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전체 선택해제
총 708건 검색
본문검색 결과만 보기
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Enhanced immune response with foot and mouth disease virus VP1 and interleukin-1 fusion genes
Jong Hyeon Park, Sun Jin Kim, Jae Ku Oem, Kwang Nyeong Lee, Yong Joo Kim, Soo Jeong Kye, Jee Yong Park, Yi Seok Joo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 8 257-262 (6 pages)
with 2A (P1-2A)/IL-1α under the control of the human cytomegalovirus (HCMV) immediateearly promoter and intron. After DNA transfection into MA104 (monkey kidney) cells, Western blotting and an immunofluorescence assay were used to confirm the expression of VP1 or P1-2A and IL-1α. Mice were inoculated with the encoding plasmids via the intradermal route, and the IgG1 and IgG2a levels were used to determine the immune responses. These results show that although the immunized groups did not carry... -
Characterization of Chinook head salmon embryo phenotypes of infectious salmon anemia virus by real-time RT-PCR
Khalid Munir 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 13 167-176 (10 pages)
a onetube SYBR Green real-time RT-PCR assay for the detection and quantitation of infectious salmon anemia virus (ISAV) in various biological samples. The twofold aim of the present study was to verify that the optimized SYBR Green real-time RT-PCR conditions could detect ISAV isolates of different geographic origins, and to analyze the growth patterns of the selected ISAV isolates in the Chinook head salmon embryo (CHSE) -214 cells by this assay to better characterize their CHSE-phenotypes. A... -
Passive immunization using purified IgYs against infectious bursal disease of chickens in Pakistan
Muhammad Wasif Malik, Najma Ayub, Irfan Zia Qureshi 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 7 43-46 (4 pages)
with enzyme linked immunosorbant assay and agar gel precipitation test.Antibody titers were significantly higher in yolk than serum.Eggs collected at 28 days post-vaccination had maximum antibody titers. Of treatment regimens, T 3 was found to be most effective for hyperimmunization.Lyophilized antibodies stored at 4 oC did not lose their activity till the end of experiment.IBD virus infected birds were injected with purified antibodies which induced 92%recovery as compared to control birds. -
Histopathological changes of testes and eyes by neutron irradiation with boron compounds in mice
Yeon-Joo Kim, Won-Ki Yoon, SI-Yun Ryu, Ki-Jung Chun, Hwa-Young Son, Sung-Whan Cho 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 3 19-23 (5 pages)
investigate the biological effects of boron neutron capture therapy (BNCT)on the testes and eyes in mice using HANARO Nuclear Reactor, Korea Atomic Energy Research Institute.BNCT relies on the high capacity of 10B in capturing thermal neutrons. Sodium borocaptate (BSH, 75 ppm, iv)and borono-phenylalanine (BPA, 750 ppm, ip)have been used as the boron delivery agents.Mice were irradiated with neutron(flux:1.036739E +09, Fluence 9.600200E+12)by lying flatpose for 30 (10 Gy)or 100 min (33 Gy)with or... -
BSE situation and establishment of Food Safety Commission in Japan
Takashi Onodera, Chi-Kyeong Kim 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 1 1-11 (11 pages)
Eight major policies were implemented by Japanese Government since Oct.2001, to deal with bovine spongiform encephalopathy (BSE).These are;1)Surveillance in farm by veterinarian, 2)Prion test at healthy 1.3mi cows/yr, by veterinarian, 3)Elimination of specified risk material (SRM), 4)Ban of MBM for production, sale use, 5)Prion test for fallen stocks, 6)Transparent information and traceability, 7)New Measures such as Food Safety Basic Law, and 8)Establish of Food Safety Commission in the Cabinet... -
A predictive model for the level of sIgA based on IgG levels following the oral administration of antigens expressed in Sacchromyces cerevisiae
