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Generation of a Transformant Showing Higher Manganese Peroxidase (Mnp) Activity by Overexpression of Mnp Gene in Trametes versicolor
Yeo. Su-Min, Park. Nam-Mee, Song. Hong-Gyu, Choi. Hyoung-T. 한국미생물학회 The journal of microbiology 6 Pages
한국미생물학회 The journal of microbiology 2007, Vol.45 No.3 213-218 (6 pages)
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Identification of a Cupin Protein Gene Responsible for Pathogenicity, Phage Susceptibility and LPS Synthesis of Acidovorax citrulli
Aryan Rahimi-Midani, Min-Jung Kim, Tae-Jin Choi 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 6 555-565 (11 pages)
as AC-17-G1 was isolated among 3,264 A. citrul- li Tn5 mutants through serial spot assays and plaque assays followed by pathogenicity test using seed coat- ing method. The mutant has the integrated Tn5 in the middle of a cupin protein gene. This mutant recovered its pathogenicity and phage sensitivity by complemen- tation with corresponding wild-type gene. Site-directed mutation of this gene from wild-type by CRISPR/Cas9 system resulted in the loss of pathogenicity and acquisi- tion of phage... -
A LysM Domain-Containing Protein LtLysM1 Is Important for Vegetative Growth and Pathogenesis in Woody Plant Pathogen Lasiodiplodia theobromae
Dulanjalee Lakmali Harishchandra, Wei Zhang, Xinghong Li, Kandawatte Wedaralalage Thilini Chethana, Kevin David Hyde, Siraprapa Brooks, Jiye Yan, Junbo Peng 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 4호 3 323-334 (12 pages)
are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence analyses revealed that... -
MoRBP9 Encoding a Ran-Binding Protein Microtubule-Organizing Center Is Required for Asexual Reproduction and Infection in the Rice Blast Pathogen Magnaporthe oryzae
Teng Fu, Gi-Chang Park, oon Hee Han, Jong-Hwan Shin, Hyun-Hoo Park, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 3 564-574 (11 pages)
Like many fungal pathogens, the conidium and appressorium play key roles during polycyclic dissemination and infection of Magnaporthe oryzae. Ran-binding protein microtubule-organizing center (RanBPM) is a highly conserved nucleocytoplasmic protein. In animalia, RanBPM has been implicated in apoptosis, cell morphology, and transcription. However, the functional roles of RanBPM, encoded by MGG_00753 (named MoRBP9) in M. oryzae, have not been elucidated. Here, the deletion mutant ΔMorbp9 for... -
vfr, A Global Regulatory Gene, is Required for Pyrrolnitrin but not for Phenazine-1-carboxylic Acid Biosynthesis in Pseudomonas chlororaphis G05
Xia Wu, Xiaoyan Chi, Yanhua Wang, Kailu Zhang, Le Kai, Qiuning He, Jinxiu Tang, Kewen Wang, Longshuo Sun, Xiuying Hao, Weihai Xie, Yihe Ge 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 4호 7 351-361 (11 pages)
a white conjugant G05W02 while transposon mutagenesis was carried out between a fusion mutant G05ΔphzΔprn::lacZ and E. coli S17- 1 (pUT/mini-Tn5Kan). By cloning and sequencing of the transposon-flanking DNA fragment, we found that a vfr gene in the conjugant G05W02 was disrupted with mini-Tn5Kan. In one other previous study on P. fluorescens, however, it was reported that the deletion of the vfr caused increased production of pyrrolnitrin and other antifungal metabolites. To confirm its... -
A Small GTPase RHO2 Plays an Important Role in Pre-infection Development in the Rice Blast Pathogen Magnaporthe oryzae
Teng Fu, Joon-Oh Kim, Joon-Hee Han, Adiyantara Gumilang, Yong-Hwan Lee, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 2 470-479 (10 pages)
to a hydrophobic surface when compared to the wild-type progenitor. This result indicates that MoRHO2 plays a role in appressorium development. Furthermore, exogenous cAMP treatment on the mutant led to appressoria that exhibited abnormal morphology on both hydrophobic and hydrophilic surfaces. These outcomes suggested the involvement of MoRHO2 in cAMP-mediated appressorium development. ΔMorho2 mutation also delayed the development of appressorium-like structures (ALS) at hyphal tips on... -
A Rice Gene Homologous to Arabidopsis AGD2-LIKE DEFENSE1 Participates in Disease Resistance Response against Infection with Magnaporthe oryzae
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 4호 9 357-362 (6 pages)
