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251.

Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 14 315-322 (8 pages)

Ann-Kathrin Diercks, Heinrich F. B?rgers, Anna Schwab, Johannes Schenkel* 저
...
- 3,000원
Genetically modified (GM) animals are unique mutants with an enormous scientific potential. Cryopreservation of pre-implantation embryos or spermatozoa is a common approach for protecting these lines from being lost or to store them in a repository. A mutant line can be taken out of a breeding nucleus only if sufficient numbers of samples with an appropriate level of quality are cryopreserved. The quality of different donors within the same mouse line might be heterogeneous and the...
252.

Frequency of group A rotavirus with mixed G and P genotypes in bovines: predominance of G3 genotype and its emergence in combination with G8/G10 types

Yashpal S. Malik*, Kuldeep Sharma, Nirupama Vaid, Somendu Chakravarti, K. M. Chandrashekar, Sanjay S. Basera, Rashmi Singh, Mina 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 8 271-278 (8 pages)

Yashpal S. Malik*, Kuldeep Sharma, Nirupama Vaid, Somendu Chakravarti, K. M. Chandrashekar, Sanjay S. Basera, Rashmi Singh, Mina 저
...
- 3,000원
to 100% with other bovine G3 strains. The highest identity (98.9% to 100%) was observed with RUBV3 bovine G3 strains from eastern India. The G3 strains (B-46, 0970, and BR-133) showed 97.5% to 98.8% sequence homologies with the Indian equine RV strain Erv-80. Phylogenetic analysis demonstrated that G3 strains clustered with bovine RUBV3 and J-63, and equine Erv-80 G3. Overall, these results confirmed that the incidence of infection by RVs with the G3 genotype and mixed genotypes in the bovine...
253.

Pathogen translocation and histopathological lesions in an experimental model of Salmonella Dublin infection in calves receiving lactic acid bacteria and lactose supplements

Laureano S. Frizzo*, Maria V. Zbrun, Lorena P. Soto, Ezequiel Bertozzi, Gabriel J. Sequeira, Luis E. Marti, Marcelo L. Signorini 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 7 261-270 (10 pages)

Laureano S. Frizzo*, Maria V. Zbrun, Lorena P. Soto, Ezequiel Bertozzi, Gabriel J. Sequeira, Luis E. Marti, Marcelo L. Signorini 저
...
- 3,000원
each strain throughout the experiment. Lactose was provided to the L-LAB-G in doses of 100 g/day. Salmonella Dublin (2 × 10 CFU) was orally administered to all animals on day 11 of the experiment. The microscopic lesion index values in target organs were 83%, 70%, and 64.3% (p ＜ 0.05) for the C-G, LAB-G, and L-LAB-G, respectively. Administration of the probiotic inoculum was not fully effective against infection caused by Salmonella. Although probiotic treatment was unable to delay the arrival...
254.

Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times

Miao Zhang, Zhenhua Yue, Zhijun Liu, Ali Islam, Buriro Rehana, Shu Tang, Endong Bao*, Jorg Hartung 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 6 253-259 (7 pages)

Miao Zhang, Zhenhua Yue, Zhijun Liu, Ali Islam, Buriro Rehana, Shu Tang, Endong Bao*, Jorg Hartung 저
...
- 3,000원
The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70...
255.

Evaluation of modified vaccinia virus Ankara expressing VP2 protein of infectious bursal disease virus as an immunogen in chickens

Flavia Adriana Zanetti*, Mar?a Paula Del M?dico Zajac, Oscar Alberto Taboga, Gabriela Calamante 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 12 199-201 (3 pages)

Flavia Adriana Zanetti*, Mar?a Paula Del M?dico Zajac, Oscar Alberto Taboga, Gabriela Calamante 저
...
- 1,500원
A recombinant modified vaccinia Ankara (MVA) virus expressing mature viral protein 2 (VP2) of the infectious bursal disease virus (IBDV) was constructed to develop MVA-based vaccines for poultry. We demonstrated that this recombinant virus was able to induce a specific immune response by observing the production of anti-IBDV-seroneutralizing antibodies in specific pathogen-free chickens. Besides, as the epitopes of VP2 responsible to induce IBDV-neutralizing antibodies are discontinuous, our...
256.

