자료유형
등재정보
전체 선택해제
총 27건 검색
본문검색 결과만 보기
-
Intracellular calcium-dependent regulation of the sperm-specific calcium-activated potassium channel, hSlo3, by the BKCa activator LDD175
TharakaDarshanaWijerathne, JihyunKim, DongkiYang, KyuPilLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.2 12 241-249 (9 pages)
LRRC52 (leucine-rich-repeat–containing 52), is known to mediate the pHsensitive, sperm-specific K+ current KSper in mice, the molecular identity of this channel in human sperm remains controversial. In this study, we tested the classical BKCa activators, NS1619 and LDD175, on human Slo3, heterologously expressed in HEK293 cells together with its functional interacting γ2 subunit, hLRRC52. As previously reported, Slo3 K+ current was unaffected by iberiotoxin or 4-aminopyridine, but was... -
Acute Toxicity of the BKCa Channel Opener LDD175
Choi. Ji-Young, Choi. Jong-Hyun, Lee. Geum-Seon, Ko. Hong-Sook, Park. Chul-Seung, Kim. Yong-Chul, Cheong. Jae-Hoon 한국응용약물학회 The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmaco 6 Pages
한국응용약물학회 The journal of applied pharmacology : the official journal of the Korean Society of Applied Pharmaco 2006, Vol.14 No.4 253-258 (6 pages)
-
4-Aminopyridine Inhibits the Large-conductance Ca2+-activated K+ Channel (BKCa) Currents in Rabbit Pulmonary Arterial Smooth Muscle Cells
YoungMinBae, AeranKim, BokyungKim, Sung-IlCho, JunghwanKim, YungEEarm 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.1 5 25-28 (4 pages)
of BKCa. The BKCa currents were recorded in single channel resolution under the cell-attached mode of patch-clamp technique for minimal perturbation of intracellular environment. Application of 4-AP also inhibited the single BKCa currents reversibly and dose-dependently. The membrane potential of rabbit pulmonary arterial smooth muscle cells showed spontaneous transient hyperpolarizations (STHPs), presumably due to the STOC activities, which was also inhibited by 4-AP. These results suggest that... -
Inhibition of voltage-dependent K+ current in rabbit coronary arterial smooth muscle cells by the class Ic antiarrhythmic drug propafenone
Jin Ryeol An, Hongliang Li, Mi Seon Seo, Won Sun Park 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.5 13 597-605 (9 pages)
In this study, we demonstrated the inhibitory effect of the Class Ic antiarrhythmic agent propafenone on voltage-dependent K+ (Kv) channels using freshly isolated coronary artery smooth muscle cells from rabbits. The Kv current amplitude was progressively inhibited by propafenone in a dose-dependent manner, with an apparent IC50 value of 5.04±1.05 μM and a Hill coefficient of 0.78±0.06. The application of propafenone had no significant effect on the steady-state activation and inactivation... -
Taurine relaxes human radial artery through potassium channel opening action
Kemal Gokhan Ulusoy, Erkan Kaya, Kubilay Karabacak, Melik Seyrek, ?brahim Duvan, Vedat Yildirim, Oguzhan Yildiz 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.6 6 617-623 (7 pages)
The vascular actions and mechanisms of taurine were investigated in the isolated human radial artery (RA). RA rings were suspended in isolated organ baths and tension was recorded isometrically. First, a precontraction was achieved by adding potassium chloride (KCl, 45 mM) or serotonin (5-hydroxytryptamine, 5-HT, 30 µM) to organ baths. When the precontractions were stable, taurine (20, 40, 80 mM) was added cumulatively. Antagonistic effect of taurine on calcium chloride (10 µM to 10... -
Escitalopram, a selective serotonin reuptake inhibitor, inhibits voltage-dependent K+ channels in coronary arterial smooth muscle cells
HanSolKim, HongliangLi, HyeWonKim, SungEunShin, MiSeonSeo, JinRyeolAn, Kwon-SooHa, Eun-TaekHan, Seok-HoHong, Il-WhanChoi, GraceChoi, Dae-su 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.4 7 415-421 (7 pages)
