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Characterization of Trichoderma spp. Associated with Green Mold of Oyster Mushroom by PCR-RFLP and Sequence Analysis of ITS Regions of rDNA
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2005, 21권 3호 5 229-236 (8 pages)
Molecular profiles of PCR-RFLP and sequence analysis of internal transcribed spacer (ITS) regions of rDNA were compared between morphologically distinguishable species of Trichoderma isolated from substrates of oyster mushroom in Korea, T. atroviride, T. citrinoviride,T. harzianum, T. longibrachiatum, T. virens, and two unidentified species, Trichoderma sp. 1 and 2. PCRRFLP analysis divided the Trichoderma spp. into six RFLP groups, A, B, C, D, E, and F. The RFLP groups were generally agreed... -
DNA Analysis of mtDNA COI Gene in the Sharp-toothed Eel (Muraenesox cinereus Forskal) from Yeosu, Jinhae, Jeju, Goseoung, Jangheung and Haenam Populations in Korea Using PCR-aided RFLP
Oh. Taeg-Yun, Jeong. Sun-Beom, Cho. Eun-Seob 한국환경과학회 한국환경과학회지 4 Pages
한국환경과학회 한국환경과학회지 2011, Vol.20 No.4 551-554 (4 pages)
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Differentiation of Lactic Acid Bacteria Based on RFLP Analysis of the tuf Gene
Park. Seok-Hwee, Jung. Jong-Hyun, Seo. Dong-Ho, Lee. Hye-Lim, Kim. Gye-Won, Park. Sun-Young, Shin. Woo-Chang, Hong. Sung-Youl, Park. Che 한국식품과학회 Food science and biotechnology 5 Pages
한국식품과학회 Food science and biotechnology 2012, Vol.21 No.3 911-915 (5 pages)
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Cultural characteristics and phylogenetic relationships based on RFLP analysis in Pleurotus species
Choi. Sun-Gyu, Jang. Kab-Yeul, Kim. Gyu-Hyun, Kong. Won-Sik, Jo. Jae-Sun, Kim. Hae-Yeong, Yoo. Young-Bok 한국버섯학회 Journal of mushrooms 9 Pages
한국버섯학회 Journal of mushrooms 2014, Vol.12 No.3 145-153 (9 pages)
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RFLP Analysis of cag7 Gene of Helicobacter pylori
Kang. Hyung-Lyun, Park. Jeong-Uck, Choe. Mi-Young, Kim. Kyung-Mi, Kim. Do-Su, Kwan. Young-Chul, Park. Seung-Gyu, Hwang. Hyang-Ran, Song. 대한미생물학회 Journal of bacteriology and virology : JBV 10 Pages
대한미생물학회 Journal of bacteriology and virology : JBV 2004, Vol.34 No.3 171-180 (10 pages)
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Use of Terminal Restriction Length Polymorphism (T-RFLP) Analysis to Evaluate Uncultivable Microbial Community Structure of Soil
Chauhan. Puneet Singh, Shagol. Charlotte C., Yim. Woo-Jong, Tipayno. Sherlyn C., Kim. Chang-Gi, Sa. Tong-Min 한국토양비료학회 韓國土壤肥料學會誌 19 Pages
한국토양비료학회 韓國土壤肥料學會誌 2011, Vol.44 No.1 127-145 (19 pages)
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Genome Organization in Coffee as Revealed by EST PCR-RFLP, SNPs and SSR Analysis
Mishra. Manoj Kumar, Tornincasa. Patrizia, Nardi. Barbara De, Asquini. Elisa, Dreos. Rene, Terra. Lorenzo Del, Rathinavelu. Rajkumar, R 한국작물학회 Journal of crop science and biotechnology 13 Pages
한국작물학회 Journal of crop science and biotechnology 2011, Vol.14 No.1 25-37 (13 pages)
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Application of Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis to Monitor Effect of Biocontrol Agents on Rhizosphere Microbial Community of Hot Pepper (Capsicum annuum L.)
