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본문검색 결과만 보기
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Mass Spectrometry Based Quantitative Proteomic Profiling of Hepatocellular Carcinoma Cell Line
Suchismita Raha, Ho Jeong Lee, Venu Venkatarame Gowda Saralamma, Silvia Yumnam, Jeong Doo Heo, Sang Joon Lee, Eun Hee Kim, Gon Sup Kim 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 춘계학술심포지움 92 134-135 (2 pages)
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Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 9 59-70 (12 pages)
mutant B. henselae (Δomp43) was generated to investigate OMP43 function. Interestingly, Δomp43 exhibited weaker proliferative ability than that of wild-type (WT) B. henselae. To study the differences in proteomic expression between WT and Δomp43, two-dimensional gel electrophoresis-based proteomic analysis was performed. Based on Clusters of Orthologus Groups functional assignments, 12 proteins were associated with metabolism, 7 proteins associated with information storage and processing, and... -
Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis
Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)
severe E. coli mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel electrophoresis and label-free proteomic approaches. A total of 95 differentially expressed proteins were identified. Of these, 56 proteins were categorized according to molecular function, cellular component, and biological processes. The most frequent biological processes influenced by the proteins were response to stress, transport, and establishment of localization. Furthermore, a... -
Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
Xiaoyu Deng, Yanlong Cong, Renfu Yin, Guilian Yang, Chan Ding, Shengqing Yu, Xiufan Liu, Chunfeng Wang, †, Zhuang Ding, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 7 511-517 (7 pages)
in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two- dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78 proteins increased more than two-fold. Thirty-five proteins exhibited consistent changes in expression and 13 were identified as unique proteins by matrix assisted laser desorption ionization-time of flight mass spectrometer/mass... -
Time-dependent proteomic and genomic alterations in Toll-like receptor-4-activated human chondrocytes: increased expression of lamin A/C and annexins
SeungHeeHa, HyoungKyuKim, NguyenThiTuyetAnh, NariKim, KyungSooKo, ByoungDooRhee, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 16 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.5 8 531-546 (16 pages)
we determined the proteomic and genomic alterations in human chondrocytes during lipopolysaccharide (LPS)-induced inflammation to elucidate the underlying mechanisms and consequences of TLR-4 activation. Human chondrocytes were cultured with LPS for 12, 24,and 36 h to induce TLR-4 activation. The TLR-4-induced inflammatory response was confirmed by real-time PCR analysis of increased interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) expression levels. In... -
Proteomic Analysis of Colonic Mucosal Tissue from Tuberculous and Ulcerative Colitis Patients
Seong-ChunKwon, KyungJongWon, SeoungHyoJung, KangPaLee, Dong-YoubLee, Eun-SeokPark, BokyungKim, GabJinCheon, KoonHeeHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.3 7 193-198 (6 pages)
Changes in the expression profiles of specific proteins leads to serious human diseases, including colitis. The proteomic changes related to colitis and the differential expression between tuberculous (TC) and ulcerative colitis (UC) in colon tissue from colitis patients has not been defined. We therefore performed a proteomic analysis of human TC and UC mucosal tissue. Total protein was obtained from the colon mucosal tissue of normal, TC, and UC patients, and resolved by 2-dimensional... -
Proteomic Analysis of Differentially Expressed Proteins in Bovine Endometrium with Endometritis
ChangyongChoe, Jeong-WonPark, Eun-SukKim, Sung-GyuLee, Sun-YoungPark, Jeong-SoonLee, Myung-JeCho, KeeRyeonKang, JaeheeHan, DawonKang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.4 2 205-212 (8 pages)
