Gene Sequence Analysis
Kim. Tae-Woon, Kim. Young-Hoon, Kim. Sung-Eon, Lee. Jun-Hwa, Park. Cheon-Seok, Kim. Hae-Yeong 한국미생물생명공학회 Journal of microbiology and biotechnology 5 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2010, Vol.20 No.8 1210-1214 (5 pages)
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Proteomic Analysis of Protein Expression Patterns Associated with Astaxanthin Accumulation by Green Alga Haematococcus pluvialis (Chlorophyceae) Under High Light Stress
Kim. Jeong-Dong, Lee. Woo-Sung, Kim. Beob-Min, Lee. Choul-Gyun 한국미생물생명공학회 Journal of microbiology and biotechnology 7 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2006, Vol.16 No.8 1222-1228 (7 pages)
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Systematic Studies of 12S Seed Storage Protein Accumulation and Degradation Patterns during Arabidopsis Seed Maturation and Early Seedling Germination Stages
Li. Qing, Wang. Bai-Chen, Xu. Yu, Zhu. Yu-Xian 생화학분자생물학회 Journal of biochemistry and molecular biology 9 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 2007, Vol.40 No.3 373-381 (9 pages)
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GPS Collars of Cloven-Hoofed Livestock Movement Patterns and Prevention of Foot and Mouth Disease in Eastern Mongolia
Suvd Erdene Tseren Ochir, Oun Kyong Moon, Woo seog Jeong, Hac hung Yoon, Jida Choi, Yong Myong Kang, Erdene Ochir Tseren Ochir, Ganzori 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 174 426-427 (2 pages)
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Novel Polymorphism in Korean Water Deer Prion Protein Gene
In Soon Roh, Hyun Joo Sohn, Hyun Jeong Kim, Tae Young Suh, Hyo Jin Kim, In Soo Cho, Byoung han Kim 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 173 426-426 (1 pages)
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Patterns of Antimicrobial Resistance and Transfer of Resistance Genes in Tetracycline-resistant Escherichia coli Isolated from Beef Cattle in South Korea
Seung Won Shin, Kuastros Mekonnen Balaynehe, Myung hwan Jung, Han Sang Yoo 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 44 160-161 (2 pages)
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Activation of autophagy flux by Ophiopogonin B downregulates cellular FLICE–like inhibitory protein and enhances TRAIL-induced apoptosis
Mohammad Rasheduzzaman, Jeong Min Hong, Uddin Nazim, Hong hua Yin, You Jin Lee, Jae Won Seol, Sang Youel Park 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 238 340-341 (2 pages)
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Analysis of protein expression in Brucella abortus mutants with different growth rates by two-dimensional gel electrophoresis and LC-MS/MS peptide analysis
Woo Bin Park, Young Bin Im, Soojin Shim, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 16 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 8 216-231 (16 pages)
mutant and wild-type B. abortus were purified and subjected to two-dimensional gel electrophoresis. Thirty protein spots of each mutant with expression increases or decreases were selected; those with a change of more than 2-fold were compared with the wild-type. Selected spots underwent liquid chromatography tandem mass spectrometry for peptide analysis. DnaK and ClpB, involved in protein aggregation, increased. SecA and GAPDH, associated with energy metabolism, decreased in some mutants with a... -
Polymorphism in the serotonin transporter protein gene in Maltese dogs with degenerative mitral valve disease
Chang-Min Lee, Jae-Ik Han, Min-Hee Kang, Seung-Gon Kim, Hee-Myung Park 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 16 129-135 (7 pages)
five nonsynonymous genetic variations, were detected in five exons. Protein structure and function of the five nonsynonymous genetic variations were predicted. Three of the five polymorphisms were predicted to be probable causes of damage to protein function and confirmed by protein structure model verification. This study identified six polymorphisms of the SERT gene in Maltese dogs with DMVD, suggesting an association between the SERT gene and canine DMVD. This is the first study of SERT... -
Coactosin-like protein 1 inhibits neuronal migration during mouse corticogenesis
Guohong Li, Yupeng Yin, Jiong Chen, Yanle Fan, Juhong Ma, Yingxue Huang, Chen Chen, Pengxiu Dai, Shulin Chen, Shanting Zhao 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 4 21-26 (6 pages)
Coactosin-like protein 1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from a soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains undescribed. In this study, we revealed that Cotl1 overexpression impaired migrationof both early- and late-born neurons during mouse corticogenesis.... -
Downregulation of cellular prion protein inhibited the proliferation and invasion and induced apoptosis of Marek’s disease virus-transformed avian T cells
Xuerui Wan, Runxia Yang, Guilin Liu, Manling Zhu, Tianliang Zhang, Lei Liu, Run Wu* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 6 171-178 (8 pages)
