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Estimation of the Substrate Size with Minimum Mutual Coupling of a Linear Microstrip Patch Antenna Array Positioned Along the H-Plane
Kwak. Eun-Hyuk, Yoon. Young-Min, Kim. Boo-Gyoun 대한전기학회 Journal of electrical engineering & technology 5 Pages
대한전기학회 Journal of electrical engineering & technology 2015, Vol.10 No.1 320-324 (5 pages)
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β-irradiation (Ho patch)-induced skin injury in mini-pigs: effects on NF-κB and COX-2 expression in the skin
Joong-Sun Kim, Kyung-Jin Rhim, Won-Seok Jang, Sun-Joo Lee, Yeonghoon Son, Seung-Sook Lee, Sunhoo Park, Sang Moo Lim 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 1 1-9 (9 pages)
In the present study, the detrimental effect of β-emission on pig skin was evaluated. Skin injury was modeled in mini-pigs by exposing the animals to 50 and 100 Gy of β-emission delivered by Ho patches. Clinicopathological and immunohistochemical changes in exposed skin were monitored for 18 weeks after β-irradiation. Radiation induced desquamation at 2∼4 weeks and gradual repair of this damage was evident 6 weeks after irradiation. Changes in basal cell density... -
A Study of the Mechanical Properties of Patch-Bonded and Riveted Repairs on Cracked Al 6061-T6 alloy Structures
Yoon. Young-Ki, Kim. Guk-Gi, Yoon. Hi-Seak 한국정밀공학회 International journal of the Korean society of precision engineering 6 Pages
한국정밀공학회 International journal of the Korean society of precision engineering 2000, Vol.1 No.2 55-60 (6 pages)
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Comparison of electrophysiological properties of two types of pre-sympathetic neurons intermingled in the hypothalamic paraventricular nucleus
Yiming Shen, Seong Kyu Han, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 2 483-491 (9 pages)
using patch-clamp and retrograde-tracing techniques. Most neurons (＞70%) in both groups spontaneously fired in the cell-attached mode. When compared to the PVNIML neurons, the PVNRVLM neurons had a lower firing rate and a more irregular firing pattern (p ＜ 0.05). The PVNRVLM neurons showed smaller resting membrane potential, slower rise and decay times, and greater duration of spontaneous action potentials (p ＜ 0.05). The PVNRVLM neurons received greater inhibitory synaptic inputs (frequency,... -
Molecular cloning and characterization of porcine ribosomal protein L21
Wu-Sheng Sun, Ju-Lan Chun, Dong-Hwan Kim, Jin-Seop Ahn, Min-Kyu Kim, In-Sul Hwang, Dae-Jin Kwon, Seongsoo Hwang, Jeong-Woong Lee 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 18 531-540 (10 pages)
Ribosomal protein L21 (RPL21) is a structural component of the 60S subunit of the eukaryotic ribosome. This protein has an important role in protein synthesis and the occurrence of hereditary diseases. Pig is a common laboratory model, however, to the best of our knowledge, its RPL21 gene has not been cloned to date. In this study, we cloned and identified the full-length sequence of the pig RPL21 gene for the first time. In addition, we examined its expression pattern and function by using... -
Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice
Yu-Ying Liu, Wen-Tao Yang, Shao-Hua Shi, Ya-Jie Li, Liang Zhao, Chun-Wei Shi, Fang-Yu Zhou, Yan-Long Jiang, Jing-Tao Hu, Wei Gu, 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 5 159-167 (9 pages)
Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus (L.) plantarum NC8 was selected as a vector to express the VP2 gene of GPV, and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 × 10... -
Therapeutic effect of topical application of curcumin during treatment of radiation burns in a mini-pig model
Joongsun Kim, Sunhoo Park, Byung-Suk Jeon, Won-Seok Jang, Sun-Joo Lee, Yeonghoon Son, Kyung-Jin Rhim, Soong In Lee, Seung-Sook L 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 1 435-444 (10 pages)
Curcumin protects the skin against radiation-induced epidermal damage and prevents morphological changes induced by irradiation skin, thereby maintaining the epidermal thickness and cell density of basal layers. In this study, the effects of topical curcumin treatment on radiation burns were evaluated in a mini-pig model. Histological and clinical changes were observed five weeks after radiation exposure to the back (Co gamma-radiation, 50 Gy). Curcumin was applied topically to irradiated skin... -
