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In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes
Momena Khatun, Mohammad Musharraf Uddin Bhuiyan, Jalal Uddin Ahmed, Aminul Haque, Mohammad Bozlur Rahman*, Mohammed Shamsuddin 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 11 75-82 (8 pages)
Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle's salt with L-glutamine and sodium bicarbonate) for 27 h at 39C under 5% CO2 in humidified air. The... -
In vitro toxicity of melamine against Tetrahymena pyriformis cells
Zhengfang Wang, Liben Chen, Rashad Al-Kasir, Bo Han* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 5 27-34 (8 pages)
This study assessed the toxicity of melamine against the unicellular eukaryotic system of Tetrahymena (T.) pyriformis exposed to 0, 0.05, 0.25, 0.5, 2.5, and 5 mg/mL of melamine. Cell growth curves of different cultures, the half maximum inhibition concentration (IC50) value of melamine, and morphological changes in cells were obtained via optical and transmission electron microscopic observation. The effects of eleven melamine concentrations, 0.25, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5... -
Determination of angiotensin I-converting enzyme activity in equine blood: lack of agreement between methods of analysis
Maria Fernanda de M. Costa*, Adriana K. Carmona, Marcio F. M. Alves, Timothy M. Ryan, Helen M. Davies, Garry A. Anderson, Ron F. 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 4 21-25 (5 pages)
Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard"... -
Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties
H. ?lbegi-Mohyla, M. Hijazin, J. Alber, C. L?mmler, A. A. Hassan, A. Abdulmawjood, E. Prenger-Berninghoff, R. Weiß, M. Zsch?ck 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 13 265-267 (3 pages)
species identity of the two A. pyogenes strains could additionally be confirmed by PCR mediated amplification of species specific parts of the 16S-23S rDNA intergenic spacer region, the pyolysin encoding gene plo and by amplification of the collagen-binding protein encoding gene cbpA. All these molecular targets might help to improve the future identification and further characterization of A. pyogenes which, as demonstrated in the present study, could also be isolated from reptile specimens. -
Autoantibodies against thyroid hormones and their influence on thyroxine determination with chemiluminescence immunoassay in dogs
Marion Piechotta*, Michael Arndt, Hans-Otto Hoppen 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 3 191-196 (6 pages)
(n = 691); Group 4: biochemical euthyroid (n = 138); Group 5: hypothyroid dogs under substitution therapy (n = 141); Group 6: healthy dogs (n = 110). The incidence of T4AA and T3AA, determined using radiometric assay, was low (0.5% and 3.8%) and higher in hypothyroid dogs compared to dogs suspicious for hypothyroidism (Group 2-4) (p＜0.05). T4AA was not detected in dogs with normal T4 and elevated TSH. T4 concentrations of T4AA positive samples determined using HPLC were comparable to results... -
Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR
Silvia Preziuso, *, Fulvio Laus, Aurora Romero Tejeda, Carlo Valente, Vincenzo Cuteri2 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 10 67-72 (6 pages)
Streptococcus (S.) dysgalactiae subsp. equisimilis is responsible for severe diseases in humans, including primary bacteraemia, pneumonia, endocarditis, and toxic shock syndrome. Infection in some animal species can also occur, although a few studies have looked into cross-species infectivity. In horses, S. equisimilis is generally considered infrequent or opportunistic, but has recently been isolated from cases of strangles-like disease. Rapid and sensitive diagnostic techniques could enable... -
Induction of oxidative stress and lipid peroxidation in rats chronically exposed to cypermethrin through dermal application
Rajinder Raina, Pawan K. Verma, N. K. Pankaj, Shahid Prawez* 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 12 257-259 (3 pages)
Present study was undertaken to study the effect of cypermethrin on oxidative stress after chronic dermal application. The insecticide was applied dermally at 50 mg/kg body weight in different groups of Wistar rats of either sex weighing 150∼200 g. Significant (p ＜ 0.05) increase in catalase activity was observed after 30 days of exposure. However, the superoxide dismutase activity declined significantly after 60 days of exposure. The activity of glutathione peroxidase and blood... -
Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl3-induced toxicity in the rat forebrain cortex
Ivana D. Stevanović*, Marina D. Jovanović, Ankica Jelenković, Miodrag Čolić, Ivana Stojanović, Mil 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 3 15-22 (8 pages)
forebrain cortices were used for the biochemical analyses: nitrite levels, superoxide production, malondialdehyde concentrations, superoxide dismutase (SOD) activities and reduced glutathione contents. AlCl3 injection resulted in increased nitrite concentrations, superoxide anion production, malondialdehyde concentrations and reduced glutathione contents in the forebrain cortex, suggesting that AlCl3 exposure promoted oxidative stress in this brain structure. The biochemical changes observed in... -
Immunohistochemical study of galectin-3 in mature and immature bull testis and epididymis
Hwanglyong Kim, Meejung Ahn, Changjong Moon, Seungjoon Kim, Youngheun Jee, Hong-Gu Joo, Taekyun Shin* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 1 339-344 (6 pages)
Galectin-3, a member of the b-galactoside-binding protein family, has been implicated in mammalian sperm maturation. We examined galectin-3 expression in the testis and epididymis of sexually mature and immature bulls. Western blot analysis showed varying levels of galectin-3 in the bull testis and epididymis, and galectin-3 immunoreactivity was higher in the mature testis and epididymis than in immature organs. Galectin-3 was primarily localized in interstitial cells of the immature bull testis... -
All blood, No stool: enterohemorrhagic Escherichia coli O157:H7 infection
Jang W. Yoon, Carolyn J. Hovde 대한수의학회 Journal of Veterinary Science 14 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 1 219-232 (14 pages)
Enterohemorrhagic Escherichia coli serotype O157:H7 is a pathotype of diarrheagenic E. coli that produces one or more Shiga toxins, forms a characteristic histopathology described as attaching and effacing lesions, and possesses the large virulence plasmid pO157. The bacterium is recognized worldwide, especially in developed countries, as an emerging food-borne bacterial pathogen, which causes disease in humans and in some animals. Healthy cattle are the principal and natural reservoir of E....
