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전체 선택해제
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본문검색 결과만 보기
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Use of hydrophilic extra-viral domain of canine distemper virus H protein for enzyme-linked immunosorbent assay development
Ki-hyun Cho, Jeongmi Kim, Hyun-ah Yoo, Dae-hee Kim, Seung-yong Park, Chang-seon Song, In-soo Choi, Joong-bok Lee, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 6 503-509 (7 pages)
would assist in the effective vaccination of dogs. To develop an enzyme-linked immunosorbent assay (ELISA) specific for CDV, we expressed hydrophilic extra-viral domain (HEVD) protein of the A75/17-CDV H gene in a pET 28a plasmid-based Escherichia (E.) coli vector system. Expression was confirmed by dot and Western blotting. We proposed that detection of E. coli-expressed H protein might be conformation- dependent because intensities of the reactions observed with these two methods varied. The H... -
Role of Nucleotide-binding and Oligomerization Domain 2 Protein (NOD2) in the Development of Atherosclerosis
Ha-JeongKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.6 1 479-484 (6 pages)
NOD2 (nucleotide-binding and oligomerization domain 2) was initially reported as a susceptibility gene for Crohn’s disease, with several studies focused on elucidating its molecular mechanism in the progression of Crohn’s disease. We now know that NOD2 is an intracellular bacterial sensing receptor, and that MDP-mediated NOD2 activation drives inflammatory signaling. Various mutations in NOD2 have been reported, with NOD2 loss of function being associated with the development of Crohn’s... -
A LysM Domain-Containing Protein LtLysM1 Is Important for Vegetative Growth and Pathogenesis in Woody Plant Pathogen Lasiodiplodia theobromae
Dulanjalee Lakmali Harishchandra, Wei Zhang, Xinghong Li, Kandawatte Wedaralalage Thilini Chethana, Kevin David Hyde, Siraprapa Brooks, Jiye Yan, Junbo Peng 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 4호 3 323-334 (12 pages)
a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence analyses revealed that LtLysM1 was a putative glycoprotein with 10 predicted N-glyco- sylation sites and one LysM domain. Pathogenicity tests showed that overexpressed transformants of LtLysM1 displayed increased virulence on grapevine shoots in comparison with that of... -
Generation of Catalytic Protein Particles in Escherichia coli Cells Using the Cellulose-binding Domain from Cellulomonas fimi as a Fusion Partner
Choi. Su-Lim, Lee. Sang-Jun, Ha. Jae-Seok, Song. Jae-Jun, Rhee. Young-Ha, Lee. Seung-Goo 한국생물공학회 Biotechnology and bioprocess engineering 7 Pages
한국생물공학회 Biotechnology and bioprocess engineering 2011, Vol.16 No.6 1173-1179 (7 pages)
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Parkin Interacts with the PDZ Domain of Multi-PDZ Domain Protein MUPP1
장원희, 정영주, 최선희, 이원희, 김무성, 김상진, 엄상화, 문일수, 석대현, Jang. Won Hee, Jeong. Young Joo, Choi. Sun Hee, Lee. Won Hee, Kim. Mooseong, Kim. Sang-Jin, Urm. S 한국생명과학회 생명과학회지 7 Pages
한국생명과학회 생명과학회지 2014, Vol.24 No.8 820-826 (7 pages)
domain을 가지는 단백질로서 여러 구조 단백질 및 신호전달 단백질과 상호 결합하지만, MUPP1이 어떻게 안정화되며, 어떻게 신호전달과정에 관여하는지에 대해 아직 명확히 밝혀지지 않았다. 본 연구에서 MUPP1의 PDZ 도메인과 상호 작용하는 단백질을 규명하기 위하여 효모 two-hybrid 방법을 이용하였고, Parkin이 MUPP1과 결합하는 것을 확인하였다. Parkin은 E3 ubiquitin ligase로서, Parkin 유전자의 기능상실 돌연변이는 autosomal recessive juvenile parkinsonism을 일으키는 것으로 알려져 있다. Parkin은 MUPP1의 12번째 PDZ... -
Cadms/SynCAMs/Necls/TSLCs Interact with Multi-PDZ Domain Protein MUPP1
장원희, 정영주, 최선희, 김상진, 엄상화, 문일수, 석대현, Jang. Won Hee, Jeong. Young Joo, Choi. Sun Hee, Kim. Sang-Jin, Urm. Sang-Hwa, Moon. Il Soo, Seog. Dae-Hyun 한국생명과학회 생명과학회지 8 Pages
