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Development of PCR-Based Molecular Marker for Detection of Xanthomonas campestris pv. campestris Race 6, the Causative Agent of Black Rot of Brassicas
Khandker Shazia Afrin, Md Abdur Rahim, Mehede Hassan Rubel, Jong-In Park, Hee-Jeong Jung, Hoy-Taek Kim, Ill-Sup Nou 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 4 418-427 (10 pages)
(SCAR) markers (i.e., XccR6-60 and XccR6-67) for the detection of Xcc race 6 via re-alignment of the complete genome sequences of Xcc races/ strains/pathovars. The specificity of SCAR primer sets was verified by mean of PCR amplification using the genomic DNA template of Xcc races/strains/pathovars and two other plant infecting bacterial strains. The PCR result revealed that the XccR6-60 and XccR6-67 primer sets amplified 692-bp and 917-bp DNA fragments, respectively, specifically from race 6,... -
Race- and Isolate-specific Molecular Marker Development through Genome- Realignment Enables Detection of Korean Plasmodiophora brassicae Isolates, Causal agents of Clubroot Disease
Ji-Yun Jeong, Arif Hasan Khan Robin, Sathishkumar Natarajan, Rawnak Laila, Hoy-Taek Kim, Jong-In Park, Ill-Sup Nou 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 6 506-513 (8 pages)
markers were specific to Hoengseong isolate and ZJ1-56 and ZJ1-64 markers were specific to Pyeongchang isolate (race 4, pathotype 3). The PCR-based sequence characterized amplified region (SCAR) markers developed in this study are able to detect five Korean isolates of P.brassicae. These markers can be utilized in identifying four Korean P. brassicae isolates from different regions. Additional effort is required to develop race- and isolate-specific markers for the remaining Korean isolates. -
PCR-Based Assay for Rapid and Specific Detection of the New Xanthomonas oryzae pv. oryzae K3a Race Using an AFLP-Derived Marker
Song. Eun-Sung, Kim. Song-Yi, Noh. Tae-Hwan, Cho. Heejung, Chae. Soo-Cheon, Lee. Byoung-Moo 한국미생물생명공학회 Journal of microbiology and biotechnology 8 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2014, Vol.24 No.6 732-739 (8 pages)
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Immunohistochemical detection of Prion protein (PrP-Sc) and epidemiological study of BSE in Korea
Hye Cheong Koo, Yong Ho Park, Byeong-Chun Lee, Chanhee Chae, Katherine I. O'Rourke, Timothy V. Baszler 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 4 25-31 (7 pages)
Though the aetiology of transmissible spongiform encephalopathies (TSEs) remains uncertain, proteinase resistant prion protein (PrP-Sc), a converted form of the normal cellular prion protein (PrP-C), accumulates in the lysosome of cells of the nervous systems of animals with TSEs. In this study, clinical and epidemiological examinations of bovine spongiform encephalopathy (BSE) were conducted in Korea. During the investigated period, none of the cattle exhibited typical clinical signs of BSE,... -
Detection of Blackleg Resistance Gene Rlm1 in Double-Low Rapeseed Accessions from Sichuan Province, by Kompetitive Allele-Specific PCR
Liang Chai, Jinfang Zhang, Wannakuwattewaduge Gerard Dilantha Fernando, Haojie Li, Xiaoqin Huang, Cheng Cui, Jun Jiang, Benchuan Zheng, Yong Liu, Liangcai Jiang 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 10 194-199 (6 pages)
Blackleg is a serious disease in Brassica plants, causing moderate to severe yield losses in rapeseed worldwide. Although China has not suffered from this disease yet (more aggressive Leptosphaeria maculans is not present yet), it is crucial to take provisions in breeding for dis- ease resistance to have excellent blackleg-resistant cul- tivars already in the fields or in the breeding pipeline. The most efficient strategy for controlling this disease is breeding plants with identified resistance... -
