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Development of immunoassays for the detection of kanamycin in veterinary fields
Yong Jin, Jin-Wook Jang, Chang-Hoon Han, Mun-Han Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 3 111-117 (7 pages)
Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides, indicating that the monoclonal antibody was highly specific for kanamycin. Based on competitive direct ELISA, the detection limits of... -
Influence of ascorbic acid on BUN, creatinine, resistive index in canine renal ischemia-reperfusion injury
Jae-il Lee, Myung-jin Kim, Chang-sik Park, Myung-cheol Kim 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 14 79-81 (3 pages)
Renal ischemia as a course of renal transplantation is a common cause of renal dysfunction as renal failure.The purpose of this study was to investigate the influence of ascorbic acid on blood urea nitrogen (BUN), creatinine(Cr)and resistive index (RI)for dog models with renal ischemia-reperfusion (I/R) injury.Renal ischemia was induced on 6 Beagle dogs.The left kidney was exposed to normothermic ischemia for a short period at 30 min followed by reperfusion.On the blood Cr level and RI, there... -
Characterization of Brachyspira hyodysenteriae isolates from Korea
Tae Jung Kim, Suk Chan Jung, Jae Il Lee 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 12 335-339 (5 pages)
This study was done to characterize diversity in 10 Brachyspira hyodysenteriae isolates in Korea. The isolates were compared with 14 well-characterized non-Korean strains of various Brachyspira species. All Korean isolates showed strong beta haemolysis and had blunt cell ends with 7~14 periplasmic flagella. They produced indole, and did not ferment fructose. They were alpha-glucosidase positive and alpha-galatosidase negative using the APIZYM kit. Using polyclonal antisera raised in rabbits... -
A predictive model for the level of sIgA based on IgG levels following the oral administration of antigens expressed in Sacchromyces cerevisiae
Sung Jae Shin, Seung Won Shin, Eun Jin Choi, Deog Yong Lee, Jeong Min Ahn, Moon Sik Yang, Yong Suk Jang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 8 305-309 (5 pages)
Oral vaccination may be the most efficient way of inducing an immune response at the remote mucosal site through the common mucosal immune network. Antigenspecific secretory IgA (sIgA) is the major immunoglobulin type generally detected in the secretions of experimental animals following an effective oral immunization. Actinobacillus pleuropneumoniae causing disease in the lung of pig initially interacts, colonizes, and infects the host tissues at the mucosal surface of the respiratory tract.... -
Expression of tyrosine kinase A in the cerebral cortex of postnatal developing rat
Hyo Jung Kwon, Kyoung Youl Lee, Il Kwon Park, Mi Sun Park, Mi Young Lee, Moo Kang Kim 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 2 185-189 (5 pages)
Tyrosine kinase A (TrkA)is an essential component of the high affinity nerve growth factor (NGF)receptor necessary to the mediate the biological effects of the neurotrophins, NGF. This study examined the distribution of TrkA-immunoreactivity (IR)cells in the postnatal rat cerebral cortex and the changes that occur in postnatal development as a result of the expression of this protein. TrkA-IR was detected at postnatal day (PD)3, PD6, PD9 and PD15. Base upon their somatodendritic morphology, the... -
Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice
Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Seong-Hee Kim, Jae-Young Song, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 6 125-133 (9 pages)
Genes encoding for the premembrane and envelope (prME), envelope (E) and nonstructural protein (NS1) of Japanese encephalitis virus (JEV) were cloned. Each protein was expressed in baculovirus expression system. Of the three proteins expressed in baculovirus system, only prME had hemagglutination activity. The prME (72 and 54 kDa), E (54 kDa) and NS1 (46 kDa) proteins could be detected by Western blotting in the recombinant virus infected cells. Immunogenicity of the recombinant proteins... -
Changes in orexin-A and neuropeptide Y expression in the hypothalamus of the fasted and high-fat diet fed rats
Eun Sung Park, Seong Joon Yi, Jin Sang Kim, Heungshik S. Lee, In Se Lee, Je Kyung Seong, Hee Kyung Jin, Yeo Sung Yoon 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 3 295-302 (8 pages)
This study was aimed to investigate the changes of orexin-A (OXA) and neuropeptide Y (NPY) expression in the hypothalamus of the fasted and high-fat diet fed rats. For the experiments, the male Sprague-Dawley (SD) rats were used as the model of high-fat diet-induced obesity. The mean loss of body weight (MLBW) did not show the linear pattern during the fasting; from 24 h to 84 h of fastings, the MLBW was not significantly changed. The numbers of OXA-immunoreactive (IR) neurons were decreased at... -
Application of biotechnological tools for coccidia vaccine development
Wongi Min, Rami A. Dalloul, Hyun S. Lillehoj 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 1 279-288 (10 pages)
Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of host-parasite interactions and... -
Glucocorticoid-induced laminitis with hepatopathy in a Thoroughbred filly
Seung-ho Ryu, Byung-sun Kim, Chang-woo Lee, Junghee Yoon, Yonghoon Lyon Lee 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 15 271-274 (4 pages)
A 3-year-old Thoroughbred filly was referred to the Equine Hospital, Korea Racing Association for evaluation of hematuria, inappetite, weight loss and depression. From 25 days prior to admission, the horse was treated for right carpal lameness with 20 mg intramuscular administration of triamcinolone acetonide per day for consecutive 10 days by a local veterinarian. Clinical and laboratory findings included vaginal hyperemia, flare in bladder wall, neutrophilia, lymphopenia, polyuria, polydipsia... -
Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea
Dong Kun Yang, Byoung Han Kim, Chang Hee Kweon, Jun Hun Kwon, Seong In Lim, Hong Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 3 197-205 (9 pages)
strain and the highest amino acid homology with K94P05. We performed an extensive E gene based phylogenetic analysis on a selection of 41 JEV isolates available from the GenBank. Compared with Anyang strain, isolated from a pig in 1969, that is current live vaccine strain for swine in Korea, the homology of nucleotide sequence in envelope gene was only 87.1%. The prM gene of the isolate was closely related with those of Ishikawa and K94P05 strains, which were grouped into genotype I of JEV.


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