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161.

Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model

Dong-Hun Lee, Jong-In Kim, Jae-Won Lee, Wook-Hun Chung, Jae-Keun Park, Yu-Na Lee, Jin Soo Han, Hwi-Yool Kim, Sang-Won Lee, Chang 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 14 439-442 (4 pages)

Dong-Hun Lee, Jong-In Kim, Jae-Won Lee, Wook-Hun Chung, Jae-Keun Park, Yu-Na Lee, Jin Soo Han, Hwi-Yool Kim, Sang-Won Lee, Chang 저
...
- 1,500원
The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows...
162.

Effects of progestagen exposure duration on estrus synchronization and conception rates of crossbreed ewes undergoing fixed time artificial insemination

Wanessa Blaschi, Paula A. Lunardelli, Luciana S.R. Marinho, Marilu C. Max, Gustavo M.G. Santos, Katia C. Silva-Santos, Fabiana A 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 13 433-437 (5 pages)

Wanessa Blaschi, Paula A. Lunardelli, Luciana S.R. Marinho, Marilu C. Max, Gustavo M.G. Santos, Katia C. Silva-Santos, Fabiana A 저
...
- 1,500원
Synchronization of estrus and ovulation are of paramount importance in modern livestock improvement programs. These methods are critical for assisted reproduction technologies, including artificial insemination and embryo transfer, that can increase productivity. In the current study, subcutaneous implants containing norgestomet were placed for long (14 days), medium (9 days), and short (5 days) periods of time in 70 crossbred ewes undergoing fixed-time artificial insemination. The resulting...
163.

Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides

Hong-tao Chang, Xiu-yuan He, Yu-Feng Liu, Lu Chen*, Quan-hai Guo, Qiu-ying Yu, Jun Zhao, Xin-wei Wang, Xia Yang, Chuan-qing Wang 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 8 399-407 (9 pages)

Hong-tao Chang, Xiu-yuan He, Yu-Feng Liu, Lu Chen*, Quan-hai Guo, Qiu-ying Yu, Jun Zhao, Xin-wei Wang, Xia Yang, Chuan-qing Wang 저
...
- 3,000원
A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva,...
164.

Molecular characterization of duck enteritis virus CHv strain UL49.5 protein and its colocalization with glycoprotein M

Meng Lin, Renyong Jia*, Mingshu Wang, Xinghong Gao, Dekang Zhu, Shun Chen, Mafeng Liu, Zhongqiong Yin, Yin Wang, Xiaoyue Chen, A 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 7 389-398 (10 pages)

Meng Lin, Renyong Jia*, Mingshu Wang, Xinghong Gao, Dekang Zhu, Shun Chen, Mafeng Liu, Zhongqiong Yin, Yin Wang, Xiaoyue Chen, A 저
...
- 3,000원
The UL49.5 gene of most herpesviruses is conserved and encodes glycoprotein N. However, the UL49.5 protein of duck enteritis virus (DEV) (pUL49.5) has not been reported. In the current study, the DEV pUL49.5 gene was first subjected to molecular characterization. To verify the predicted intracellular localization of gene expression, the recombinant plasmid pEGFP-C1/pUL49.5 was constructed and used to transfect duck embryo fibroblasts. Next, the recombinant plasmid pDsRed1-N1/ glycoprotein M (gM)...
165.

Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) from cattle in Korea between 2010 and 2011

Eun Kang, Sun Young Hwang, Ka Hee Kwon, Ki Yeon Kim, Jae Hong Kim, Yong Ho Park* 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 5 369-379 (11 pages)

Eun Kang, Sun Young Hwang, Ka Hee Kwon, Ki Yeon Kim, Jae Hong Kim, Yong Ho Park* 저
...
- 4,000원
A total of 156 Shiga-like toxin producing Escherichia coli (STEC) were isolated from fecal samples of Korean native (100/568, 18%) and Holstein dairy cattle (56/524, 11%) in Korea between September 2010 and July 2011. Fifty-two STEC isolates (33%) harbored both of shiga toxin1 (stx1) and shiga toxin2 (stx2) genes encoding enterohemolysin (EhxA) and autoagglutinating adhesion (Saa) were detected by PCR in 83 (53%) and 65 (42%) isolates, respectively. By serotyping, six STEC from native cattle and...
166.

Distribution, quantitative load and characterization of Salmonella associated with swine farms in upper-northern Thailand

Pakpoom Tadee, Kittipong Kumpapong, Danai Sinthuya, Panuwat Yamsakul, Nipa Chokesajjawatee, Supachai Nuanualsuwan, Suchawan Porn 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 20 327-334 (8 pages)

Pakpoom Tadee, Kittipong Kumpapong, Danai Sinthuya, Panuwat Yamsakul, Nipa Chokesajjawatee, Supachai Nuanualsuwan, Suchawan Porn 저
...
- 3,000원
This study was conducted to analyze the prevalence and quantitative loads of Salmonella spp. on pig farms in Chiang Mai, Lamphun, Thailand to assess loading levels before slaughtering. The serotype diversity, antimicrobial-resistance pattern and pulse-field type of Salmonella spp. were also characterized to assess the dynamic propagation of the pathogen. The Salmonella-positive prevalence was 246/805 (30.56%), and the quantitative loads varied from 1.48∼4.04 Log10MPN/g, with a mean ± standard...
167.

Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 19 317-325 (9 pages)

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 저
...
- 3,000원
A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for simultaneous detection of the stx1 and stx2 genes and applied for detection of shiga toxin-producing Escherichia coli (STEC) in cattle farm samples. Two target genes were distinguished based on Tm values of 85.03 ± 0.54C for stx1 and 87.47 ± 0.35C for stx2. The mLAMP assay was specific (100% inclusivity and exclusivity), sensitive (with a detection limit as low as 10 fg/μL), and quantifiable (R = 0.9313). The...
168.

Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber

Isabelle Ruhnke*, John V. DeBiasio, Jan S. Suchodolski, Shelley Newman, Mark W. Musch, Jörg M. Steiner 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 17 297-307 (11 pages)

Isabelle Ruhnke*, John V. DeBiasio, Jan S. Suchodolski, Shelley Newman, Mark W. Musch, Jörg M. Steiner 저
...
- 4,000원
This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from...
169.

Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model

Jong Min Kim, Myoung Hwan Kim, Seong Soo Kang, Gonhyung Kim, Seok Hwa Choi* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 16 289-295 (7 pages)

Jong Min Kim, Myoung Hwan Kim, Seong Soo Kang, Gonhyung Kim, Seok Hwa Choi* 저
...
- 3,000원
We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model. Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based...
170.

Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice

Haiyan Zhao, Yalan Wang, Zhitao Ma, Yongqiang Wang, Wen-hai Feng* 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 5 199-208 (10 pages)

Haiyan Zhao, Yalan Wang, Zhitao Ma, Yongqiang Wang, Wen-hai Feng* 저
...
- 3,000원
to this construct were analyzed in mice. Intestinal mucosal PRRSV-specific sIgA antibody and higher levels of IFN-γ in spleen CD4 and CD8 T cells were induced by oral administration of Yeast-GP5. Additionally, Yeast-GP5 administered subcutaneously evoked vigorous cell-mediated immunity, and PRRSV-specific lymphocyte proliferation and IFN-γ secretion were detected in the splenocytes of mice. These results suggest that Yeast-GP5 has the potential for use as a vaccine for PRRSV in the future.

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