Cellular prion protein (PrP) is ubiquitously expressed in the cytomembrane of a considerable number of eukaryotic cells. Although several studies have investigated the functions of PrP in cell proliferation, cell apoptosis, and tumorigenesis of mammals, the correlated functions of chicken PrP (chPrP) remain unknown. In this study, stable chPrP-downregulated Marek’s disease (MD) virus-transformed avian T cells (MSB1-SiRNA-3) were established by introducing short interfering RNA (SiRNA) targeting... -
Molecular characterization of duck enteritis virus CHv strain UL49.5 protein and its colocalization with glycoprotein M
Meng Lin, Renyong Jia*, Mingshu Wang, Xinghong Gao, Dekang Zhu, Shun Chen, Mafeng Liu, Zhongqiong Yin, Yin Wang, Xiaoyue Chen, A 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 7 389-398 (10 pages)
constructed and used to transfect duck embryo fibroblasts. Next, the recombinant plasmid pDsRed1-N1/ glycoprotein M (gM) was produced and used for co-transfection with the pEGFP-C1/pUL49.5 plasmid to determine whether DEV pUL49.5 and gM (a conserved protein in herpesviruses) colocalize. DEV pUL49.5 was thought to be an envelope glycoprotein with a signal peptide and two transmembrane domains. This protein was also predicted to localize in the cytoplasm and endoplasmic reticulum with a probabilit... -
Tissue-specific expression of the NOD-like receptor protein 3 in BALB/c mice
Zhiqiang Huang, Meng Yu, Shuang Tong, Kun Jia, Rongchang Liu, Heng Wang, Shoujun Li*, Zhangyong Ning* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 1 173-177 (5 pages)
of pathogen-associated molecular patterns, such as NOD-like receptors. The NOD-like receptor protein 3 (NLRP3) inflammasome is involved in induction of the pro- inflammatory cytokine, IL-1β, and subsequent inflammatory responses. NLRP3 inflammasome plays important roles in the inflammatory and innate immune responses associated with autoimmune/inflammatory syndrome. However, analysis of the tissue distribution and expression profiles in BALB/c mice is still incomplete. In this study, we... -
A simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos
Sang Kyu Park, Sangho Roh*, Jong-Im Park* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 8 73-80 (8 pages)
Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression levels of developmentally important genes that may affect embryonic development and pregnancy. In the present study, porcine oocytes were treated with demecolcine that facilitated enucleation with protruding genetic material. Enucleation and donor... -
Development of monoclonal antibodies against the abnormal prion protein isoform (PrP) associated with chronic wasting disease (CWD)
Hyun-Jeong Jeong, Nak-Hyung Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Kun-Ho Seo, Dong-Woon Kim, Yong-Sun Kim, In-So 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 14 429-432 (4 pages)
Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to... -
Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle
Sangho Choi, Hee-Jong Woo*, Jeongmin Lee* 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 3 127-137 (11 pages)
we investigated bovine prion protein gene (PRNP) variations in Hanwoo cattle [Bos (B.) taurus coreanae], a native breed in Korea. We identified mutations and polymorphisms in the coding region of PRNP, determined their frequency, and evaluated their significance. We identified four synonymous polymorphisms and two non-synonymous mutations in PRNP, but found no novel polymorphisms. The sequence and number of octapeptide repeats were completely conserved, and the haplotype frequency of the coding... -
Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs
Gulay Ciftci*, Gul Fatma Yarim 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 4 325-331 (7 pages)
we measured the insulin-like growth factor (IGF)-I levels and evaluated the serum protein profiles of diabetic, insulin-treated, and healthy cats and dogs. The total IGF-I concentrations were 33.74 ± 3.4 ng/mL for normal, 25.8 ± 4.5 ng/mL for diabetic, and 180.4 ± 31.4 ng/mL for insulin-treated cats. IGF-I concentrations were 46.4 ± 6.6 ng/mL for normal, 25.1 ± 4.1 ng/mL for diabetic, and 303.0 ± 61.3 ng/mL for insulin-treated dogs. Total serum protein profiles were analyzed by SDS-PAGE.... -
Sequence and phylogenetic analysis of the gp200 protein of Ehrlichia canis from dogs in Taiwan
Chia-Chia Huang, Yu-Chen Hsieh, Chau-Loong Tsang, Yang-Tsung Chung* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 9 333-340 (8 pages)
the gp200 gene, which encodes the largest major immunoreactive protein in E. canis, from 17 Taiwanese samples was conducted. The resultant 16S rDNA sequences were found to be identical to each other and have very high homology (99.4∼100%) with previously reported E. canis sequences. Additionally, phylogenetic analysis of gp200 demonstrated that the E. canis Taiwanese genotype was genetically distinct from other reported isolates obtained from the United States, Brazil, and Israel, and that... -
Antigenic diversity of Theileria major piroplasm surface protein gene in Jeju black cattle
Myung-Soon Ko, Kyoung-Kap Lee, Kyu-Kye Hwang, Byung-Sun Kim, Gui-Cheol Choi, Young-Min Yun* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 5 155-160 (6 pages)