Kim. Young-Tae, Cho. Myoung-Ho, Jeong. Je-Yong, Lee. Hyang-Burm, Kim. Seung-Bum 한국미생물학회 The journal of microbiology 7 Pages
한국미생물학회 The journal of microbiology 2010, Vol.48 No.5 566-572 (7 pages)
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A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis
Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 9 233-239 (7 pages)
agreement analysis for which differences in the number of cycles to positive (CP) were compared by Student’s t-test for paired samples and regression analysis. Twelve out of 104 fecal pools cultured were positive. The final PCR results for 11 samples analyzed with the QIAamp and UACH methods or ones examined with the QIAamp and CTAB methods were in agreement. Complete (100%) agreement was observed between data from the CTAB and UACH methods. CP values for the UACH and CTAB techniques were not... -
Genetic IS901 RFLP diversity among Mycobacterium avium subsp. avium isolates from four pheasant flocks
Monika Moravkova, Jiri Lamka, Michal Slany, Ivo Pavlik* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 15 99-102 (4 pages)
IS901 RFLP analysis of 36 Mycobacterium avium subsp. avium (MAA) isolates from 15 pheasants (Phasianus colchicus) and two goshawks (Accipiter gentilis) from four pheasant farms was performed. Using this method, six different IS901 RFLP types (E, F, G, M, Q, and V) were identified. The distribution of IS901 RFLP profiles was tightly linked to individual flocks. Matching IS901 RFLP profiles observed in the present study indicate MAA transmission between pheasants and goshawks in the same locality.... -
Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do, Korea
Bo-Young Jeon, Sungmo Je, Jinhee Park, Yeun Kim, Eun-Gae Lee, Hyeyoung Lee, Sangkyo Seo, Sang-Nae Cho* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 4 145-154 (10 pages)
Bovine tuberculosis (TB) is a major zoonosis that's caused by Mycobacterium bovis (M. bovis). Being able to detect M. bovis is important to control bovine TB. We applied a molecular technique, the variable number tandem repeat (VNTR) typing method, to identify and distinguish the M. bovis isolates from Gyeonggi-do, Korea. From 2003 to 2004, 59 M. bovis clinical strains were isolated from dairy cattle in Gyeonggi-do, Korea, and these cattle had tuberculosis-like lesions. Twenty-four published... -
Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea
Ji-Hyun Jang, Haan-Woo Sung, Chang-Seon Song, Hyuk-Moo Kwon* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 13 401-408 (8 pages)
(RFLP) analysis. These Korean IBV isolates were classified into three groups according to their RFLP patterns obtained using the restriction enzyme HaeIII. Half of the twelve isolates were similar to the KM91 RFLP pattern, which is a common pattern in Korea. Three more isolates were related to the Arkansas strain pattern, but with some unique variations. The other three viruses showed variant RFLP patterns. For a comparison with the published sequences for non-Korean IBV strains, amplified PCR... -
Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
J. P. N. Singh, Rishendra Verma, P. Chaudhuri 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 15 181-187 (7 pages)
of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected to RAPD-PCR using 7 random decamer primers. All 7 primers were found to be differentiated and produced specific RAPD profiles. The polymorphic amplicons served as RAPD markers for M. tuberculosis. The dendrograms, obtained by different primers, showed the discriminatory ability of the primers. RAPD... -
Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles
Dong Kyun Suh, Jae Chan Song 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 6 37-41 (5 pages)