Chitosan hemostatic dressing for control of hemorrhage from femoral arterial puncture site in dogs
Viktor Szatm?ri 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 16 517-523 (7 pages)
Removal of an introducer-sheath from a femoral artery after completing transarterial embolization of a patent ductus arteriosus can cause life-threatening hemorrhage in dogs. In the present study, the effectiveness of chitosan acetate dressing in 10 experimental dogs was tested. Under general anesthesia, an introducer-sheath was placed into the femoral artery with percutaneous puncture using Seldinger’s technique. The outer diameter of the introducer-sheaths varied from 3.0 to 4.0 mm with an... -
Developmental changes in cell proliferation and apoptosis in the normal duck bursa of Fabricius
Jing Fang, Xi Peng 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 2 465-474 (10 pages)
The aim of this work was to investigate developmental changes in cell proliferation and apoptosis in normal duck bursa of Fabricius using flow cytometry and immunohistochemistry. Studies were carried out on Tianfu ducks on days 24 and 27 of embryogenesis (E24 and E27) along with days 20, 70, and 200 of postnatal development (P20, P70, and P200). Results showed that the percentage of G0/G1 bursa cells significantly increased between E24 and P200 while the percentage of cells in the S phase or G2... -
Laparoscopic left hepatectomy in swine: a safe and feasible technique
Hua Zhang, Tao Liu, Yue Wang, Hai-feng Liu, Jian-tao Zhang, Yan-shuang Wu, Lei Lei, Hong-bin Wang* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 10 417-422 (6 pages)
A purely laparoscopic four-port approach was created for left hepatectomy in pigs. A polyethylene loop was placed on the left two hepatic lobes for traction and lift. Next, penetrating ligation of the lobes using of a double row of silk sutures was performed to control bleeding. A direct hepatic transection was completed using a monopolar hook electrode without meticulous dissection of the left hepatic vein. The raw surface of the liver was coagulated and sealed with fibrin glue. Lobes were... -
C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches
Kana Tozaki, Junpei Kimura, Masahiro Yasuda*, Nobuyuki Ryu, Testuo Nasu, Anton Pernthaner, Wayne R. Hein 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 1 1-6 (6 pages)
The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous... -
Percutaneous heartworm removal from dogs with severe heart worm (Dirofilaria immitis) infestation
Seung-Gon Lee, Hyeong-Sun Moon, Changbaig Hyun* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 11 197-202 (6 pages)
Canine heart worm disease is often life-threatening due to its various complications, including right side heart failure, caval syndrome and pulmonary eosinophilic granulomatosis. Several preventive medications and melarsomine have been developed and they are very effective to control heartworm infestation. However, in a case of severe infestation, melarsomine therapy often results in an unfavorable outcome because of the severe immune reaction caused by rapid killing of the adult worm. Surgical... -
Comparison of different bronchial closure techniques following pneumonectomy in dogs
Hakan Salci*, A. Sami Bayram, Ozgur Ozyigit, Cengiz Gebitekin, O. Sacit Gorgul 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 12 393-400 (8 pages)
The comparison of the histologic healing and bronchopleural fistula (BPF) complications encountered with three different BS closure techniques (manual suture, stapler and manual suture plus tissue flab) after pneumonectomy in dogs was investigated for a one-month period. The dogs were separated into two groups: group I (GI) (n=9) and group II (GII) (n=9). Right and left pneumonectomies were performed on the animals in GI and GII, respectively. Each group was further divided into three subgroups... -
Intrahepatic portosystemic shunt with a second degree atrioventricular block fixed by transvenous coil embolization in a dog
Seung Gon Lee , So Jeong Nam , Hyun Wook Kim , Chang Baig Hyun , 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2008, 제 48권 제 4호 15 493-500 (8 pages)
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Tricuspid valve dysplasia complicated with pulmonic regurgitation in a Cocker Spaniel dog