Simultaneous Detection of Three Bacterial Seed-Borne Diseases in Rice Using Multiplex Polymerase Chain Reaction
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 6호 11 575-579 (5 pages)
Burkholderia glumae (bacterial grain rot), Xanthomonas oryzae pv. oryzae (bacterial leaf blight), and Acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. Based on the 16S and 23S rDNA sequences for A. avenae subsp. avenae and B. glumae, and transposase A gene sequence for X. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for B. glumae, 490 bp for X. oryzae, and 290 bp for A. avenae subsp. avenae with the 63°C as an... -
Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 4 123-131 (9 pages)
a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced genomes of the C. michiganensis subspecies and to identify an appropriate gene for the development of a subspecies-specific PCR primer set (Cms89F/R).... -
First Report of Bacterial Wilt Caused by Ralstonia solanacearum Biovar 2 Race 1 on Tomato in Egypt
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 3호 8 299-303 (5 pages)
sections showed a brown discoloration in the vascular tissues. Bacteria were isolated on triphenyl tetrazolium chloride medium and fifteen isolates shown typical morphological and cultural characteristics were confirmed as Ralstonia solanacearum biovar 2 race 1. Pathogenicity tests showed that all isolates proved to be pathogenic to tomato plants, varied from 52 to 97% wilting. This is the first report of R. solanacearum biovar 2 race 1 causing bacterial wilt in tomato crop in Egypt. -
Occurrence of Tropical Race 4 of Fusarium oxysporum f. sp. cubense in Indonesia
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 10 280-284 (5 pages)
groups representing strains that belong to race 1, 2, and 4. Three isolates formed heterokaryons with nitM testers belong to race 1, 11 isolates with race 4, and the rest did not form heterokaryons with all nitM testers used. F. oxysporum f. sp. cubense tropical race 4 specific primer pair was used to amplify a 1400 bp fragment of tropical race 4 DNA. Seven isolates (Bnt2, Mln1, Srg1, Bgl3, Bgl6, Lmp1, and Kjg1) produced the 1400 bp amplification product were therefore tropical race 4. -
Specific PCR Detection of Four Quarantine Fusarium Species in Korea
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 16 409-416 (8 pages)
A set of nested primers for each race was designed to confirm the PCR results. Similarly, two primer sets derived from RPB2 successfully amplified specific fragments from five F. stilboides isolates grouped within a single phylogenetic clade. A specific TEF1 primer set amplified a DNA fragment from only four of the 12 F. redolens strains examined, which were grouped within a single phylogenetic clade. All of the F. semitectum var. majus isolates could be specifically detected with a single RPB2... -
Detection of Pathotypes and Genetic Diversity of Cercospora beticola
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 2 306-312 (7 pages)
The pathotypes of Cercospora beticola, causal agent of sugar beet leaf spot disease, were identified by application of pathogenicity test using 100 isolates obtained from the provinces with intensive sugar beet cultivation. For the identification of pathotypes, five sugar beet cultivars were used each with different resistance factors. Cultivar reactions were determined by inoculation of cultivars with the isolates under controlled conditions and measuring disease severity on the 15th day... -
Identification and characterization of atypical porcine pestivirus genomes in newborn piglets with congenital tremor in China
Hanqin Shen, Xiangcong Liu, Pengfei Zhang, Lei Wang, Yanling Liu, Leyi Zhang, Pengshuai Liang, Changxu Song 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 17 468-471 (4 pages)