and immunoblot analysis. The mRNA levels of desmin, transferrin, Ձ-actin-2, HSP27, KCTD11, and MHC-Ih were up-regulated by over 1.5-fold, and showed a pattern similar to their proteomic profiles. Desmin and Ձ-actin-2 protein showed positive correlations between proteomic analysis and immunoblot analysis. These results suggest that desmin and Ձ-actin-2 may play important roles in endometritis-related function, and could be useful markers for the diagnosis of bovine endometritis. -
Comparative Proteomic Analysis for a Putative Pyridoxal Phosphate-Dependent Aminotransferase Required for Virulence in Acidovorax citrulli
Jongchan Lee, Lynn Heo, Sang-Wook Han 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 16 673-680 (8 pages)
to its substrates and uses pyridoxal phosphate as a coenzyme. It was observed that a ppdaAc knockout mutant had a significantly reduced virulence in watermelon when introduced via germinated-seed inoculation as well as leaf infiltration. Comparative proteomic analysis predicted the cellular mechanisms related to PpdaAc. Apart from causing virulence, the PpdaAc may have significant roles in energy production, cell membrane, motility, chemo- taxis, post-translational modifications, and iron-relate... -
Proteomic and Phenotypic Analyses of a Putative Glycerol-3-Phosphate Dehydrogenase Required for Virulence in Acidovorax citrulli
Minyoung Kim, Jongchan Lee, Lynn Heo, Sang Jun Lee, Sang-Wook Han 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 1호 3 36-46 (11 pages)
in Ac (GlpdAc) using comparative proteomic analysis and phenotypic observation. A glpdAc knockout mutant, AcΔglpdAc(EV), lost virulence against watermelon in two pathogenicity tests. The putative 3D structure and amino acid sequence of GlpdAc showed high similarity with glycerol-3-phosphate dehydrogenases from other bacteria. Comparative proteomic analysis revealed that many proteins related to various metabolic pathways, including carbohydrate metabolism, were affected by GlpdAc. Although... -
Proteomic Reference Map and Comparative Analysis between Streptomyces griseus S4-7 and wbiE2 Transcription Factor-Mutant Strain
Jisu Kim, Young Sang Kwon, Dong-Won Bae, Youn-Sig Kwak 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 2호 8 185-191 (7 pages)
Streptomyces griseus S4-7, a well-characterized key- stone taxon among strawberry microbial communi- ties, shows exceptional disease-preventing ability. The whole-genome sequence, functional genes, and bioactive secondary metabolites of the strain have been described in previous studies. However, proteomics studies of not only the S4-7 strain, but also the Streptomyces genus as a whole, remain limited to date. Therefore, in the pres- ent study, we created a proteomics reference map for S.... -
Proteomic Changes in the Sound Vibration-Treated Arabidopsis thaliana Facilitates Defense Response during Botrytis cinerea Infection
Ritesh Ghosh, Bosung Choi, Young Sang Kwon, Tufail Bashir, Dong-Won Bae, Hanhong Bae 한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 7 609-622 (14 pages)
Sound vibration (SV) treatment can trigger various molecular and physiological changes in plants. Previously, we showed that pre-exposure of Arabidopsis plants to SV boosts its defense response against Botrytis cinerea fungus. The present study was aimed to investigate the changes in the proteome states in the SV-treated Arabidopsis during disease progression. Proteomics analysis identified several upregulated proteins in the SVinfected plants (i.e., SV-treated plants carrying Botrytis... -
Identification of Proteomic Components Associated with Resistance to Fusarium Head Blight in Rye
Dawid Perlikowski, Halina Wi?, niewska;Tomasz G?ral, Piotr Ochodzki, Maciej Majka, Izabela Pawłowicz, Jolanta Belter, Arkadiusz Kosmala 한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 4호 3 313-327 (15 pages)