systemic resistance against bacterial infection. As well ALD1 is involved in the production of pipecolic acid and an unidentified compound(s) for systemic resistance and priming syndrome, respectively. These previous studies proposed that ALD1 is a potential candidate for developing genetically modified (GM) plants that may be resistant to pathogen infection. Here we introduce a role of ALD1-LIKE gene of Oryza sativa, named as OsALD1, during plant immunity. OsALD1 mRNA was strongly transcribed... -
Construction of a High-Quality Yeast Two-Hybrid Library and Its Application in Identification of Interacting Proteins with Brn1 in Curvularia lunata
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 2 108-114 (7 pages)
Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated. An yeast two-hybrid (Y2H) library is a very useful platform to further unravel novel pathogenic factors in C. lunata. To construct a high-quality full length-expression cDNA library from the C. lunata for application to pathogenesis-related protein-protein interaction screening, total RNA was extracted. The SMART (Switching... -
A Putative Transcription Factor pcs1 Positively Regulates Both Conidiation and Sexual Reproduction in the Cereal Pathogen Fusarium graminearum
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 3호 1 236-244 (9 pages)
in a dramatic reduction in conidial production and a complete loss of sexual reproduction. The pathways and gene ontology of pcs1-dependent genes from microarray experiments showed that several G-protein related pathways, oxidase activity, ribosome biogenesis, and RNA binding and processing were highly enriched, suggesting that pcs1 is involved in several different biological processes. Further, overexpression of pcs1 increased conidial production and resulted in earlier maturation of ascospores... -
Research Notes : Estrogenic Compounds Compatible with a Conditional Gene Expression System for the Phytopathogenic Fungus Fusarium graminearum
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 7 349-353 (5 pages)
a conditional gene expression system for this fungus using a ZEA-inducible promoter (Pzear). In the present study, four other estrogenic compounds, including β-estradiol, estriol, estrone, and secoisolariciresinol, were screened as possible substitutes for ZEA in this system. Among them, β-estradiol was able to successfully induce the expression of a gene controlled by Pzear, while estrone was only able to partially induce its expression but the other two compounds were not effective. In... -
A Short-chain Dehydrogenase/reductase Gene is Required for Infection-related Development and Pathogenicity in Magnaporthe oryzae
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 2 8-16 (9 pages)
as MoSDR1T-DNA and MoSDR1, respectively. Like other SDR family members, MoSDR1 possesses both a cofactor- binding motif and a catalytic site. The expression pattern of MoSDR1 suggests that the gene is associated with pathogenicity and plays an important role in M. oryzae development. To understand the roles of MoSDR1, the deletion mutant ΔMosdr1 for the gene was obtained via homology-dependent gene replacement. As expected, ΔMosdr1 was nonpathogenic; moreover, the mutant displayed pleiotropic... -
Expression of hpa1 Gene Encoding a Bacterial Harpin Protein in Xanthomonas oryzae pv. oryzae Enhances Disease Resistance to Both Fungal and Bacterial Pathogens in Rice and Arabidopsis
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 4호 3 364-372 (9 pages)
Xanthomonas oryzae pv. oryzae causing bacterial leaf blight disease in rice produces and secretes Hpa1 protein that belongs to harpin protein family. Previously it was reported that Hpa1 induced defense responses when it was produced in tobacco. In this study, we expressed hpa1 gene in rice and Arabidopsis to examine the effects of Hpa1 expression on disease resistance to both fungal and bacterial pathogens. Expression of hpa1 gene in rice enhanced disease resistance to both X. oryzae pv. oryzae... -
The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain
Eliana Guedes Stehling, *, Tatiana Amabile Campos, Marcelo Brocchi, Vasco Ariston de Carvalho Azevedo, Wanderley Dias da Silveira 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 10 75-83 (9 pages)