A total of 22 Salmonella enterica serotype Enteritidis (S. Enteritidis)strains isolated from human and chicken were subjected to DNA fingerprinting by repetitive sequence PCR using ERIC and BOX primers, antibiotic resistance and plasmid patterns.Both ERIC and BOX PCR amplification data revealed a highly genetic homogeneity between isolates from human and chicken except one isolate, which originated from chicken and showed a different DNA band pattern from others.Eleven of 22 S. Enteritidis... -
Molecular fingerprinting of clinical isolates of Mycobacterium bovis and Mycobacterium tuberculosis from India by restriction fragment length polymorphism (RFLP)
Sandeep Kumar Singh, Rishendra Verma, Devendra H. Shah 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 8 331-335 (5 pages)
the characteristic 1.9kb restriction fragment. RFLP analysis with IS1081 invariably showed the presence of 5 copies in all isolates of M.bovis and M.tuberculosis at the same chromosomal location. Similarity of IS6110 RFLP fingerprints of M.tuberculosis strains from animals and human suggested the possibility of dissemination of single M.tuberculosis strain among animals as well as human. It was not possible to discriminate within the isolates of either M.tuberculosis or M.bovis, when IS1081 was... -
Original Article : Characterization of antimicrobial resistance and application of RFLP for epidemiological monitoring of thermophilic Campylobacter spp. isolated from dogs and humans in Korea
Hyun Ho Cho , Sang Hyun Kim , Won Gi Min , Bok Kyung Ku , Jong Hyun Kim , Yong Hwan Kim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2014, 제 54권 제 2호 5 91-99 (9 pages)
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Phylogenetic Analysis of Phaeosphaeria Species Using Mating Type Genes and Distribution of Mating Types in Iran
Fariba Ghaderi, Azadeh Habibi, Bahram Sharifnabi 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 2 78-89 (12 pages)
Phaeosphaeria species are pathogenic on wheat, barley and a wide range of wild grasses. To analyze mating type loci of the Phaeosphaeria species and investigate mating type distribution in Iran, we sequenced mating type loci of 273 Phaeosphaeria isolates including 67 isolates obtained from symptomatic leaves and ears of wheat, barley, and wild grasses from two wheatgrowing region in Iran as well as 206 isolates from our collection from other regions in Iran which were isolated in our previous... -
Analysis of Fungicide Sensitivity and Genetic Diversity among Colletotrichum Species in Sweet Persimmon
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 3 115-122 (8 pages)
to the same fungicide. PCR-restriction fragment length polymorphism (RFLP) analyses were performed to detect internal transcribed spacer regions and the β-tubulin and glutamine synthetase genes of the pathogens examined. Both the glutamine synthetase and β-tubulin genes contained a complex set of polymorphisms. Based on these results, the pathogens isolated from organic cultivation orchards were found to have more diversity than the isolates obtained from the practical management orchards. -
Note : Open Access ; Comparative Analysis of the Korean Population of Magnaporthe oryzae by Multilocus Microsatellite Typing
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 4호 9 435-439 (5 pages)
gene diversity and allele frequency were 0.412 and 6.5, respectively. Comparative analysis of the digitized allele information revealed that the Korean population exhibited a similar level of allele diversity to the integrated diversity of the world populations, suggesting a particularly high diversity of the Korean population. Therefore, these microsatellite markers and the comprehensive collection of field isolates will be useful genetic resources to identify the genetic diversity of M. oryzae... -
