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1491.

Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System

Hyun Soo Kim, Kwang Soon Shin, In Wook Hwang, Chul Joong Kim, Su Mi Kim, Hae Sun Jung 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 3 13-18 (6 pages)

Hyun Soo Kim, Kwang Soon Shin, In Wook Hwang, Chul Joong Kim, Su Mi Kim, Hae Sun Jung 저
...
- 3,000원
form of GP5 in mammalian cells, we have cloned the ORF5 gene from PRRSV CNV-1 into the Semliki Forest virus (SFV)-based expression vector, resulting in recombinant pSFV-ORF5. By the infection with recombinant pSFV-ORF5 to BHK-21 cells, the GP5 expression was confirmed by immunocytochemistry and immunoblotting assay. The recombinant virus particle harboring ORF5 gene was infectious to BHK-21 and MARC-145. The RNA synthesis and expression of GP5 in the infected cell was also confirmed by RT-PCR.
1492.

Cloning a new allele form of bovine TNF-a

Jongsam Ahn 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 12 139-141 (3 pages)

Jongsam Ahn 저
...
- 1,500원
T lymphocytes are early responders and modulators of immune responses against pathogens and cytokines such as IL-2, IL-7, IL-15 and TNF-a. To study the role TNF-a on gammadelta T lymphocytes, we cloned bovine TNF-a. Sequence analysis revealed that a new allele form of bovine TNF-a was cloned which has 3 additional nucleotide sequences as well as 3 nucleotide substitutions compared with previously reported bovine TNF-a. Further studies are needed to document the functional significance of a new...
1493.

Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos

Gook-jun Ahn, Byeong-chun Lee, Woo-suk Hwang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 11 131-137 (7 pages)

Gook-jun Ahn, Byeong-chun Lee, Woo-suk Hwang 저
...
- 3,000원
such as morula and blastocyst formation, the IP3 and ryanodine group exhibited significantly higher rates of morula fomation than the IP3 or control groups (40.6% vs 24.2% or 16.7%, respectively). Similarly, the rate of blastocyst formation in the IP3+ryanodine group was significantly higher than the control group (16.3% vs 6.9%) but did not differ significantly from the IP3 group (16.3% vs 9.5%). In nuclear transfer, activation was performed at 30 hpm by microinjection and elecroporation with...
1494.

Angiogenesis effects of nerve growth factor (NGF) on rat corneas

Kangmoon Seo, Jongil Choi, Myungjin Park, Changhun Rhee 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 10 125-130 (6 pages)

Kangmoon Seo, Jongil Choi, Myungjin Park, Changhun Rhee 저
...
- 3,000원
5.0 were higher than those of Groups 0 and 0.5 with the passage of time. The number, length, circumferential neovascularization and areas covered by the vessels in Groups 1.0 and 5.0 were significantly more than in Group 0 and Group 0.5 (p<0.05). This study showed that NGF had a dose-dependent angiogenic effects on the rat cornea and that the minimal effective dose of NGF was 1.0 ng per cornea. Also, it showed that NGF would be useful in angiogenic studies as an alternative angiogenic inducer.
1495.

Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6

Il-seob Shin, Hye-ryon Kim, Myung-jin Nam1 and Hwa-young Youn 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 8 115-120 (6 pages)

Il-seob Shin, Hye-ryon Kim, Myung-jin Nam1 and Hwa-young Youn 저
...
- 3,000원
This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and...
1496.

Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR

Yong-Hwan Kim, Kyu-Woan Cho, Hwa-Young Youn, Han Sang Yoo, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 10 59-63 (5 pages)

Yong-Hwan Kim, Kyu-Woan Cho, Hwa-Young Youn, Han Sang Yoo, Hong-Ryul Han 저
...
- 1,500원
up to increase efficiency in the diagnosis of canine distemper virus (CDV) infection after developement of nested PCR. Two PCR primer sets were designed based on the sequence of nucleocapsid gene of CDV Onderstepoort strain. One-step RT-PCR with the outer primer pair was revealed to detect 10(2) PFU/ml. The sensitivity was increased hundredfold using the one-step RT-PCR combined with the nested PCR. Specificity of the PCR was also confirmed using other related canine virus and peripheral blood...
1497.

Influence of gestational age at exposure on the prenatal effects of gamma-radiation

Sung-ho Kim, Se-ra Kim, Yun-sil Lee, Tae-hwan Kim, Sung-kee Jo, Cha-soo Lee 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 6 37-42 (6 pages)

Sung-ho Kim, Se-ra Kim, Yun-sil Lee, Tae-hwan Kim, Sung-kee Jo, Cha-soo Lee 저
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- 3,000원
18 of gestation and the fetuses were examined for mortality, growth retardation, change in head size and any other morphological abnormalities. The only demonstrable effect of irradiation during the preimplantation period was an increase in prenatal mortality. Resorptions were maximal on post-exposure day 2.5 after conception. The pre-implantation irradiated embryos which survived did not show any major fetal abnormalities. Small head, growth retardation, cleft palate, dilatation of the cerebral...
1498.

Capsular polysaccharide typing of domestic mastitis-causing Staphylococcus aureus strains and its potential exploration of bovine mastitis vaccine developmen. I. capsular olysaccharide typing, isolation and purification of the strains

Hong-Ryul Han, Son-Il Pak, Seung-Won Kang, Woo-Seog Jong, Cheol-Jong Youn 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 8 53-60 (8 pages)

Hong-Ryul Han, Son-Il Pak, Seung-Won Kang, Woo-Seog Jong, Cheol-Jong Youn 저
...
- 3,000원
strains. Some strains exhibited compact colonies in the SSA and expressed CP as determined by an enzyme-linked immunosorbent assay, indicating that compact morphology does not exclude encapsulation. The majority of the strains (11/12) showed autoagglutination in the salt aggregation test. The serotype 336 accounted for 46.7% of the isolates followed by serotype 5 (12.1%) and serotype 8 (12.1%). Particularly, twenty-six (24.3%) isolates reacted with two serotypes; 7 for type 8/336 and 19 for type...
1499.

Phenotypic and functional analysis of bovine gd lymphocytes

Yong Ho Park, Han Sang Yoo, Jang Won Yoon, Soo Jin Yang, Jong Sam An, W.C Davis 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 6 39-48 (10 pages)

Yong Ho Park, Han Sang Yoo, Jang Won Yoon, Soo Jin Yang, Jong Sam An, W.C Davis 저
...
- 3,000원
cell receptor (TCR). The study has also shown that the role of cytokines in activation and function of gammadelta T cells activated indirectly through cytokines secreted by ab T cells, accessory cells and antigen presenting cells (APC). Cytokines were differentially produced by subpopulations of gammadelta T cells under different conditions of activation. The investigation obtained in this study has revealed that factors account for activation and proliferation of gammadelta T cells in cultures...
1500.

Flow cytometric immunophenotyping of canine adipose-derived mesenchymal stem cells (ADMSCs) and feline ADMSCs using anti-human antibodies

Minho Ko , Kwon Young Lee , Sae Hoon Kim , Manho Kim , Jung Hoon Choi , Wooseok Im , Jin Young Chung 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 5 Pages

대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2018, 제 58권 제 1호 5 33-37 (5 pages)

Minho Ko , Kwon Young Lee , Sae Hoon Kim , Manho Kim , Jung Hoon Choi , Wooseok Im , Jin Young Chung 저
...
- 1,500원

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