Recently, a novel atypical porcine pestivirus (APPV) in pig was reported. In this study, two APPV strains, APPV-China/GZ01/2016 (GZ01) and APPV-China/GD-SD/2016 (GD-SD), were identified in two newborn piglet herds with congenital tremor from China. The open reading frame of the two strains shared an 83.5% nucleotide identity. Phylogenetically, the APPV strains were placed into two groups: GZ01 belonged to group I and GD-SD belonged to group II. A high viral load was detected in the cerebellum... -
Infections by pathogens with different transmission modes in feral cats from urban and rural areas of Korea
Jusun Hwang, Nicole Gottdenker, Dae-Hyun Oh, Hang Lee, Myung-Sun Chun 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 14 541-545 (5 pages)
In this study, we examine prevalences of three infectious pathogens with different transmission modes (Bartonella henselae, hemoplasma, and Toxoplasma gondii) in feral cats from urban and rural habitats. Infection status of the three pathogens in blood samples (n = 117) was determined through molecular or serological diagnostic methods. Overall prevalence of hemoplasma, Toxoplasma gondii, and Bartonella henselae was 47.9%, 50%, and 35.7%, respectively. Comparing the two habitats, only... -
Isolation and characterization of antimicrobial-resistant Escherichia coli from national horse racetracks and private horse-riding courses in Korea
Yeon Soo Chung, Jae Won Song, Dae Ho Kim, Sook Shin, Young Kyung Park, Soo Jin Yang, Suk Kyung Lim, Kun Taek Park, Yong Ho Park 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 9 199-206 (8 pages)
Limited information is available regarding horse-associated antimicrobial resistant (AR) Escherichia (E.) coli. This study was designed to evaluate the frequency and characterize the pattern of AR E. coli from healthy horse-associated samples. A total of 143 E. coli (4.6%) were isolated from 3,078 samples collected from three national racetracks and 14 private horse-riding courses in Korea. Thirty of the E. coli isolates (21%) showed antimicrobial resistance to at least one antimicrobial agent,... -
Classical natural ovine scrapie prions detected in practical volumes of blood by lamb and transgenic mouse bioassays
Rohana P. Dassanayake, Thomas C. Truscott, Dongyue Zhuang, David A. Schneider, Sally A. Madsen-Bouterse, Alan J. Young, James B. 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 7 179-186 (8 pages)
convenient for mass live animal scrapie testing. Currently approved techniques, however, have their own detection limits. Novel detection methods may soon offer a non-animal-based, rapid platform with detection sensitivities that rival the prion bioassay. In anticipation, we sought to determine if diseased animals could be routinely identified with a bioassay using B lymphocytes isolated from blood sample volumes commonly collected for diagnostic purposes in small ruminants. Scrapie transmission... -
Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene
Yuqiang Cheng, Yingjie Sun, Hengan Wang, Shuduan Shi, Yaxian Yan, Jing Li, Chan Ding, Jianhe Sun 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 6 37-46 (10 pages)
Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial sequences of myeloid differentiation factor 88, 2’-5’-oligoadenylate synthetase (OAS), and myxovirus resistance (Mx) genes from duck. dTLR5 mRNA was highly expressed in the... -
Betulinic acid prevents alcohol-induced liver damage by improving the antioxidant system in mice
Jine Yi*, Wei Xia, Jianping Wu, Liyun Yuan, Jing Wu, Di Tu, Jun Fang, Zhuliang Tan* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 16 141-148 (8 pages)
Betulinic acid (BA), a pentacyclic lupane-type triterpene, has a wide range of bioactivities. The main objective of this work was to evaluate the hepatoprotective activity of BA and the potential mechanism underlying the ability of this compound to prevent liver damage induced by alcohol in vivo. Mice were given oral doses of BA (0.25, 0.5, and 1.0 mg/kg) daily for 14 days, and induced liver injury by feeding 50% alcohol orally at the dosage of 10 ml/kg after 1 h last administration of BA. BA... -