So Jeong Nam , Ran Choi , In Chul Park , Chang Baig Hyun , 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2008, 제 48권 제 4호 13 481-487 (7 pages)
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Multicentric T cell Lymphoma in a Maltese dog
JiYoulJung, SangChulKang, InSoonRoh, HyunJooSohn, YoungMinYun, JungHunKim, KyoungKapLee, MinChanAn, JongHeeBae, JaeHoonKim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2007, 제 47권 제 1호 11 85-89 (5 pages)
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중서부 신석기시대 편년과 패총 이용전략에 대한 새로운 이해
金壯錫(Jangsuk Kim), 梁成赫(Seonghyeok Yang) 한국고고학회 한국고고학보 40 Pages
한국고고학회 한국고고학보 2001, 제 45집 1 5-44 (40 pages)
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인터랙티브 사운드 아트 구현을 위한 인터페이스 디자인 연구
박정권(ChungGwon PARK) 한국음악교육공학회 음악교육공학 27 Pages
한국음악교육공학회 음악교육공학 2012, 제 15호 12 209-235 (27 pages)
본 연구는 인터랙티브 아트 구현을 시도하려는 예술인들에게, 상용화된 고가의 장치 및 제어 소프트웨어의 도움 없이 작품 창작 전반에 걸친 과정을 자체적으로 수행 할 수 있는 방향을 제시함으로써 상업적인 도구에 의한 유사한 작품 창작활동으로부터 벗어나 예술인의 개성이 담긴 독창적인 창작 활동에 도움을 주고자 한다. 인터랙티브 아트를 위한 컴퓨터 프로그래밍 언어 도구의 정보 및 기본적인 사용법을 알아보고, 물리적 인터페이스의 제작에 어려움을 겪을 수 있는 음악인들에게 인터랙티브 작품 제작에 기본적으로... -
음식물 불내성에 대한 고찰
박정현(Jung Hyun Park), 김호준(Ho Jun Kim), 이명종(Myeong Jong Lee) 한방비만학회 대한한방비만학회지 12 Pages
한방비만학회 대한한방비만학회지 2008, 제 8권 제 1호 1 1-12 (12 pages)
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Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth
Dong Ho Bak, Mi Ji Choi, Soon Re Kim, Byung Chul Lee, Jae Min Kim, Eun Su Jeon, Wonil Oh, Ee Seok Lim, Byung Cheol Park, Moo Joong Kim, Ju 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.5 9 555-566 (12 pages)
Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair... -
Botulinum toxin type A enhances the inhibitory spontaneous postsynaptic currents on the substantia gelatinosa neurons of the subnucleus caudalis in immature mice
Seon-Hui Jang, Soo-Joung Park, Chang-Jin Lee, Dong-Kuk Ahn, Seong-Kyu Han 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.5 7 539-546 (8 pages)
Botulinum toxin type A (BoNT/A) has been used therapeutically for various conditions including dystonia, cerebral palsy, wrinkle, hyperhidrosis and pain control. The substantia gelatinosa (SG) neurons of the trigeminal subnucleus caudalis (Vc) receive orofacial nociceptive information from primary afferents and transmit the information to higher brain center. Although many studies have shown the analgesic effects of BoNT/A, the effects of BoNT/A at the central nervous system and the action... -
The advantage of topographic prominence-adopted filter for the detection of short-latency spikes of retinal ganglion cells
JungryulAhn, Myoung-HwanChoi, KwangsooKim, SolomonS.Senok, Dong-ilDanCho, Kyo-inKoo, YongsookGoo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.5 10 555-563 (9 pages)
Electrical stimulation through retinal prosthesis elicits both short and long-latency retinal ganglion cell (RGC) spikes. Because the short-latency RGC spike is usually obscured by electrical stimulus artifact, it is very important to isolate spike from stimulus artifact. Previously, we showed that topographic prominence (TP) discriminator based algorithm is valid and useful for artifact subtraction. In this study, we compared the performance of forward backward (FB) filter only vs. TP-adopted... -
DAMGO modulates two-pore domain K+ channels in the substantia gelatinosa neurons of rat spinal cord
PyungSunCho, HanKyuLee, , SangHoonLee, JayZoonIm, SungJunJung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.5 10 525-531 (7 pages)
a slice whole-cell patch clamp technique. Also we confirmed which subtypes of K2P channels were associated with DAMGO-induced currents, measuring the expression of K2P channel in whole spinal cord and SG region. DAMGO caused a robust hyperpolarization and outward current in the SG neurons, which developed almost instantaneously and did not show any time-dependent inactivation. Half ofthe SG neurons exhibited a linear I~V relationship of the DAMGO-induced current, whereas rest of the neurons... -
Effects of hydrogen peroxide on voltage-dependent K+ currents in human cardiac fibroblasts through protein kinase pathways
HyemiBae, DongheeLee, Young-WonKim, JeongyoonChoi, HongJunLee, Sang-WookKim, TaehoKim, Yun-HeeNoh, Jae-HongKo, HyoweonBang, InjaLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.3 11 315-324 (10 pages)
