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전체 선택해제
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DEVELOPMENT OF A KNOWLEDGE-BASED HYBRID FAILURE DIAGNOSIS SYSTEM FOR URBAN TRANSIT
Kim. H.J., Bae. C.H., Kim. S.H., Lee. H.Y., Park. K.J., Suh. M.W. 한국자동차공학회 International journal of automotive technology 7 Pages
한국자동차공학회 International journal of automotive technology 2009, Vol.10 No.1 123-129 (7 pages)
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Production of transgenic pigs using a pGFAP-CreERT2/EGFP LoxP inducible system for central nervous system disease models
Seon-Ung Hwang, Kiyoung Eun, Junchul David Yoon, Hyunggee Kim, Sang-Hwan Hyun 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 13 434-445 (12 pages)
confirmed by immunofluorescence staining and PCR. Real-time PCR analysis showed that pGFAP promoter-driven Cre fused to the mutated human ligand-binding domain of the estrogen receptor (CreERT2) mRNA was highly expressed in the cerebrum. Semi-nested PCR analysis revealed that CreERT2-mediated recombination was induced in cerebrum and cerebellum but not in skin. Thus, we successfully generated a TG pig with a 4-hydroxytamoxifen (TM)-inducible pGFAP-CreERT2/EGFPLoxP recombination system via SCNT. -
Knowledge, attitudes and practices toward antimicrobial usage: a cross-sectional study of layer and pig farm owners/managers in Chiang Mai, Lamphun, and Chonburi provinces, Thailand, May 2014 to February 2016
Aniroot Nuangmek , Suvichai Rojanasthien , Prapas Patchanee , Terdsak Yano , Panuwat Yamsakul , Suwit Chotinun , Pakpoom 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2018, 제 58권 제 1호 3 17-25 (9 pages)
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A Study of Singapore Music Teacher Training System
Mikyung Rim, Eunsil Park, Hye-Suk Lim 한국음악교육학회 음악교육연구 21 Pages
한국음악교육학회 음악교육연구 2020, 제 49권 제 2호 8 197-217 (21 pages)
The purpose of this study was to explore the characteristics of Singapore's music teacher training process. The research method was to investigate and analyze the information regarding this topic mainly through the web sites of Singapore Ministry of Education, National Institute of Education, Nanyang Technological University and Ats Institutes in Singapore. The results of the study are: 1) Singapore has three tracks of music teacher training courses(diploma, bachelor degree, postgraduate degree... -
Correlation Analysis between Dementia Attitude and Dementia Knowledge According to the Level of Dementia Education of Occupational Therapy Students
Jung Hye-Young, Yoo Chan-Uk 고령자치매작업치료학회 고령자·치매작업치료학회지 8 Pages
고령자치매작업치료학회 고령자·치매작업치료학회지 2021, 제 15권 제 1호 8 75-82 (8 pages)
This is to find out knowledge of dementia and the attitude of to be occupational therapy students who work in the field of dementia, and to find how knowledge of dementia and the attitude are correlated depending on the level of regular dementia education and regular clinical practicum of dementia in college. Methods : The survey was conducted on college students majoring occupational therapy from January to March 2021. Explanation on the study was given to all participants and only the students... -
교실 및 온라인 토론수업을 위한 준비 교수체제 (Preparatory Instructional System) 설계모형 개발
한국교육방법학회 교육방법연구 2017, 제 29권 제 4호 5 677-705 (29 pages)
본 연구는 교실 및 온라인에서 진정한 의미의 토론수업이 이루어지기 위해, 사전에 설계되고 실행되 어야 할 준비 교수체제 모형과 이에 대한 실천적 교수전략을 개발하는데 그 목적을 두고 있다. 이에 대한 연구문제를 해결하기 위해 Schreier(2012)의 내용분석 모형과 더불어 Reigeluth와 Frick(1999)의 교 육방법 선정 기준에 주로 입각하여, 문헌분석을 실시하였다. 이러한 연구 결과, 교실 및 온라인토론 학습의 핵심 실패요인으로 토론에 대한 교사의 비현실적인 기대, 토론규칙의 결여, 교수자의 역할모 범 미흡, 준비가 결여된... -
Investigation of the Reliability of Knowledge Source in CLINAID using Fuzzy Relational Method
노찬숙, Noe. Chan-Sook 한국지능시스템학회 퍼지 및 지능시스템학회 논문지 9 Pages
한국지능시스템학회 퍼지 및 지능시스템학회 논문지 2003, Vol.13 No.2 222-230 (9 pages)
의료 시스템이 개발되면 시스템이 사용하는 knowledge source의 신뢰도가 시스템의 수행능력에 큰 영향을 미치게 되므로, knowledge source의 신뢰도를 검증해야한다. 본 논문은 의료 시스템 CLINAID의 knowledge source의 신뢰성 조사에 대한 연구의 방법과 결과를 발표하였다. 그 방법으로는 CLINAID에 사용된 Cardiovascular body system 데이터에 fuzzy relational method를 적용하여 구조적 분석을 통해 만들어진 인공의 syndrome을 knowledge base에 저장되어있는 의료 전문가의 syndrome과 비교하였다. 7 가지 fuzzy implication... -