In vitro susceptibility of Staphylococcus aureus strains isolated from cows with subclinical mastitis to different antimicrobial agents

Ayman El Behiry*, Gerd Schlenker, Istvan Szabo, Uwe Roesler 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 6 153-161 (9 pages)

Ayman El Behiry*, Gerd Schlenker, Istvan Szabo, Uwe Roesler 저
...
- 3,000원
a significant reduction in susceptibility to the nonoxinol-9 iodine complex but only one strain developed resistance to chlorhexidine digluconate. Stability of the acquired resistance was observed in all S. aureus strains adapted to the nonoxinol-9 iodine complex and chlorhexidine digluconate. In contrast, simultaneous resistance to different antibiotics was not observed in any of the ten investigated S. aureus strains. However, the isolates exhibited a high degree of resistance to penicillin G....
257.

Pathotyping avian pathogenic Escherichia coli strains in Korea

Yong-Wun Jeong, Tae-Eun Kim, Jae-Hong Kim*, Hyuk-Joon Kwon* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 5 145-152 (8 pages)

Yong-Wun Jeong, Tae-Eun Kim, Jae-Hong Kim*, Hyuk-Joon Kwon* 저
...
- 3,000원
To examine the genetic background of avian pathogenic Escherichia coli (APEC) that affects virulence of this microorganism, we characterized the virulence genes of 101 APEC strains isolated from infected chickens between 1985∼2005. Serotypes were determined with available anti-sera and median lethal doses were determined in subcutaneously inoculated chicks. The virulence genes we tested included ones encoding type 1 fimbriae (fimC), iron uptake-related (iroN, irp2, iucD, and fyuA), toxins (lt,...
258.

Molecular characterization of Escherichia coli O157:H7 strains isolated from different sources and geographic regions

Adriana Hamond Regua-Mangia*, Alice Gon?alves M. Gonzalez, Aloysio M. F. Cerqueira, Joao Ramos C. Andrade 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 4 139-144 (6 pages)

Adriana Hamond Regua-Mangia*, Alice Gon?alves M. Gonzalez, Aloysio M. F. Cerqueira, Joao Ramos C. Andrade 저
...
- 3,000원
Escherichia (E.) coli serotype O157:H7 is a globally distributed human enteropathogen and is comprised of microorganisms with closely related genotypes. The main reservoir for this group is bovine bowels, and infection mainly occurs after ingestion of contaminated water and food. Virulence genetic markers of 28 O157:H7 strains were investigated and multilocus enzyme electrophoresis (MLEE) was used to evaluate the clonal structure. O157:H7 strains from several countries were isolated from food,...
259.

Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle

Sangho Choi, Hee-Jong Woo*, Jeongmin Lee* 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 3 127-137 (11 pages)

Sangho Choi, Hee-Jong Woo*, Jeongmin Lee* 저
...
- 4,000원
Bovine spongiform encephalopathy (BSE) is one of the fatal neurodegenerative diseases known as transmissible spongiform encephalopathies (TSEs) caused by infectious prion proteins. Genetic variations correlated with susceptibility or resistance to TSE in humans and sheep have not been reported for bovine strains including those from Holstein, Jersey, and Japanese Black cattle. Here, we investigated bovine prion protein gene (PRNP) variations in Hanwoo cattle [Bos (B.) taurus coreanae], a native...
260.

Isolation and genetic characterization of Japanese encephalitis virus from equines in India

Baldev R. Gulati*, Harisankar Singha, Birendra K. Singh, Nitin Virmani, Sanjay Kumar, Raj K. Singh 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 1 111-118 (8 pages)

Baldev R. Gulati*, Harisankar Singha, Birendra K. Singh, Nitin Virmani, Sanjay Kumar, Raj K. Singh 저
...
- 3,000원
Japanese encephalitis (JE) is an important vector-borne viral disease of humans and horses in Asia. JE outbreaks occur regularly amongst humans in certain parts of India and sporadic cases occur among horses. In this study, JE seroprevalence and evidence of JE virus (JEV) infection among horses in Haryana (India) is described. Antibodies against JEV were detected in 67 out of 637 (10.5%) horses screened between 2006 and 2010. Two foals exhibiting neurological signs were positive for JEV RNA by...

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