whether H2O2 could modulate VDKCs in HCFs and induce cell injury through this process. In whole-cell mode patch-clamp recordings, application of H2O2 stimulated Ca2+-activated K+ (KCa) currents but not delayed rectifier K+ or transient outward K+ currents, all of which are VDKCs. H2O2-stimulated KCa currents were blocked by iberiotoxin (IbTX, a large conductance KCa blocker). The H2O2-stimulating effect on large-conductance KCa (BKCa) currents was also blocked by KT5823 (a protein kinase G... -
Airway Smooth Muscle Sensitivity to Methacholine in Precision-Cut Lung Slices (PCLS) from Ovalbumin-induced Asthmatic Mice
HaeJinKim, YeryungKim, SuJungPark, BoramBae, Hye-RyunKang, Sang-HeonCho, HaeYoungYoo, JooHyunNam, WooKyungKim, SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.1 10 65-72 (8 pages)
Asthma is a chronic inflammatory disease characterized by airway hyperresponsiveness (AHR) and reversible airway obstruction. Methacholine (MCh) is widely used in broncho-provocation test to evaluate airway resistance. For experimental investigation, ovalbumin-induced sensitization is fre-quently used in rodents (Ova-asthma). However, albeit the inflammatory histology and AHR in vivo, it remains unclear whether the MCh sensitivity of airway smooth muscle isolated from Ova-asthma is persistently... -
Push-in Head Restraining Apparatus for Intracranial Self Stimulation Tasks in Rats
MootaekRoh, Il-SungJang, Maan-GeeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.2 3 103-108 (6 pages)
Head restraining is an experimental technique that firmly secures the animal’s head to a fixation apparatus for the precise control and sensing of behaviors. However, procedural and surgical difficulties and limitations have been obstructing the use of the technique in neurophysiological and behavioral experiments. Here, we propose a novel design of the head-restraining apparatus which is easy to develop and convenient for practical use. Head restraining procedure can be completed by sliding... -
The Pathophysiologic Roles of TRPM7 Channel
HyunSooPark, ChansikHong, ByungJooKim, InsukSo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.1 2 15-23 (9 pages)
Transient receptor potential melastatin 7 (TRPM7) is a member of the melastatin-related subfamily and contains a channel and a kinase domain. TRPM7 is known to be associated with cell proliferation, survival, and development. It is ubiquitously expressed, highly permeable to Mg2＋ and Ca2＋, and its channel activity is negatively regulated by free Mg2＋ and Mg-complexed nucleotides. Recent studies have investigated the relationships between TRPM7 and a number of diseases. TRPM7 regulates cell... -
Direct Corticosteroid Modulation of GABAergic Neurons in the Anterior Hypothalamic Area of GAD65-eGFP Mice
SeungYubShin, TaeHeeHan, SoYeongLee, SeongKyuHan, JinBongPark, FerencErdelyi, GaborSzabo, PanDongRyu 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.3 7 163-169 (7 pages)
Corticosterone is known to modulate GABAergic synaptic transmission in the hypothalamic paraventricular nucleus. However, the underlying receptor mechanisms are largely unknown. In the anterior hypothalamic area (AHA), the sympathoinhibitory center that project GABAergic neurons onto the PVN, we examined the expression of glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) of GABAergic neurons using intact GAD65-eGFP transgenic mice, and the effects of corticosterone on the burst... -
Regulation of Adenosine-activated GIRK Channels by Gq-coupled Receptors in Mouse Atrial Myocytes
HanaCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.3 5 145-150 (6 pages)
it is not known whether GIRK channels activated by Ado signaling are also regulated by GqPCRs. Presently, this was investigated in mouse atrial myocytes using the patch clamp technique. GIRK channels were activated by 100ՌM Ado. When Ado was repetitively applied at intervals of 5∼6 min, the amplitude of second Ado-activated GIRK currents (IK(Ado)) was 88.3±3.7% of the first IK(Ado) in the control. Pretreatment of atrial myocytes with phenylephrine, endothelin-1, or bradykinin prior to a... -
A Carbohydrate Fraction, AIP1, from Artemisia Iwayomogi Reduces the Action Potential Duration by Activation of Rapidly Activating Delayed Rectifier K＋ Channels in Rabbit Ventricular Myocytes
