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231.

Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

Seong In Lim, Wooseog Jeong, Dong Seob Tark, Dong Kun Yang, Chang Hee Kweon* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 9 331-336 (6 pages)

Seong In Lim, Wooseog Jeong, Dong Seob Tark, Dong Kun Yang, Chang Hee Kweon* 저
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- 3,000원
Bovine leukemia virus (BLV) envelope glycoprotein (gp51/ gp30T-), consisting of BLV gp51 and BLV gp30 that lacked its C-terminal transmembrane domain, was expressed in insect cells under the control of the baculovirus polyhedron promoter. Recombinant BLV gp51/gp30T- secreted from insect cells was determined by immunofluorescence, enzyme- linked immunosorbent and western blot assays using a BLV-specific monoclonal antibody and BLV-positive bovine antibodies. An agar gel immunodiffusion (AGID)...
232.

Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand

Tawatchai Pohuang, Niwat Chansiripornchai*, Achara Tawatsin, Jiroj Sasipreeyajan 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 7 219-223 (5 pages)

Tawatchai Pohuang, Niwat Chansiripornchai*, Achara Tawatsin, Jiroj Sasipreeyajan 저
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- 1,500원
Thirteen field isolates of infectious bronchitis virus (IBV) were isolated from broiler flocks in Thailand between January and June 2008. The 878-bp of the S1 gene covering a hypervariable region was amplified and sequenced. Phylogenetic analysis based on that region revealed that these viruses were separated into two groups (I and II). IBV isolates in group I were not related to other IBV strains published in the GenBank database. Group 1 nucleotide sequence identities were less than 85% and...
233.

Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms

Hye Kwon Kim, Jeong Sun Yang, Hyoung Joon Moon, Seong Jun Park, Yuzi Luo, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Soo Kyung A 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 5 121-130 (10 pages)

Hye Kwon Kim, Jeong Sun Yang, Hyoung Joon Moon, Seong Jun Park, Yuzi Luo, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Soo Kyung A 저
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- 3,000원
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic...
234.

Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis

Jung-Min Yon, In-Jeoung Baek, Se-Ra Lee, Mi-Ra Kim, Beom Jun Lee, Young Won Yun, Sang-Yoon Nam 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 2 233-240 (8 pages)

Jung-Min Yon, In-Jeoung Baek, Se-Ra Lee, Mi-Ra Kim, Beom Jun Lee, Young Won Yun, Sang-Yoon Nam 저
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- 3,000원
The signal in embryos was observed at the lowest level on ED 9.5-11.5, and the highest level on ED 17.5-18.5, while levels remained constant in the surrounding extraembryonic tissues during all developmental stages examined. Immunohistochemical analysis of SOD1 expression on ED 13.5-18.5 revealed its ubiquitous distribution throughout developing organs. In particular, high levels of SOD1 expression were observed in the ependymal epithelium of the choroid plexus, ganglia, sensory cells of the...
235.

Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do, Korea

Bo-Young Jeon, Sungmo Je, Jinhee Park, Yeun Kim, Eun-Gae Lee, Hyeyoung Lee, Sangkyo Seo, Sang-Nae Cho* 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 4 145-154 (10 pages)

Bo-Young Jeon, Sungmo Je, Jinhee Park, Yeun Kim, Eun-Gae Lee, Hyeyoung Lee, Sangkyo Seo, Sang-Nae Cho* 저
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- 3,000원
a molecular technique, the variable number tandem repeat (VNTR) typing method, to identify and distinguish the M. bovis isolates from Gyeonggi-do, Korea. From 2003 to 2004, 59 M. bovis clinical strains were isolated from dairy cattle in Gyeonggi-do, Korea, and these cattle had tuberculosis-like lesions. Twenty-four published MIRU- VNTR markers were applied to the M. bovis isolates and ten of them showed allelic diversity. The most discriminatory locus for the M. bovis isolates in Korea was QUB...
236.

Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea

Seong Joon Joh, *, Chae Ik Shon, Sung Won Kang, Byoung Han Kim, Byung Yul Jeong, Kyung Gi Lee, Jun Hun Kwon, Gang Jun Heo2 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 11 85-90 (6 pages)

Seong Joon Joh, *, Chae Ik Shon, Sung Won Kang, Byoung Han Kim, Byung Yul Jeong, Kyung Gi Lee, Jun Hun Kwon, Gang Jun Heo2 저
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- 3,000원
The cDNA nucleotide sequence of genome segment B encoding the VP1 protein was determined for the aquatic birnavirus GC1 isolated from the rockfish Sebastes schlegeli in Korea. The VP1 protein of GC1 contains a 2,538 bp open reading frame, which encodes a protein comprising 846 amino acid residues that has a predicted MW of 94 kDa. The sequence contains 6 potential Asn-X-Ser/Thr motifs. Eight potential Ser phosphorylation sites and 1 potential Tyr phophorylation site were also identified. GC1...
237.

Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea

Ji-Hyun Jang, Haan-Woo Sung, Chang-Seon Song, Hyuk-Moo Kwon* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 13 401-408 (8 pages)

Ji-Hyun Jang, Haan-Woo Sung, Chang-Seon Song, Hyuk-Moo Kwon* 저
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- 3,000원
carriers of infectious bronchitis between 2001 and 2003. The S1 glycoprotein genes of these IBV isolates were amplified by reverse transcriptase-polymerase chain reaction (RT- PCR) and analyzed by restriction fragment length polymorphism (RFLP) analysis. These Korean IBV isolates were classified into three groups according to their RFLP patterns obtained using the restriction enzyme HaeIII. Half of the twelve isolates were similar to the KM91 RFLP pattern, which is a common pattern in Korea....
238.

Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro

Tae S. Jung, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 9 255-261 (7 pages)

Tae S. Jung, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 저
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- 3,000원
bacteria cultured in TSB or GRM. When bacteria were passaged several times on tryptic soya agar prior to culturing in the four different media, only bands at 24 and 47 kDa were recognized, regardless of the medium used to culture the bacteria. It would appear that the molecular weight of Ph. d. subsp. piscicida antigens change in the presence of iron restriction, and sera from sea bass infected with live bacteria are able to detect epitopes on the antigens after this shift in molecular weight.
239.

Antimicrobial susceptibility and molecular detection of chloramphenicol and florfenicol resistance among Escherichia coli isolates from diseased chickens

Xin-Sheng Li, Gui-Qin Wang, Xiang-Dang Du, Bao-An Cui, Su-Mei Zhang, Jian-Zhong Shen 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 7 243-247 (5 pages)

Xin-Sheng Li, Gui-Qin Wang, Xiang-Dang Du, Bao-An Cui, Su-Mei Zhang, Jian-Zhong Shen 저
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- 1,500원
(100%), oxytetracycline (100%), ampicillin (83%), enrofloxacin (83%), and ciprofloxacin (81%), respectively. Among the phenicols, resistance was approximately 79% and 29% for chloramphenicol and florfenicol, respectively. Molecular detection revealed that the incidence rates of the floR, cmlA, cat1, cat2 and cat3 were 29, 31, 16, 13, and 0%, respectively. Additionally, 10% of the isolates were positive for both floR and cmlA. As these antimicrobial agents may potentially induce cross-resistance...
240.

Diversity of swine Bordetella bronchiseptica isolates evaluated by RAPD analysis and PFGE

Eun-Kyung Shin, Yeon-Soo Seo, Jeong Hee Han, Tae-Wook Hahn 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 10 65-73 (9 pages)

Eun-Kyung Shin, Yeon-Soo Seo, Jeong Hee Han, Tae-Wook Hahn 저
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- 3,000원
41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. The OPA-07, OPA-08 and OPA-18 primers yielded 10, 10, and 6 distinct fingerprint patterns, respectively. The most common identical RAPD pattern was produced by OPA- 07 which was shared by 32 isolates (71.1%), the pattern produced by...

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