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A new recombined porcine reproductive and respiratory syndrome virus virulent strain in China
Jian-guo Dong, Lin-yang Yu, Pei-pei Wang, Le-yi Zhang, Yan-ling Liu, Peng-shuai Liang, Chang-xu Song 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 12 89-98 (10 pages)
Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases worldwide. In the present study, a new virulent strain of PRRS virus (PRRSV), GDsg, was isolated in Guangdong province, China, and caused high fever, high morbidity, and high mortality in sows and piglets. The genome of this new strain was 15,413 nucleotides (nt) long, and comparative analysis revealed that GDsg shared 82.4% to 94% identity with type 2 PRRSV strains, but only 61.5% identity with type... -
Isolation and characterization of a new porcine epidemic diarrhea virus variant that occurred in Korea in 2014
Dong-Kun Yang, Ha-Hyun Kim, Seung-Heon Lee, Soon-Seek Yoon, Jung-Won Park, In-Soo Cho 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 10 71-78 (8 pages)
Outbreaks of porcine epidemic diarrhea (PED) have resulted in significant economic losses in the swine industry, and another PED outbreak occurred in 2014 in Korea. Isolating and culturing PED virus (PEDV) allow investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated two PEDV isolates (QIAP1401 and QIAP1402) from naturally infected piglets at Jeju-do, Korea. Viral propagation was confirmed in Vero cells based on... -
Comparative proteomic analysis of outer membrane protein 43 (omp43)-deficient Bartonella henselae
Jun-Gu Kang, Hee-Woo Lee, Sungjin Ko, Joon-Seok Chae 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 9 59-70 (12 pages)
Outer membrane proteins (OMPs) of Gram-negative bacteria constitute the first line of defense protecting cells against environmental stresses including chemical, biophysical, and biological attacks. Although the 43-kDa OMP (OMP43) is major porin protein among Bartonella henselae-derived OMPs, its function remains unreported. In this study, OMP43-deficient mutant B. henselae (Δomp43) was generated to investigate OMP43 function. Interestingly, Δomp43 exhibited weaker proliferative ability than... -
Analysis of structure-function relationship in porcine rotavirus A enterotoxin gene
Sharad Saurabh, Shubhankar Sircar, Jobin J. Kattoor, Souvik Ghosh, Nobumichi Kobayashi, Krisztian Banyai, Obli R. VinodhKumar, U 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 6 35-43 (9 pages)
Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the nsp4 gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of nsp4 in the porcine population. Full-length nsp4 genes... -
Piglet colibacillosis diagnosis based on multiplex polymerase chain reaction and immunohistochemistry of paraffin-embedded tissues
C?ntia De Lorenzo, Caroline P. de Andrade, Ver?nica S. L. Machado, Matheus V. Bianchi, Veronica M. Rolim, Raquel A. S. Cruz, Dav 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 5 27-33 (7 pages)
Enterotoxigenic Escherichia coli (ETEC) causes diarrhea in pigs, referred to as colibacillosis. The aim of this study was to optimize multiplex polymerase chain reaction (PCR) and immunohistochemistry (IHC) analyses of paraffin-embedded material to detect pathogenic E. coli strains causing colibacillosis in pigs. Multiplex PCR was optimized for fimbriae (F18, F4, F6, F5, and F41) and toxins (types A and B heat-stable toxins [STaP and STb], heat-labile toxin [LT], and type 2 Shiga toxin [STx2e]),... -
Coactosin-like protein 1 inhibits neuronal migration during mouse corticogenesis
Guohong Li, Yupeng Yin, Jiong Chen, Yanle Fan, Juhong Ma, Yingxue Huang, Chen Chen, Pengxiu Dai, Shulin Chen, Shanting Zhao 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 4 21-26 (6 pages)
Coactosin-like protein 1 (Cotl1), a member of the actin-depolymerizing factor (ADF)/cofilin family, was first purified from a soluble fraction of Dictyostelium discoideum cells. Neuronal migration requires cytoskeletal remodeling and actin regulation. Although Cotl1 strongly binds to F-actin, the role of Cotl1 in neuronal migration remains undescribed. In this study, we revealed that Cotl1 overexpression impaired migrationof both early- and late-born neurons during mouse corticogenesis.... -
Infections by pathogens with different transmission modes in feral cats from urban and rural areas of Korea
Jusun Hwang, Nicole Gottdenker, Dae-Hyun Oh, Hang Lee, Myung-Sun Chun 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 14 541-545 (5 pages)
In this study, we examine prevalences of three infectious pathogens with different transmission modes (Bartonella henselae, hemoplasma, and Toxoplasma gondii) in feral cats from urban and rural habitats. Infection status of the three pathogens in blood samples (n = 117) was determined through molecular or serological diagnostic methods. Overall prevalence of hemoplasma, Toxoplasma gondii, and Bartonella henselae was 47.9%, 50%, and 35.7%, respectively. Comparing the two habitats, only... -
Evaluation of expression of the Wnt signaling components in canine mammary tumors via RT Profiler PCR Array and immunochemistry assays
Fang Yu, Roberta Rasotto, Hong Zhang, Shimin Pei, Bin Zhou, Xu Yang, Yipeng Jin, Di Zhang, Degui Lin 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 13 359-367 (9 pages)
The Wnt signaling pathway and its key component β-catenin have critical roles in the development of diseases such as tumors in mammals. However, little has been reported about involvement of the Wnt/β-catenin signaling pathway in canine mammary tumors (CMTs). The present study detected expression of 30 Wnt signaling pathway-related genes in CMTs; the results are potentially useful for molecular-based diagnosis of CMTs and the development of new targeted therapies. Significant upregulations of... -
Effect of phosphorus deficiency on erythrocytic morphology and function in cows
Ziwei Zhang, Mingyu Bi, Jie Yang, Haidong Yao, Zhonghua Liu, Shiwen Xu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 10 333-340 (8 pages)
The aim of this study was to evaluate the influence of phosphorus (P) deficiency on the morphological and functional characteristics of erythrocytes in cows. Forty Holstein-Friesian dairy cows in mid-lactation were randomly divided into two groups of 20 each and were fed either a low-P diet (0.03% P/kg dry matter [DM]) or a control diet (0.36% P/kg DM). Red blood cell (RBC) indices results showed RBC and mean corpuscular hemoglobin decreased while mean corpuscular volume increased significantly... -
Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014
Qiwen Wu, Zhili Li, Guanqun Zhang, Jianqiang Niu, Xiduo Zeng, Baoli Sun, Jingyun Ma 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 8 317-326 (10 pages)
Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. In this study, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in southern China. We analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in southern China during 2007?2014. The alignment results showed that the nucleotide identity similarities of the two genes among these strains were 80.5%?99.7% and 80.9%?100%,...


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