could adhere to and invade HEp-2 and tracheal epithelial cells, expressed fimbriae (observed by electron microscopy), and had five plasmids of 2.7, 4.7, 43, 56, and 88 MDa. Transposon mutagenesis of strain SEPT13, with transposon TnphoA, resulted in a mutant strain named ST16 that had a LD50 of 1.2 × 1012 CFU/ml. All other biological characteristics of strain ST16 were the same as those detected for strain SEPT13 except for the migration of an 88 MDa plasmid to the 93 MDa position indicatin... -
Carbamoyl Phosphate Synthase Subunit CgCPS1 Is Necessary for Virulence and to Regulate Stress Tolerance in Colletotrichum gloeosporioides
Aamar Mushtaq, Muhammad Tariq, Maqsood Ahmed, Zongshan Zhou, Imran Ali, Raja Tahir Mahmood 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 3 232-242 (11 pages)
a severe infectious dis- ease of apple whose infective area is growing gradually and thus poses a huge economic threat to the world. Different species of Colletotrichum including Colleto- trichum gloeosporioides are responsible for GLS. For efficient GLS control, it is important to understand the mechanism by which the cruciferous crops and C. gloeosporioides interact. Arginine is among one of the several types of amino acids, which plays crucial role in biochemical and physiological functions... -
Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides
Ping Wang, Bing Li, Yu-Ting Pan, Yun-Zhao Zhang, De-Wei Li, Lin Huang 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 1 385-397 (13 pages)
The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C2H2 TF CgCrzA is localized in both cytoplasm and nucleus under standard condition, and it translocated from cytoplasm to nucleus in a calcineurin- dependent manner. Moreover, the ΔCgCrzA was hypersensitive to cell wall... -
Antibody-Mediated Resistance to Rhizomania Disease in Sugar Beet Hairy Roots
M. Jafarzade, M. Ramezani, F. Hedayati, Z. Mokhtarzade, B. Zare, M. S. Sabet, P. Norouzi, M. A. Malboobi 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 15 692-697 (6 pages)
to a major coat protein of virus, p21, was targeted to various cellular compartments including the cytosol (pIC and pICC constructs), apoplast (pIA), and mitochondrion (pIM). After mechanical virus inoculation, most of the hairy root clones expressing scFv in the cytosol displayed low virus titers while the majority of transgenic hairy root clones accumulated antibody in outer membrane of mitochondria or apoplast were infected. This hairy root system provided an efficient and rapid approach to... -
Optimization of Polyethylene Glycol-Mediated Transformation of the Pepper Anthracnose Pathogen Colletotrichum scovillei to Develop an Applied Genomics Approach
Jong-Hwan Shin, Joon-Hee Han, Hyun-Hoo Park, Teng Fu, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 4 575-584 (10 pages)
transformation of KC05 using 19 different loci found throughout 10 (of 27) scaffolds, covering approximately 84.1% of the entire genome. PCR screening showed that the average transformation efficiency was about 17.1% per 100 colonies. Southern blot analyses revealed that at least one transformant per locus had single copy integration of PCR-screened positive transformants. Our results provide valuable information for a functional genomics approach to the pepper anthracnose pathogen C. scovillei. -
Visualization of Phytophthora palmivora Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers
Juan C. Ochoa, Mariana Herrera, M?nica Navia, Hern?n Mauricio Romero 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 3 19-31 (13 pages)
Bud rot (BR) is the most devastating disease affecting oil palm (Elaeis guineensis) crops in Colombia. Its causal agent, Phytophthora palmivora, initiates the infection in immature oil palm leaflets producing necrotic lesions, followed by colonization of opportunistic necrotrophs, which increases disease damage. To improve the characterization of the disease, we transformed P. palmivora using Agrobacterium tumefaciens-mediated transformation (ATMT) to include the fluorescent proteins CFPSKL... -
The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae
Jieun Kim, Mohamed Mannaa, Namgyu Kim, Chaeyeong Lee, Juyun Kim, Jungwook Park, Hyun-Hee Lee, Young-Su Seo 한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 5호 7 412-425 (14 pages)
