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본문검색 결과만 보기
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Rapid Detection of Lily mottle virus and Arabis mosaic virus Infecting Lily (Lilium spp.) Using Reverse Transcription Loop-Mediated Isothermal Amplification
Yubao Zhang, Yajun Wang, Zhongkui Xie, Ruoyu Wang, Zhihong Guo, Yuhui He 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 2호 6 170-178 (9 pages)
to develop simple, sensitive, and reliable detection methods for these two viruses to prevent them from spreading. Reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assays have been de- veloped to detect LMoV and ArMV using two primer pairs that match six conserved sequences of LMoV and ArMV coat proteins, respectively. RT-LAMP assay results were visually assessed in reaction tubes using green fluorescence and gel electrophoresis. Our assays successfully detected both LMoV... -
Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification
Yubao Zhang, Zhongkui Xie, John D Fletcher, Yajun Wang, Ruoyu Wang, Zhihong Guo, Yuhui He 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 7 76-86 (11 pages)
plants without cross reactivity recorded from either CMV or LNYV or four other related plant viruses. Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg2+ at 65°C for LNYV and 60°C for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction... -
Race- and Isolate-specific Molecular Marker Development through Genome- Realignment Enables Detection of Korean Plasmodiophora brassicae Isolates, Causal agents of Clubroot Disease
Ji-Yun Jeong, Arif Hasan Khan Robin, Sathishkumar Natarajan, Rawnak Laila, Hoy-Taek Kim, Jong-In Park, Ill-Sup Nou 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 6 506-513 (8 pages)
Clubroot is one of the most economically important diseases of the Brassicaceae family. Clubroot disease is caused by the obligate parasite Plasmodiophora brassicae, which is difficult to study because it is nonculturable in the laboratory and its races are genetically variable worldwide. In Korea, there are at least five races that belongs to four pathotype groups. A recent study conducted in Korea attempted to develop molecular markers based on ribosomal DNA polymorphism to detect P. brassicae... -
Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants
Chung Youl Park, Hyun-Geun Min, Hong-Kyu Lee, Rameswor Maharjan, Youngnam Yoon, Su-Heon Lee 한국식물병리학회 식물병연구 5 Pages
한국식물병리학회 식물병연구 2018, 24권 4호 15 348-352 (5 pages)
and azuki bean in Korea. Species-specific primer sets were designed for the detection of each virus, and their specificity and sensitivity were tested using mixed primer sets. From among the designed primer sets, two combinations were selected and further evaluated to estimate the detection limits of uniplex, duplex, and multiplex RT-PCR. The multiplex RT-PCR assay could be a useful tool for the field survey of plant viruses and the rapid detection of ClYVV, PeMoV, and TSWV in leguminous plants. -
Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants
Chung Youl Park, Jeong-ran Park, Geun-sik Lee, Seung-In Yi, Byeong Hoon Kim, Jung Sik Eom, Som Gyeol Lee, Hong-sup Kim 한국식물병리학회 식물병연구 4 Pages
한국식물병리학회 식물병연구 2018, 24권 3호 7 233-236 (4 pages)
A multiplex RT-PCR (mRT-PCR) assay was developed for the detection of the recently reported viruses, Cherry virus A (CVA), Little cherry virus 1 (LChV-1), and Little cherry virus 2 (LChV-2), in cherry plants in Korea. Eight sets of primers were designed for each virus and their specificity was tested by using various combinations of mixed primer sets. From the designed primer sets, one combination was selected and further evaluated to estimate the optimum temperature and detection limits of the... -
Arthroscopic detection of medial meniscal injury with the use of a joint distractor in small-breed dogs
Joo-ho Kim, Su-Young Heo, Hae-Beom Lee 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 17 515-520 (6 pages)
The goal of the present study was to evaluate the diagnostic value of a joint distractor in arthroscopy in small-breed dogs. Sixty stifle joints, which were collected from thirty cadavers, were used in this study. To simulate different injuries, no medial meniscal tear, a full-thickness vertical longitudinal tear, a partial-thickness vertical longitudinal tear, full- and partial-thickness vertical longitudinal tears, or a peripheral detachment were created on the caudal horn of the medial... -
Sensitization rates of causative allergens for dogs with atopic dermatitis: detection of canine allergen-specific IgE
Min-Hee Kang, Ha-Jung Kim, Hye-Jin Jang, Hee-Myung Park 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 14 545-550 (6 pages)
Allergen-specific IgE serology tests became commercially available in the 1980s. Since then these tests have been widely used to diagnose and treat allergic skin diseases. However, the relationship between a positive reaction and disease occurrence has been controversial. The purpose of this study was to evaluate allergens using a serologic allergy test in dogs with atopic dermatitis (AD). Dogs clinically diagnosed with AD (n=101) were tested using an allergen-specific IgE immunoassay. Among the... -
Detection of Corynebacterium bovis infection in athymic nude mice from a research animal facility in Korea
Tae-Hyoun Kim, Dong-Su Kim, Ju-Hee Han, Seo-Na Chang, Kyung-Sul Kim, Seung-Hyeok Seok, Dong-Jae Kim, Jong-Hwan Park, Jae-Hak Par 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 13 583-586 (4 pages)
Corynebacterium (C.) bovis infection in nude mice causes hyperkeratosis and weight loss and has been reported worldwide but not in Korea. In 2011, nude mice from an animal facility in Korea were found to have white flakes on their dorsal skin. Histopathological testing revealed that the mice had hyperkeratosis and Gram-positive bacteria were found in the skin. We identified isolated bacteria from the skin lesions as C. bovis using PCR and 16S rRNA sequencing. To the best of our knowledge, this... -
Development of a Real-Time Video Image Tracking Algorithm for Incident Detection
오주택, 민준영, 허병도, 김명섭, Oh. Ju-Taek, Min. Joon-Young, Heo. Byung-Do, Kim. Myung-Seob 한국ITS학회 韓國ITS學會 論文誌 12 Pages
한국ITS학회 韓國ITS學會 論文誌 2008, Vol.7 No.4 49-60 (12 pages)
현재 비디오 영상처리시스템(VIPS: Video Image Processing System)은 실시간으로 들어오는 영상정보를 분석하여 유용한 정보를 제공하고, 하나의 카메라로 여러 차로를 동시에 감시할 수 있는 알고리즘으로 교통량, 속도뿐만 아니라 밀도 및 점유율 등 다양한 정보를 제공하나, 안전지대에서는 효과적이지 못한다. 그러나, 영상검지시스템에서 개별차량에 대한 추적시스템으로 개발할 경우 사고 및 차로 변경의 위험요소 감지 등 실시간으로 보다 다양한 정보를 제공할 수가 있다. 본 논문은 컴퓨터비전 기술을 이용하여 개별차량의... -
코로나19 전후 언론이 바라보는 한국 개신교
한국종교교육학회 종교교육학연구 2022, 제 70권 13 239-255 (17 pages)
연구 목적 본 연구의 목적은 코로나19 전후로 언론이 보도한 한국 개신교의 이미지 변화를 분석적으로 살펴보는 것이다. 연구 내용 본 연구는 최근 4년간 개신교 신문(가스펠투데이)을 통해 매주 보도된 주간 분석 리포터 194회분을 대상으로 종단적인 연구를 진행하였다. 주간 분석리포터는 네이버 뉴스검 색을 통한 내용분석 그리고 빅카인즈를 통한 빅데이터 분석을 함께 진행하였고, 본 연구에서 는 이를 통합적으로 재분석하는 방법으로 결과물을 도출하였다. 결론 연구결과는 첫째, 코로나19는 7배가 넘는 기사량의 급증을...
