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Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves
Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 9 57-64 (8 pages)
in tissue culture. The antibodies, thus obtained, were affinity-purified, characterized and coated onto Giemsastained Cowan-I strain of Staphylococcus aureus (S. aureus). The antibody-coated S. aureus were used in a coagglutination test to detect K99+ E. coli isolated from feces of diarrheic calves. The specificity of the test was validated against reference monoclonal antibodies used in co-agglutination tests, as well as in ELISA. Specificity of the monoclonal antibodies was also tested against... -
Estrogen receptor independent neurotoxic mechanism of bisphenol A, an environmental estrogen
Yoot Mo Lee, Min Jae Seong, Jae Woong Lee, Yong Kyung Lee, Tae Myoung Kim, Sang-Yoon Nam, Dae Joong Kim, Young Won Yun, Tae Seon 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 5 27-38 (12 pages)
effects. The cell vulnerability against BPA was not significantly different in the PC12 cells overexpressing ER-α and ER-β compared with PC12 cells expressing vector alone. In addition, there was no difference observed between BPA and 17-β estradiol, a well-known agonist of ER receptor in the induction of neurotoxic responses. Further study of the mechanism showed that BPA significantly activated extracellular signal-regulated kinase (ERK) but inhibited anti-apoptotic nuclear factor kappa B... -
Pharmacokinetics and bioavailability of doxycycline in ostriches (Struthio camelus) at two different dose rates
Ehab A. Abu-Basha, Nasir M. Idkaidek, Tareq M. Hantash 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 4 327-332 (6 pages)
The plasma concentration-time curves were examined using non-compartmental methods based on the statistical moment theory for only the higher dose. After IV administration, the elimination half-life (t1/2β), mean residence time (MRT), volume of distribution at the steady-state (Vss), volume of distribution (Vdarea) and total body clearance (ClB) were 7.67 ± 0.62 h, 6.68 ± 0.86 h, 0.86 ± 0.16 l/kg, 1.67 ± 0.52 l/kg and 2.51 ± 0.63 ml/min/kg, respectively. After IM and oral dosing, the mean... -
Cellular uptake of magnetic nanoparticle is mediated through energy-dependent endocytosis in A549 cells
Jun-Sung Kim, Tae-Jong Yoon, Kyeong-Nam Yu, Mi Suk Noh, Minah Woo, Byung-Geol Kim, Kee-Ho Lee, Byung-Hyuk Sohn, Seung-Bum Park, 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 3 321-326 (6 pages)
is the most likely route of exposure and lung cancer cells were also found to uptake magnetic nanoparticles rapidly in preliminary experiments. The lung cells were pretreated with different metabolic inhibitors. Our results revealed that low temperature disturbed the uptake of magnetic nanoparticles into the cells. Metabolic inhibitors also prevented the delivery of the materials into cells. Use of TEM clearly demonstrated that uptake of the nanoparticles was mediated through endosomes. Taken... -
Topical application of epidermal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis in rat
Young-Bae Kwon, Hyun-Woo Kim, Dae-Hyun Roh, Seo-Yeon Yoon, Rong-Min Baek, Jeum -Yong Kim, HaeYong Kweon, Kwang-Gill Lee, Young-H 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 2 105-109 (5 pages)
A histological examination at the postoperative 7th day revealed that the rhEGF treatment increased the number of proliferating nuclear antigen immunoreactive cells in the epidermis layer. In addition, the immunoreactive area of alpha-smooth muscle actin and the expression of prolyl 4-hydroxylase were significantly higher than those of the control group. Overall, a topical treatment of rhEGF ointment promotes wound healing by increasing the rate of epidermal proliferation and accelerating the... -
pH-dependent modulation of intracellular free magnesium ions with ion- selective electrodes in papillary muscle of guinea pig
Shang-Jin Kim, In-Gook Cho, Hyung-Sub Kang, Jin-Shang Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 5 31-36 (6 pages)
A change in pH can alter the intracellular concentration of electrolytes such as intracellular Ca2+ and Na+ ([Na+]i) that are important for the cardiac function.For the determination of the role of pH in the cardiac magnesium homeostasis, the intracellular Mg 2+ concentration ([Mg 2+]i), membrane potential and contraction in the papillary muscle of guinea pigs using ion-selective electrodes changing extracellular pH ([pH]o)or intracellular pH ([pH ]i )were measured in this study. A high... -
Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection
Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Hyoung Joon Moon, Tae Yung Kim, Bong Kyun 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 14 349-352 (4 pages)
(PEDV)enzyme-linked immunosorbent assay (ELISA) was compared with the serum neutralization (SN)test by testing 46 samples from experimentally infected sows,73 samples from naive sows,and 1,024 field sow samples from 48 commercial swine farms of undefined PED status. The SN test and the ELISA were performed using PEDV, KPEDV-9 strain.Viral proteins as a coating antigen of PEDV ELISA were extracted from the cytoplasm of PEDV-infected Vero cells using a non-ionic detergent, Triton X-100,and a... -
Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application
Deog Yong Lee, Young Wook Cho, Sang Gyun Kang, Sung Jae Shin, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 9 337-343 (7 pages)
by using Ni-NTA resin. Protein mass of 24 kDa was found with SDS-PAGE and the identity of the protein was confirmed by Western-blot. Production of polyclonal antibodies against rpIL-6 was performed using the purified rpIL-6 in mice and hens. An antigen capture-ELISA was developed with the antibodies after their extraction. To compare the IL-6 level in the different sanitary state of farms, pig sera were randomly collected and concentration of IL-6 in the sera was measured with the antigen... -
Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites
Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 8 139-145 (7 pages)
Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the N. caninum proteome. Protein sample preparation was carried out by first conducting sonication, followed by adding lysis buffer containing 7M urea plus 2M thiourea to the purified tachyzoites in order to complete disruption. A total of 335 differentially expressed... -
Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity
Sang-Hyun Kim, Yong-Hwan Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 5 119-124 (6 pages)
C. A few CR-binding negative (CR-) colonies occurred spontaneously within the population of CR+ isolate. The CR+ EHEC showed massive aggregative adhesion pattern, whereas the spontaneous CR- strain showed typical localized adherence on HEp-2 cells. Electron microscopy confirmed highly curliated bacteria in the CR+ EHEC sample. Interestingly, the curliation disappeared in a msbB1 and msbB2 double mutant derived from the CR+ EHEC. These results suggest that the compromised outer membrane integrity...


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