Identification of differentially expressed genes in gauze-exposed omentum of dogs using differential display RT-PCR
Md. Mizanur Rahman, Yongsun Kim, Ye-Eun Byeon, Hak-Hyun Ryu, Wan Hee Kim, Mahmuda Umme Rayhan, Oh-Kyeong Kweon* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 8 167-173 (7 pages)
Molecular mechanisms governing peritonitis caused by the presence of aseptic gauze have remained unclear. To identify the genes involved, sterile gauze-exposed omentum was collected at 0, 6, 12, 24, and 48 h intervals, and analyzed by differential display RT(reverse transcription)-PCR. Among over 1,200 bands, 230 bands were found differentially expressed. These bands represented the fragment sizes of approximately 200 to 1,500 bp. The eight fragments were expressed differentially in the... -
Development of monoclonal antibodies against the abnormal prion protein isoform (PrP) associated with chronic wasting disease (CWD)
Hyun-Jeong Jeong, Nak-Hyung Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Kun-Ho Seo, Dong-Woon Kim, Yong-Sun Kim, In-So 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 14 429-432 (4 pages)
Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to... -
Serosurveillance for Japanese encephalitis, Akabane, and Aino viruses for Thoroughbred horses in Korea
Dong-Kun Yang*, Byoung-han Kim, Chang-Hee Kweon, Jin-Ju Nah, Hyun-Joo Kim, Kyung-Woo Lee, Young-Jin Yang, Kyu-Whan Mun 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 7 381-386 (6 pages)
Recent global warming trends may have a significant impact on vector-borne viral diseases, possibly affecting vector population dynamics and disease transmission. This study measured levels of hemagglutination-inhibition (HI) antibodies against Japanese encephalitis virus (JEV) and neutralizing antibodies against Akabane virus (AKAV) and Aino virus (AINV) for Thoroughbred horses in Korea. Blood samples were collected from 989 racehorses in several provinces, between October 2005 and March 2007.... -
Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp
Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 5 131-137 (7 pages)
A normal prion protein (PrPc) is converted to a proteaseresistant isoform by an apparent self-propagating activity in transmissible spongiform encephalopathy, a neurodegenerative disease. The cDNA encoding open reading frame (ORF) of the bovine prion protein gene (Prnp) was cloned from Korean cattle by PCR, and was transfected into Chinese hamster ovary (CHO-K1) cells using lipofectamine. The gene expression of the cloned cDNA was confirmed by RT-PCR and Western blotting with the monoclonal... -
Cardiac Damage Biomarkers Following a Triathlon in Elite and Non-elite Triathletes
Chan-HoPark, Kwi-BaekKim, JinHan, Jin-GooJi, Yi-SubKwak 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2014, Vol.18 No.5 10 419-423 (5 pages)
after a triathlon race in elite and non-elite athlete groups. Fifteen healthy men participated in the study. Based on performance, they were divided into elite athlete group (EG: n＝7) and non-elite athlete group (NEG: n＝8). Participants’ blood samples were obtained during four periods: before, immediately, 2 hours and 7 days after finishing the race. creatine kinase (CK), creatine kinase-myoglobin (CK-MB), myoglobin, and lactate dehydrogenase (LDH) were significantly increased in both groups... -
Comparison of Interleukin-8 Levels in Long-Distance Runners and Healthy Sedentary Non-Athletic Control Subjects
Young-OhShin, Jun-SangBae, Ji-WonMin, Jeong-BeomLee, Jung-KyuKim, Young-JuSong, Hun-MoYang, Young-KiMin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2007, Vol.11 No.6 6 263-268 (6 pages)
