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Detection of Clavibacter michiganensis subsp. michiganensis Assisted by Micro- Raman Spectroscopy under Laboratory Conditions
Moisé, s Roberto Vallejo Pé, rez, Hugo Ricardo Navarro Contreras, Jesú, s A. Sosa Herrera, José, Pablo Lara Á, vila 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 5호 4 381-392 (12 pages)
the distinguishable markers. Chemometric analyses showed the best performance by the implementation of PCA-LDA supervised classification algorithms applied over Raman spectrum data with 100% of performance in metrics of classifiers (sensitivity, specificity, accuracy, negative and positive predictive value) that allowed us to differentiate Cmm from other endophytic bacteria (Bacillus and Pantoea). The unsupervised KMeans algorithm showed good performance (100, 96, 98, 91 y 100%, respectively). -
Management of Citrus Canker in Argentina, a Success Story
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 5호 1 441-449 (9 pages)
Citrus canker is an important bacterial disease of citrus in several regions of the world. Strains of Xanthomonas citri type-A (Xc-A) group are the primary pathogen where citrus canker occurs. After Xc-A entered the Northeast of Argentina in 1974, the disease spread rapidly from 1977 to 1980 and then slowed down and remained moving at slow pace until 1990 when it became endemic. Citrus canker was detected in Northwest Argentina in 2002. This paper presents the main steps in the fight of the... -
Reaction of Cauliflower Genotypes to Black Rot of Crucifers
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 10 181-185 (5 pages)
This study aimed to evaluate six cauliflower genotypes regarding their resistance to black rot and their production performance. To do so, it was conducted two field experiments in Ipameri, Goiás, Brazil, in 2012 and 2013. It was used a randomized block design, with four replications (total of 24 plots). Each plot consisted of three planting lines 2.5 m long (six plants/line), spaced 1.0 m apart, for a total area of 7.5 m. Evaluations of black rot severity were performed at 45 days... -
Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 4 123-131 (9 pages)
Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced... -
Genetic Diversity and Differentiation of Colletotrichum spp. Isolates Associated with Leguminosae Using Multigene Loci, RAPD and ISSR
한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 1호 2 10-24 (15 pages)
Genetic diversity and differentiation of 50 Colletotrichum spp. isolates from legume crops studied through multigene loci, RAPD and ISSR analysis. DNA sequence comparisons by six genes (ITS, ACT, Tub2, CHS-1, GAPDH, and HIS3) verified species identity of C. truncatum, C. dematium and C. gloeosporiodes and identity C. capsici as a synonym of C. truncatum. Based on the matrix distance analysis of multigene sequences, the Colletotrichum species showed diverse degrees of intera and interspecific... -
Research Articles : Prevalence of Tobacco mosaic virus in Iran and Evolutionary Analyses of the Coat Protein Gene
한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 3호 5 260-273 (14 pages)
The incidence and distribution of Tobacco mosaic virus (TMV) and related tobamoviruses was determined using an enzyme-linked immunosorbent assay on 1,926 symptomatic horticultural crops and 107 asymptomatic weed samples collected from 78 highly infected fields in the major horticultural crop-producing areas in 17 provinces throughout Iran. The results were confirmed by host range studies and reverse transcription-polymerase chain reaction. The overall incidence of infection by these viruses in... -
A Forecast Model for the First Occurrence of Phytophthora Blight on Chili Pepper after Overwintering
한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 2호 6 172-184 (13 pages)
An infection risk model for Phytophthora blight on chili pepper was developed to estimate the first date of disease occurrence in the field. The model consisted of three parts including estimation of zoosporangium formation, soil water content, and amount of active inoculum in soil. Daily weather data on air temperature, relative humidity and rainfall, and the soil texture data of local areas were used to estimate infection risk level that was quantified as the accumulated amount of active... -
Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 5 128-137 (10 pages)
Fifty two Macrophomina phaseolina isolates were recovered from 24 host plant species through the 14 Iranian provinces. All isolates were confirmed to species using species-specific primers. The colony characteristics of each isolate were recorded, including chlorate phenotype, relative growth rate at 30 -
Reverse Transcription Droplet Digital PCR을 활용한 Tomato Spotted Wilt Virus 검출 및 정량
이효정, 박기범, 한연수, 정래동 한국식물병리학회 식물병연구 8 Pages
한국식물병리학회 식물병연구 2021, 27권 3호 5 120-127 (8 pages)
식물 바이러스는 작물 수확량에 상당한 손실을 일으키고 작 물 생산을 지속적으로 위협하여 세계 식량 안보에 심각한 위협 이 된다. 그 중 tomato spotted wilt virus (TSWV)는 주로 원예 작물을 감염시키는 가장 위협적인 식물 바이러스로 넓은 기주 범위를 가진다. Reverse-transcription quantitative real-time PCR (RT-qPCR)은 TSWV의 민감한 검출을 위해 널리 사용되 고 있지만 표준화의 어려움으로 인해 유용성이 감소한다. 따라 서 본 연구에서는 TSWV 검출을 위해 민감하고 정확한 reverse transcription droplet digital... -
당근검은잎마름병균 Alternaria dauci에 대한 살균제 효과 검정 및 병원 균 집단에 대한 저항성 검정
도지원, 민지영, 김용수, 박용, 김흥태 한국식물병리학회 식물병연구 11 Pages
한국식물병리학회 식물병연구 2020, 26권 2호 1 61-71 (11 pages)
당근검은잎마름병균인 Alternaria dauci KACC42997에 대해 서 32개 살균제를 선발하여 균사생장 억제효과를 조사한 결과, ‘다2‘군, ’다5‘군, ’사1‘군, ’마2‘군, ’마3‘군에 속하는 살균제의 균사 생장 억제효과가 우수하였다. Iminoctadine tris-albesilate를 제외한 ‘카’군에 속하는 보호살균제와 ’다3‘군에 속하는 pyra- clostrobin은 병원균의 포자발아를 억제하는 효과가 우수하였 다. 균사생장 억제효과가 우수했던 숙신산탈수소효소 활성을 저해하는 ’다2‘군과 탈메틸효소 활성을 저해하는 ’사1‘군 살균 제는...


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