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121.

Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos

Kilyoung Song, Eunsong Lee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 12 81-87 (7 pages)

Kilyoung Song, Eunsong Lee 저
...
- 3,000원
maturation, embryo cleavage, or blastocyst formation after PA. β-ME treatment during IVM decreased embryo cleavage after SCNT compared to pFF or PVA treatments, but no significant difference was found in blastocyst formation (7-16%) among the four treatment groups. The results indicated that maturation of oocytes for 42 h was beneficial for the development of SCNT embryos. Furthermore, the defined maturation system used in this study could support in vitro development of PA or SCNT embryos.
122.

In vitro susceptibilities of Leptospira spp. and Borrelia burgdorferi isolates to amoxicillin, tilmicosin, and enrofloxacin

Doo Kim, Dorsey Kordick, Thomas Divers, Yung-Fu Chang 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 9 355-359 (5 pages)

Doo Kim, Dorsey Kordick, Thomas Divers, Yung-Fu Chang 저
...
- 1,500원
μg/ml, respectively. Two strains of B. burgdorferi were resistant to enrofloxacin at the highest concentration tested for MBC (≥100 μg/ml). Therefore, the potential role of tilmicosin in the treatment of leptospirosis and borreliosis should be further evaluated in animal models to understand whether the in vivo studies will confirm in vitro results. All spirochetal isolates were inhibited (MIC) and were killed (MBC) by tilmicosin at concentrations below the limit of testing (≤0.01 μg/ml).
123.

In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood

Ki-Soo Park, Yong-Soon Lee , Kyung-Sun Kang 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 7 343-348 (6 pages)

Ki-Soo Park, Yong-Soon Lee , Kyung-Sun Kang 저
...
- 3,000원
of MSCs from UCB still need to be established and evaluated. In this study, we isolated and characterized MSCs. UCB-derived mononuclear cells, which gave rise to adherent cells, exhibited either an osteoclast or a mesenchymal-like phenotype. The attached cells with mesenchymal phenotypes displayed fibroblast-like morphologies, and they expressed mesenchymal-related antigens (SH2 and vimentin) and periodic acid Schiff activity. Also, UCB-derived MSCs were able to transdifferentiate into bone and...
124.

Effect of β -mercaptoethanol or epidermal growth factor supplementation on in vitro maturation of canine oocytes collected from dogs with different stages of the estrus cycle

Min Kyu Kim, Yuda Heru Fibrianto, Hyun Ju Oh, Goo Jang, Hye Jin Kim, Kyu Seung Lee, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hwa 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 11 253-258 (6 pages)

Min Kyu Kim, Yuda Heru Fibrianto, Hyun Ju Oh, Goo Jang, Hye Jin Kim, Kyu Seung Lee, Sung Keun Kang, Byeong Chun Lee, Woo Suk Hwa 저
...
- 3,000원
of human oocyte and embryo development after in vitro fertilization (IVF). The effect of these two compounds were suggested to be mediated through the synthesis of glutathione (GSH) which is known to play an important role in protecting the cell or embryos from oxidative damage. Thus, it is suggested that supplementation of canine IVM medium with β-ME or EGF may be of benefit due to its positive role in IVM of various mammalian oocytes and embryo development, including cattle, pigs, rodents and...
125.

Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells

Jin-Hyuk Choi, Jun-Sung Kim, Young-Chul Kim, Yoon-Shin Kim, Nam-Hyun Chung and Myung-Haing Cho 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 3 11-18 (8 pages)

Jin-Hyuk Choi, Jun-Sung Kim, Young-Chul Kim, Yoon-Shin Kim, Nam-Hyun Chung and Myung-Haing Cho 저
...
- 3,000원
of larger size. This study was performed to evaluate PM2.5-induced oxidative stress in rat lung epithelial cell in order to provide basic data for the risk assessment of PM2.5. PM2.5 showed higher cytotoxicity than PM10. Also, PM 2.5 induced more malondialdehyde (MDA) formation than PM10. In Hoechst 33258 dye staining and DNA fragmentation assay, apopotic changes were clearly detected in PM2.5 treated cells in compared to PM10. Expression of catalase mRNA was increased by PM2.5 rather than PM10....
126.

Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System

Kwang-taek Lim, Byeong-chun Lee, Sung-keun Kang, Woo-suk Hwang 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 11 73-78 (6 pages)

Kwang-taek Lim, Byeong-chun Lee, Sung-keun Kang, Woo-suk Hwang 저
...
- 3,000원
In this study, we examined the effects of a two-step culture system, which involves the use of different culture media for early cleavage and later stage embryos, on the in vitro development of bovine embryos. We also investigated the effect of glucose, phosphate and citrate on the in vitro early developmental period of bovine embryos in a two-step culture system. Moreover, the supplementation of different protein sources (BSA-V, BSA-FAF and FBS) during IVC did not affect the frequency of...
127.

Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes

Kazim R. Chohan, Alan G. Hunter 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 10 67-72 (6 pages)

Kazim R. Chohan, Alan G. Hunter 저
...
- 3,000원
The objectives of the present study were to compare the in vitro maturation (IVM), fertilization and early embryonic development of bovine oocytes recovered from ovaries during the follicular, metestrus and diestrus stages of the estrous cycle and at anestrus and pregnancy after maturation in a serum free culture medium. Cumulus oocyte complexes (COCs) collected from ovaries at different reproductive statuses were matured in medium 199 supplemented with 10 g/ml FSH, 10 g/ml LH, 1.5 g/m...
128.

Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages

In-Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Choand Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 6 97-102 (6 pages)

In-Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Choand Han-Sang Yoo 저
...
- 3,000원
levels of IL-6, GM-CSF, and IL-12 in LPS-stimulated cells were almost always higher than those in control cells. Among those cytokines, IL-6 exhibited the predominant expression, and GM-CSF, IL-12 p40, and IL-12 p35 followed in the descending order. The times to reach the peak expression levels for IL-6, and GM-CSF, IL-12 p35, and IL-12 p40 were 14, and 24 h, respectively. After reaching the peak expression point, the expression levels of IL-6, GM-CSF, and IL-12 p40 reduced to the baseline by 72...
129.

Immunohistochemical detection of Prion protein (PrP-Sc) and epidemiological study of BSE in Korea

Hye Cheong Koo, Yong Ho Park, Byeong-Chun Lee, Chanhee Chae, Katherine I. O'Rourke, Timothy V. Baszler 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 4 25-31 (7 pages)

Hye Cheong Koo, Yong Ho Park, Byeong-Chun Lee, Chanhee Chae, Katherine I. O'Rourke, Timothy V. Baszler 저
...
- 3,000원
animals with TSEs. In this study, clinical and epidemiological examinations of bovine spongiform encephalopathy (BSE) were conducted in Korea. During the investigated period, none of the cattle exhibited typical clinical signs of BSE, such as behavioral disturbances, high sensitivity, and abnormal locomotion. Immunohistochemical analysis and western immunoblotting were established to detect PrP-Sc in the brain tissue using monoclonal antibody (MAb) F89/160.1.5, produced by immunizing mice with a...
130.

Immunohistochemical study of constitutive neuronal and inducible nitricoxide synthase in the central nervous system of goat with natural listeriosis

Taekyun Shin, Daniel Weinstock, Marlene D. Castro, Helene Acland, Mark Walter, Hyun Young Kim, H. Graham Purchase 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 3 77-80 (4 pages)

Taekyun Shin, Daniel Weinstock, Marlene D. Castro, Helene Acland, Mark Walter, Hyun Young Kim, H. Graham Purchase 저
...
- 1,500원
nNOS was weakly visualized in macrophages and neutrophils, while iNOS was expressed in macrophages, but not in neutrophils. These findings suggest that normal caprine brain cells, including neurons, constitutively express iNOS and nNOS, and the expressions of these molecules is increased in Listeria monocytogenes infections. Furthermore, inflammatory cells, including macrophages, expressing both nNOS and iNOS may play important roles in the pathogenesis of bacterial meningoencephalitis in goat.

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