Development of Grinding Expert System by Fuzzy Model
김남경, 김건회, 송지복, Kim. Nam-Gyeong, Kim. Geon-Hoe, Song. Ji-Bok 한국정밀공학회 정밀공학 17 Pages
한국정밀공학회 정밀공학 1991, Vol.8 No.3 27-43 (17 pages)
공학적 지식 보다는 숙련자의 경험과 기능에 의존하고 있는 실정이다. 본 연구에서는 이와 같은 국면에 대처한 문제 해결을 위해 Computer가 인간사고에 근접 할 수 있도록 Fuzzy 이론과 Default 이론을 도입하고 전문가의 이론적 지식과 숙련자의 감각적 지식을 적극 수용 하여 연소용 Expert system (최적 가공 조건의 설정 System과 Trouble shooting system)을 개발하였다. 또한 연소 가공 Data의 불확실한 애매성을 효과적으로 이용 할 수 있도록 Fuzzy 가능성이론에 의해 가공 Datad을 회귀 분석하여 실가공 Data base에 축적시켜... -
What Knowledge and Skills Do Chinese Kindergarten Teachers Need in a Time of Reform: Director’s Perspectives
Xin Fan, Berenice Nyland, Chris Nyland 환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 22 Pages
환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 2016, Vol.10 No.3 2 27-48 (22 pages)
a renewed interest in early childhood education. Subsequently, policy makers have raised questions regarding what early childhood teachers should know and the skills they need to acquire to be competent practitioners. The research findings draw on interviews conducted with 24 kindergarten directors from provinces across China. The interview explored opinions about skills and attributes teachers require, level and form of knowledge they need, and how teachers’ capacities might be enhanced. -
The meaning of Skillful Means and the Conventional Truth in Buddhist Education
Kim, Yong-pyo 한국종교교육학회 종교교육학연구 18 Pages
한국종교교육학회 종교교육학연구 2005, 제 21권 7 177-194 (18 pages)
truth as teaching device. And the educational significance of the truth of savti in Buddhist educational system is explored. Buddhist scriptures are comprised of emancipatory words. Of necessity they are spoken to others, their aim being removal of human suffering. Not only does the Buddha arrive at emancipatory experience, he also formulates the experience in everyday language. This language is the very essence of the Bodhisattva praxis in Mahyna Buddhism. The scriptural texts are the Buddha s... -
BEYOND YOUNG PEOPLE’S PRIVACY ONLINE: DATA LITERACY PROJECTS FOR CRITICAL DATA EDUCATION
Luci Pangrazio 경인교육대학교 교육연구원 The Journal of Education 21 Pages
경인교육대학교 교육연구원 The Journal of Education 2020, Vol.3 No.2 2 19-39 (21 pages)
This paper is based on a presentation given at the ‘Rethinking Literacy, Digital Competency and Media Education in the Age of Digital Platforms’ webinar hosted by Gyeongin National University in September 2020. In the paper, I explore the difficulties of implementing ‘critical data education’ programs in schools with children and young people. Reporting on three research projects with over 150 Australian students (aged 8-16 years), I explore the challenges and opportunities that arose. Each... -
Challenges of Effective Teaching of Chemistry in Secondary Schools of Nepal
Indra Raj Upadhyaya 경인교육대학교 교육연구원 The Journal of Education 15 Pages
경인교육대학교 교육연구원 The Journal of Education 2019, Vol.2 No.2 5 55-69 (15 pages)
for the analysis. The challenges for effective teaching of chemistry was found in several areas like teaching methods, classroom size, interest of students, classroom environment, development of chemical concept, gap in the curriculum, evaluation system, professionalism of chemistry teachers, and laboratory work. Study established that teacher related factors and school environment plays important role in the effective teaching of chemistry. Government and other stakeholders in education should... -
Analysis of Operation and Development of Online Learning Systems for Software Education
Han Sun-Gwan 경인교육대학교 교육연구원 The Journal of Education 14 Pages
경인교육대학교 교육연구원 The Journal of Education 2019, Vol.2 No.1 1 1-14 (14 pages)
a result of the operation of the system, users have been steadily increasing since 2016. The average monthly number of users in 2016 was 3,056, and 3,463 in 2017, 4,365 monthly averages in 2018, which is an annual increase. As a result of detailed analysis, users increased in June and November. This shows that elementary and junior high school students have used the system as a pre-vacation activity. To expand the base of Software Education, online learning system by developing in this study is... -
