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The Effect of Indomethacin on the Production of Eicosanoids and Edema during Ischemia-Reperfusion Injury in Skeletal Muscle
YoonJaeChung, ByungKyuSohn, KwangSoonHyun, SangHeeYoo, HyongKyunRyu, HyungGunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.6 11 515-523 (9 pages)
During reperfusion of skeletal muscle after ischemia, lipid mediators, mainly eicosanoids, are released and may have a role in the pathogenesis of reperfusion injury. To validate the role of eicosanoids in the ischemia-reperfusion induced functional deficits in skeletal muscle, we compared muscle edema and the changes of eicosanoid concentration in the rat hind limb after ischemia-reperfusion injury by application of tourniquet. After 4 hours of ischemia, reperfusion was established for 4 hours... -
Effects of Calcium Channel Blockers on Porcine Cardiac and Coronary Arterial Function in Ischemia-Reperfusion
YungHongBaik, HyunKook, SunHeePark, SeongJooJeong, DongYoonLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.6 7 587-595 (9 pages)
This study was designed to investigate effects of calcium antagonists on endothelial and neuronal dysfunction of right coronary artery (RCA) induced by ischemia- reperfusion in anesthetized, open-chest pigs. After reperfusion, pigs were sacrificed and the RCA was rapidly dissected for in vitro experiments. Experimental groups were divided into 4 groups: control (C-RCA), ischemia-reperfusion only (I-RCA), verapamil infusion (VI-RCA) and nifedipine infusion (NI-RCA) group, respectively. The... -
Lipid Peroxidation, NF-κB Activation and Cytokine Production in Neutrophil-Stimulated Pancreatic Acinar Cells
HyeyoungKim, JeongYeonSeo, SeHaengCho, KyungHwanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.5 9 521-528 (8 pages)
Reactive oxygen species (ROS), generated by infiltrating neutrophils, are considered as an important regulator in the pathogenesis and deveolpment of pancreatitis. The present study aims to investigate whether neutrophils primed by 4β-phorbol 12β-myristate 13α-acetate (PMA) affect the productions H2O2 and lipid peroxide (LPO), NF-κB activation and cytokine production in pancreatic acinar cells, and whether these alterations were inhibited by an antioxidant, N-acetylcysteine (NAC) and... -
Angiotensin-Converting Enzyme Gene Polymorphism is not Associated with Myocardial Infarction in Koreans
SeokChaiDong-RyulSohn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.5 13 645-650 (6 pages)
To assess the relationship between angiotensin-converting enzyme (ACE) gene polymorphism and myocardial infarction in Koreans, we recruited 112 healthy, unrelated subjects (mean age 53.4 years) and 104 myocardial infarction survivors (mean age 54.2 years) of both sexes. An insertion/deletion (I/D) polymorphism of the ACE gene was typed by polymerase chain reaction. The I allelic frequency of ACE gene in Korean subjects was irrelavant to myocardial infarction (patients, 65%; control subjects... -
Effect of the Inhibition of PLA2 on Oxidative Lung Injury Induced by Interleukin-1
YoungManLee, HyunGugCho, Yoon-YubPark, Jong-KiKim, YoonJeongLee, Won-HarkPark, TeoanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.5 10 617-628 (12 pages)
In order to understand the pathogenetic mechanism of adult respiratory distress syndrome (ARDS), the role of phospholipase A2 (PLA2) in association with oxidative stress was investigated in rats. Interleukin-1 (IL-1, 50 ㄍg/rat) was used to induce acute lung injury by neutrophilic respiratory burst. Five hours after IL-1 insufflation into trachea, microvascular integrity was disrupted, and protein leakage into the alveolar lumen was followed. An infiltration of neutrophils was... -
Role of Phospholipase A2 in Oxidant-induced Alteration in Phosphate Transport in Primary Cultured Rabbit Renal Proximal Tubule Cells
KwonMooPark, SunHeeKo, JaeSukWoo, JinSupJung, SangHoLee, YongKeunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.5 8 601-609 (9 pages)
The present study was undertaken to examine the role of phospholipase A2 (PLA2) in oxidant-induced inhibition of phosphate transport in primary cultured rabbit renal proximal tubule cells. Uptakes of phosphate and glucose were dose-dependently inhibited by an oxidant t-butylhydroperoxide (tBHP), and the significant inhibition appeared at 0.025 mM of tBHP, whereas tBHP-induced alterations in lipid peroxidation and cell viability were seen at 0.5 mM. tBHP stimulated arachidonic acid (AA) release... -
Effect of the Inhibition of Platelet Activating Factor on Oxidative Lung Injury Induced by Interleukin-1α
YoungManLeeYoon-YubPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 13 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.4 10 479-491 (13 pages)
In order to know the pathogenesis of adult respiratory distress syndrome (ARDS) in association with the oxidative stress by neutrophils, the role of platelet activating factor (1-0-alkyl-2-acetyl-sn- glycero-3-phosphocholine, PAF) was investigated during acute lung injury induced by interleukin- 1α (IL-1) in rats. An insufflation of IL-1 into the rat's trachea increased the acetyltransferase activity in the lung and the increase of PAF content was followed. As evidences of acute lung injury by... -
The Inhibitory Mechanism of Aloe Glycoprotein (NY945) on the Mediator Release in the Guinea Pig Lung Mast Cell Activated with Antigen-Antibody Complexes
JaiYoulRo, ByungChulLee, MyungHeeChung, SeungKiLee, ChungKiSung, KyungHwanKim, YoungInPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 13 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.1 15 119-131 (13 pages)
It has been reported that the g1ycoprotein extracted from A10e has strong anti-inflammatory response， However， there has been no research report yet about the effect of A10e on allergic hypersensitivity reactivity， By using guinea pig 1ung mast cells， this study aimed to examine the effects of A10e g1ycoprotein (NY945) on the mediator re1eases caused by mast cell activation， and a1so aimed to assess the effects of NY945 on the mechanism of mediator re1eases in the mast cell activation. We... -
Platelet-Activating Factor Enhances Interleukin-1 Activity by Alveolar Macrophages Inhibition by PAF Specific Receptor Antagonists
JiheeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.2 10 201-208 (8 pages)
It is becoming increasingly clear that the inflammatory reaction can be ascribed to a complex array of mediators generated and released from activated phagocytes. In this study, the effect of PAF on interleukin-1(IL-1) activity by rat alveolar macrophages(AM) was examined using thymocyte proliferation assay in the supernate of sample obtained after 24 hr culture. When AM were cultured with PAF alone, no change in IL-1 activity was observed. However, the combined addition of PAF and muramyl... -
Inhibition of Phospholipase A2 Diminishes the Acute Alveolar Injury Induced by Interleukin-1
YoungManLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.1 9 71-78 (8 pages)
In an attempt to investigate the role of phospholipase A2(PLA2) in interleukin-1 (IL-1) induced acute lung injury, mepacrine was tried to inhibit PLA2 in IL-1 induced ARDS rats. For confirmation of acute lung injury by IL-1, and to know the role of neutrophils in this injury, lung leak index, lung myeloperoxidase(MPO), number of neutrophils and protein content in the bronchoalveolar lavage (BAL) and wet lung weight were measured. At the same time lung PLA2 was measured to know the effect of...
