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21.

Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine

Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 11 99-109 (11 pages)

Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 저
...
- 4,000원
porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine in piglets. Eukaryotic expression plasmids pcDNA-ORF5, pcDNA-IL-2, and pcDNA-IL-4 were constructed and then expressed in Marc-145 cells. The effects of these genes were detected using an indirect immunofluorescent assay and reverse transcription polymerase chain reaction (RT-PCR). Characteristic fluorescence was observed at different times after pcDNA- ORF5 was expressed in the Marc-145 cells, and PCR products corresponding to...
22.

Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay

Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 5 143-150 (8 pages)

Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 저
...
- 3,000원
(0 to 1,000 mg/kg) ranged from 68.34 to 95.49% (CV; 4.10 to 13.38%). DON in a buffer solution (250, 500 and 1,000 ng/mL) was isolated using 300 μg of NVRQS-DON and 3 mg of magnetic nanoparticles (MNPs). The mean recovery rates of DON using this mAb-MNP system were 75.2, 96.9, and 88.1% in a buffer solution spiked with DON (250, 500, and 1,000 ng/mL). Conclusively we developed competitive ELISAs for detecting DON in animal feed and created a new tool for DON extraction using mAb-coupled MNPs.
23.

Simultaneous detection and subtyping of porcine endogenous retroviruses proviral DNA using the dual priming oligonucleotide system

Hyoung-Joon Moon, Seong-Jun Park, Hye-Kwon Kim, Soo-Kyung Ann, Semi Rho, Hyun-Ok Keum, Bong-Kyun Park* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 14 269-271 (3 pages)

Hyoung-Joon Moon, Seong-Jun Park, Hye-Kwon Kim, Soo-Kyung Ann, Semi Rho, Hyun-Ok Keum, Bong-Kyun Park* 저
...
- 1,500원
detected the PERV proviral genes pol, envA, envB, envC, and porcine mitochondrial DNA only in samples of pig origin. The sensitivity of the primer was demonstrated by simultaneous amplification of all 5 target genes in as little as 10 pg of pig DNA containing PERV proviral genes and mitochondrial DNA. The multiplex PCR, when applied to field samples, simultaneously and successfully amplified PERV proviral genes from liver, blood and hair root samples. Thus, the multiplex PCR developed in the...
24.

Inhibitory effects of interleukin-10 plasmid DNA on the development of atopic dermatitis-like skin lesions in NC/Nga mice

Bock-Gie Jung, Sun-Ju Cho, Jae-Hyung Ko, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 6 213-220 (8 pages)

Bock-Gie Jung, Sun-Ju Cho, Jae-Hyung Ko, Bong-Joo Lee* 저
...
- 3,000원
1 and Th2 cells. Previous studies have reported that IL-10 can ameliorate various inflammatory disorders. The present study was performed to examine whether IL-10 plasmid DNA could suppress development of atopic dermatitis (AD)-like skin lesions in NC/Nga mice, as an initial step towards the development of an appliance for use in dogs with AD. Intradermal injection of IL-10 plasmid DNA markedly inhibited the development of AD-like skin lesions, as evidenced by a marked decrease in skin symptoms...
25.

Parentage testing of Thoroughbred horse in Korea using microsatellite DNA typing

Sun-young Lee, Gil-jae Cho 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 11 63-67 (5 pages)

Sun-young Lee, Gil-jae Cho 저
...
- 1,500원
sires and 314 dams for individual identification were genotyped.Genomic DNA was extracted from 5 hair roots and genotyped by using 14 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HMS1, HMS3, HMS6, HMS7, HTG4, HTG10, LEX3 and VHL20). This method consisted of multiplexing PCR procedure and showed reasonable amplification of all PCR products. Genotypes were determined by genetic analyzer. The number of alleles per locus varied from 3 to 9 with a mean value of 6.36 in TB horse.The...
26.

Microbiology,Immunology : Quantification and genotyping of PCV2 DNA in the tissues of PCV2-infected conventional pigs with different clinical signs

Bong Kyun Park , Seong Jun Park , Hye Kwon Kim , Yu Zi Luo , Hyoung Joon Moon , Se Mi Rho , Jae Yeon Han , Van Giap Nguy 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages

대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2011, 제 51권 제 1호 2 7-14 (8 pages)

Bong Kyun Park , Seong Jun Park , Hye Kwon Kim , Yu Zi Luo , Hyoung Joon Moon , Se Mi Rho , Jae Yeon Han , Van Giap Nguy 저
...
- 3,000원
27.