외과학 임상실습 평가방법의 변경이 실습성적에 미치는 영향
김명수(Myoung Soo Kim), 김익용(Ik Yong Kim), 배금석(Keum Suck Bae), 한애리(Airi Han), 김대성(Dae Sung Kim), 유수영(Soo Young Yoo), 강성준(Seong-Joon Kang), 한국의학교육학회 Korean Journal of Medical Education 11 Pages
한국의학교육학회 Korean Journal of Medical Education 2003, 제 15권 제 3호 6 221-231 (11 pages)
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가정의학과 전공의를 위한 웹 기반 포트폴리오 평가시스템의 설계 및 구현
김준수(Jun Su Kim), 호승희(Seung Hee Ho), 이정권(Jungkwon Lee) 한국의학교육학회 Korean Journal of Medical Education 11 Pages
한국의학교육학회 Korean Journal of Medical Education 2006, 제 18권 제 3호 5 259-269 (11 pages)
three (13.0%) reported having no problems. The web-based portfolio assessment system was successfully designed to be easy to implement and user-friendly. Conclusion: The web-based portfolio assessment system is expected to overcome the shortcomings of a paper-based portfolio assessment system and improve the level of satisfaction of medical residents. It is expected that the web-based portfolio assessment system designed in this study will be an additional tool for family medicine residencies. -
Mitochondrial dysfunction and cytochrome ctranslocation induced by resveratrol are dependent on NF-κB activity but not autophagy flux and facilitate TRAIL sensitivity in lung cancer
Rasheduzzaman Mohammad, Sang-Youel Park, Jeong-Min Hong, Hong-hua Yin, Nazim Uddin MD, You-Jin Lee 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 50 226-227 (2 pages)
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Ovary and vaginal epithelium dynamics during the estrous cycle in Dasyprocta prymnolopha Wagler, 1831
Artur N. Carreiro, Joã, o A. R. A. Diniz, Joyce G. Souza, Dé, bora V. F. Araú, jo, Rô, mulo F. F. Dias, Liliane M. S. Aze 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 14 446-451 (6 pages)
The objective of the present study was to monitor and characterize morphological alterations in ovaries of agouti (Dasyprocta prymnolopha), reared in captivity, by using abdominal ultrasonography. All animals underwent daily vaginal cytological examination to identify the current cycle phase. For each phase of the estrous cycle, ultrasound examinations were carried out to identify and describe the morphology of both ovaries. Topographic parameters in an ultrasound window were established to... -
Polymorphism in the serotonin transporter protein gene in Maltese dogs with degenerative mitral valve disease
Chang-Min Lee, Jae-Ik Han, Min-Hee Kang, Seung-Gon Kim, Hee-Myung Park 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 16 129-135 (7 pages)
Degenerative mitral valve disease (DMVD) is the most commonly acquired cardiac disease in dogs. This study evaluated the relationship between genetic variations in the serotonin transporter (SERT) gene of Maltese dogs and DMVD. Genomic DNA was extracted from blood samples collected from 20 client-owned DMVD Maltese dogs and 10 healthy control dogs, and each exon of the SERT gene was amplified via polymerase chain reaction. The resulting genetic sequences were aligned and analyzed for variations... -
Inflammation affects the viability and plasticity of equine mesenchymal stem cells: possible implications in intra-articular treatments
Laura Barrachina, Ana Rosa Remacha, Antonio Romero, Francisco Jos? V?zquez, Jorge Albareda, Marta Prades, Beatriz Ranera, Pilar 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 1호 6 39-49 (11 pages)
Mesenchymal stem cells (MSCs) are gaining relevance for treating equine joint injuries because of their ability to limit inflammation and stimulate regeneration. Because inflammation activates MSC immunoregulatory function, proinflammatory priming could improve MSC efficacy. However, inflammatory molecules present in synovial fluid or added to the culture medium might have deleterious effects on MSCs. Therefore, this study was conducted to investigate the effects of inflammatory synovial fluid... -
Deep anterior lamellar keratoplasty of dog eyes using the big-bubble technique
Soohyun Kim, Ji Yoon Kwak, Manbok Jeong, Kangmoon Seo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 11 347-352 (6 pages)