We have previously demonstrated that the level of leukocytes and neutrophils significantly increased immediately and 30 min after exercise. Interleukin-8 (IL-8) is an inflammatory cytokine that acts as a chemokine on neutrophils. In the present study, we evaluated the correlation between the number of neutrophils and leukocytes, and between the number of neutrophils and plasma IL-8 level. Long-distance trained runners (TRs, n = 10) and untrained sedentary control subjects (SEDs, n = 10) ran for... -
정신분열병 환자의 비정형 항정신병약물 반응과 세로토닌 2A 및 도파민 D3 수용체 유전자 다형성의 관련연구
김병수, 주연호, 김창윤, 박지홍, 송규영, 한오수 대한신경정신의학회 신경정신의학 12 Pages
대한신경정신의학회 신경정신의학 2002, 제 41권 제 3호 4 409-420 (12 pages)
연구목적： 본 연구는 정신분열병 환자군을 대상으로 비정형 항정신병 약물에 대한 반응과 도파민 D3 수용체 유전자 및 세로토닌 2A 수용체 유전자의 관련성을 확인하기 위해 시행되었다. 방 법： 서울중앙병원 정신과에서 치료 받은 환자 중에서 정신장애의 진단 및 통계 편람 제 4 판(DSM-Ⅳ) 의 정신분열병 진단 기준에 부합되는 환자 75명(남자 39명, 여자 36명)을 대상으로 하였다. 모든 대 상 환자들(혹은 보호자)은 본 연구의 목적과 과정에 대한 설명을 들은 후 서면 동의를 하였다. 약물 반응을 평가하기 위해서 CGI(Clinical... -
중·고등학교 청소년의 자살사고 및 자살시도의 유병률과 자살시도의 위험요인에 관한 연구
조성진, 전홍진, 김장규, 서동우, 김선욱, 함봉진, 서동혁, 정선주, 조맹제 대한신경정신의학회 신경정신의학 14 Pages
대한신경정신의학회 신경정신의학 2002, 제 41권 제 6호 18 1142-1155 (14 pages)
연구목적： 지역사회에 거주하고 있는, 중·고등학교 청소년들을 대상으로 자살 사고 및 자살시도의 빈도, 성별 과 연령(13~15세 vs 16~18세)에 따른 자살사고 및 시도의 빈도에 차이가 있는지 여부, 자살사고 및 자살시도가 있는 청소년의 특징, 그리고 자살시도의 위험요인을 알아보고자 하였다. 방 법： 부천시에 소재하고 있는 중학교 24개교 및 고등학교 20개교의 청소년 71,102명중에서 무작위 집 락 표본 추출범으로 청소년 2,203명(남자 1,105명, 여자 1,098명)을 조사대상으로 선정하였다. 자살 시도 및 자살사고의 정의에 관한... -
Evaluation of Durum Wheat Genotypes for Resistance against Root Rot Disease Caused by Moroccan Fusarium culmorum Isolates
Jamila Bouarda, Filippo M. Bassi, Hugh Wallwork, Mohammed Benchacho, Mustapha Labhilili, Ilyass Maafa, Aicha El Aissami, Fatiha Bentata 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 1호 1 1-11 (11 pages)
Fusarium culmorum is one of the most important causal agents of root rot of wheat. In this study, 10 F. culmorum isolates were collected from farms located in five agro-ecological regions of Morocco. These were used to challenge 20 durum wheat genotypes via artificial inoculation of plant roots under controlled conditions. The isolate virulence was determined by three traits (roots browning index, stem browning index, and severity of root rot). An alpha-lattice design with three replicates was... -
Characterization of Xanthomonas citri pv. glycines Population Genetics and Virulence in a National Survey of Bacterial Pustule Disease in Korea
In-Jeong Kang, Kyung Seok Kim, Gwyn A. Beattie, Hyunjung Chung, Sunggi Heu, Ingyu Hwang 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 14 652-661 (10 pages)
Xanthomonas citri pv. glycines (Xcg) is a major patho- gen of soybean (Glycine max) in South Korea, de- spite the availability of soybean varieties with some resistance. We conducted a nationwide survey of the incidence and severity of bacterial pustule caused by Xcg. The percentage of infected fields was 7% to 17% between 2015 and 2017. We characterized the diver- sity of a nationwide collection of 106 Xcg isolates based on avrBs3 banding patterns. The isolates fell into 11 groups, each... -
Genetic Diversity of Orobanche cumana Populations in Serbia
?arko Ivanovi?, Dragana Marisavljevi?, Radovan Marinkovi?, Petar Mitrovi?, Jovana Blagojevi?, Ivan Nikoli?, Danijela Pavlovi? 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 2 512-520 (9 pages)
In this study, we report genetic characterization of Oro- banche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analy- ses, rbcL gene sequences... -
Genetic Diversity and Population Structure of the Xanthomonas campestris pv. campestris Strains Affecting Cabbages in China Revealed by MLST and Rep-PCR Based Genotyping
Guo Chen, Congcong Kong, Limei Yang, Mu Zhuang, Yangyong Zhang, Yong Wang, Jialei Ji, Zhiyuan Fang, Honghao Lv 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 5호 7 476-488 (13 pages)
Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot for cruciferous vegetables worldwide, especially for the cole crops such as cabbage and cauliflower. Due to the lack of resistant cabbage cultivars, black rot has brought about considerable yield losses in recent years in China. Understanding of the pathogen features is a key step for disease prevention, however, the pathogen diversity, population structure, and virulence are largely unknown. In this study, we studied... -
Isolate Virulence and Cultivar Response in the Winter Wheat: Pyrenophora tritici-repentis (Tan Spot) Pathosystem in Oklahoma
Kazi A. Kader, Robert M. Hunger, Mark E. Payton 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 4호 3 339-346 (8 pages)
Prevalence of tan spot of wheat caused by the fungus Pyrenophora tritici-repentis has become more prevalent in Oklahoma as no-till cultivation in wheat has in- creased. Hence, developing wheat varieties resistant to tan spot has been emphasized, and selecting pathogen isolates to screen for resistance to this disease is critical. Twelve isolates of P. tritici-repentis were used to inocu- late 11 wheat cultivars in a greenhouse study in split- plot experiments. Virulence of isolates and cultivar... -
Spatial and Temporal Genetic Diversity and Population Structure of Hemileia vastatrix from Peruvian Coffee Plantations
Cinthia Quispe-Apaza, Roberto Mansilla-Samaniego 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 7 280-290 (11 pages)
Population genetic studies of Hemileia vastatrix have been conducted in order to describe the evolutionary dynamics of the pathogen and the disease epidemiology as consequence of changes in disease management and host distribution occurred in Peru after the 2013 epi- demic. These analyses were performed by sequencing the internal transcribed spacers of the nuclear ribosom- al DNA (rDNA-ITS) of H. vastatrix collected from two coffee growing areas in 2014 and 2018. H. vastatrix pop- ulation showed... -
Complete Genome Sequencing and Infectious cDNA Clone Construction of Soybean Mosaic Virus Isolated from Shanxi
Defu Wang, Liyan Cui, Li Zhang, Zhennan Ma, Yanbing Niu 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 7 162-172 (11 pages)
Soybean mosaic virus (SMV) is the predominant viral pathogen that affects the yield and quality of soy- bean. The natural host range for SMV is very narrow, and generally limited to Leguminosae. However, we found that SMV can naturally infect Pinellia ternata and Atractylodes macrocephala. In order to clarify the molecular mechanisms underlying the cross- family infection of SMV, we used double-stranded RNA extraction, rapid amplification of cDNA ends polymerase chain reaction and Gibson... -
Necrotrophic Fungus Pyrenophora tritici-repentis Triggers Expression of Multiple Resistance Components in Resistant and Susceptible Wheat Cultivars
Ethan J. Andersen, Madhav P. Nepal, Shaukat Ali 한국식물병리학회 The Plant Pathology Journal 16 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 2 99-114 (16 pages)
resistant and susceptible wheat cultivars after infection with Ptr ToxA-producing race 2 and direct infiltration with Ptr ToxA proteins. Greenhouse experiments included ex- posure of the wheat cultivars to pathogen inoculum or direct infiltration of leaf tissue with Ptr-ToxA protein isolate. Samples from the experiments were subjected to RNA sequencing. Results showed that ToxA RNA sequences were first detected in samples collected eight hours after treatments indicating that upon Ptr con- tact... -
Genetic and Pathogenic Characterization of Bacterial Wilt Pathogen, Ralstonia pseudosolanacearum (Ralstonia solanacearum Phylotype I), on Roses in Korea
Ingyeong Lee, Yeong Son Kim, Jin-Won Kim, Duck Hwan Park 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 6 440-449 (10 pages)