한국생명과학회 생명과학회지 2014, Vol.24 No.12 1276-1283 (8 pages)
유지하는 세포 사이 연접복합체는 multi-PDZ domain protein 1 (MUPP1)을 포함하여 50종류 이상의 단백질로 이루어져 있다. MUPP1은 13개의 PDZ 도메인을 가지는 단백질로서 막경유(transmembrane) 단백질과 세포골격단백질이나 신호단백질 사이에서 scaffold로 작용한다고 알려져 있지만, MUPP1이 어떻게 세포막인접 단백질들을 연결하고 구조 안정화에 기여하는지에 대해 아직 명확히 밝혀지지 않았다. 본 연구에서 MUPP1의 PDZ 도메인과 상호 작용하는 단백질을 규명하기 위하여 효모 two-hybrid 방법을 이용, cell adhesion molecule... -
Cloning, Purification, and Structural Characterization by 1D 1H-NMR of the PDZ domain of the Shank3 protein
성미숙, Sung. Mee-Sook 한국생명과학회 생명과학회지 5 Pages
한국생명과학회 생명과학회지 2007, Vol.17 No.3 345-349 (5 pages)
PDZ 도메인을 통하여 여러 단백질과 상호작용하며 신경전달 기전에 관여하는 단백질로 Shank1, Shank2, Shank3, PDS-95, AF있다. 본 연구는 Shank3 PDZ 도메인의 구조를 밝히기 위한 첫 단계로서 Shank3 단백질의 PDZ 도메인을 동정하였고, E. coli에서 발현하여 생성된 단백질을 정제한 후 1차 NMR 구조분석을 시도하였다. 그 결과에 의하면 정제된 Shank3 PDZ 단백질은 순도가 높고 안정적인 접힘(folding)구조를 제시하고있다. 현재 완전한 NMR 구조분석을 위해 좀더 많은 양의 정제된 Shank3 PDZ 단백질을 얻고자 연구하고 있다. -
Extracellular Domain of V-Set and Immunoglobulin Domain Containing 1 (VSIG1) Interacts with Sertoli Cell Membrane Protein, while Its PDZ-Binding Motif Forms a Complex with ZO-1
Kim. Ek-Yune, Lee. Young-Jeon, Kim. Ji-Su, Song. Bong-Seok, Kim. Sun-Uk, Huh. Jae-Won, Lee. Sang-Rae, Kim. Sang-Hyun, Hong. Yong-Geun, Ch 한국분자세포생물학회 Molecules and cells 6 Pages
한국분자세포생물학회 Molecules and cells 2010, Vol.30 No.5 443-448 (6 pages)
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N-Terminal pI Determines the Solubility of a Recombinant Protein Lacking an Internal Transmembrane-like Domain in E. coli
Lee. Sang-Jun, Han. Yun-Hee, Kim. Young-Ok, Nam. Bo-Hye, Kong. Hee-Jeong, Kim. Kyung-Kil 한국분자세포생물학회 Molecules and cells 9 Pages
한국분자세포생물학회 Molecules and cells 2010, Vol.30 No.2 127-135 (9 pages)
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Characteristics of HIV-Tat Protein Transduction Domain
Yoon. Jong-Sub, Jung. Yong-Tae, Hong. Seong-Karp, Kim. Sun-Hwa, Shin. Min-Chul, Lee. Dong-Gun, Shin. Wan-Shik, Min. Woo-Sung, Paik. Soon 한국미생물학회 The journal of microbiology 8 Pages
한국미생물학회 The journal of microbiology 2004, Vol.42 No.4 328-335 (8 pages)
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Surface Display of Organophosphorus Hydrolase on E. coli Using N-Terminal Domain of Ice Nucleation Protein InaV
Khodi. Samaneh, Latifi. Ali Mohammad, Saadati. Mojtaba, Mirzaei. Morteza, Aghamollaei. Hossein 한국미생물생명공학회 Journal of microbiology and biotechnology 5 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2012, Vol.22 No.2 234-238 (5 pages)
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Production of a Fusion Protein Containing the Antigenic Domain 1 of Human Cytomegalovirus Glycoprotein B
Sousa. Fani, Ferreira. Susana, Queiroz. Joao, Domingues. Fernanda 한국미생물생명공학회 Journal of microbiology and biotechnology 6 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2006, Vol.16 No.7 1026-1031 (6 pages)
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Generation and Characterization of Cell-Permeable Greem Fluorescent Protein Mediated by the Basic Domain of Human Immunodeficiency Virus Type 1 Tat
Park. Jin-Seu, Kim. Kyeong-Ae, Ryu. Ji-Yoon, Choi. Eui-Yul, Lee. Kil-Soo, Choi. Soo-Young 한국미생물생명공학회 Journal of microbiology and biotechnology 8 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2000, Vol.10 No.6 797-804 (8 pages)
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Neural Tissue-Specific Epidermal Growth Factor (EGF)-like Domain Containing Protein, NELL2, Plays on Important Role in the Control Regulation of Puberty Onset in the Female Rat Hypothalamus
Ha. Chang-Man, Kang. Hae-Mook, Lee. Byung-Ju 한국동물학회 Korean journal of biological sciences 7 Pages