This study was conducted to establish the feasibility of corneal transplantation using the big-bubble technique (BBT) to perform deep anterior lamellar keratoplasty (DALK) in three dogs. After the cornea was trephined 750 ?m, 4 mL of air was injected, and the blanched stroma was removed to expose Descemet’s membrane (DM). The donor corneal button, which was gently stripped off the DM, was sutured onto the bare DM of the recipient cornea. The dogs received topical antibiotics every 6 h for 7... -
Helicobacter apodemus sp. nov., a new Helicobacter species identified from the gastrointestinal tract of striped field mice in Korea
Woo Jin Jeon, Hee-Jin Dong, Jae Hoon Shin, Il Yong Kim, Hungwui Ho, Seung Hyun Oh, Young Min Yoon, Yang-Kyu Choi, Jun Gyo Suh, K 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 11 475-481 (7 pages)
A novel Helicobacter species was identified from the gastrointestinal tract of the Korean striped field mouse (Apodemus agrarius). Biochemical testing, ultrastructure characterization, and 16S rRNA gene sequence analysis suggested that this bacterium represents a distinct taxon. The bacterium was positive for urease activity, susceptible to cephalothin and nalidixic acid, and weakly positive for oxidase and catalase activity. Electron microscopy revealed that the bacterium has spirally curved... -
β-irradiation (Ho patch)-induced skin injury in mini-pigs: effects on NF-κB and COX-2 expression in the skin
Joong-Sun Kim, Kyung-Jin Rhim, Won-Seok Jang, Sun-Joo Lee, Yeonghoon Son, Seung-Sook Lee, Sunhoo Park, Sang Moo Lim 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 1 1-9 (9 pages)
In the present study, the detrimental effect of β-emission on pig skin was evaluated. Skin injury was modeled in mini-pigs by exposing the animals to 50 and 100 Gy of β-emission delivered by Ho patches. Clinicopathological and immunohistochemical changes in exposed skin were monitored for 18 weeks after β-irradiation. Radiation induced desquamation at 2∼4 weeks and gradual repair of this damage was evident 6 weeks after irradiation. Changes in basal cell density... -
Effect of chronic lead intoxication on the distribution and elimination of amoxicillin in goats
Ahmed M. Soliman, Ehab A. Abu-Basha, Salah A. H. Youssef, Aziza M. Amer, Patricia A. Murphy, Catherine C. Hauck, Ronette Gehring 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 4 395-403 (9 pages)
A study of amoxicillin pharmacokinetics was conducted in healthy goats and goats with chronic lead intoxication. The intoxicated goats had increased serum concentrations of liver enzymes (alanine aminotransferase and γ-glutamyl transferase), blood urea nitrogen, and reactivated δ-aminolevulinic acid dehydratase compared to the controls. Following intravenous amoxicillin (10 mg/kg bw) in control and lead-intoxicated goats, elimination half-lives were 4.14 and 1.26 h, respectively. The volumes... -
Differential expression of thymic DNA repair genes in low-dose-rate irradiated AKR/J mice
Jin Jong Bong, Yu Mi Kang, Suk Chul Shin, Seung Jin Choi, Kyung Mi Lee, Hee Sun Kim 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 6 271-279 (9 pages)
We previously determined that AKR/J mice housed in a low-dose-rate (LDR) (Cs, 0.7 mGy/h, 2.1 Gy) γ-irradiation facility developed less spontaneous thymic lymphoma and survived longer than those receiving sham or high-dose-rate (HDR) (Cs, 0.8 Gy/min, 4.5 Gy) radiation. Interestingly, histopathological analysis showed a mild lymphomagenesis in the thymus of LDR-irradiated mice. Therefore, in this study, we investigated whether LDR irradiation could trigger the expression of thymic genes involved... -
Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Eun-Jin Choi, Chang-Hee Lee, Jae-Young Song, Hee-Jong Song, Choi-Kyu Park, Byounghan Kim, Yeun-Kyung Shin* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 2 115-124 (10 pages)
The high genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) has been an obstacle to developing an effective vaccine for porcine reproductive and respiratory syndrome (PRRS). This study was performed to assess the degree of genetic diversity among PRRSVs from Korean pig farms where wasting and respiratory syndrome was observed from 2005 to 2009. Samples from 786 farms were tested for the presence of PRRSV using reverse transcription PCR protocol. A total of 117 farms... -