WonSunPark, YounKyoungSon, EunAKo, SeongWooChoi, NariKim, Tae-HoonChoi, HyunJooYoun, Su-HyunJo, DaHyeHong, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.3 1 119-125 (7 pages)
cardiac ventricular delayed rectifier K＋ current (IK) using the patch clamp technique. The carbohydrate fraction AIP1 dose-dependently increased the heart rate with an apparent EC50 value of 56.1±5.5Ռg/ml. Application of AIP1 reduced the action potential duration (APD) in concentration-dependent fashion by activating IK without significantly altering the resting membrane potential (IC50 value of APD50: 54.80±2.24, IC50 value of APD90: 57.45±3.47Ռg/ml). Based on the results, all... -
Modeling of Arrhythmogenic Automaticity Induced by Stretch in Rat Atrial Myocytes
JaeBoumYoum, ChaeHunLeem, YinHuaZhang, NariKim, JinHan, YungE.Earm 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.5 8 267-274 (8 pages)
Since first discovered in chick skeletal muscles, stretch-activated channels (SACs) have been proposed as a probable mechano-transducer of the mechanical stimulus at the cellular level. Channel properties have been studied in both the single-channel and the whole-cell level. There is growing evidence to indicate that major stretch-induced changes in electrical activity are mediated by activation of these channels. We aimed to investigate the mechanism of stretch-induced automaticity by... -
Single-Channel Recording of TASK-3-like K+ Channel and Up- Regulation of TASK-3 mRNA Expression after Spinal Cord Injury in Rat Dorsal Root Ganglion Neurons
InseokJang, Jun-HoLa, Gyu-TaeKim, Jeong-SoonLee, Eun-JinKim, Eun-ShinLee, Su-JeongKim, Jeong-MinSeo, Sang-HoAhn, Jae-YongPark, Seong-GeunH 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.5 5 245-252 (8 pages)
Single-channel recordings of TASK-1 and TASK-3, members of two-pore domain K+ channel family, have not yet been reported in dorsal root ganglion (DRG) neurons, even though their mRNA and activity in whole-cell currents have been detected in these neurons. Here, we report single-channel kinetics of the TASK-3-like K+ channel in DRG neurons and up-regulation of TASK-3 mRNA expression in tissues isolated from animals with spinal cord injury (SCI). In DRG neurons, the single-channel conductance of... -
Testosterone Relaxes Rabbit Seminal Vesicle by Calcium Channel Inhibition
JongKokKim, WooHaHan, MooYeolLee, SoonChulMyung, SaeChulKim, MinKyKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.2 6 73-78 (6 pages)
myocytes of rabbit were isolated using proteolytic enzymes including collagenase and papain. Inside-out, attached, and whole-cell configurations were examined using the patch clamp technique. The applications of 10ՌM norepinephrine or 70 mM KCl induced tonic contractions, and 10ՌM testosterone (pharmacological concentration) evoked dose-dependent relaxations of these precontracted strips. Various K+ channel blockers, such as tetraethylammonium (TEA; 10 mM), iberiotoxin (0.1ՌM),... -
Expression of Ca2+-activated K+ Channels and Their Role in Proliferation of Rat Cardiac Fibroblasts
SeyongChoi, WooseokLee, JihyunYun, JeongseokSeo, InjaLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.2 3 51-58 (8 pages)
small conductance KCa (SK1∼4 subunits) channels. Thirdly, we recorded BK, IK, and SK channels by whole cell mode patch clamp technique using their specific blockers. Finally, we performed cell proliferation assay to evaluate the effects of the channels on cell proliferation, and found that the inhibition of IK channel increased the cell proliferation. These results showed the existence of BK, IK, and SK channels in rat ventricular fibroblasts and involvement of IK channel in cell proliferation. -
Differential Functional Expression of Clotrimazole-sensitive Ca2+-activated K+ Current in Bal-17 and WEHI-231 Murine B Lymphocytes
HaifengZheng, JaeHongKo, JooHyunNam, YungEEarm, SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2006, Vol.10 No.1 4 19-24 (6 pages)
expression is upregulated by various immunological stimuli. In this study, the activity of SK4 was compared between Bal-17 and WEHI-231 cell lines which represent mature and immature stages of murine B lymphocytes, respectively. The whole-cell patch clamp with high-Ca2 (0.8μM) KCl pipette solution revealed a voltage-independent K current that was blocked by clotrimazole (1 mM), an SK4 blocker. The expression of mRNAs for SK4 was confirmed in both Bal-17 and WEHI-231 cells. The... -
