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본문검색 결과만 보기
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Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies
Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 6 41-46 (6 pages)
Five monoclonal antibodies (MAbs) and chicken immunoglobulin (IgY) were developed by immunizing with flagella purified from Listeria monocytogenes 4b and the five MAbs have been confirmed to be specific against three different epitopes of flagellin. The antibodies showed specific reaction to Listeria genus and no cross-reactivity with other bacteria tested in this experiment including E.coli O157:H7 and Salmonella enteritidis. Sandwich enzyme-linked immunosorbent assays (ELISA) using the MAbs... -
Induction of castration by immunization of male dogs with recombinant gonadotropin-releasing hormone (GnRH)-canine distemper virus (CDV) T helper cell epitope p35
Mi-Jeong Jung, Young-Chan Moon, Ik-Hyun Cho, Jung-Yong Yeh, Sun-Eui Kim, Wha-Seok Chang, Seung-Young Park, Chang-Seon Song, Hwi- 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 3 21-24 (4 pages)
Immunocastration is a considerable alternative to a surgical castration method especially in male animal species for alleviating unwanted male behaviors and characteristics. Induction of high titer of antibody specific for gonadotropin-releasing hormone (GnRH) correlates with the regression of testes. Fusion proteins composed of canine GnRH and T helper (Th) cell epitope p35 originated from canine distemper virus (CDV) F protein and goat rotavirus VP6 protein were produced in E. coli. When these... -
Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD
Ji-Yeon Kim, So-Hyun Kim, Nam-Hoon Kwon, Won-Ki Bae, Ji-Youn Lim, Hye-Cheong Koo, Jun-Man Kim, Kyoung-Min Noh, Woo-Kyung Jung, K 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 2 7-19 (13 pages)
Escherichia coli O157:H7 is recognized as a significant food-borne pathogen, so rapid identification is important for food hygiene management and prompt epidemiological investigations. The limited prevalence data on Shiga toxin-producing E. coli (STEC) and E. coli O157:H7 in foods and animals in Korea made an assessment of the risks difficult, and the options for management and control unclear. The prevalence of the organisms was examined by newly developed kit-E. coli O157:H7 Rapid kit. For the... -
Effect of soluble porcine aminopeptidase N on antibody production against porcine epidemic diarrhea virus
Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Han Sang Yoo, Yong Suk Jang, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 11 353-357 (5 pages)
A few members of coronavirus group I which includes porcine epidemic diarrhea virus (PEDV) use porcine aminopeptidase N (pAPN) as a cellular receptor. Cellular receptors play an important role in virus attachment and entry. However, the low permissiveness of PEDV to APN-expressing porcine cell lines has made it difficult to elucidate the role of pAPN in vitro. The purpose of this study was to prove whether the treatment of soluble pAPN could enhance the antibody production against PEDV in guinea... -
Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application
Deog Yong Lee, Young Wook Cho, Sang Gyun Kang, Sung Jae Shin, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 9 337-343 (7 pages)
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and accurate quantification method previously used with ovine, rat, and human IL-6 proteins. However, it has never been reported to quantify porcine IL-6 protein using capture ELISA. In this study, we generated and characterized a set of IgY and mono-specific polyclonal antibodies to recombinant porcine IL-6 (rpIL-6),... -
Electroacupuncture and Moxibustion for Correction of Abomasal Displacement in Dairy Cattle
Kwang-ho Jang, Joo-myoung Lee, Tchi-chou Nam 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 14 93-95 (3 pages)
This study was performed to investigate the therapeutic effect of electroacupuncture and moxibustion on abomasal displacement in dairy cattle.After acupuncture needles were inserted bilaterally into the acupoints, 'Pi yu', 'Wei yu' and 'Guan yuan yu' , electronic stimulation (5 Hz and 10 V, 20 minutes) was conducted once a day for 3 days consecutively. Modified moxa patch was also applied at the same acupoints as in acupuncture for 3 days consecutively.Ten among twelve cows with abomasal... -
Characterization of Antigenic Sites on the Rinderpest Virus N Protein Using Monoclonal Antibodies
Kang-seuk Choi, Jin-ju Nah, Young-joon Ko, Cheong-up Choi, Jae-hong Kim, Shien-young Kang, Yi-seok Joo 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 9 57-65 (9 pages)
The N protein of the rinderpest virus (RPV) was analyzed topologically and antigenically by using anti-N monoclonal antibodies (Mabs). Ten Mabs were raised against the N protein of the RPV. At least six non-overlapping antigenic sites (sites A-F) were delineated by competitive binding assays using biotinylated Mabs. Of them 5 sites (A, C, D, E and F) on the N protein were recognized by RPV-specific Mabs in ELISA and IFA while site B was recognized by Mabs reacting with both RPV and PPRV. Non-... -
Topology of Scavenger Receptor Class B Type I (SR-BI) on Brush Border Membrane
Chang-Hoon Han and Mun-Han Lee 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 3 265-272 (8 pages)
Both hydropathy plot and in vitro translation results predict the topology of SR-BI; the receptor is an integral membrane protein of 509 amino acids, consisting of a short cytoplasmic N-terminus of 9 amino acids followed by a first transmembrane domain of 22 amino acids, the extracellular domain of 408 amino acids, the second transmembrane domain of 22 amino acids, and the cytoplasmic C-terminus of 47 amino acids. The immunoblot of rBBMV in the presence or absence of pAb589 peptide antigen (the... -
Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays
In-Soo Choi and Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 10 207-212 (6 pages)
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by... -
Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica , Pasteurella multocida and Actinobacillus pleuropneumoniae
Jong Man Kim, Han Sang Yoo, In Soo Choi, Won Hur, Na Ri Shin 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 8 47-57 (11 pages)
Swine respiratory diseases induce severe economic losses in the swine industry worldwide. Several methods have been developed and applied to control these diseases. However, there are still problems of disease control in the swine industry. Recently, egg yolk antibodies have been found to offer several advantages for disease control in animals and humans. In a previous study (24), antibodies to several causative pathogens of swine respiratory diseases were developed. However, several problems...