a global small RNA chaperone that interacts with regulatory bacterial small RNAs (sRNA) and plays a role in the post-transcriptional regulation of gene expression. The roles of Hfq in the virulence and pathogenicity of several infectious bacteria have been reported. This study was conducted to elucidate the functions of two hfq genes in Burkholderia glumae, a causal agent of rice grain rot. Therefore, mutant strains of the rice-pathogenic B. glumae BGR1, targeting each of the two hfq genes, as... -
Expression of Rice Chitinase Gene in Genetically Engineered Tomato Confers Enhanced Resistance to Fusarium Wilt and Early Blight
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 6 252-258 (7 pages)
and TL3 of tomato Solanum lycopersicum Mill. cv. Riogrande genetically engineered with rice chitinase (RCG 3) gene and their R1 progeny was tested for resistance to Fol by root dip method and A. solani by detached leaf assay. All the R0 transgenic lines were highly resistant to these fungal pathogens compared to non-transgenic control plants. The pattern of segregation of three independent transformant for Fol and A. solani was also studied. Mendelian segregation was observed in transgenic lines... -
Roles of Ascospores and Arthroconidia of Xylogone ganodermophthora in Development of Yellow Rot in Cultivated Mushroom, Ganoderma lucidum
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 6 138-147 (10 pages)
constructed a green fluorescent proteinlabeled transgenic strain. This X. ganodermophthora strain produced a number of ascomata in the tissues of oak logs on which G. lucidum had been grown and on the mushroom fruit bodies. However, the ascospores released from the ascomata were not able to germinate on water agar or potato dextrose agar. Moreover, less than 0.1% of the ascospores showed green fluorescence, indicating that most ascospores of X. ganodermophthora were not viable. To determine the... -
GzRUM1, Encoding an Ortholog of Human Retinoblastoma Binding Protein 2, is Required for Ascospore Development in Gibberella zeae
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 4 20-25 (6 pages)
Gibberella zeae (anamorph: Fusarium graminearum), a homothallic (self-ferile) ascomycete with ubiquitous geographic distribution, causes serious diseases in several cereal crops. Ascospores (sexual spores) produced by this fungal pathogen have been suggested as the main source of primary inoculum in disease development. Here, we report the function of a gene designated GzRUM1, which is essential for ascospore formation in G. zeae. The deduced product of GzRUM1 showed significant similarities to... -
이 연구는 지난 세기의 근대 철학 비판을 계기로 성립한 바디우의 진리철 학의 윤곽을 제시하고, 그 철학적 함의를 드러내는 것을 목적으로 한다. 바디우의 진리철학은 존재론에서 시작한다. 그는 존재를 일자가 아닌 다수로 파악하면서 존 재를 비일관적 다수로 정의하고, 일자를 단지 비일관적 다수에 일관성을 부여하기 위한 작용으로 파악한다. 그는 집합론을 통해 존재론을 재구축하고, 존재의 질서에 공백이 편재한다는 것을 보여줌으로써 사건의 가능성을 존재의 질서 자체에 내재하 는 것으로 간주한다. 사건은 이러한 공백의...
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phbC 유전자가 도입된 형질전환 Alcaligenes eutrophus를 이용한 고분율 4-hydroxybutyrate 함유 P(3-hydroxybutyrate-4-hydroxybutyrate)의 생산
강명신, 정영미, 이용현, Gang. Myeong-Sin, Jeong. Yeong-Mi, Lee. Yong-Hyeon 한국생물공학회 한국생물공학회지 7 Pages
한국생물공학회 한국생물공학회지 1999, Vol.14 No.4 422-428 (7 pages)
phbC 유전자를 A. eutrophus에 재도입시킨 형질전환균주를 이용하여 높은 4HB 몰분율을 갖는 P(3HB-4HB)의 고농도 생산을 시도하였다. 형질전환균주는 총균체량, P(3HB-4HB) 농도, 그리고 축적률에서 모균주에 비해 다소 증가한 반면 P(3HB-4HB)내의 4HB 몰분율은 23.5 mol%로 모균주의 12.3 mol%에 비해 현저히 증가하였다. 이는 phbC유전자의 증폭으로 인해 해당과정에서 생성된 3HB와 전구물질인 ${gamma}$-butyrolacton에서 전환된 4HB의 중합반응이 촉진되기 때문으로 사료된다. 또한 ${gamma}$-butyrolacton의 농도 $Mg^{2-}$ 이온,... -
P(3-hydroxybutyrate-3-hydroxyvalerate)의 생산을 위한 재조합 phbC 유전자를 형질전환시킨 Alcaligenes eutrophus의 배양조건 검토
권순일, 정영미, 이용현 한국미생물생명공학회 산업미생물학회지 8 Pages
한국미생물생명공학회 산업미생물학회지 1998, Vol.26 No.6 537-544 (8 pages)
형질전환균주를 전구물질인 valerate가 10.0g/L첨가된 최소배지에서 2단계 배양한 결과 P(3HB-3HV)내의 3-HV 몰분율이 52.2 mol%로 모균주 A. eutrophus H16의 30 mol%에 비해 현저히 증가하였다. 이와 같은 몰분율의 증가는 phbC 유전자의 산물인 PHB synthase의 증폭과 밀접한 관련이 있음을 확인하였다. 2단계 회분배양시 fructose의 보충첨가의 영향을 검토하였으며, 적정농도인 10.0g/L의 fructose를 valerate와 같이 첨가할 경우 총균체량, p(3HB-3HV)의 축적농도, 그리고 축적율은 현저히 증가한 반면, 3-HV 몰분율은 다소 감소... -
Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV)in transgenic potato and its immunogenicity in mice
Dong Joo Ahn , Jung Won Youm , Suk Weon Kim , Won Kee Yoon , Hyoung Chin Kim , Tai Young Hur , Young Hee Joung , Jae Heu 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2013, 제 53권 제 4호 5 217-224 (8 pages)