of resistance to FHB were analyzed using two-dimensional gel electrophoresis and mass spectrometry to identify resistance markers. The proteome profiling was accompanied by measurements of a- and b-amylase activities and mycotoxin content. The proteomic studies indicated a total of 18 spots with clear differences in protein abundance between the more resistant and more susceptible rye lines after infection. Eight proteins were involved in carbohydrate metabolism of which six proteins showed a... -
Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 6호 9 602-607 (6 pages)
Xanthomonas campestris pv. vesicatoria) using phenotypic and proteomic analyses. Growth of XcvΔscpBXv (ScpBXv knockout mutant) was reduced under both slow and fast growth conditions in rich medium, but comparable to this of the wild-type in plant-mimic conditions. Interestingly, the mutant was significantly less virulent than the wild-type in tomato, indicating that ScpBXv is involved in virulence. To investigate ScpBXv-associated mechanisms, comparative proteomic analyses were carried... -
Deciphering the Role of Tyrosine Sulfation in Xanthomonas oryzae pv. oryzae Using Shotgun Proteomic Analysis
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 11 266-272 (7 pages)
and a raxST knockout mutant using shotgun proteomic analysis. Forty nine proteins displayed a more than 1.5-fold difference in their expression between the wildtype and the mutant strains. Clusters of orthologous groups analysis revealed that proteins involved in cell motility were most abundant, and phenotypic observation also showed that the twitching motility of the mutant was dramatically changed. These results indicate that tyrosine sulfation by RaxST is essential for Xoo movement, and they... -
Proteomic Analysis of a Global Regulator GacS Sensor Kinase in the Rhizobacterium, Pseudomonas chlororaphis O6
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 2호 15 220-227 (8 pages)
The GacS/GacA system in the root colonizer Pseudomonas chlororaphis O6 is a key regulator of many traits relevant to the biocontrol function of this bacterium. Proteomic analysis revealed 12 proteins were down-regulated in a gacS mutant of P. chlororaphis O6. These GacS-regulated proteins functioned in combating oxidative stress, cell signaling, biosynthesis of secondary metabolism, and secretion. The extent of regulation was shown by real-time RT-PCR to vary between the genes. Mutants of P.... -
Proteomic Analysis of Differential Protein Expression in Fibroblast-like Synoviocytes of Pig
O, Baatartsogt., So, Hyun-Kyung, Cho, In-Hee, Lee, Jeong-Soo, Lim, Hee-Kyung, Lee, Jong-Ha, Kim, Eun-Kuk, Choi, Kung-Duk 한국축산식품학회 학술대회논문집 6 Pages
The innermost structures of synovium consist of one to three layers of cells generally identified as synovial lining cells(SLC). The present studies were initiated to determine the protein expression patterns of fibroblast-like synovial(FLS) cells derived from the synovia of rheumatoid arthritis. Post-traumatic arthritis(PTA) is one of the most common causes of secondary osteoarthritis, and usually affects younger people. The proteins were separated by two-dimensional polyacrylamide gel... -
Proteomic Analysis of Serum from Patients with Major Depressive Disorder to Compare Their Depressive and Remission Statuses
Jiyeong Lee, EunJeong Joo, HeeJoung Lim, JongMoon Park, KyuYoung Lee, Arum Park, AeEun Seok, HooKeun Lee, HeeGyoo Kang 대한신경정신의학회 Psychiatry Investigation 11 Pages
대한신경정신의학회 Psychiatry Investigation 2015, 제 12권 제 2호 14 249-259 (11 pages)
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Differential Proteomic Analysis of Secreted Proteins from Cutinase-producingBacillus sp. SB-007
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 2호 13 191-201 (11 pages)
parts of South Korea through screening on a minimal medium containing 0.2% purified cutin for its ability to induce the cutinase production. However, no cutinase was produced when it was grown in a minimal medium containing 0.2% glucose. A proteomic approach was applied to separate and characterize these differentially secreted proteins. The expression level of 83 extracellular proteins of the cutinase-producing Bacillus sp. strain SB-007 incubated in a cutinase-induced medium increased... -
Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in Saccharomyces cerevisiae
Nguyen. Ngoc-Tu, Kim. Yang-Hoon, Bang. Seung Hyuck, Hong. Ji Hye, Kwon. Soon Dong, Min. Jiho 환경독성보건학회 Environmental health and toxicology : EHT 1 Pages
환경독성보건학회 Environmental health and toxicology : EHT 2014, Vol.29 No.6 19-19 (1 pages)
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Influence of CO2 concentration on carbon concentrating mechanisms in cyanobacteria and green algae: a proteomic approach
Ramanan. Rishiram, Vinayagamoorthy. Nadimuthu, Sivanesan. Saravana Devi, Kannan. Krishnamurthi, Chakrabarti. Tapan 한국조류학회(藻類) Algae 7 Pages
한국조류학회(藻類) Algae 2012, Vol.27 No.4 295-301 (7 pages)
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An Effective Data Analysis System for Improving Throughput of Shotgun Proteomic Data based on Machine Learning
나승진, 백은옥, Na. Seung-Jin, Paek. Eun-Ok 한국정보과학회 정보과학회논문지. Journal of KIISE. 소프트웨어 및 응용 11 Pages
한국정보과학회 정보과학회논문지. Journal of KIISE. 소프트웨어 및 응용 2007, Vol.34 No.10 889-899 (11 pages)
최근 프로테오믹스 분야에서 단백질의 추출, 분리기술의 발전과 고성능 질량분석 장비로 인하여 대량으로, 또 빠르게 샘플을 분석하는 것이 가능해짐에 따라서, 한번의 실험으로부터 얻어지는 실험데이타의 양이 대폭 늘어나게 되었다. 따라서 대량의 데이타를 어떻게 처리하여 필요한 정보만을 얻어내는가가 큰 이슈가 되고 있다. 하지만 기존의 데이타 해석과정은 불필요하게 계산자원을 낭비하는 요소를 상당 부분을 포함하고 있고, 이로 인해 데이타 해석 시간이 증가함은 물론, 종종 옳지 않은 해석 결과를 생성함으로써 결과에 대한... -
Proteomic Assessment of Dung Beetle, Copris tripartitus Immune Response
Suh. Hwa-Jin, Bang. Hea-Son, Kim. Seong-Ryul, Yun. Eun-Young, Park. Kwan-Ho, Kang. Bo-Ram, Kim. Ik-Soo, Jeon. Jae-Pil, Hwang. Jae-Sam 한국잠사학회 International journal of industrial entomology 5 Pages
한국잠사학회 International journal of industrial entomology 2008, Vol.17 No.2 217-221 (5 pages)
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Proteomic Analysis of Stress Responses Protein in Wheat (Triticum aestivum L)
Kamal. Abu Hena Mostafa, Kim. Ki-Hyeon, Shin. Kwang-Hyun, Seo. Hyeong-Seok, Shin. Dong-Hoon, Hong. Lan-Seon, Park. Cheol-Soo, Jong. You 한국작물학회 Korean journal of crop science 1 Pages
한국작물학회 Korean journal of crop science 2009, Vol.54 No.1 30-30 (1 pages)
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Proteomic Approach of the Protein Profiles during Seed Maturation in Common Buckwheat (Fagopyrum esculentum Moench.)
Park. Min-Hwa, Shin. Dong-Hoon, Han. Myoung-Hae, Yun. Young-Ho, Bae. Jeong-Sook, Lee. Yun-Sang, Chung. Keun-Yook, Lee. Moon-Soon, Woo. S 한국자원식물학회 韓國資源植物學會誌 9 Pages
한국자원식물학회 韓國資源植物學會誌 2009, Vol.22 No.3 227-235 (9 pages)
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Proteomic Analysis of Seed Storage Proteins in Low Allergenic Soybean Accession
Koo. Sung-Cheol, Bae. Dong-Won, Seo. Jun-Su, Park. Kyoung-Mi, Choi. Man-Soo, Kim. Seok-Hyeon, Shim. Sang-In, Kim. Kyeong-Moon, Chung. Jo 한국응용생명화학회 Journal of the Korean Society for Applied Biological Chemistry 8 Pages
한국응용생명화학회 Journal of the Korean Society for Applied Biological Chemistry 2011, Vol.54 No.3 332-339 (8 pages)
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Proteomic Analysis of Hepatotoxicity Induced by Titanium Nanoparticles in Mouse Liver
Jeon. Yu-Mi, Park. Seul-Ki, Lee. Mi-Young 한국응용생명화학회 Journal of the Korean Society for Applied Biological Chemistry 8 Pages
한국응용생명화학회 Journal of the Korean Society for Applied Biological Chemistry 2011, Vol.54 No.6 852-859 (8 pages)
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A Proteomic Analysis of Leaf Responses to Enhanced Ultraviolet-B Radiation in Two Rice (Oryza sativa L.) Cultivars Differing in UV Sensitivity
Wu. Xing-Chun, Fang. Chang-Xun, Chen. Jin-Yang, Wang. Qing-Shui, Chen. Ting, Lin. Wen-Xiong, Huang. Zhong-Liang 한국식물학회 Journal of plant biology 11 Pages
한국식물학회 Journal of plant biology 2011, Vol.54 No.4 251-261 (11 pages)
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The Effect of DTT in Protein Preparations for Proteomic Analysis: Removal of a Highly Abundant Plant Enzyme, Ribulose Bisphosphate Carboxylase/Oxygenase
Cho. Jin-Hwan, Hwang. Hee-Youn, Cho. Man-Ho, Kwon. Yong-Kook, Jeon. Jong-Seong, Bhoo. Seong-Hee, Hahn. Tae-Ryong 한국식물학회 Journal of plant biology 5 Pages
한국식물학회 Journal of plant biology 2008, Vol.51 No.4 297-301 (5 pages)