Genetic Variation in Fusarium oxysporum f. sp. fragariae Populations Based on RAPD and rDNA RFLP Analyses
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2004, 20권 4호 7 264-270 (7 pages)
region of rDNA were used to identify genetic variation among 22 F. oxysporum f. sp. fragariae isolates. All isolates could be distinguished from each other by RAPD analysis and RFLP of 2.6 kb amplified with primer CNS1 and CNL12 for IGS region of rDNA. Cluster analysis using UPGMA showed eight distinct clusters based on the banding patterns obtained from RAPD and rDNA RFLP. These results indicate that F. oxysporum f. sp. fragariae isolates are genetically distinct from each other. There was a... -
Molecular Detection and Analysis of Sweet potato feathery mottle virus from Root and Leaf Tissues of Cultivated Sweet Potato Plants
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2002, 18권 1호 3 12-17 (6 pages)
fg. The virus was rapidly detected from total nucleic acids of leaves and roots from the virus-infected sweet potato plants as well as from the purified viral RNA by the RTPCR. Twenty-four sweet potato samples were selected and analyzed by RT-PCR and restriction fragment length polymorphism (RFLP). RFLP analysis of the PCR products showed three restriction patterns, which resulted in some point mutations suggesting the existence of quasi-species for the virus in the infected sweet potato plants. -
Authentication of Salted-dried Fish Species Using Polymerase Chain Reaction-Single Strand Conformational Polymorphism and Restriction Analysis of Mitochondrial DNA
Kim. Joo-Shin, Chu. Kin Kan Astley, Kwan. Hoi Shan, Chung. Hau Yin 한국수산과학회 Journal of fisheries science and technology 7 Pages
한국수산과학회 Journal of fisheries science and technology 2008, Vol.11 No.3 133-139 (7 pages)
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RT-PCR과 RFLP법을 이용한 국내 소 로타바이러스 VP4 및 VP7 유전자의 특성 규명
전성진, 장정호, 정정원, 김원용, 강신영, Jeon. Seong-Jin, Chang. Chung-Ho, Chung. Chung-Won, Kim. Won-Yong, Kang. Shien-Young 대한바이러스학회 대한바이러스학회지 10 Pages
대한바이러스학회 대한바이러스학회지 1998, Vol.28 No.2 165-174 (10 pages)
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Restriction endonuclease analysis of mitochondrial DNA of Acanthamoebn sp. YM-4 (Korean isolate)
신호준, 임경일, 전광우, Sin. Ho-Jun, Im. Gyeong-Il, Jeon. Gwang-U 대한기생충학회 The Korean journal of parasitology 8 Pages
대한기생충학회 The Korean journal of parasitology 1997, Vol.35 No.2 119-126 (8 pages)
미토콘드리아 DNA(mtDNA)를 분리하여. 여러 종의 제한효소를 처리함으로써 mtDNA의 단편들을 얻은 다 전체 크기 및 제한효소 절단 단편길이 다형성(RFLP) 분석을 하였다. 5가지의 제한효소 즉 Hce III. Hind III . Cla I. pvu II 빛 Sal I으로 처리된 Acanthamoebn의 mtDNA는 최소 3개의 단편들고부터 많은 것은 15개의 단편들로까지 나뉘어졌다. 단편들을 합산한 mtDNA의 전체 크기는 Acnnthcmoebc sp. YM-4가 평균 46.4 kbp였으며 A. culbertsoni 및 A. potwphcgc는 각각 48.3 kbp 및 48.8 kbp로 관찰되었다 제한효소 단편길이들은 0. 6... -
Population Analysis of Korean and Japanese Toxic Alexandrium catenella Using PCR Targeting the Area Downstream of the Chloroplast PsbA Gene
Kim. Choong-Jae, Kim. Chang-Hoon, Sako. Yoshihiko 한국수산과학회 Journal of fisheries science and technology 6 Pages
한국수산과학회 Journal of fisheries science and technology 2004, Vol.7 No.3 130-135 (6 pages)
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Investigation of Genetic Diversity of Fusarium oxysporum f. sp. fragariae Using PCR-RFLP
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 2호 6 140-147 (8 pages)