We investigated the inhibitory effect of escitalopram, a selective serotonin reuptake inhibitor (SSRI), on voltage-dependent K+ (Kv) channels in freshly separated from rabbit coronary arterial smooth muscle cells. The application of escitalopram rapidly inhibited vascular Kv channels. Kv currents were progressively inhibited by an increase in the concentrations of escitalopram, suggesting that escitalopram inhibited vascular Kv currents in a concentration-dependent manner. The IC50 value and... -
Nortriptyline, a tricyclic antidepressant, inhibits voltage-dependent K+ channels in coronary arterial smooth muscle cells
SungEunShin, HongliangLi, HanSolKim, HyeWonKim, MiSeonSeo, Kwon-SooHa, Eun-TaekHan, Seok-HoHong, AmyL.Firth, Il-WhanChoi6, YoungMinBae, Won 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.2 10 225-232 (8 pages)
We demonstrated the effect of nortriptyline, a tricyclic antidepressant drug and serotonin reuptake inhibitor, on voltage-dependent K+ (Kv) channels in freshly isolated rabbit coronary arterial smooth muscle cells using a whole-cell patch clamp technique. Nortriptyline inhibited Kv currents in a concentration-dependent manner, with an apparent IC50 value of 2.86±0.52 µM and a Hill coefficient of 0.77±0.1. Although application of nortriptyline did not change the activation curve,... -
Effects of hydrogen peroxide on voltage-dependent K+ currents in human cardiac fibroblasts through protein kinase pathways
HyemiBae, DongheeLee, Young-WonKim, JeongyoonChoi, HongJunLee, Sang-WookKim, TaehoKim, Yun-HeeNoh, Jae-HongKo, HyoweonBang, InjaLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.3 11 315-324 (10 pages)
of KCa channels were detected in HCFs: BKCa channels, small-conductance KCa (SKCa) channels, and intermediate-conductance KCa (IKCa) channels. In the annexin V/propidium iodide assay, apoptotic changes in HCFs increased in response to H2O2, but IbTX decreased H2O2-induced apoptosis. These data suggest that among the VDKCs of HCFs, H2O2 only enhances BKCa currents through the protein kinase G pathway but not the protein kinase A pathway, and is involved in cell injury through BKCa channels. -
Activation of K+ channel by 1-EBIO rescues the head and neck squamous cell carcinoma cells from Ca2+ ionophore-induced cell death
MingZheYin, Seok-WooPark, , TaeWookKang, KyungSooKim, , , HaeYoungYoo6, JunhoLee, J.HunHah, , MyungHunSung, , SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.1 4 25-33 (9 pages)
Ion channels in carcinoma and their roles in cell proliferation are drawing attention. Intracellular Ca2+ ([Ca2+]i)-dependent signaling affects the fate of cancer cells. Here we investigate the role of Ca2+-activated K+ channel (SK4) in head and neck squamous cell carcinoma cells (HNSCCs) of dif-ferent cell lines; SNU-1076, OSC-19 and HN5. Treatment with 1 μM ionomycin induced cell death in all the three cell lines. Whole-cell patch clamp study suggested common expressions of Ca2+-activated... -
Activation of Lysophosphatidic Acid Receptor Is Coupled to Enhancement of Ca2＋-Activated Potassium Channel Currents
Sun-HyeChoi, Byung-HwanLee, Hyeon-JoongKim, Sung-HeeHwang, Sang-MokLee, Seung-YeolNah 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.3 7 223-228 (6 pages)
The calcium-activated K＋ (BKCa) channel is one of the potassium-selective ion channels that are present in the nervous and vascular systems. Ca2＋ is the main regulator of BKCa channel activation. The BKCa channel contains two high affinity Ca2＋ binding sites, namely, regulators of K＋ conductance, RCK1 and the Ca2＋ bowl. Lysophosphatidic acid (LPA, 1-radyl-2-hydroxy-sn-glycero-3-phosphate) is one of the neurolipids. LPA affects diverse cellular functions on many cell types through G protein-... -