as race 1 using race-specific primers, and were confirmed as biovar 3 due to their ability to utilize carbon sources. Multiplex PCR using phylotype discriminating specific primers identified the 17 isolates as phylotype I. Sequevar comparison with reference sequevars using the sequences of the egl, mutS, and fliC genes, and only the egl gene, revealed that the strains evaluated in this study corresponded to sequevar I-33. The pathogenicity in roses differed depending on the rose cultivars. The... -
Effect of Introducing Chitinase Gene on the Resistance of Tuber Mustard against White Mold
Seyedmohammadreza Ojaghian, Ling Wang, Guan-Lin Xie 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 4호 8 378-383 (6 pages)
The objective of this research was introduction of chit42 to tuber mustard plants through Agrobacterium- mediated transformation against white mold caused by Sclerotinia sclerotiorum. The binary plasmid pGisPEC1 was used in this study. Polymerase chain reaction analysis detected the transgene in 27 transformants with a transformation efficiency of 6.9%. Southern blot test was used to assess the copy number of transgene in tuber mustard plants. One, two, two, and two chit42- related bands were... -
Contribution of the murI Gene Encoding Glutamate Racemase in the Motility and Virulence of Ralstonia solanacearum
Kihyuck Choi, Geun Ju Son, Shabir Ahmad, Seung Yeup Lee, Hyoung Ju Lee, Seon-Woo Lee 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 4호 6 355-363 (9 pages)
Bacterial traits for virulence of Ralstonia solanacearum causing lethal wilt in plants were extensively studied but are not yet fully understood. Other than the known virulence factors of Ralstonia solanacearum, this study aimed to identify the novel gene(s) contributing to bacterial virulence of R. solanacearum. Among the transposon-inserted mutants that were previously generated, we selected mutant SL341F12 strain produced exopolysaccharide equivalent to wild type strain but showed reduced... -
A LysM Domain-Containing Protein LtLysM1 Is Important for Vegetative Growth and Pathogenesis in Woody Plant Pathogen Lasiodiplodia theobromae
Dulanjalee Lakmali Harishchandra, Wei Zhang, Xinghong Li, Kandawatte Wedaralalage Thilini Chethana, Kevin David Hyde, Siraprapa Brooks, Jiye Yan, Junbo Peng 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 4호 3 323-334 (12 pages)
Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence... -
Screening and Application of Bacillus Strains Isolated from Nonrhizospheric Rice Soil for the Biocontrol of Rice Blast
Yuexia Sha, Qingchao Zeng, Shuting Sui 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 3호 4 231-243 (13 pages)
Rice blast, caused by Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. The aim of this study was to screen bacterial isolates to efficiently prevent the occurrence of rice blast. A total of 232 bacterial isolates were extracted from nonrhizospheric rice soil and were screened for antifungal activity against M. oryzae using a leaf segment assay. Strains S170 and S9 showed significant antagonistic activity against M. oryzae in vitro and in leaf disk assays, and... -
Genetic Diversity Studies and Identification of Molecular and Biochemical Markers Associated with Fusarium Wilt Resistance in Cultivated Faba Bean (Vicia faba)
Amer F. Mahmoud, Bahaa E. S. Abd El-Fatah 한국식물병리학회 The Plant Pathology Journal 18 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 2 11-28 (18 pages)
Faba bean (Vicia faba L.) is one of the most important legume crops in Egypt. However, production of faba bean is affected by several diseases including fungal diseases. Fusarium wilt incited by Fusarium oxysporum Schlecht. was shown to be the most common wilt disease of faba bean in Assiut Governorate. Evaluation of 16 faba bean genotypes for the resistance to Fusarium wilt was carried out under greenhouse conditions. Three molecular marker systems (inter-simple sequence repeat [ISSR], sequence... -