A survey of porcine reproductive and respiratory syndrome among wild boar populations in Korea
Eun-Jin Choi, Chang-Hee Lee, Bang-Hun Hyun, Jae-Jo Kim, Seong-In Lim, Jae-Young Song, Yeun-Kyung Shin* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 7 377-383 (7 pages)
No information is currently available on porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the status of PRRS in wild boars was investigated. Blood samples were collected from 267 wild boars from eight provinces in Korea. Four of the samples tested (1.5%) were positive for PRRSV antibodies and eight (3.0%) were positive for antigens. Of the virus-positive samples, three and five samples were typed as containing European (EU,... -
Distribution of red-spotted grouper nervous necrosis virus (RGNNV) antigens in nervous and non-nervous organs of European seabass (Dicentrarchus labrax) during the course of an experimental challenge
Benjamin Lopez-Jimena, Esther Garcia-Rosado, Kim Dawn Thompson, Alexandra Adams, Carlos Infante, Juan Jose Borrego, Maria del Ca 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 4 355-362 (8 pages)
The distribution of red-spotted grouper nervous necrosis virus (RGNNV) antigens was examined by immunohistochemistry in the nervous and non-nervous organs of juvenile European seabass (Dicentrarchus labrax) during the course of an intramuscular infection. Histological changes resulting from the infection were evaluated from 3 days to 2 months post-infection. The specific antibody response was also studied 2 months post-challenge. Viral proteins were present throughout the experimental period in... -
Sedative and analgesic effects of intravenous xylazine and tramadol on horses
Jong-pil Seo, Won-gyun Son, Sujin Gang, Inhyung Lee* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 12 281-286 (6 pages)
This study was performed to evaluate the sedative and analgesic effects of xylazine (X) and tramadol (T) intravenously (IV) administered to horses. Six thoroughbred saddle horses each received X (1.0 mg/kg), T (2.0 mg/kg), and a combination of XT (1.0 and 2.0 mg/kg, respectively) IV. Heart rate (HR), respiratory rate (RR), rectal temperature (RT), indirect arterial pressure (IAP), capillary refill time (CRT), sedation, and analgesia (using electrical stimulation and pinprick) were measured... -
The critical time of avian leukosis virus subgroup J-mediated immunosuppression during early stage infection in specific pathogen-free chickens
Feng Wang, Xiaowei Wang, Hongbo Chen, Jianzhu Liu, Ziqiang Cheng* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 6 235-241 (7 pages)
The critical time of avian leukosis virus subgroup J (ALV-J)-mediated immunosuppression was determined by body weight, relative immune organ weight, histopathology, and presence of group specific antigen and antibodies in specific pathogen-free (SPF) chickens. CD4 and CD8 cell activity in the spleen, total and differential leukocyte counts in blood, and viral RNA levels in spleen were measured. Significant growth suppression was observed in the two ALV-J-infected groups. A strong immune response... -
Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey
G?khan Inat*, Belgin Siriken 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 7 321-326 (6 pages)
This study was conducted to investigate the presence of Escherichia (E.) coli O157 and E. coli O157:H7 and stx1 and stx2 genes on cattle carcasses and in rectal samples collected from Samsun Province of Turkey. A total of 200 samples collected from cattle carcasses and the rectal contents of 100 slaughtered cattle from two commercial abattoirs were tested using the immunomagnetic separation technique and multiplex PCR methods. E. coli O157 and E. coli O157:H7 were detected in 52 of the 200... -
Antimutagenic and anticarcinogenic effect of methanol extracts of Petasites japonicus Maxim leaves
Hwan-Goo Kang, Sang-Hee Jeong*, †, Joon-Hyoung Cho 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 8 51-58 (8 pages)