Sung Jae Shin, Seung Won Shin, Eun Jin Choi, Deog Yong Lee, Jeong Min Ahn, Moon Sik Yang, Yong Suk Jang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 8 305-309 (5 pages)
cerevisiae expressing the apxIA and apxIIA genes of A. pleuropneumoniae in this study. The correlation coefficient of determination (r2 x 100) for paired samples in both vaccinated and control groups showed a significant positive-relationship between IgG in sera and sIgA in the lung or intestine. These results indicated that IgG antibody titers in sera could be useful to indirectly predict local immune response, and sIgA, in the lung or intestine to evaluate the efficacy of an oral vaccination. -
Polysaccharides isolated from Phellinus gilvus enhances dermal wound healing in streptozotocin-induced diabetic rats
Jae-Sung Bae, Kwang-Ho Jang, Hee-Kyung Jin 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 12 161-164 (4 pages)
with biopsy punch on the 4th day after induction of diabetes. After 24 hours, each test substance was applied to the wound twice a day for next 5 days. Circular wounds treated with PG showed significantly reduced wound contraction and complete reepithelialization, as compared to wounds of non-treated (p < 0.05). These results show that polysaccharides isolated from PG enhanced wound repair in diabetic impaired healing, and could be developed as a wound healing agent in such clinical settings. -
Experimental evaluation of pathogenicity of Lactococcus garvieae in black rockfish (Sebastes schlegeli)
Sung-hyun Kang, Gee-wook Shin, Yong-seung Shin, Palaksha K. J., Young-rim Kim, Hyang-hee Yang, Eun-young Lee, Eung-goo Lee, Nam- 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 15 387-390 (4 pages)
due to bacterial diseases, particularly streptococcosis caused by Lactococcus garvieae. The bacterial surface characteristics of SJ7 and TY6 were found to have capsule but not NB13 and YS18. The experiential evaluation of L. garvieae pathogenicity, the capsular isolates showed high cumulative mortality i.e. SJ7 (100%) and TY6 (60%) compared to non-capsular isolates. Based on this result the capsular isolates L. garvieae were highly suspected as the causative agent of streptococcosis in rockfish. -
Molecular analysis of hprt mutation in B6C3F1 mice exposed to ozone alone and combined treatment of 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone and/or dibutyl phthalate for 32 and 52 weeks
Min Young Kim, Hyun Woo Kim, Jin Hong Park, Jun Sung Kim, Hwa Jin, Seo Hyun Moon, Kook Jong Eu, Hyun Sun Cho, Gami Kang, Yoon Sh 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 14 379-385 (7 pages)
h per day for 32- and 52-wk showed increases in the frequencies of TG rlymphocytes compared to the control groups. Additive interactions were noted from two combination groups compared to the ozone alone in both sexes of 32- and 52-wk studies. The most common specific mutation type in the hprt genes of test materials-treated male and female mice was transversion with very few transition. The results indicate that such dominant transversion may be responsible for toxicity and combined exposure to... -
Cap-independent protein translation is initially responsible for 4-(N-methylnitrosamino)-1-(3-pyridyl)-butanone (NNK)-induced apoptosis in normal human bronchial pithelial cells
Seo-Hyun Moon, Hyun-Woo Kim, Jun-Sung Kim, Jin-Hong Park, Hwa Kim, Gook-Jong Eu, Hyun-Sun Cho, Ga-Mi Kang, Kee-Ho Lee, Myung-Hai 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 13 369-378 (10 pages)
Evidences show that eukaryotic mRNAs can perform protein translation through internal ribosome entry sites (IRES). 5'-Untranslated region of the mRNA encoding apoptotic protease-activating factor 1 (Apaf-1) contains IRES, and, thus, can be translated in a cap-independent manner. Effects of changes in protein translation pattern through rapamycin pretreatment on 4-(methylnitrosamino)-1-(3-pyridyl)-butanone(NNK, tobacco-specific lung carcinogen)-induced apoptosis in human bronchial epithelial...
