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131.

Production of a highly group-specific monoclonal antibody against zearalenone and its application in an enzyme-linked immunosorbent assay

Sang-Ho Cha, Sung-Hee Kim, Karyn Bischoff, Hyun-Jeong Kim, Seong-Wan Son, Hwan-Goo Kang* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 2 119-125 (7 pages)

Sang-Ho Cha, Sung-Hee Kim, Karyn Bischoff, Hyun-Jeong Kim, Seong-Wan Son, Hwan-Goo Kang* 저
...
- 3,000원
A monoclonal antibody (mAb) against zearalenone (ZEN) was produced using ZEN-carboxymethoxylamine and -BSA conjugates. Antibody produced by one clone showing a very high binding ability was selected and found to have a higher affinity for ZEN compared to a commerciall ZEN antibody. We developed two direct competitive ELISA systems using the selected antibody (ZEN-coated and anti-ZEN antibody-coated ELISA). Quantitative ranges for the anti-ZEN antibody-coated ELISA and ZEN-coated ELISA were from...
132.

Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis

Siju Joseph*, Naicy Thomas, E. Thangapandian, Vijendra P Singh, Rishendra Verma, S. K. Srivastava 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 14 99-101 (3 pages)

Siju Joseph*, Naicy Thomas, E. Thangapandian, Vijendra P Singh, Rishendra Verma, S. K. Srivastava 저
...
- 1,500원
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based...
133.

Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate

Min-Kyoung Shin, Myung Hwan Jung, Won-Jung Lee, Pil Son Choi, Yong-Suk Jang, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 14 401-403 (3 pages)

Min-Kyoung Shin, Myung Hwan Jung, Won-Jung Lee, Pil Son Choi, Yong-Suk Jang, Han Sang Yoo* 저
...
- 1,500원
virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection.
134.

Biomarkers for identifying the early phases of osteoarthritis secondary to medial patellar luxation in dogs

Md. Rafiqul Alam*, Joong Ryong Ji, Min Su Kim, Nam Soo Kim 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 11 273-280 (8 pages)

Md. Rafiqul Alam*, Joong Ryong Ji, Min Su Kim, Nam Soo Kim 저
...
- 3,000원
The levels of tartrate resistant acid phosphatase (TRAP), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in synovial fluid (SF) and serum in cases of canine osteoarthritis (OA) were measured. OA was induced by a surgically-created medial patellar luxation in the left stifle of 24 dogs. SF and blood samples were collected at 1.5- and 3-month intervals, respectively. Every 3 months, one dog was euthanatized to collect tissue samples from both...
135.

Dynamic analysis of Ca level during bovine oocytes maturation and early embryonic development

Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 5 133-142 (10 pages)

Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 저
...
- 3,000원
cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca in the SCNT embryos. However, Ca was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca location and distribution changes during oocyte maturation and early embryo development processes should facilitate...
136.

In vitro toxicity of melamine against Tetrahymena pyriformis cells

Zhengfang Wang, Liben Chen, Rashad Al-Kasir, Bo Han* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 5 27-34 (8 pages)

Zhengfang Wang, Liben Chen, Rashad Al-Kasir, Bo Han* 저
...
- 3,000원
expression levels of T. pyriformis were examined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The results showed an obvious inhibitory effect of melamine on the growth of eukaryotic cells. Cell growth dynamics indicated that the IC50 value of melamine on T. pyriformis was 0.82 mg/mL. The cellular morphology was also affected in a concentration-dependent manner, with characteristics of atrophy or cell damage developing in the presence of...
137.

Antimutagenic and anticarcinogenic effect of methanol extracts of Petasites japonicus Maxim leaves

Hwan-Goo Kang, Sang-Hee Jeong*, †, Joon-Hyoung Cho 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 8 51-58 (8 pages)

Hwan-Goo Kang, Sang-Hee Jeong*, †, Joon-Hyoung Cho 저
...
- 3,000원
effect with the SOS chromotest and reverse mutation assay. The (anti-)carcinogenic effects were evaluated by the cytotoxicity on human cancer line cells and by the function and the expression of gap junctions in rat liver epithelial cell. PJ extracts significantly decreased spontaneous β-galactosidase activity and β-galactosidase activity induced by a mutagen, ICR, in Salmonella (S.) typhimurium TA 1535/pSK 1002. All doses of the extract (0.08-100 mg/plate) decreased the reversion frequency...
138.

Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

Seong In Lim, Wooseog Jeong, Dong Seob Tark, Dong Kun Yang, Chang Hee Kweon* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 9 331-336 (6 pages)

Seong In Lim, Wooseog Jeong, Dong Seob Tark, Dong Kun Yang, Chang Hee Kweon* 저
...
- 3,000원
Bovine leukemia virus (BLV) envelope glycoprotein (gp51/ gp30T-), consisting of BLV gp51 and BLV gp30 that lacked its C-terminal transmembrane domain, was expressed in insect cells under the control of the baculovirus polyhedron promoter. Recombinant BLV gp51/gp30T- secreted from insect cells was determined by immunofluorescence, enzyme- linked immunosorbent and western blot assays using a BLV-specific monoclonal antibody and BLV-positive bovine antibodies. An agar gel immunodiffusion (AGID)...
139.

Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand

Tawatchai Pohuang, Niwat Chansiripornchai*, Achara Tawatsin, Jiroj Sasipreeyajan 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 7 219-223 (5 pages)

Tawatchai Pohuang, Niwat Chansiripornchai*, Achara Tawatsin, Jiroj Sasipreeyajan 저
...
- 1,500원
Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and...
140.

Protective efficacy of commercial inactivated Newcastle disease virus vaccines in chickens against a recent Korean epizootic strain

Woo-Jin Jeon, Eun-Kyoung Lee, Young-Jeong Lee, Ok-Mi Jeong, Yong-Joo Kim, Jun-Hun Kwon, Kang-Seuk Choi 대한수의학회 Journal of Veterinary Science

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 10 295-294 (0 pages)

Woo-Jin Jeon, Eun-Kyoung Lee, Young-Jeong Lee, Ok-Mi Jeong, Yong-Joo Kim, Jun-Hun Kwon, Kang-Seuk Choi 저
...
- 무료
Despite the intensive vaccination policy that has been put in place to control Newcastle disease virus (NDV), the recent emergence of NDV genotype VII strains in Korea has led to significant economic losses in the poultry industry. We assessed the ability of inactivated, oil-emulsion vaccines derived from La Sota or Ulster 2C NDV strains to protect chickens from challenge with Kr-005/00, which is a recently isolated Korean epizootic genotype VII strain. Six-week-old SPF chickens were vaccinated...

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