한국동물학회 Korean journal of biological sciences 2000, Vol.4 No.4 367-373 (7 pages)
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Lack of Mutations in Protein Tyrosine Kinase Domain Coding Exons 19 and 21 of the EGFR Gene in Oral Squamous Cell Carcinomas
Mehta. Dhaval Tushar, Annamalai. Thangavelu, Ramanathan. Arvind 아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 5 Pages
아시아태평양암예방학회 Asian Pacific journal of cancer prevention : APJCP 2014, Vol.15 No.11 4623-4627 (5 pages)
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Metagenome Analysis of Protein Domain Collocation within Cellulase Genes of Goat Rumen Microbes
Lim. SooYeon, Seo. Jaehyun, Choi. Hyunbong, Yoon. Duhak, Nam. Jungrye, Kim. Heebal, Cho. Seoae, Chang. Jongsoo 아세아태평양축산학회 Asian-Australasian journal of animal sciences 8 Pages
아세아태평양축산학회 Asian-Australasian journal of animal sciences 2013, Vol.26 No.8 1144-1151 (8 pages)
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Identification of Transmembrane Domain of a Membrane Associated Protein NS5 of Dendrolimus punctatus Cytoplasmic Polyhedrosis Virus
Chen. Wuguo, Zhang. Jiamin, Dong. Changjin, Yang. Bo, Li. Yanqiu, Liu. Chuanfeng, Hu. Yuanyang 생화학분자생물학회 Journal of biochemistry and molecular biology 6 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 2006, Vol.39 No.4 412-417 (6 pages)
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Peptide Domain Involved in the Interaction between Membrane Protein and Nucleocapsid Protein of SARS-associated Coronavirus
Fang. Xiaonan, Ye. Linbai, Timani. Khalid Amine, Li. Shanshan, Zen. Yingchun, Zhao. Meng, Zheng. Hong, Wu. Zhenghui 생화학분자생물학회 Journal of biochemistry and molecular biology 5 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 2005, Vol.38 No.4 381-385 (5 pages)
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Purification and Spectroscopic Characterization of the Human Protein Tyrosine Kinase-6 SH3 Domain
Koo. Bon-Kyung, Kim. Min-Hyung, Lee. Seung-Taek, Lee. Weon-Tae 생화학분자생물학회 Journal of biochemistry and molecular biology 5 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 2002, Vol.35 No.3 343-347 (5 pages)
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Analysis of the Stoichiometry and the Domain for Interaction of Simian Virus 40 Small-t Antigen with Protein Phosphatase 2A
Yang. Sung-Il, Mumby. Marc C. 생화학분자생물학회 Journal of biochemistry and molecular biology 5 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 1995, Vol.28 No.4 331-335 (5 pages)
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The C-terminal domain of PLD2 participates in degradation of protein kinase CKII β subunit in human colorectal carcinoma cells
Lee. Young-Hoon, Uhm. Jong-Su, Yoon. Soo-Hyun, Kang. Ji-Young, Kim. Eun-Kyung, Kang. Beom-Sik, Min. Do-Sik, Bae. Young-Seuk 생화학분자생물학회 BMB reports 6 Pages
생화학분자생물학회 BMB reports 2011, Vol.44 No.9 572-577 (6 pages)
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Spectrofluorometric Properties of N-Terminal Domain of Lumazine Protein from Photobacterium leiognathi
Kang. Kyoung-Suk, Kim. So-Young, Lee. Jung-Hwan, Nam. Ki-Seok, Lee. Eui Ho, Lee. Chan Yong 대한화학회 Bulletin of the Korean Chemical Society 6 Pages
대한화학회 Bulletin of the Korean Chemical Society 2013, Vol.34 No.6 1673-1678 (6 pages)
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Purification and Characterization of the Amino-Terminal Domain of Lumazine Protein from Photobacterium leiognathi
Kim. So-Young, Kim. Ryu-Ryun, Choi. Ji-Sun, Kim. Young-Doo, Lee. Chan-Yong 대한화학회 Bulletin of the Korean Chemical Society 4 Pages
대한화학회 Bulletin of the Korean Chemical Society 2010, Vol.31 No.4 1017-1020 (4 pages)
