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전체 선택해제
총 58건 검색
본문검색 결과만 보기
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Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips
Na-Kyeong Kim, Hyo-Jeong Lee, Sang-Min Kim, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 8 159-166 (8 pages)
RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specificity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to detect BYDV in oat samples from six different regions and in individual aphids. These results confirm the outstanding potential of the... -
Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid and Sensitive Detection of Barley Yellow Dwarf Virus in Oat
Na-Kyeong Kim, Sang-Min Kim, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 12 497-502 (6 pages)
important plant pathogen that causes stunted growth, delayed heading, leaf yellowing, and purple leaf tip, thereby reducing the yields of cereal crops worldwide. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed for the detection of BYDV in oat leaf samples. The RT-RPA assay involved incubation at an isothermal temperature (42°C) and could be performed rapidly in 5 min. In addition, no cross-reactivity was observed to occur with othe... -
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification
Nam-Yeon Kim, Jong-hee Oh, Su-Heon Lee, Hong-sup Kim, Jae Sun Moon, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 12 575-579 (5 pages)
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an... -
Diffusible and Volatile Antifungal Compounds Produced by an Antagonistic Bacillus velezensis G341 against Various Phytopathogenic Fungi
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2017, 33권 5호 6 488-498 (11 pages)
The aim of this study was to identify volatile and agar-diffusible antifungal metabolites produced by Bacillus sp. G341 with strong antifungal activity against various phytopathogenic fungi. Strain G341 isolated from four-year-old roots of Korean ginseng with rot symptoms was identified as Bacillus velezensis based on 16S rDNA and gyrA sequences. Strain G341 inhibited mycelial growth of all phytopathogenic fungi tested. In vivo experiment results revealed that n-butanol extract of fermentation... -
Control Efficacy of Phloretin Isolated from Apple Fruits Against Several Plant Diseases
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 13 280-285 (6 pages)
In the course of a searching natural antifungal compounds from plant sources, we found that the methanol extract (3,000 μg/ml) of Malus domestica fruits had potential of control against rice blast (Magnaporthe grisea) and tomato late blight (Phytophthora infestans). Under bioassay-guided purification, we isolated phloretin, a phenolic compound, with in vivo antifungal activity against M. grisea. By 1-day protective application of phloretin (500 μg/ml), the compound strongly inhibited the... -
Cnidium officinale, Fungal disease, Fusarium solani, Fusarium wilt
Na-Kyeong Kim, Hyo-Jeong Lee, Tae-Ho Ryu, In-Sook Cho, Ho-Jong Ju, Rae-Dong Jeong 한국식물병리학회 식물병연구 5 Pages
한국식물병리학회 식물병연구 2021, 27권 2호 6 79-83 (5 pages)
apple leaves. The resulting reverse transcription recombinase poly- merase amplification (RT-RPA) assay can be completed in 10 min at 42oC, is 10 times more sensitive than con- ventional reverse transcription polymerase chain reaction, and can specifically amplify ASSVd without any cross-reactivity with other common apple viruses, including apple stem grooving virus, apple stem pitting virus, and apple chlorotic leaf spot virus. The reliability of the RT-RPA assay was assessed, and the findings... -
벼 흰잎마름병의 신속하고 간편한 진단을 위한 Recombinase Polymerase Amplification 등온증폭법
김신화, 이봉춘, 김현주, 최수연, 서수좌, 김상민 한국식물병리학회 식물병연구 7 Pages
한국식물병리학회 식물병연구 2020, 26권 4호 1 195-201 (7 pages)
벼 흰잎마름병 RPA 진단법은 39oC에서 5분간만 반응하면 목표유전자의 증폭이 이루어지므로 기존 진단법보다 신속하고 간편하며 우리나라에 존재하는 K1–K3a의 4종 레이스에모두 적용가능하며, DNA 추출 없이 식물체 잎의 즙액으로 증폭반응 수행이 가능하며 기존 Taq 기반 PCR보다 약 10배 검출민감도가 높고 고가의 thermal cycler 없이도 항온수조, 발열블록 혹은 체온을 이용한 증폭반응 수행이 가능하다. 벼흰잎마름병은 벼의 농작물재해보험 대상재해 병충해 중의 하나이므로과학적 진단결과가 요구되나, 일선 농촌지도... -
감자 역병의 친환경 방제를 위한 키토산 제형의 살포
장태현, 김병섭 한국식물병리학회 식물병연구 11 Pages
한국식물병리학회 식물병연구 2012, 18권 4호 11 338-348 (11 pages)