Contradictory Effects of Superoxide and Hydrogen Peroxide on KCa3.1 in Human Endothelial Cells
ShinkyuChoi, Hye-YoungNa, JiAeeKim, Sung-EunCho, SukHyoSuh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2013, Vol.17 No.3 1 181-187 (7 pages)
Reactive oxygen species (ROS) are generated in various cells, including vascular smooth muscle and endothelial cells, and regulate ion channel functions. KCa3.1 plays an important role in endothelial functions. However, the effects of superoxide and hydrogen peroxide radicals on the expression of this ion channel in the endothelium remain unclear. In this study, we examined the effects of ROS donors on KCa3.1 expression and the K＋ current in primary cultured human umbilical vein endothelial... -
Activation of the cGMP/Protein Kinase G Pathway by Nitric Oxide Can Decrease TRPV1 Activity in Cultured Rat Dorsal Root Ganglion Neurons
YunjuJin, JunKim, JiyeonKwak 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.3 10 211-217 (7 pages)
Recent studies have demonstrated that nitric oxide (NO) activates transient receptor potential vanilloid subtype 1 (TRPV1) via S-nitrosylation of the channel protein. NO also modulates various cellular functions via activation of the soluble guanylyl cyclase (sGC)/protein kinase G (PKG) pathway and the direct modification of proteins. Thus, in the present study, we investigated whether NO could indirectly modulate the activity of TRPV1 via a cGMP/PKG-dependent pathway in cultured rat dorsal root... -
Bile Acid Inhibition of N-type Calcium Channel Currents from Sympathetic Ganglion Neurons
HyeKyungLee, KyoungHwaLee, Eui-SicCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2012, Vol.16 No.1 4 25-30 (6 pages)
Under some pathological conditions as bile flow obstruction or liver diseases with the enterohepatic circulation being disrupted, regurgitation of bile acids into the systemic circulation occurs and the plasma level of bile acids increases. Bile acids in circulation may affect the nervous system. We examined this possibility by studying the effects of bile acids on gating of neuronal (N)-type Ca2＋ channel that is essential for neurotransmitter release at synapses of the peripheral and central... -
Quercetin-induced Growth Inhibition in Human Bladder Cancer Cells Is Associated with an Increase in Ca2＋-activated K＋ Channels
YangmiKim, Wun-JaeKim, , Eun-JongCha 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.5 5 279-284 (6 pages)
non-specific BKCa blocker and iberiotoxin (IBX; 100 nM) a BKCa-specific blocker. Quercetin-induced membrane hyper</SUB>polarization was measured by fluorescence-activated cell sorting (FACS) with voltage sensitive dye, bis (1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3); 100 nM). Quercetin-evoked hyperpolarization was prevented by TEA. Quercetin produced an antiproliferative effect (30.3±13.5%) which was recovered to 53.3±10.5% and 72.9±3.7% by TEA and IBX, respectively. Taken... -
Possible Involvement of Ca2＋ Activated K＋ Channels, SK Channel, in the Quercetin-Induced Vasodilatation
SeiichiroNishida, HiroyasuSatoh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.5 5 361-365 (5 pages)
Effects of quercetin, a kind of flavonoids, on the vasodilating actions were investigated. Among the mechanisms for quercetin-induced vasodilatation in rat aorta, the involvement with the Ca2＋ activated K＋ (KCa) channel was examined. Pretreatment with NE (5ՌM) or KCl (60 mM) was carried out and then, the modulation by quercetin of the constriction was examined using rat aorta ring strips (3 mm) at 36.5oC. Quercetin (0.1 to 100ՌM) relaxed the NE-induced vasoconstrictions in a... -
Inhibition of Arterial Myogenic Responses by a Mixed Aqueous Extract of Salvia Miltiorrhiza and Panax Notoginseng (PASEL) Showing Antihypertensive Effects
