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Role of Dopamine Receptors on Electroencephalographic Changes Produced by Repetitive Apomorphine Treatments in Rats
HwanSooJang, JiYoungKim, SangHeonKim, Maan-GeeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.3 2 147-151 (5 pages)
Repeated psychostimulants induce electroencephalographic (EEG) changes, which reflect adaptation of the neural substrate related to dopaminergic pathways. To study the role of dopamine receptors in EEG changes, we examined the effect of apomorphine, the dopamine D1 receptor antagonist, SCH-23390, and the D2 receptor antagonist, haloperidol, on EEG in rats. For single and repeated apomorphine treatment groups, the rats received saline or apomorphine for 4 days followed by a 3-day withdrawal... -
Multiple Residues in the P-Region and M2 of Murine Kir 2.1 Regulate Blockage by External Ba2＋
YoungMeeLee, GarethA.Thompson, IanAshmole, MarkLeyl, InsukSo, PeterR.Stanfield 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.1 10 61-69 (9 pages)
We have examined the effects of certain mutations of the selectivity filter and of the membrane helix M2 on Ba2＋ blockage of the inward rectifier potassium channel, Kir 2.1. We expressed mutant and wild type murine Kir 2.1 in Chinese hamster ovary (CHO) cells and used the whole cell patch-clamp technique to record K＋ currents in the absence and presence of externally applied Ba2＋. Wild type Kir2.1 was blocked by externally applied Ba2＋ in a voltage and concentration dependent manner. Mutants... -
Alterations in Membrane Transport Function and Cell Viability Induced by ATP Depletion in Primary Cultured Rabbit Renal Proximal Tubular Cells
SungJuLee, ChaeHwaKwon, YongKeunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.1 3 15-22 (8 pages)
This study was undertaken to elucidate the underlying mechanisms of ATP depletion-induced membrane transport dysfunction and cell death in renal proximal tubular cells. ATP depletion was induced by incubating cells with 2.5 mM potassium cyanide (KCN)/0.1 mM iodoacetic acid (IAA), and membrane transport function and cell viability were evaluated by measuring Na＋-dependent phosphate uptake and trypan blue exclusion, respectively. ATP depletion resulted in a decrease in Na＋-dependent phosphate... -
Voltage-dependent Ca2＋ Current Identified in Freshly Isolated Interstitial Cells of Cajal (ICC) of Guinea-pig Stomach
YoungChulKim, HikaruSuzuki, Wen-XieXu, HikaruHashitani, WoongChoi, Hyo-YungYun, Seon-MeePark6, SeiJinYoun6, Sang-JeonLee, SangJinLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.6 6 323-330 (8 pages)
spontaneous inward current under K＋-rich pipette solution. The voltage clamp experiments revealed the amplitude of - 329 pA inward current at irregular frequency. With Cs＋-rich pipette solution at Vh=−80 mV, ICC-MY produced voltage-dependent inward currents (VDIC), and nifedipine (1ՌM) blocked VDIC. Therefore, we successfully isolated c-Kit positive single ICC from guinea-pig stomach, and found that ICC-MY potently produced dihydropiridine sensitive L-type voltage-dependent Ca2＋... -
Eugenol Inhibits ATP-induced P2X Currents in Trigeminal Ganglion Neurons
HaiYingLi, ByungKyLee, JoongSooKim, SungJunJung, SeogBaeOh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.6 5 315-322 (8 pages)
ATP-induced currents in both capsaicin-sensitive and capsaicin-insensitive TG neurons with similar extent, and most ATP-responsive neurons were IB4-positive. Eugenol inhibited not only Ca2＋ transients evoked by Ձ,Ղ-meATP, the selective P2X3 agonist, in capsaicin-insensitive TG neurons, but also ATP-induced currents in P2X3-expressing HEK293 cells without co-expression of transient receptor potential vanilloid 1 (TRPV1). We suggest, therefore, that eugenol inhibits P2X3 currents in a... -
