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전체 선택해제
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본문검색 결과만 보기
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Biological Rhythm in Livestock
Piccione Giuseppe and Caola Giovanni 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 1 145-157 (13 pages)
The animal time structure is a basic fact of life, no matter if one wants to study it or not. The time-dependent, mostly rhythmic, and thus to a certain degree predictable, variations of biochemical and physiological functions and of sensitivity and resistance to many environmental agents are often quite large and offer not only new insight into animal physiology and pathology but also diagnostic possibilities and therapeutic advantages. Chronobiology, chronophysiology and its subspecialities,... -
Localization of Porcine Reproductive and Respiratory Syndrome Virus Infection in Boars by In Situ Riboprobe Hybridization
Jin-Ho Shin and Thomas W. Molitor 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 5 87-96 (10 pages)
The capability of porcine reproductive and respiratory syndrome virus (PRRSV) to be shed in semen for extended periods of time has been suggested to be a principal factor for viral transmission via insemination. In attempts to gain insights into the mechanism of PRRSV persistence in boars, tissue distribution and sites of viral infection were investigated by in situ hybridization (ISH) using digoxigenin-labeled RNA probe and the ISH results were compared with those of reverse... -
Assessment of Porcine Reproductive and Respiratory Syndrome Virus RNA Load in Sera and Tissues during Acute Infection
Jin-Ho Shin and Thomas W. Molitor 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 4 75-86 (12 pages)
Porcine reproductive and respiratory syndrome virus (PRRSV) RNA load in sera and tissues during acute phase of infection was evaluated using a PCR- based quantitative assay. More than 80% of infected pigs (21/25) showed the peak level of viral RNA concentrations in serum (up to 8.6 x 10(8) copies/ml) at day 5 postinfection (PI), and started to clear the virus from the systemic circulation thereafter. Regression analysis using the viral RNA concentrations in sera obtained from days 5 to 14 PI... -
Assessment of Replication and Virulence of Attenuated Pseudorabies Virus in Swine
T. J. Newby, D. P. Carter, K.-J. Yoon, M. W. Jackwood and P. A. Hawkins 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 1 61-66 (6 pages)
A nonclinical study was conducted to characterize the replication behavior of a modified live gE-deleted pseudorabies virus (PRV MS) in swine and potential for reversion to virulence after animal passages. Two to 3 week-old weaned pigs, negative for PRV, were maintained in isolation and challenged by intranasal instillation. For the first passage, 6 pigs were given 1 mL of PRV MS (10(7.3)TCID(50)/mL) and 2 were necropsied at 3, 4 and 5 days post-inoculation (PI). Brain and secondary lymphoid... -
Genetic characterization of porcine circovirus - 2 field isolates from PMWS pigs
Jin H. Kim, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 6 31-39 (9 pages)
PMWS is a new emerging disease in swine herds worldwide. Field isolates of PCV-2, a putative major causative agent of PMWS, were isolated and genetically characterized. Viral genome of two field isolates (PC201DJ and PC201SS) from pigs showing typical PMWS was sequenced. The nucleotide sequence homology with other PCV-2 isolates was ranging from 95% to 99% in complete viral genomic sequence. The highly conserved nonanucleotide motif of replication origin was identical to that of other PCV-2... -
Characterization of the Recombinant Proteins of Porcine ircovirus Type 2 Field Isolate Expressed in the Baculovirus System
Yu Na Kim, Jin Hyun Kim, Kyoung Soo Kang, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 4 19-23 (5 pages)
Porcine circovirus (PCV) type2 was isolated using primary porcine kidney cells from lymph node of piglets with typical PMWS. The presence of the virus was identified by PCR using primers specific to PCV type2. The ORFs 1 and 2 were amplified by PCR using primers corresponding to the target genes of the PCV type 2. Cloned genes were inserted into the baculovirus expression vector and PCV recombinant proteins were expressed using baculovirus expression system. Recombinant protein expression was... -
Comparative study of endocrine cells in the principal pancreatic islets of two teleosts, Silurus asotus (Siluridae) and Siniperca scherzeri (Centropomidae)
Jae-hyun Lee, Sae-kwang Ku, Ki-dae Park and Hyeung-sik Lee 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 1 75-80 (6 pages)
in the peripheral regions with low frequency in Siniperca scherzeri. Bovine PPimmunoreactive cells were found in the peripheral region and the mantle zone of the principal pancreatic islets with low and rare frequency, respectively in both species. In conclusion, the regional distribution and relative frequency of endocrine cells in the principal pancreatic islets of Silurus asotus showed general patterns similar to those of other teleostean fishes. But, some speciesdependent distributional... -
An immunohistochemical study on the pancreatic islets cells of the Mongolian gerbils, Meriones unguiculatus
Sae-kwang Ku, Hyeung-sik Lee, Ki-dae Park, Jae-hyun Lee 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 2 9-14 (6 pages)
In order to study the regional distribution and relative frequency of the immunoreactive endocrine cells in the pancreatic islets of the Mongolian gerbil, pancreatic sections of Meriones unguiculatus were immunostained using an immunohistochemical (PAP) method with four types of specific antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (PP). The pancreatic islets were subdivided into three portions (central region, mantle zone and peripheral region) according to... -
Using pig biliary system, in vivo propagation of Enterocytozoon bieneusi, an AIDS-related zoonotic pathogen
John Hwa Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 7 105-111 (7 pages)
A microsporidian parasite Enterocytozoon bieneusi is the most common microorganism recognized in AIDS patients, and slow scientific progress is attributed to our inability to propagate the parasite. We report upon the development of a system of propagation using the pig biliary system. The parasite spores were continuously detected in the bile samples post onset of spore shedding in the gall bladder, which suggests that this organism maintain persistent infection in the biliary system and that...