Two Types of Voltage-activated Calcium Currents in Goldfish Horizontal Cells
Sun-SookPaik, Sun-HoBai, Chang-SubJung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.5 3 269-274 (6 pages)
using a patch-clamping configuration: a transient type current and a sustained type current. The cell was held at 40 mV, and the prepulse step of 90 mV was applied before command pulse between 65 and 55 mV. The transient Ca2 current was activated by depolarization to around 50 mV from a prepulse voltage of 90 mV lasting at least 400 ms and reached a maximal value near 25 mV. On the other hand, the sustained Ca2 current was induced by... -
The Role of Mitochondrial ATP-sensitive Potassium Channel on Intestinal Pacemaking Activity
ByungJooKim, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.4 4 209-214 (6 pages)
slow waves in the gastrointestinal (GI) tract. In the present study, we investigated the effect of mitochondrial ATP-sensitive potassium (mitoKATP) channel on pacemaking activity in cultured ICCs from murine small intestine by using whole-cell patch clamp techniques. Under current clamp mode, at 10μM glibenclamide, there was no change in pacemaking activity of ICCs. At 30μM glibenclamide, an inhibitor of the ATP sensitive K channels, we could find two examples. If pacemaking activity of... -
Protein Kinase C Activates ATP-sensitive Potassium Channels in Rabbit Ventricular Myocytes
NariKim, JaeBoumYoum, HyunJoo, HyungkyuKim, EuiyongKim, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.4 1 187-194 (8 pages)
C (PKC) modulated the activity of KATP channels by recording KATP channel currents in rabbit ventricular myocytes using patch-clamp technique and found that phorbol 12,13-didecanoate (PDD) enhanced pinacidil-induced KATP channel activity in the cell-attached configuration; and this effect was prevented by bisindolylmaleimide (BIM). KATP channel activity was not increased by 4α-PDD. In excised inside- out patches, PKC stimulated KATP channels in the presence of 1 mM ATP, and this effect was... -
Higher Expression of TRPM7 Channels in Murine Mature B Lymphocytes than Immature Cells
JinKyoungKim, JaeHongKo, JooHyunNam, Ji-EunWoo, KyeongMinMin, YungEEarm, SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.2 1 69-76 (8 pages)
TRPM7, a cation channel protein permeable to various metal ions such as Mg2, is ubiquitously expressed in variety of cells including lymphocytes. The activity of TRPM7 is tightly regulated by intracellular Mg2, thus named Mg2-inhibited cation (MIC) current, and its expression is known to be critical for the viability and proliferation of B lymphocytes. In this study, the level of MIC current was compared between immature (WEHI-231) and mature (Bal-17) B lymphocytes. In both... -
Ca2+-activated K+ Currents of Pancreatic Duct Cells in Guinea-pig
Han-WookLee, JingchaoLi, Na-YounKoo, ZhengGenPiao, SungMinHwang, Jae-WoongHan, Han-SaemChoi, Jong-HeunLee, JoongSooKim, KyungpyoPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.6 7 335-338 (4 pages)
a whole-cell patch clamp technique. Both Cl and K conductance were found with KCl rich pipette solutions. When the bath solution was changed to low Cl, reversal potentials shifted to the negative side, 75⁑4 mV, suggesting that this current is dominantly selective to K. We then characterized this outward rectifying K current and examined its Ca2 dependency. The K currents were activated by intracellular Ca2. 100 nM or 500 nM... -
Vibrio vulnificus Cytolysin Forms Anion-selective Pores on the CPAE Cells, a Pulmonary Endothelial Cell Line
Bok-HeeChoi, Byung-HyunPark, Yong-GeunKwak 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.5 4 259-264 (6 pages)
of pulmonary endothelial cell, using inside-out patch clamp techniques. In symmetrical NaCl concentration (140 mM), intracellular or extracellular application of cytolysin formed ion-permeable pores with a single channel conductance of 37.5⁑4.0 pS. The pore currents were consistently maintained after washout of cytolysin. Replacement of Na in bath solution with monovalent ions (K, Cs or TEA) or with divalent ions (Mg2, Ca2) did not affect the pore... -
Characterization of Acetylcholine-induced Currents in Male Rat Pelvic Ganglion Neurons