Identification of New Isolates of Phytophthora sojae and the Reactions of Korean Soybean Cultivars Following Hypocotyl Inoculation
In Jeong Kang, Sunjoo Kang, Ik Hyun Jang, Yun Woo Jang, Hyung Kwon Shim, Sunggi Heu, Sungwoo Lee 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 16 698-704 (7 pages)
Phytophthora root and stem rot (PRSR) caused by Phytophthora sojae is one of the most destructive diseases of soybean. PRSR recently became an issue as soybean cultivation in paddy fields increased in South Korea. The management of PRSR mainly involves Rgene– mediated resistance, however, little is known about the resistance in Korean cultivars. Major Korean soybean cultivars were investigated for the presence or absence of R-gene-mediated resistance to four P. sojae isolates, two of which... -
A Biostimulant Preparation of Brown Seaweed Ascophyllum nodosum Suppresses Powdery Mildew of Strawberry
Sruti Bajpai, Pushp Sheel Shukla, Samuel Asiedu, Kris Pruski, Balakrishnan Prithiviraj 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 2 406-416 (11 pages)
Strawberry, an important fruit crop, is susceptible to a large number of pathogens that reduce fruit quality and productivity. In this study, the effect of a biostimulant prepared from Ascophyllum nodosum extract (ANE) (0.1%, 0.2%, and 0.3%) was evaluated on powdery mildew progression under greenhouse and field conditions. In the greenhouse, application of 0.2% ANE showed maximum reduction in powdery mildew progression as compared to the control. Forty-eight hour post-inoculation, foliar spray... -
Culturing Simpler and Bacterial Wilt Suppressive Microbial Communities from Tomato Rhizosphere
Nazish Roy, Kihyuck Choi, Raees Khan, Seon-Woo Lee 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 4호 8 362-374 (13 pages)
Plant phenotype is affected by a community of associated microorganisms which requires dissection of the functional fraction. In this study, we aimed to culture the functionally active fraction of an upland soil microbiome, which can suppress tomato bacterial wilt. The microbiome fraction (MF) from the rhizosphere of Hawaii 7996 treated with an upland soil or forest soil MF was successively cultured in a designed modified M9 (MM9) medium partially mimicking the nutrient composition of tomato... -
Variation in the Resistance of Japanese Soybean Cultivars to Phytophthora Root and Stem Rot during the Early Plant Growth Stages and the Effects of a Fungicide Seed Treatment
Hajime Akamatsu, Masayasu Kato, Sunao Ochi, Genki Mimuro, Jun-ichi Matsuoka, Mami Takahashi 한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 3호 4 219-233 (15 pages)
Soybean cultivars susceptible to Phytophthora root and stem rot are vulnerable to seed rot and damping-off of seedlings and young plants following an infection by Phytophthora sojae. In this study, the disease responses of Japanese soybean cultivars including currently grown main cultivars during the early growth stages were investigated following infections by multiple P. sojae isolates from Japanese fields. The extent of the resistance to 17 P. sojae isolates after inoculations at 14, 21, and... -
Development and Validation of a Perfect KASP Marker for Fusarium Head Blight Resistance Gene Fhb1 in Wheat
Lovepreet Singh, James A Anderson, Jianli Chen, Bikram S Gill, Vijay K Tiwari, Nidhi Rawat 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 3호 2 200-207 (8 pages)
Fusarium head blight (FHB) is a devastating wheat disease with a significant economic impact. Fhb1 is the most important large effect and stable QTL for FHB resistance. A pore-forming toxin-like (PFT) gene was recently identified as an underlying gene for Fhb1 resistance. In this study, we developed and validated a PFTbased Kompetitive allele specific PCR (KASP) marker for Fhb1. The KASP marker, PFT_KASP, was used to screen 298 diverse wheat breeding lines and cultivars. The KASP clustering...