sp. fragariae species-specific primer sets. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses were executed using the internal transcribed spacer, intergenic spacer, translation elongation factor1-α, and β-tubulin genes of the pathogens and four restriction enzymes: AluI, HhaI, HinP1I and HpyCH4V. Regarding results, there were diverse patterns in the three gene regions except for the β-tubulin gene region. Correlation analysis of... -
The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Tae Jung Kim, Jae Il Lee 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 11 277-280 (4 pages)
(B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same pattern. These findings are not consistent with a prior... -
Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP
Muhammad Kamran, Nazir Javed, Ihsan Ullah, Shahid Nazir, Shakra Jamil, Muhammad Zafar Iqbal, Huma Abbas, Sajid Aleem Khan, Muhammad Ehetisham ul Haq 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 6 51-62 (12 pages)
A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures (25 ± 2°C and 30 ± 2°C) and concentrations (104 and 105 spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed... -
Molecular Analysis of Botrytis cinerea Causing Ginseng Grey Mold Resistant to Carbendazim and the Mixture of Carbendazin Plus Diethofencarb
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 4호 3 322-327 (6 pages)
for glutamate led the resistance to carbendazim and the mixture at the same position of amino acid. PCR-restriction fragment length polymorphism (PCR-RFLP) analysis using the restriction endonuclease, Tsp45I and BstUI allowed differentiation of the PCR fragment of β-tubulin gene of CarSMixS isolates from that of CarRMixR and CarRMixS isolates. This method will aid in a fast detection of resistance of carbendazim and the mixture of carbendazim plus diethofencarb in B. cinerea in ginseng field. -
Use of Restriction Fragment Length Polymorphism Analysis to Differentiate Fungal Strains in Sunchang Meju
Jung. Jong-Hyun, Seo. Dong-Ho, Bhoo. Sung-Hee, Ha. Suk-Jin, Kim. Jong-Sang, Kim. Jeong-Hwan, Kwon. Dae-Young, Cha. Jae-Ho, Park. Cheon-S 한국식품과학회 Food science and biotechnology 4 Pages
한국식품과학회 Food science and biotechnology 2008, Vol.17 No.4 888-891 (4 pages)
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Analysis of SNPs in Bovine CSRP3, APOBEC2 and Caveolin Gene Family
삼술부이얀, 유성란, 김관석, 윤두학, 박응우, 전진태, 이준헌, Bhuiyan. M.S.A., Yu. S.L., Kim. K.S., Yoon. D., Park. E.W., Jeon. J.T., Lee. J.H. 한국동물자원과학회 한국동물자원과학회지 10 Pages
한국동물자원과학회 한국동물자원과학회지 2007, Vol.49 No.6 719-728 (10 pages)
morphism; SNP)을 8개의 다른 소의 품종에서 확인한 결과 coding region에서 caveolin family 유전자에서 9개의 SNP, CSRP3유전자에서 1개의 SNP 및 APOBEC2 유전자에서 3개의 SNP가 존재함을 확인하였다. 이 coding region의 SNP들은 PCR-RFLP 방법에 의해 재확인하였으며 이들 유전자의 intronic region에서도 9개의 SNP가 존재함을 확인할 수 있었다. 8개의 다른 품종 소에 각 유전자들의 SNP들을 이용하여 유전자 빈도를 확인한 결과 CAV2, CAV3, CSRP3 및 APOBEC2 유전자의 SNP 중에서 5개가 품종간에서 유의적으로 차이가 있음을... -
Analysis of $LH{eta}$ Exon 3 (Gly102Ser) Gene Mutation in Infertile Patients with Endometriosis and Polycystic Ovary Syndrome (PCOS)
김남근, 이유진, 조민순, 남윤성, 정형민, 정기화, 오유경, 고정재, 차광열, Kim. Nam-Keun, Lee. Eu-Gene, Cho. Min-Soon, Nam. Yoon-Sung, Chung. Hyung-Min, Chung. Ki-Wha, Oh. Y 대한생식의학회 대한불임학회지 4 Pages
대한생식의학회 대한불임학회지 2000, Vol.27 No.3 291-294 (4 pages)
환자 그리고, 50명의 출산 경험이 있는 건강한 여성을 대조군으로 사용하였다. 이들을 대상으로 한 돌연변이 탐색은 PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) 방법으로 수행되었다. 결과 : 그 결과 자궁내막증과 다낭성 난포증후군 환자 및 출산 경험이 있는 건강한 여성에서 그 변이형이 나타나지 않았다. 결론: 따라서, 자궁내막증과 다낭성 난포증후군 불임환자의 $LH{eta}$ exon 3 돌연변이형은 중국인 집단에만 존재할 가능성이 높으며, 더 많은 불임환자들을 대상으로 한 연구가 요구된다. -
Four Cases of Taenia saginata Infection with an Analysis of COX1 Gene
Cho. Jaeeun, Jung. Bong-Kwang, Lim. Hyemi, Kim. Min-Jae, Yooyen. Thanapon, Lee. Dongmin, Eom. Keeseon S., Shin. Eun-Hee, Chai. Jong-Yil 대한기생충학회 The Korean journal of parasitology 5 Pages
대한기생충학회 The Korean journal of parasitology 2014, Vol.52 No.1 79-83 (5 pages)
