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- JOURNAL OF VETERINARY SCIENCE(31)
- 대한수의학회 학술대회발표집(8)
- KOREAN JOURNAL OF VETERINARY RESEARCH(구 대한수의학회지)(5)
- THE KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY(3)
- THE PLANT PATHOLOGY JOURNAL (2)
- 대한생리학회지(2)
- 대한약리학잡지(2)
- ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES(1)
- THE KOREAN JOURNAL OF ORTHODONTICS(1)
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Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus
Wei Sun, Xiaokai Song, Ruofeng Yan, Lixin Xu, Xiangrui Li* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 8 49-58 (10 pages)
The complete coding sequence of Haemonchus (H.) contortus HC29 cDNA was generated by rapid amplification of cDNA ends in combination with PCR using primers targeting the 5´- and 3´-ends of the partial mRNA sequence. The cloned HC29 cDNA was shown to be 1,113 bp in size with an open reading frame of 507 bp, encoding a protein of 168 amino acid with a calculated molecular mass of 18.9 kDa. Amino acid sequence analysis revealed that the cloned HC29 cDNA contained the conserved catalytic triad and... -
Transmission electron microscopy for characterization of acrosomal damage after Percoll gradient centrifugation of cryopreserved bovine spermatozoa
Letícia Z. Oliveira*, Vera F. M. Hossepian de Lima, Marcelo A. Levenhagen, Ricarda M. dos Santos, Terezinha I. Assumpç 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 10 267-272 (6 pages)
The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three ejaculates per bull were evaluated. Frozen semen samples were thawed and the acrosomal region of sperm cells was evaluated by transmission electron microscopy (TEM) before (n = 18) and after (n = 18) Percoll centrifugation. The evaluation of 20 sperm heads from each of the... -
In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes
Momena Khatun, Mohammad Musharraf Uddin Bhuiyan, Jalal Uddin Ahmed, Aminul Haque, Mohammad Bozlur Rahman*, Mohammed Shamsuddin 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 11 75-82 (8 pages)
p ＞ 0.05). Furthermore, sperm capacitation by heparin alone or in combination with ionomycin did not lead to a significant increase in the normal fertilization rate (34.8 ± 1.7 vs. 32.2 ± 1.5%, respectively) in the oocytes of pubertal goats. In conclusion, the ovaries of pubertal black Bengal goats obtained from the slaughterhouse could be used for in vitro embryo production. However, further optimization of the IVM and IVF techniques are necessary for satisfactory in vitro embryo production. -
Production of cloned sei whale (Balaenoptera borealis) embryos by interspecies somatic cell nuclear transfer using enucleated pig oocytes
Eunsong Lee, Mohammad Musharraf Uddin Bhuiyan, Hiroyuki Watanabe, Kohji Matsuoka, Yoshihiro Fujise, Hajime Ishikawa, Yutaka Fuku 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 2 285-292 (8 pages)
In this study, we examined the feasibility of using subzonal cell injection with electrofusion for interspecies somatic cell nuclear transfer (iSCNT) to produce sei whale embryos and to improve their developmental capacity by investigating the effect of osmolarity and macromolecules in the culture medium on the in vitro developmental capacity. Hybrid embryos produced by the electrofusion of fetal whale fibroblasts with enucleated porcine oocytes were cultured in modified porcine zygote medium-3... -
A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen
Hae-Lee Lee, Sue-Hee Kim, Dong-Beom Ji, Yong-Jun Kim* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 11 249-255 (7 pages)
were evaluated before and after separation. Also, cleavage and blastocyst developmental rate were investigated after IVF with sperm recovered by each separation technique. The motility of samples obtained by the three separation techniques were greater compared to the control samples (p ＜ 0.05). The percentage of spermatozoa with intact plasma-membrane integrity, identified by 6-carboxyfluoresceindiacetate/ propidium iodide fluorescent staining and the hypo-osmotic swelling test, was highest in... -
Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification
B. M. Manjunatha*, J. P. Ravindra, P. S. P. Gupta, M. Devaraj, T. G. Honnappa, A. Krishnaswamy 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 9 153-156 (4 pages)
The present study was conducted to examine post-thaw in vitro developmental competence of buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification. In vitro produced embryos were incubated with a medium containing cytochalasin-b (cyto-b) in a CO2 incubator for 40 min for microfilament stabilization and were cryopreserved by a two-step vitrification method at 24C in the presence of cyto-b. Initially, the embryos were exposed to 10% ethylene glycol (EG) and 10%... -
Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-g
Jeong Ho Park, Haan Woo Sung, Byung Il Yoon, Hyuk Moo Kwon* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 6 131-140 (10 pages)
The aim of this study was to examine the efficacy of in ovo prime-boost vaccination against infectious bursal disease virus (IBDV) using a DNA vaccine to prime in ovo followed by a killed-vaccine boost post hatching. In addition, the adjuvant effects of plasmid-encoded chicken interleukin-2 and chicken interferon-g were tested in conjunction with the vaccine. A plasmid DNA vaccine (pcDNA-VP243) encoding the VP2, VP4, and VP3 proteins of the very virulent IBDV (vvIBDV) SH/92 strain was injected... -
Protective efficacy of commercial inactivated Newcastle disease virus vaccines in chickens against a recent Korean epizootic strain
Woo-Jin Jeon, Eun-Kyoung Lee, Young-Jeong Lee, Ok-Mi Jeong, Yong-Joo Kim, Jun-Hun Kwon, Kang-Seuk Choi 대한수의학회 Journal of Veterinary Science
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 10 295-294 (0 pages)
Despite the intensive vaccination policy that has been put in place to control Newcastle disease virus (NDV), the recent emergence of NDV genotype VII strains in Korea has led to significant economic losses in the poultry industry. We assessed the ability of inactivated, oil-emulsion vaccines derived from La Sota or Ulster 2C NDV strains to protect chickens from challenge with Kr-005/00, which is a recently isolated Korean epizootic genotype VII strain. Six-week-old SPF chickens were vaccinated... -
Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress
Mi Na Lee, Sang Hun Lee, Min Young Lee, Yun Hee Kim, Jae Hong Park, Jung Min Ryu, Seung Pil Yun, Yu Jin Lee, Mi Ok Kim, Kwangsun 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 4 247-256 (10 pages)
Oxidative stresses induced by reactive oxygen species (ROS) have been shown to be involved in several physiological and pathophysiological processes, such as cell proliferation and differentiation. Steroid hormones can protect cells against apoptosis or induce cell proliferation by several mechanisms. Among androgenic hormones, dihydrotestosterone (DHT) is generated by a 5a- reduction of testosterone. Unlike testosterone, DHT cannot be aromatized to estradiol, therefore DHT is considered a pure... -
Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal
Eunsong Lee*, Kilyoung Song 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 15 415-422 (8 pages)
The objective of the present study was to examine the feasibility of the production of autologous porcine somatic cell nuclear transfer (SCNT) blastocysts using oocytes and donor cells from slaughtered ovaries. Therefore, we attempted to optimize autologous SCNT by examining the effects of electrical fusion conditions and donor cell type on cell fusion and the development of SCNT embryos. Four types of donor cells were used: 1) denuded cumulus cells (DCCs) collected from in vitro-matured (IVM)...