Pre-ischemic Treatment with Ampicillin Reduces Neuronal Damage in the Mouse Hippocampus and Neostriatum after Transient Forebrain Ischemia
Kyung-EonLee, Seul-KiKim, Kyung-OkCho, SeongYunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.6 1 287-292 (6 pages)
Ampicillin, a Ղ-lactam antibiotic, has been reported to induce astrocytic glutamate transporter-1 which plays a crucial role in protecting neurons against glutamate excitotoxicity. We investigated the effect of ampicillin on neuronal damage in the mouse hippocampus and neostriatum following transient global forebrain ischemia. Male C57BL/6 mice were anesthetized with halothane and subjected to bilateral occlusion of the common carotid artery for 40 min. Ampicillin was administered... -
Acidic pH-activated Cl? Current and Intracellular Ca2＋ Response in Human Keratinocytes
SuJungPark, WonWooChoi, OhSangKwon, JinHoChung, HeeChulEun, YungEEarm, SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.4 8 177-184 (8 pages)
The layers of keratinocytes form an acid mantle on the surface of the skin. Herein, we investigated the effects of acidic pH on the membrane current and [Ca2＋]c of human primary keratinocytes from foreskins and human keratinocyte cell line (HaCaT). Acidic extracellular pH (pHe≤5.5) activated outwardly rectifying Cl− current (ICl,pH) with slow kinetics of voltage-dependent activation. ICl,pH was potently inhibited by an anion channel blocker 4,4`-diisothiocyanostilbene-2,2`-disulphonic... -
Resveratrol Inhibits Nicotinic Stimulation-Evoked Catecholamine Release from the Adrenal Medulla
Seong-ChangWoo, Gwang-MoonNa, Dong-YoonLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.4 5 155-164 (10 pages)
selective neuronal nicotinic Nn receptor agonist, 100ՌM) and McN-A-343 (a selective muscarinic M1 receptor agonist, 100ՌM) in both a time- and dose- dependent fashion. Also, in the presence of resveratrol (30ՌM), the secretory responses of CA evoked by veratridine 8644 (an activator of voltage-dependent Na＋ channels, 100ՌM), Bay-K-8644 (a L-type dihydropyridine Ca2＋ channel activator, 10ՌM), and cyclopiazonic acid (a cytoplasmic Ca2＋-ATPase inhibitor, 10ՌM)... -
Expression of Endothelin-1 and Its Receptors in Cisplatin-Induced Acute Renal Failure in Mice
SeokwooLee, DowhanAhn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.4 4 149-154 (6 pages)
Endothelin-1 (ET-1) is unequivocally elevated in the kidney with ischemic acute renal failure (ARF), whereas ET receptors (ETAR and ETBR) are variably expressed. Although renal functional and structural changes are similar between ischemic and nephrotoxic ARF, there are few reports on the alteration in the ET system in nephrotoxic ARF. This study was, therefore, undertaken to investigate changes in renal expression of ET-1 and its receptors in nephrotoxic ARF induced by cisplatin. Mice were... -
Influence of Ketamine on Catecholamine Secretion in the Perfused Rat Adrenal Medulla
Young-YeobKo, Yong-HoonJeong, Dong-YoonLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.3 3 101-110 (10 pages)
selective neuronal nicotinic NN receptor agonist, 100ՌM) and McN-A-343 (a selective muscarinic M1 receptor agonist, 100ՌM). Also, in the presence of ketamine (100ՌM), the CA secretory responses evoked by veratridine (a voltage-dependent Na＋ channel activator, 100ՌM), Bay-K-8644 (an L-type dihydropyridine Ca2＋ channel activator, 10ՌM), and cyclopiazonic acid (a cytoplasmic Ca2＋-ATPase inhibitor, 10ՌM) were significantly reduced, respectively. Interestingly,...