In May 2003, we examined the antigenic diversity of the major piroplasm surface protein (MPSP) gene in 35 healthy Jeju black cattle that were born and raised at the National Institute of Subtropical Agriculture. On microscopic examination of Giemsa-stained blood smears, 9 of 35 cattle had intra-erythrocytic piroplasms. Hematological data were within normal range for all 35 cattle. Amplification of DNA from all blood samples using universal MPSP gene primers showed mixed infections with C, I, and... -
Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus)
Hyun-Jeong Jeong, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Bo-Sook Kim, Jung-Rae Rho, Mi-Hyun Yoo, Byung-Hoon Jeong, Yon 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 16 299-301 (3 pages)
of the prion protein gene (PRNP) have been detected in several cervid species. In order to confirm the genetic variations, this study examined the DNA sequences of the PRNP obtained from 33 captive sika deer (Cervus nippon laiouanus) in Korea. A total of three single-nucleotide polymorphisms (SNPs) at codons 100, 136 and 226 in the PRNP of the sika deer were identified. The polymorphic site located at codon 100 has not been reported. The SNPs detected at codons 100 and 226 induced amino acid... -
Increased phosphorylation of c-Jun NH (2)-terminal protein kinase in the sciatic nerves of Lewis rats with experimental autoimmune neuritis
Meejung Ahn, Taekyun Shin 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 2 13-17 (5 pages)
(2)-terminal protein kinase (JNK), one of the mitogen-activated protein kinases, was analyzed in the sciatic nerves of Lewis rats with experimental autoimmune neuritis (EAN).Western blot analysis showed that the expression levels of both phosphorylated JNK1 (p-JNK1, approximately 46 kDa)and phosphorylated JNK2 (p-JNK2, approximately 54 kDa)in the sciatic nerves of rats with EAN increased significantly (p <0.05)at day 14 post-immunization (PI)and remained at this level at days 24 and 30 PI, with... -
Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2
Sook-Yeon Lee, Jin-Young Chung, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Hwa-Young Youn, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 8 141-145 (5 pages)
The research of p53 is being conducted to find the mechanisms of tumorigenesis and to treat various cancers. Homeodomain-interacting protein kinase2 (HIPK2) is an important factor to regulate p53 and to increase the stability of p53. Activation of HIPK2 leads to the selective phosphorylation of p53, resulting in growth arrest and the enhancement of apoptosis. In this study, the canine HIPK2 cDNA fragments were obtained, and their overlapping regions were aligned to give a total sequence of 3489... -
Cap-independent protein translation is initially responsible for 4-(N-methylnitrosamino)-1-(3-pyridyl)-butanone (NNK)-induced apoptosis in normal human bronchial pithelial cells
Seo-Hyun Moon, Hyun-Woo Kim, Jun-Sung Kim, Jin-Hong Park, Hwa Kim, Gook-Jong Eu, Hyun-Sun Cho, Ga-Mi Kang, Kee-Ho Lee, Myung-Hai 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 13 369-378 (10 pages)
Evidences show that eukaryotic mRNAs can perform protein translation through internal ribosome entry sites (IRES). 5'-Untranslated region of the mRNA encoding apoptotic protease-activating factor 1 (Apaf-1) contains IRES, and, thus, can be translated in a cap-independent manner. Effects of changes in protein translation pattern through rapamycin pretreatment on 4-(methylnitrosamino)-1-(3-pyridyl)-butanone(NNK, tobacco-specific lung carcinogen)-induced apoptosis in human bronchial epithelial... -
Characterization of Antigenic Sites on the Rinderpest Virus N Protein Using Monoclonal Antibodies
Kang-seuk Choi, Jin-ju Nah, Young-joon Ko, Cheong-up Choi, Jae-hong Kim, Shien-young Kang, Yi-seok Joo 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 9 57-65 (9 pages)
SDS exhibited higher ELISA titers in all Mabs recognizing 6 sites. Four sites (A, B, E and F) on 2% SDS-treated N protein lost completely reactivity with Mabs while the remaining sites (C and D) on the protein retained their antigenicity to some degree. It indicates that two sites (C and D) were sequential. Six representative Mabs bound to each site exhibited competition with rinderpest antibodies in a blocking ELISA, indicating that the sites were actively involved in antigenicity in cattle. -
Immunohistochemical Localization of Nerve Growth Factor,Glial Fibrillary Acidic Protein and Ciliary Neurotrophic Factor in Mesencephalon, Rhombencephalon, and Spinal Cord of Developing Mongolian Gerbil
Il-Kwon Park, Kyoug-Youl Lee, Chi-Won Song, Hyo-Jung Kwon, Mi-Sun Park, Mi-Young Lee, Keun-Jwa Lee, Young-Gil Jeong, Chul-Ho Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 14 239-245 (7 pages)
The distribution of the nerve growth factor (NGF), the glial fibrillary acidic protein (GFAP) and the ciliary neurotrohic factor (CNTF) was performed in coronal sections of the mesencephalon, rhombencephalon and spinal cord in the developing Mongolian gerbils. Generally, NGF specifically recognizes neurons with the NGF receptor, whereas GFAP does the glia, and CNTF does the motor neurons. The receptor expression was examined separately in gerbils between embryonic days 15 (E15) and postnatal...