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Solution Structure and Backbone Dynamics of the Biotinylation Domain of Helicobacter pylori Biotin-carboxyl Carrier Protein
Jung. Jin-Won, Lee. Chul-Jin, Jeon. Young-Ho, Cheong. Chae-Joon, Lee. Weon-Tae 대한화학회 Bulletin of the Korean Chemical Society 5 Pages
대한화학회 Bulletin of the Korean Chemical Society 2008, Vol.29 No.2 347-351 (5 pages)
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Identification of a Protein Interacting with Human Nebulin SH3 Domain by Yeast Two-hybrid Screening
Lee. Min-A, Kim. Ji-Hee, Min. Byung-In, Park. Soo-Ho, Ko. Han-Suk, Kim. Chong-Rak 대한의생명과학회 The Journal of biomedical laboratory sciences 6 Pages
대한의생명과학회 The Journal of biomedical laboratory sciences 2001, Vol.7 No.2 59-64 (6 pages)
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위암의 Fas-associated Death Domain Protein 단백질의 발현
이석형, 이종우, 박원상, 이정용, 유남진, Lee. Sug-Hyung, Lee. Jong-Woo, Park. Won-Sang, Lee. Jung-Young, Yoo. Nam-Jin 대한위암학회 대한위암학회지 4 Pages
대한위암학회 대한위암학회지 2003, Vol.3 No.2 80-83 (4 pages)
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Mouse Dual Ig Domain Containing Cell Adhesion Molecule Protein Expression and Purification Using the Baculovirus Expression Vector System
Park. Seung-Won, Choi. Ji-Hyun, Goo. Tae-Won, Kim. Seong-Ryul, Lee. Gwang-Gill, Kang. Seok-Woo 대한미생물학회 Journal of bacteriology and virology : JBV 8 Pages
대한미생물학회 Journal of bacteriology and virology : JBV 2010, Vol.40 No.3 123-130 (8 pages)
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Recombinant TAT-CD137 Ligand Cytoplasmic Domain Fusion Protein Induces the Production of IL-6 and TNF-${alpha}$ in Peritoneal Macrophages
Kim. Jung-D., Lee. Eun-A., Quang. Nguyen N., Cho. Hong-R., Kwon. Byung-Suk 대한면역학회 Immune network : official journal of the Korean association of immunobiologists 7 Pages
대한면역학회 Immune network : official journal of the Korean association of immunobiologists 2011, Vol.11 No.4 216-222 (7 pages)
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A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein
Song. Kyoung Ju, Yang. Zhaoshou, Chong. Chom-Kyu, Kim. Jin-Soo, Lee. Kyung Chan, Kim. Tong-Soo, Nam. Ho-Woo 대한기생충학회 The Korean journal of parasitology 8 Pages
대한기생충학회 The Korean journal of parasitology 2013, Vol.51 No.5 503-510 (8 pages)
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Transgenic Tobacco Expressing a Ring Domain-Containing Protein of Capsicum annuum Confers Improved Cold Tolerance
Kim. Kyung-A, Hwang. Eul-Won, Park. Soo-Chul, Jeong. Mi-Jeong, Byun. Myung-Ok, Kwon. Hawk-Bin 한국식물학회 Journal of plant biology 6 Pages
한국식물학회 Journal of plant biology 2007, Vol.50 No.1 44-49 (6 pages)
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Coiled-Coil Domain-Containing Protein 98 (CCDC98) Regulates Cyclin B1 Expression by Affecting WTAP Protein Stability
오윤정, 이은희, 이일규, 김경수, 김홍태, Oh. Yun-Jung, Lee. Eun-Hee, Lee. Il-Kyu, Kim. Kyung-Soo, Kim. Hong-Tae 한국생명과학회 생명과학회지 9 Pages
한국생명과학회 생명과학회지 2011, Vol.21 No.8 1067-1075 (9 pages)
cycle checkpoint에 중요한 역할을 한다고 알려져 있다. 하지만 많은 연구에도 불구하고 CCDC98 단백질의 기능에 대해서 알려진 바가 거의 없다. 본 연구는 CCDC98 단백질의 기능을 밝히고자 tandem affinity purification 방법을 수행하였다. 그 결과 Wilms tumor 1-associating protein (WTAP)을 CCDC98의 결합 단백질로 분리 동정하였다. 이들 단백질의 결합을 in vivo and in vitro binding assays를 통하여 확인하였다. 또한, CCDC98 단백질이 cyclin B1의 발현을 억제함을 확인하였는데, 이는 WTAP 단백질의 발현을 조절함으로써... -
Structural Characterization of the J-domain of Tid1, a Mitochondrial Hsp40/DnaJ Protein
Sim. Dae-Won, Jo. Ku-Sung, Ryu. Kyoung-Seok, Kim. Eun-Hee, Won. Hyung-Sik 한국자기공명학회 Journal of the Korean magnetic resonance society 12 Pages
한국자기공명학회 Journal of the Korean magnetic resonance society 2012, Vol.16 No.1 22-33 (12 pages)