Joong-HyunPark, Kyu-SangPark, Seung-KyuCha, Keon-IlLee, Min-JungKim, Jong-YeonPark, InDeokKong, Joong-WooLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.4 8 219-226 (8 pages)
acetylcholine receptors (nAChRs) were studied in sympathetic and parasympathetic major pelvic ganglion (MPG) neurons. MPG neurons isolated from male rat were enzymatically dissociated, and ionic currents were recorded by using the whole cell variant patch clamp technique. Total RNA from MPG neuron was prepared, and RT-PCR analysis was performed with specific primers for subunits of nAChRs. ACh dose-dependently elicited fast inward currents in both sympathetic and parasympathetic MPG neurons (EC5... -
Presynaptic Mechanism Underlying Regulation of Transmitter Release by G Protein Coupled Receptors
TomoyukiTakahashi, YoshinaoKajikawa, MasahiroKimura, NaotoSaitoh, TetsuhiroTsujimoto 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.2 1 69-76 (8 pages)
A variety of G protein coupled receptors (GPCRs) are expressed in the presynaptic terminals of central and peripheral synapses and play regulatory roles in transmitter release. The patch-clamp whole-cell recording technique, applied to the calyx of Held presynaptic terminal in brainstem slices of rodents, has made it possible to directly examine intracellular mechanisms underlying the GPCR-mediated presynaptic inhibition. At the calyx of Held, bath-application of agonists for GPCRs such as GABAB... -
Reduction of Muscarinic K+ Channel Activity by Transferrin in Ischemic Rat Atrial Myocytes
KyeongTaePark, DawonKang, JaeheeHan, Jae-YongPark, Chang-GiHur, Seong-GeunHong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.6 7 333-339 (7 pages)
It has been demonstrated that an unidentified cytosolic factor(s) reduces KACh channel function. Therefore, this study attempted to elucidate the cytosolic factor. Fresh cytosol isolated from normal heart (FC) depressed the KACh channel activity, but cytosol isolated from the ischemic hearts (IC) did not modulate the channel function. Electrophorectic analysis revealed that a protein of ∼80 kDa was markedly reduced or even lost in IC. By using peptide sequencing analysis and Western blot, this... -
The Substates with Mutants That Negatively Charged Aspartate in Position 172 Was Replaced with Positive Charge in Murine Inward Rectifier Potassium Channel (Murine Kir2.1)
ISo, I.Ashmole, P.R.Stanfield 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.5 4 267-273 (7 pages)
at pHi 5.5. The larger the size of the side chain or pKa with mutants (D172H, D172K and D172R), the more frequent the transitions between the fully open state and substate within an opening. The conductance of the substate also depended upon the pKa or the size of the side chain. The relative occupancy at substate SLC with monomer D172K (0.50) was less than that in K-H dimer (0.83). However, the relative occupancy at substate with D172R (0.79) was similar to that with R-N dimer (0.82). In the... -
Inhibitory and Excitatory Postsynaptic Currents of Medial Vestibular Nucleus Neurons of Rats
SangWooChun, JeongHeeChoi, ByungRimPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.2 2 59-63 (5 pages)
MVN neurons were studied using whole cell patch clamp recording in slices prepared from 13- to 17-day-old rats. The spontaneous inhibitory postsynaptic currents (sIPSCs) were significantly reduced by the GABAA antagonist bicuculline (20μM), but were not affected by the glycine antagonist strychnine (1μM). The frequency, amplitude, and decay time constant of sIPSCs were 4.3⁑0.9 Hz, 18.1⁑2.0 pA, and 8.9⁑0.4 ms, respectively. Spontaneous excitatory postsynaptic currents (sEPSCs)... -
Modulation of Inwardly Rectifying K+ Channel by Intracellular and Extracellular pH in Bovine Aortic Endothelial Cells
Kyu-SangPark, InDeokKong, JoongWooLee, HyewhonRhim, YoungChulKim, InsukSo, KiWhanKim, 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.5 4 255-260 (6 pages)
the bovine aortic endothelial cells (BAECs) were examined using whole-cell patch-clamp technique. The IRK current, efficiently blocked by Ba2 (200μM), is the most prominent membrane current in BAECs, which mainly determines the resting membrane potential. The expression of Kir2.1 was observed in BAECs using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Intracellular alkalinization, elicited by the extracellular substitution of NaCl with NH4Cl (30 mM), significantly...