EunBokBaek, HaeYoungYoo, SuJungPark, Young-ShinChung, Eun-KyungHong, SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.4 4 287-293 (7 pages)
The dried roots of Danshen (Salvia miltiorrhiza) and Sanchi (Panax notoginseng) have been widely used in traditional Chinese medicine for promoting blood circulation as well as various other bodily functions. Here we investigated the effects of a mixture of aqueous extracts of Danshen and Sanchi, named PASEL, on blood pressure and vascular contractility in rats. Orally administered PASEL (62.5 mg/kg and 250 mg/kg, for 5 weeks) lowered the blood pressure of spontaneous hypertensive rats (SHR) but... -
Oxidized Low-density Lipoprotein- and Lysophosphatidylcholine-induced Ca2＋ Mobilization in Human Endothelial Cells
MoonYoungKim, GuoHuaLiang, JiAeeKim, SooSeungChoi, ShinkuChoi, SukHyoSuh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.1 5 27-32 (6 pages)
presence of tempol. Furthermore, in voltage-clamped HUVECs, OxLDL enhanced SOC and evoked outward currents simultaneously. Clamping intracellular Ca2＋ to 1 ՌM activated large-conductance Ca2＋-activated K＋ (BKCa) current spontaneously, and this activated BKCa current was further enhanced by OxLDL or by LPC. From these results, we concluded that OxLDL or its main component LPC activates Ca2＋-permeable Ca2＋-activated NSC current and BKCa current simultaneously, thereby increasing SOC. -
Mechanical Hyperalgesia Induced by Blocking Calcium-activated Potassium Channels on Capsaicin-sensitive Afferent Fiber
KyungHeeLee, HongKeeShin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2007, Vol.11 No.5 8 215-219 (5 pages)
Small and large conductance Ca2+-activated K+ (SKCa and BKCa) channels are implicated in the modulation of neuronal excitability. We investigated how changes in peripheral KCa channel activity affect mechanical sensitivity as well as the afferent fiber type responsible for KCa channel-induced mechanical sensitivity. Blockade of SKCa and BKCa channels induced a sustained decrease of mechanical threshold which was significantly attenuated by topical application of capsaicin onto afferent fiber and... -
Influence of Nicorandil on Catecholamine Release in the Perfused Rat Adrenal Medulla
Young-YoupKoh, Eun-SookLee, Hae-JeongNo, , Seong-ChangWoo, Joong-WhaChung, Yoo-SeungSeoh, Dong-YoonLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2007, Vol.11 No.3 4 97-106 (10 pages)
The present study was attempted to investigate the effect of nicorandil, which is an ATP-sensitive potassium (KATP) channel opener, on secretion of catecholamines (CA) evoked by cholinergic stimulation and membrane depolarization from the isolated perfused rat adrenal glands. The perfusion of nicorandil (0.3∼3.0 mM) into an adrenal vein for 90 min produced relatively dose-and time-dependent inhibition in CA secretion evoked by ACh (5.32 mM), high K+ (a direct membrane depolarizer, 56 mM), DMPP... -
Modulation of Large Conductance Ca2+-activated K+ Channel of Skin Fibroblast (CRL-1474) by Cyclic Nucleotides
JihyunYun, SeungtaeKim, HyoweonBang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.2 9 131-134 (4 pages)
potential and [Ca2]i, consistent with the known properties of BKCa channels. BKCa channels in CRL-1474 were positively regulated by adenylate cyclase activator (10μM forskolin), 8-Br-cyclic AMP (300μM) or 8-Br-cyclic GMP (300μM). These results suggest that human skin fibroblasts (CR-1474) have typical BKCa channel and this channel could be modulated by c-AMP and c-GMP. The electrophysiological characteristics of fibroblasts might be used as the diagnostic clues for Alzheimer disease. -
Intrinsic Gating in Inward Rectifier Potassium Channels (Kir2.1) with Low Polyamine Affinity Generated by Site Directed Mutagenesis
