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151.

Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis

Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)

Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 저
...
- 4,000원
that these factors are predominantly involved with binding and structural molecule activities. Vimentin and ㅁㅁα-enolase were central “functional hubs” in the network. Based on results from the present study, disease-induced alterations of protein expression in mammary glands and potential markers for the effective treatment of E. coli  mastitis were identified. These data have also helped elucidate defense mechanisms that protect the mammary glands and promote the pathogenesis of...
152.

Involvement of the Ca signaling pathway in osteoprotegerin inhibition of osteoclast differentiation and maturation

Yingxiao Fu, Jianhong Gu, Yi Wang, Yan Yuan, Xuezhong Liu, Jianchun Bian, Zong-Ping Liu 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 3 151-156 (6 pages)

Yingxiao Fu, Jianhong Gu, Yi Wang, Yan Yuan, Xuezhong Liu, Jianchun Bian, Zong-Ping Liu 저
...
- 3,000원
Ca/calmodulin-dependent protein kinase II (CaMKII) in the different treatment groups were measured by flow cytometry and Western blotting, respectively. The results confirmed that M-CSF + RANKL significantly increased [Ca]i and CaMKII phosphorylation in osteoclasts (p ＜ 0.01), and that these effects were subsequently decreased by OPG treatment. Exposure to specific inhibitors of the Ca signaling pathway revealed that these changes varied between the different OPG treatment groups. Findings from...
153.

Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus

Xiaoyu Deng, Yanlong Cong, Renfu Yin, Guilian Yang, Chan Ding, Shengqing Yu, Xiufan Liu, Chunfeng Wang, †, Zhuang Ding, 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 7 511-517 (7 pages)

Xiaoyu Deng, Yanlong Cong, Renfu Yin, Guilian Yang, Chan Ding, Shengqing Yu, Xiufan Liu, Chunfeng Wang, †, Zhuang Ding, 저
...
- 3,000원
Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two- dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78...
154.

Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos

Jongki Cho, Sungkeun Kang, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 8 225-231 (7 pages)

Jongki Cho, Sungkeun Kang, Byeong Chun Lee* 저
...
- 3,000원
and semi-quantified. More DNMT1 mRNA was detected in the transgenic SCNT group than the other three groups. Hsp 70.1 mRNA was detected in the IVF embryos, while lower levels were found in SCNT and parthenogenetic embryos. Mash2 mRNA was present at the highest levels in transgenic SCNT embryos. In conclusion, the higher levels of methylation and lower protein synthesis after heat shock in the transgenic SCNT embryos expected based on our results may cause lower embryonic development.
155.

Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus

Lishan Lv, Xiaoming Li, Genmei Liu, Ran Li, Qiliang Liu, Huifang Shen, Wei Wang, Chunyi Xue, Yongchang Cao* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 6 209-216 (8 pages)

Lishan Lv, Xiaoming Li, Genmei Liu, Ran Li, Qiliang Liu, Huifang Shen, Wei Wang, Chunyi Xue, Yongchang Cao* 저
...
- 3,000원
1 protein from avian influenza H5N1 virus and a fusion protein neuraminidase (NA)/spike 1 (S1) that was generated by fusing IBV S1 protein to the cytoplasmic and transmembrane domains of NA protein of avian influenza H5N1 virus. The chimeric VLPs elicited significantly higher S1-specific antibody responses in intramuscularly immunized mice and chickens than inactivated IBV viruses. Furthermore, the chimeric VLPs induced significantly higher neutralization antibody levels than inactivated H120...
156.

Mouse Fyn induces pseudopodium formation in Chinese hamster ovary cells

Lei An, Shengnan Liu, Wei Zhang, Yamei Zhang, Yingxue Huang, Xinde Hu, Shulin Chen, Shanting Zhao* 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 12 111-115 (5 pages)

Lei An, Shengnan Liu, Wei Zhang, Yamei Zhang, Yingxue Huang, Xinde Hu, Shulin Chen, Shanting Zhao* 저
...
- 1,500원
Molecular mechanisms underlying the effects of Fyn on cell morphology, pseudopodium movement, and cell migration were investigated. The Fyn gene was subcloned into pEGFP-N1 to produce pEGFP-N1-Fyn. Chinese hamster ovary (CHO) cells were transfected with pEGFP-N1-Fyn. The expression of Fyn mRNA and proteins was monitored by reverse transcription-PCR and Western blotting. Additionally, transfected cells were stained with 4´,6-diamidino-2- phenylindole and a series of time-lapse images was taken....
157.

Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine

Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 11 99-109 (11 pages)

Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 저
...
- 4,000원
The purpose of this study was to investigate the effects of porcine interleukin (IL)-2 and IL-4 genes on enhancing the immunogenicity of a porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine in piglets. Eukaryotic expression plasmids pcDNA-ORF5, pcDNA-IL-2, and pcDNA-IL-4 were constructed and then expressed in Marc-145 cells. The effects of these genes were detected using an indirect immunofluorescent assay and reverse transcription polymerase chain reaction (RT-PCR)....
158.

Development and clinical evaluation of a rapid diagnostic kit for feline leukemia virus infection

Won-Shik Kim, Chom-Kyu Chong, Hak-Yong Kim, Gyu-Cheol Lee, Wooseog Jeong, Dong-Jun An, Hye-Young Jeoung, Jae-In Lee, Young-Ki Le 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 10 91-97 (7 pages)

Won-Shik Kim, Chom-Kyu Chong, Hak-Yong Kim, Gyu-Cheol Lee, Wooseog Jeong, Dong-Jun An, Hye-Young Jeoung, Jae-In Lee, Young-Ki Le 저
...
- 3,000원
Feline leukemia virus (FeLV) causes a range of neoplastic and degenerative diseases in cats. To obtain a more sensitive and convenient diagnosis of the disease, we prepared monoclonal antibodies specific for the FeLV p27 to develop a rapid diagnostic test with enhanced sensitivity and specificity. Among these antibodies, we identified two clones (hybridomas 8F8B5 and 8G7D1) that specifically bound to FeLV and were very suitable for a diagnostic kit. The affinity constants for 8F8B5 and 8G7D1...
159.

A simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos

Sang Kyu Park, Sangho Roh*, Jong-Im Park* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 8 73-80 (8 pages)

Sang Kyu Park, Sangho Roh*, Jong-Im Park* 저
...
- 3,000원
Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression levels of developmentally important genes that may affect embryonic development and pregnancy. In the present study, porcine oocytes were treated with demecolcine that facilitated enucleation with protruding genetic material. Enucleation and donor...
160.

An overview of calf diarrhea - infectious etiology, diagnosis, and intervention

Yong-il Cho, Kyoung-Jin Yoon* 대한수의학회 Journal of Veterinary Science 17 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 1 1-17 (17 pages)

Yong-il Cho, Kyoung-Jin Yoon* 저
...
- 4,600원
Calf diarrhea is a commonly reported disease in young animals, and still a major cause of productivity and economic loss to cattle producers worldwide. In the report of the 2007 National Animal Health Monitoring System for U.S. dairy, half of the deaths among unweaned calves was attributed to diarrhea. Multiple pathogens are known or postulated to cause or contribute to calf diarrhea development. Other factors including both the environment and management practices influence disease severity or...

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