The methanol extract from the leaves of Petasites japonicus Maxim (PJ) was studied for its (anti-)mutagenic effect with the SOS chromotest and reverse mutation assay. The (anti-)carcinogenic effects were evaluated by the cytotoxicity on human cancer line cells and by the function and the expression of gap junctions in rat liver epithelial cell. PJ extracts significantly decreased spontaneous β-galactosidase activity and β-galactosidase activity induced by a mutagen, ICR, in Salmonella (S.)... -
Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes
Eunsong Lee, Mohammad Musharraf Uddin Bhuiyan, Hiroyuki Watanabe, Kohji Matsuoka, Yoshihiro Fujise, Hajime Ishikawa, Yutaka Fuku 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 2 285-292 (8 pages)
In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their developmental capacity by investigating the effect of osmolarity and macromolecules in the culture medium on the in vitro developmental capacity. Hybrid embryos produced by the electrofusion of fetal whale fibroblasts with enucleated porcine oocytes were cultured in modified porcine zygote medium-3... -
Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-g
Jeong Ho Park, Haan Woo Sung, Byung Il Yoon, Hyuk Moo Kwon* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 6 131-140 (10 pages)
The aim of this study was to examine the efficacy of in ovo prime-boost vaccination against infectious bursal disease virus (IBDV) using a DNA vaccine to prime in ovo followed by a killed-vaccine boost post hatching. In addition, the adjuvant effects of plasmid-encoded chicken interleukin-2 and chicken interferon-g were tested in conjunction with the vaccine. A plasmid DNA vaccine (pcDNA-VP243) encoding the VP2, VP4, and VP3 proteins of the very virulent IBDV (vvIBDV) SH/92 strain was injected... -
In vitro and in vivo gene therapy with CMV vector-mediated presumed dog β-nerve growth factor in pyridoxine-induced neuropathy dogs
Jin-Young Chung, Jung-Hoon Choi, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Sang-Koo Lee, Hwa-Young Youn* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 5 367-374 (8 pages)
Due to the therapeutic potential of gene therapy for neuronal injury, many studies of neurotrophic factors, vectors, and animal models have been performed. The presumed dog β-nerve growth factor (pdβ-NGF) was generated and cloned and its expression was confirmed in CHO cells. The recombinant pdβ-NGF protein reacted with a human β-NGF antibody and showed bioactivity in PC12 cells. The pdβb-NGF was shown to have similar bioactivity to the dog β-NGF. The recombinant pdβ-NGF plasmid was... -
Mitral valve prolapse in Cavalier King Charles Spaniel:A review and case study
Changbaig Hyun 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 10 67-73 (7 pages)
A 5 year-old spayed female Cavalier King Charles Spaniel was presented after a 3- to 5-day onset of severe respiratory distress. The dog also had a history of several episodes of syncope prior to presentation. A comprehensive diagnostic investigation revealed a midsystolic click sound on cardiac auscultation, signs of left sided cardiac enlargement in ECG and thoracic radiography, mitral valvular leaflet protrusion into left the atrium, decreased E-point-to septal separation (EPSS) and mitral... -
Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application
Deog Yong Lee, Young Wook Cho, Sang Gyun Kang, Sung Jae Shin, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 9 337-343 (7 pages)
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and accurate quantification method previously used with ovine, rat, and human IL-6 proteins. However, it has never been reported to quantify porcine IL-6 protein using capture ELISA. In this study, we generated and characterized a set of IgY and mono-specific polyclonal antibodies to recombinant porcine IL-6 (rpIL-6),... -
Application of biotechnological tools for coccidia vaccine development
Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 1 279-288 (10 pages)
Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of host-parasite interactions and...