Knockdown of RCAN1.4 Increases Susceptibility to FAS-mediated and DNA-damage-induced Apoptosis by Upregulation of p53 Expression

YoungSunKim, HongJoonLee, ChorongJang, Ho-ShikKim, , Young-JinCho 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages

대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.6 12 483-489 (7 pages)

YoungSunKim, HongJoonLee, ChorongJang, Ho-ShikKim, , Young-JinCho 저
...
- 3,000원
Despite the potential importance of the human regulator of calcineurin 1 (RCAN-1) gene in the modulation of cell survival under stress, little is known about its role in death-inducing signal pathways. In this study, we addressed the effects of RCAN1.4 knockdown on cellular susceptibility to apoptosis and the activation of death pathway proteins. Transfection of siRNAs against RCAN1.4 resulted in enhanced Fas- and etoposide-induced apoptosis, which was associated with increased expression and...
28.

Rifampicin Inhibits the LPS-induced Expression of Toll-like Receptor 2 via the Suppression of NF-κB DNA-binding Activity in RAW 264.7 Cells

SeongKeunKim, YoungMiKim, ChungEunYeum, Song-HyoJin, GueTaeChae, Seong-BeomLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages

대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.6 11 475-482 (8 pages)

SeongKeunKim, YoungMiKim, ChungEunYeum, Song-HyoJin, GueTaeChae, Seong-BeomLee 저
...
- 3,000원
is a major transcription factor that regulates genes for TLR2 and TNF-Ձ, we examined the effect of rifampicin on the LPS-induced NF-ՊB activation. Rifampicin inhibited NF-ՊB DNA-binding activity in LPS-activated RAW 264.7 cells, while it did not affect IKKՁ/Ղ activity. However, rifampicin slightly inhibited the nuclear translocation of NF-ՊB p65. In addition, rifampicin increased physical interaction between pregnane X receptor, a receptor for rifampicin, and...
29.

The Regulation of AP-1 DNA Binding Activity by Long-term Nicotine Stimulation in Bovine Adrenal Medullary Chromaffin Cells Role of Second Messengers

Jin-KooLee, Seong-SooChoi, Hong-WonSuh 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages

대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.2 7 109-112 (4 pages)

Jin-KooLee, Seong-SooChoi, Hong-WonSuh 저
...
- 1,500원
of AP-1 DNA binding activity in long-term nicotine stimulated bovine adrenal medullary chromaffin (BAMC) cells have not been well characterized. To understand the involvement of second messengers in the regulation of AP-1 DNA binding activity, the present study was designed to define the time-course for inhibition of nicotine-induced responses by cholinergic antagonists, Ca2⁢ and calmodulin (CaM) antagonists, and calcium/calmodulin-dependent protein kinase (CaMK) II inhibitor using...
30.

Epinephrine 합성효소인 phenylethanolamine N-methyltransferase의 인간 genomic DNA의 유전자 크로닝

서유현(Yoo Hun Suh), 허성오(Sung Oh Huh), 전양숙(Yang Sook Chun), 김현식(Hun Sik Kim), 임정규(Jung Kyoo Lim), 박찬웅(Chan Woong Park) 대한약리학회 대한약리학잡지 10 Pages

대한약리학회 대한약리학잡지 1988, 제 24권 제 1호 1 1-10 (10 pages)

서유현(Yoo Hun Suh), 허성오(Sung Oh Huh), 전양숙(Yang Sook Chun), 김현식(Hun Sik Kim), 임정규(Jung Kyoo Lim), 박찬웅(Chan Woong Park) 저
...
- 3,000원
외에는 아직까지 인간 PNMT cDNA나, 전체 genomic DNA의 분리 보고는 없다. 이에 저자들은 인간 PNMT유전자의 전체구조와 여러 종(species) 사이의 진화적인 관계를 규명하기 위해서 human genomic library(Charon 4A)를 만들고, 이 library 이용하여 bovine cDNA를 probe로 13.1 Kb길이의 genomic clone을 분리 크로닝하는데 성공하였다. 이 유전자는 두개의 EcoRI site가 포함되어 있어서, EcoRI제한효소에 의해서 7.5 Kb, 5.0 Kb,0.6 Kb로 분리되었으며, Southern과 dot blot 실험 에서 보면 5.0 Kb와 0.6 Kb에 exon이 흩어져 존재하고...

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