ISo, , IAshmole, HSoh, CSPark, PJSpencer, MLeyl, PRStanfield, 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 3 131-142 (12 pages)
We have studied mutant forms of Kir2.1 in which an aspartate residue (D172), important for gating by intracellular polyamines, is replaced by one of three basic residues (Arg, Lys or His). Such channels are highly selective for K+, but show inward rectification that is a shallow function of voltage compared with that found in wild type. This inward rectification occurs with a reduced affinity for spermine and persists in the absence of polyamines. Though the unitary current-voltage relation... -
Roles of Ca2+-Activated K+ Conductances on Spontaneous Firing Patterns of Isolated Rat Medial Vestibular Nucleus Neurons
SangWooChun, JaeWooJun, ByungRimPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.1 1 1-8 (8 pages)
To investigate the contributions of intrinsic membrane properties to the spontaneous activity of medial vestibular nucleus (MVN) neurons, we assessed the effects of blocking large and small calcium-activated potassium channels by means of patch clamp recordings. Almost all the MVN neurons recorded in neonatal (P13∼P17) rat were shown to have either a single deep after-hyperpolarization (AHP; type A cells), or an early fast and a delayed slow AHP (type B cells). Among the recorded MVN cells,... -
Ca2+ Signalling in Endothelial Cells Role of Ion Channels
BerndNilius, FelixViana, MasahiroKamouchi, CristinaFasolato, JanEggermont, GuyDroogmans 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 13 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.2 1 133-145 (13 pages)
-
Two Types of Voltage-dependent Outward Potassium Currents in Smooth Muscle Cells of Rabbit Basilar Atery
TongMookKang, InsukSo, DaeYongUhm, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 15 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.2 7 169-183 (15 pages)
We have investigated the two types of voltage-dependent outward potassium (K) currents, i.e. delayed rectifier K current (IK(V)) and 'A-like' transient outward K current (Ito) with patch-clamp technique in single smooth muscle cells (SMCs) isolated from rabbit basilar artery, and investigated the characteristics of them. The time-courses of activation were well fitted by exponential function raised to second power (n2) in IK(v) and fourth power (n4) in Ito. The activation, inactivation and... -
Effects of Lemakalim, a Potassium Channel Opener, on the Contractility and Electrical Activity of the Antral Circular Muscle in Guinea-Pig Stomach
Kim, Sung-Joon, Jun, Jae-Yeoul, Choi, Youn-Baik, Kim, Ki-Whan, Kim, Woo-Gyeum 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 1호 4 37-50 (14 pages)
glibenclamide and a high concentration of intracelIular EGTA (10 mM). Volatage-gated Ca currents were not affected by lemakalim. In inside-out patch clamp experiments, lemakalim increased the opening frequency of the large conductance Ca2+-activated K channels (BKCa, Maxi-K). From these results, it is suggested that lemakalim induces hyperpolarization by opening K-channels which are sensitive to internal Ca and such a hyperpolarization leads to the inhibition of the spontaneous contraction. -
Immunohistochemical study on the distribution of ion channels in rat trigeminal sensory nucleus
박호영, 최기운, 최호영, Choi. Ho-Young, Choi. Gi-Woon, Choi. Ho-Young 대한치과보존학회 大韓齒科保存學會誌 17 Pages
대한치과보존학회 大韓齒科保存學會誌 2002, Vol.27 No.3 215-231 (17 pages)
rectifying 포타슘 통로(Kir 2.1)가 중등도로 염색되었고, voltage-gated 포타슘 통로(Kv 4.2)가 약하게 염색되었으며, BKCa는 그 염색 정도가 매우 약하게 나타났다. 이상의 결과를 종합해 볼 때 삼차신경 감각핵에는 소디움 통로의 분포가 가장 많았으며, 칼슘통로에서는 N-type이, 포타슘 통로 중에는 inwardly rectifying 통로(Kir 2.1)가 가장 많이 분포함을 관찰할 수 있었다. 것이 관찰되었다 (p＜0.05). 경화 후 Roth 801, 경화전과 후의 Dentalis, AH plus, Apexit, RSA는 E. faecalis에 대한 항균효과를 나타내지 못하였다. S....
